Name: ________________________________________ Date: __________________ AP Biology – Continuous Quiz-M - PLANTS 1. Water and minerals in the soil have two ways to enter the roots called the apoplastic route and the symplastic route. The former describes the solution traveling through the cell walls, while the later describes the solution moving through the cytosolic of cells and passing from cell to cell via plasmodesmata. Sometimes the solution can switch from one route to the other via the transmembrane route. However, in the end, all solutions must enter the symplastic route when it hits the layer of cells just outside the xylem and phloem called the endodermis thanks to the impermeable casparian strip in its cell wall. Therefore the solution MUST cross the cell membrane now and this is what gives the plant strict control over what enters. 2. Transpiration is the process used by plants to move water and minerals from the soil upward to the rest of the plant through the xylem, which are dead, hollowed out cells through which the water moves. Ultimately, the water will leave through the stomates in the leaves, which are governed by guard cells. The solution moves solely by the process of diffusion and since we are talking water it is more specifically called osmosis. Therefore, the water is always moving from a high water potential in the soil to a very low water potential in the air. The reason it is so low in the air is because the air is mostly nitrogen and has a very low water concentration. None of this would be possible without two very important properties of water, namely cohesion (water bonding to water) and adhesion (water bonding to the inside of xylem cells). This is because as water evaporates from the leaves it will pull on neighboring water molecules via their hydrogen bonds, and the same bonds are used to grab the xylem cells to prevent back flow down the plant. This process is known as the cohesion-tension hypothesis. 3. Describe how guard cells open and close stomates in leaves: Guard cells will open when there is sunlight and available water to allow transpiration and gas exchange. They achieve this by pumping potassium (K+) either into or out of the cell. If they pump K+ in then the water potential of the cytosol will drop and be less than the extracellular fluid. This will cause water to diffuse into the cell resulting in turgor pressure and the stomata will open. If K+ is pumped out of the cell then the extracellular fluid will have the lower water potential and water will diffuse out and the cells will be flaccid resulting in closed stomata. 4. Four factors that influence the rate of transpiration in plants are light, humidity, wind, and temperature. State whether the factor increases or decreases the rate of transpiration and justify your answer. Process Rate (Increase/Decrease) Light Increase Humidity (increasing) Decrease Wind Increase Temperature (increasing) Increase Justification More light mean more stomata will open and transpiration will increase up until a saturation point where opening more beyond this will make no difference. As humidity increases the air accepts fewer water molecules reducing the rate of evaporation from the leaves. Wind will move the humid air around the leaves out of the way and displace it with less humid allowing for evaporation rates to increase. The kinetic energy of the water molecules will increase making diffusion/osmosis and evaporation (liquid to gas) quicker. 5. Compare mycorrhizae to rhizobium. Mycorrhizae: Fungus that grows in association with the roots of plants in a usually mutualistic relationship where the hyphae of the fungus extend the roots to obtain more water and nutrients while the plant supplies the fungus with organic materials (food). Rhizobium: Genus of nitrogen fixing bacteria that live in the root nodules of legumes (plants with pods like peas, peanuts, clover, etc…) in a mutualistic relationship. The plants get NH3 from the bacteria and the bacteria get organics (food) from the plant. 6. Some plants are considered “carnivorous”. They still do photosynthesis like any other plant to make their food. For what reason are they “carnivorous” then? They live in soil that is low in particular minerals and the animals (usually insects) contain these minerals. 7. Define circadian rhythm and give on example in humans and one in plants. Circadian Rhythm: roughly 24hour cycles in organisms including plants and animals Humans- hunger, sleep, etc… Plants- flower opening and closing, leaf movement, stomatal opening and closing Name: ________________________________________ Date: __________________ AP Biology – Continuous Quiz-N - PLANTS 1. a) In contrast to transpiration, plants move organic molecules like sucrose up and down through the phloam cells, which are living unlike xylem cells, using the process of Translocation. The direction depends on the time of the year. In early spring when leaves are being made net flow will be upward and in summer when photosynthesis is at its maximum it will be downward. The mechanism behind this process is known as pressure flow. The place where the sugar is coming from (either roots or leaves depending on the time of the year) is known as the sugar source, and the place the sugar is going is known as the sugar sink. b) Show how this process works using a labeled figure: 2. Plants, like humans, use a number of different hormones to coordinate their many cells to work as a single entity. One such class of hormones is called auxin. The specific naturally occur member of this class is indoleacetic acid (IAA). They are heavily involved in regulating the growth of shoots (stems) toward light, a process known in general as phototropism. Since it is growing TOWARD light we more specifically call it positive phototropism as opposed to growing AWAY from light, which is called negative phototropism. This is in contrast to say an animal moving (locomoting) toward or away from light, which is called phototaxis. This hormone class is produced and secreted from apical meristem located at the tips of growing shoots. The way these hormones cause the growth toward light is by activating proton pumps, which pump protons out of the cell causing the pH around the cell wall to decrease in cells on the shaded side of the shoots only. This weakens the cell wall and causes these cells to get longer, which does not occur to the cells on the side of the stem being hit with light. The result is that the stem bends toward the light source. This process was first described by none other than Charles Darwin (and son) in 1880. 3. Identify three other tropisms and describe each. Include and circle the one that auxin is also thought to play a role in stimulating: Tropism Description Geotropism (involves auxin) Thigmotropism Growth in response to gravity (roots exhibit positive geotropism) Hydrotropism Growth in response to presence of water (roots exhibit positive hydrotropism) Growth in response to touch (vines wrapping around objects) 4. Plants can sense light/darkness using cytosolic (location) receptor proteins known as phytochromes, which contain a light-sensing photoreceptor domain and an enzymatic kinase domain. The former domain, in order to sense light (which means the domain must undergo a conformational change when light hits it) has a cofactor called a chromophore that is able to absorb red (color) light. The receptors exist in two forms, Pr and Pfr, that can switch back a forth depending on the wavelength of light hitting the protein. In the dark, the receptors are in the Pfr form, but when hit with red (color) light they switch to the active Pfr form. The result is signal transduction, which likely involves a phosphorylation cascade allowing for signal amplification followed by activation of transcription factors to alter gene expression resulting in cellular responses like plastid differentiation to chloroplasts. The ratio of the two receptor forms to each other in the cells allows the plant to determine levels of light through the day and year. These pathways are no different from the cell signaling pathways we have already seen accept the “ligand” in this case is light. The same three general stages occur, namely reception, transduction, and response. Name: ________________________________________ Date: __________________ AP Biology – Continuous Quiz- O - PLANTS 5. a) Define Photoperiodism The response of an organism to the length of day or night – the photoperiod is the length of daylight/darkness in a day. For example an 8:16 photoperiod is 8 hours day and 16 hours night. b) Compare short day, long day and day-neutral plants Short-Day Plants that flower only when nights are longer than an uninterrupted critical period (days are short). Long-Day Plants that flower only when nights are shorter than a particular critical length (days are long) Day-Neutral These plants don’t care…they flower regardless of day length c) Look at the figure below showing an experiment done on short day plants. What does this indicate the plants are actually measuring and why? In this experiment, group one show the plants flowering in an 8:16 photoperiod. Group two reveal that if the daytime is extended to 16 hours (16:8 photoperiod) then no flowering occurs. Group three, however, shows that if the plants were measuring day length then they should have measured 8 hours of day regardless of the night pulse, but they don’t flower. Therefore, they are likely measuring the length of the dark period.
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