LED Stimulator (Four (+1) channel ‘ganzfeld’ unit) Operators Manual CH Electronics 27 Calmont Road, Bromley, Kent, BR1 4BY UK Tel/.Fax. +44 [0]181 460 7809 LED4 User manual 1 Contents 1.0 Introduction 3 2.0 Description 6 2.1 Controller 6 2.2 Head stage 7 3.0 Controls and connections 8 Panel layout 10 4.0 Limitations 11 4.0 Calibration 12 4.1 Frequency display 12 4.2 Light intensity 12 Circuit board layouts 13 5.3 Calibration as shipped 15 5.4 Spectral output 16 6.0 Computer control 17 7.0 Modifications from standard 19 LED4 User manual 2 1.0 Introduction 1.1 Light emitting diodes or LED's are near ideal light sources for many purposes. They are small, require low voltages and currents to drive them, can be controlled by simple electronic means to give either continuous light outputs or extremely brief flashes (or both together) over a large range of intensities. Their light output changes by less than 10% in intensity or relative spectral emission over 50,000 hours of use (Hewlett Packard application note 1017). These properties not only simplify calibration but also reduce the frequency with which it is needed. Many different wavelengths of light are available, in a variety of different physical packages with differing optical properties. The majority of the available devices are inexpensive. The chief difficulties users are likely to experience are due to the low intrinsic source brightness, which is considerably less than that of arc or incandescent sources. Nevertheless, even where considerable quantities of light are required (for example in electroretinography) LED’s can be used to advantage. Light emitting diodes or LED's are members of the family of epitaxial semiconductor junction diodes. Growing a very thin crystal of a semiconductor directly onto another, slightly different semiconductor surface forms a junction. The two layers of semiconductor material, (frequently gallium aluminum arsenide) each contain different impurities (dopants). As a result of these impurities, one layer contains an excess of free electrons and the other an excess of holes (positive charge). The energy required to move a charge across the junction against the concentration gradient of free electrons or holes is considerable, and larger than in other types of diode, (approx. 3:1). This energy "band-gap" must be exceeded if current is to be passed through the junction. When the device is forward biased electrons move from the negative material to the positive and a corresponding movement of positive charge or "holes" occurs in the reverse direction. When an electron and hole pair recombines, its energy is emitted as a photon. The characteristic wavelength of the photon is given by the energy band gap. Thus, it is more difficult to produce short-wavelength LED’s, since the higher energy gap must be maintained with the controlled flow of charges. The construction of the epitaxial layer determines the direction of light emitted, and the absence of a resonating (reflective) cavity prevents the stimulated emission of radiation by the photons, so the light is not coherent, and contains a number of differing wavelengths. However, light is emitted over a relatively narrow bandwidth. For a typical red led, (for illustrative purposes a Stanley HBR5566X) the peak is at 660nm and the half-power bandwidth is +/- 30nm. The construction of a 'typical' LED is shown in figure 1. The semiconductor is mounted on a "lead frame", and encapsulated in a plastic (epoxy) housing with an internal spherical lens. The combination of junction structure, epoxy and lens type determine the spatial output characteristics of the device and for many LED’s, including all the "brightest", the light is concentrated in a cone, which can be represented in a polar diagram (figure 2), with a half-power spatial / polar distribution of +/- 7.5 degrees. A typical device requires fifty milliamps at around two volts to produce its maximum output, so it is both easy to control and intrinsically safe to use in a clinical environment. The junction of most modern devices thus exhibit a low electrical impedance, but are nevertheless fairly robust, being able to tolerate significant overloads for short periods. LED4 User manual 3 Figure 1 Various types of LED are available. Some are devised for special purposes, for example alphanumeric display components or indicators. There is a considerable range of shape, size and intensity. A number of devices are packaged with additional circuitry that provides a constant current flow or flash the device on and off. Other devices contain more than one junction and can produce two or even three colours, but these are so specialised that their value for purposes other than those for which they were designed, that is for instrument displays, is limited. However, some squarepackaged arrays may be useful for producing checkerboards or similar displays. The colours quoted by manufacturers vary from infrared to blue, but this is may be misleading. In general, red LEDS are by far the brightest, and have peak emission at around 660nm. Yellow LED’s are also efficient light emitters, and peak at c. 580 nm, so that a variety of oranges can be obtained. Green LED’s are now available with a considerable light output. However, all have peak emission at c. 560nm, which, it should be remembered, is the peak of the photopic relative spectral sensitivity curve. This characteristic is unfortunate, since "green" light is often thought to selectively stimulate rods. Various filters are also incorporated in the plastic capsules of green LED’s to remove the longer wavelengths, but none provides a filter effective enough to modify the output in any significant manner. If additional external filters are employed, it will be found that the proportion of light emitted below 510nm is so low, (<5%) that it is difficult to obtain a blue-green light of adequate intensity for most electrophysiology. Blue LED’s are currently available from several manufacturers and are significantly different to all others. They generally have considerably less light output, and the voltage required at the junction is significantly higher as is the junction capacitance. The peak of the light output is at 480 nm, but the bandwidth is wider than that of many other devices and asymmetrical, being broader towards the longer wavelengths. The relative spectral emission varies somewhat with the drive current. The devices are also much more costly and 'fragile'. A single red LED may cost a few pence; a blue LED with a much lower output costs around twenty pounds. Nevertheless, blue LED’s are very useful in electrophysiology and psychophysics, and with suitable filtering can provide stimuli of wavelength as low as 420nm or extending to as high as 520 nm: thus, LED’s can be used for many purposes previously requiring incandescent sources and monochromators or interference filters. The relationship between applied current (or voltage) and light output of a typical device is shown in figure 2. And for a region of about one and one half decades can be seen to be approximately linear. Above or below this region, marked non-linearity occurs. LED4 User manual 4 Since in general, users wish to control intensity in a simple ergonomic way over a much larger range, a variety of drive circuits has been devised. For a simple flash stimulator, a voltage drive circuit may be used quite effectively, and the relationship between light output and applied voltage determined by calibration. Figure 2 LED charcteristics 5 2.3 2.2 4 Forward voltage 2 3 1.9 1.8 2 1.7 1.6 1 1.5 1.4 0 0.1 0.2 0.5 1 2 5 10 Current (mA) Vf LED4 User manual Intensity 5 20 50 100 Relative intensity (20mA) 2.1 2.0 Description 2.1 Controller The stimulator is designed to deliver square pulses of light rather than complex waveforms, and as such is able to use a simple current pulse drive, Additional intensity control is provided by means of pulse width control, this is precisely variable over a range of microseconds to tens of seconds. The stimulus waveform is generated by a free running oscillator, this oscillator triggers a monostable pulse generator. The pulse generator is formed from a crystal oscillator, the output of which is divided down by a series of counters to provide decade steps. The output of this stage is then divided by a second series of counters giving steps of one through fifteen. The output of this waveform generator is connected to the output driver stages. The four output driver stages share a common architecture. A pre-programmed power (voltage) source, delivers power through a fixed resistor. The programmed element being set using one of seven internal pre-set controls or one continuously variable potentiometer to determine the attenuated levels by controlling the drive voltage. The waveform signal drives a switching device which allows the pre-set current to pass through the LED’s. Figure 3 LED4 User manual 6 2.2 Head Stage The four groups of LED’s are mounted on a small planar circuit board with current limiting resistors. This circuit board is mounted at one end of an internally partially mirrored plastic tube (160mm long x 50mm diameter), with a diffusing screen approximately mid way along the tube and a hemispheric translucent diffuser mounted at the far end of the tube. On older versions of the instrument, a fixation spot is provided via a 0.8 mm plastic optical fibre mounted through the rear surface of the hemisphere. This fixation spot is not installed on later versions, although it can be retro fitted if required. A white neoprene ‘eye cup’ assures patient comfort. The complete assembly is mounted on ball joint assembly to ensure correct fitting to a wide range of facial shapes (figure 5 shows a dual stimulator system). Figure 4 Figure 5 LED4 User manual 7 3.0 Controls (See panel layout schematics at end of section) 3.1 Front Panel 1 Variable frequency control. Sets the frequency of the base oscillator for the waveform (pulse) generator. Frequency control range switch. Sets the range controlled by (1). The three position switch selects a high and low frequency range, and in the third position, which selects the higher frequency range, bypasses the pulse width control circuits to provide a 50% duty cycle drive waveform. Run. Starts and stops the waveform (pulse) generator. The set frequency is still displayed when the output is gated using this control. In the ‘off’ position, external signals via (24) set the frequency, or pulses initiated with push button (4) are output. Single shot manual pulse trigger. Pulse width range. Determines the multiplier setting for the output pulse. Range 1uS to 10S. Note, pulse widths below 10uS are subject to increased error in light pulse width. Multiplier for (5). Example; If the range is set to 1mS, and this control is set to 12, then a light output pulse of 12mS will be generated. N.B. the 0 setting is marked, but should not be used. A number cannot be divided by zero. Frequency display for the internal waveform generator. Channel 1 / Red Waveform select switch. Selects one of the following; Channel off, Positive going flash, i.e. dark to light, Negative going flash, i.e. light to dark, Channel on, or any of these with a level set by the variable intensity control as the ‘off’ state. Using these settings it is enables a flash to be superimposed on a background of the same colour. N.B. The continuous intensity controls are not linear. See calibration section for details. Channel 1 / Red Stepped Attenuator. Attenuates the light output in steps of 1,3,10…1000, or selects the variable attenuator, (10). (See note in limitations section for relevant notes), Channel 1 / Red Variable Attenuator. Selected by (9). Provides a continuously variable output across the range of the channel. (See note in limitations section for relevant notes), N.B. This control is not linear. See the calibration graph in section 5.1 for details. Channel 2 / Green Waveform select switch. Selects one of the following; Channel off, Positive going flash, i.e. dark to light, Negative going flash, i.e. light to dark, Channel on, or any of these with a level set by the variable intensity control as the ‘off’ state. Using these settings it is enables a flash to be superimposed on a background of the same colour. N.B. The continuous intensity controls are not linear. See calibration section for details. Channel 2 / Green Stepped Attenuator. Attenuates the light output in steps of 1,3,10…1000, or selects the variable attenuator, (13). (See note in limitations section for relevant notes), Channel 2 / Green Variable Attenuator. Selected by (12). Provides a continuously variable output across the range of the channel. (See note in limitations section for relevant notes), N.B. This control is not linear. See the calibration graph in section 5.1 for details. Channel 3 / Blue Waveform select switch. Selects one of the following; Channel off, Positive going flash, i.e. dark to light, Negative going flash, i.e. light to dark, Channel on, or any of these with a level set by the variable intensity control as the ‘off’ state. Using these settings it is enables a flash to be superimposed on a background of the same colour. N.B. The continuous intensity controls are not linear. See calibration section for details. 2 3 4 5 6 7 8 9 10 11 12 13 14 LED4 User manual 8 15 16 17 18 19 20 21 22 Channel 3 / Blue Stepped Attenuator. Attenuates the light output in steps of 1,3,10…1000, or selects the variable attenuator, (16). (See note in limitations section for relevant notes), Channel 3 / Blue Variable Attenuator. Selected by (14). Provides a continuously variable output across the range of the channel. (See note in limitations section for relevant notes), N.B. This control is not linear. See the calibration graph in section 5.1 for details. Channel 4 / Amber Waveform select switch. Selects one of the following; Channel off, Positive going flash, i.e. dark to light, Negative going flash, i.e. light to dark, Channel on, or any of these with a level set by the variable intensity control as the ‘off’ state. Using these settings it is enables a flash to be superimposed on a background of the same colour. N.B. The continuous intensity controls are not linear. See calibration section for details. Channel 4 / Amber [ + white] Stepped Attenuator . Attenuates the light output in steps of 1,3,10…1000, or selects the variable attenuator, (19). (See note in limitations section for relevant notes), Channel 4 / Amber [ + white] Variable Attenuator. Selected by (18). Provides a continuously variable output across the range of the channel. (See note in limitations section for relevant notes), N.B. This control is not linear. See the calibration graph in section 5.1 for details. Power indicator light. (Mains supply on). Channel 4 / 5 select switch. Selects the 5th [white] channel to be controled instead of the amber, using the controls for channel 4. Note; There is no continuously variable control for channel 5. If ‘Var’ is selected, the channel is effectively off. Trigger edge select. Selects leading or trailing edge of the flash on which to output a ttl trigger pulse, (internal waveform generator only), though the Trigger output socket, (23). 3.2 Rear Panel 23 TTL trigger output socket. Positive going TTL pulse, 0.2mS duration, output under the control of (22). See section on modifications for changes. Trigger input. When (3) is in the ‘off/ext.’ position, a pulse applied to this connector initiates an output as set by controls 5 – 19. Not fitted. Not fitted. Instrument ground connection. Connected to mains earth via the IEC power lead. N.B. Not for patient connection. …. Stimulator head connector. [25 way ‘D’ socket] Connection to Ganzfeld heads. Note that >30 volts is present on this connector, connection to incorrect equipment will cause damage. Mains power switch. IEC inlet socket. Fitted with two one amp 230 volt fuses. Normally set for 230V 50Hz input. See modifications section for alternate power supplies. Stimulus head connection. NB. Use only for the head supplied with the controller. The head stages, (ganzfelds), are not interchangeable. Swapping heads between unit may cause serious damage to either component. Mains power ON / OFF switch. Dual fused IEC mains power inlet connector. Use only fuses as supplied. (1 amp 230 volt, fast blow). 24 25 26 27 28 29 30 31 32 LED4 User manual 9 LED4 User manual 10 4.0 Limitations Maximum intensities; All LED’s, especially those of longer wavelength emission, are subject to a heating effect, where, when driven at high (approaching maximum) the light output intensity falls as the junction heats. For this reason, it is recommended that the red channel is are not used for pulses of more than 100msecs duration, or backgrounds at attenuation settings of 1, or the variable control equivalent. These levels will not damage the devices, but a reduction of output with time will occur. This heating effect will especially in the shorter wavelength devices, cause a change in the wavelength of the emitted light. These changes are small even for the blue devices, where they are of the order of 3 to 5nm across the entire range of intensities. LED4 User manual 11 5.0 Calibration. Adjustment. Please note that all adjustments described should only be carried out by suitably qualified persons. If in doubt refer back to the manufacturers. 5.1.1 Frequency display. The one second base interval for the counter can be adjusted as follows. 5.1.2 Switch the power off and disconnect the power supply. 5.1.3 Turn the controller over and remove the 4 screw-on feet. The base may now be removed. 5.1.4 Reconnect the power supply, 5.1.5 Set the frequency to around 10Hz and using suitable instrumentation measure the output frequency at pin X on XX. Adjust the pre-set potentiometer XX so that the display coincides with measured frequency. 5.1.6 Repeat the comparison at the upper and lower end of the frequency output. 5.1.7 Disconnect the power and reassemble the unit. 5.2.0 Light output. 5.2.1 Switch the power off and disconnect the power supply. 5.2.2 Turn the controller over and remove the 4 screw-on feet. Turn the case over and remove the top cover. 5.2.3 Reconnect the ganzfeld bowl and power supply. 5.2.4 Place a suitable photometer head against the ganzfeld in such a position as to be in the same plane as the eye of the subject. 5.2.5 Switch the channel to be adjusted to ‘on’ using the waveform selector switch 5.2.6 Set the stepped attenuator to the setting to be adjusted. 5.2.7 Taking care not to exceed the maximum current levels for the channel under test, adjust the appropriate preset, (for locations see ‘driver boards’). LED4 User manual 12 LED4 User manual 13 LED4 User manual 14 6.0 Calibration as shipped Attenuator settings Calibration - 20th January 2003 - IL1700 Fixed Attenuation 1 3 10 30 100 300 1000 Red 278 92.7 27.8 9.3 2.8 0.9 0.3 Green 1916 638.7 191.6 63.9 19.2 6.4 1.9 Blue 31.000 10.333 3.100 1.033 0.310 0.103 0.031 Amber 825 275.0 82.5 27.5 8.3 2.8 0.8 White 1685.0 561.7 168.5 56.2 16.9 5.6 1.7 Spectra file; Cardiff3.wk4 Trig.op. 40uS +ve Continuous Dial 0 50 100 200 300 400 500 600 700 800 900 1000 Red Green 3.78 31.3 62.8 95.6 126.9 160.0 187.4 234.0 268.0 0.178 0.815 9.24 249.0 607.0 907.0 1163.0 1386.0 1584.0 1764.0 1928.0 Blue Amber 0.57 2.74 7.21 11.43 15.36 18.95 22.3 25.5 28.8 31.3 0.005 1.59 83.3 190.6 294 390 462 539 620 703 767 Calibration figures in Cd/m2, measured using IL1700. LED4 User manual 15 White Not available Spectral distribution (attenuation 3) 1 red/3 green/3 blue/3 amber/3 white/3 normalise output 0.8 0.6 0.4 0.2 0 350.1 424.2 387.4 496.6 460.7 567.3 532.2 636.2 602.0 wavelength (nm) LED4 User manual 16 703.4 670.0 768.8 736.3 6.0 Computer control - Not installed Computer control connection is via the paired 37 way ‘D’ connectors on the rear panel. The recommended interface card for computer control is the PC214E from Amplicon Electronics, connection is via the 37 to 78 way ‘D’ cable available as an optional component. Control output; This socket carries lines from the front panel switches. They are connected via the ‘Control input’ socket to the relevant points on the circuit boards. By removing the 37 way bridging connector and connecting the external control cable from the remote control device to the control input socket, the front panel switches are replaced by the external control device. It should be noted that this gives direct access to the CMOS gates of the control devices on the printed circuit boards. All inputs and outputs concerned with external control require TTL compatible signals. The inputs are CMOS gates tied low with 10K ohm resistors. Care must be taken if devices other than the PC214E is used not to deviate from these levels. Exceeding the TTL specifications could cause damage to the stimulator. The required connections are detailed below in the following table. LED4 User manual 17 Description SCREEN Pulse width - period Pulse width - period Pulse width - period Pulse width - multiplier Pulse width - multiplier Pulse width - multiplier Pulse width - multiplier Red - Waveform Red - Waveform Red - Waveform - secondary Red - Attenuator Red - Attenuator Red - Attenuator Green - Waveform Green - Waveform Green - Waveform secondary Green - Attenuator Green - Attenuator Green - Attenuator Blue - Waveform Blue - Waveform Blue - Waveform - secondary Blue - Attenuator Blue - Attenuator Blue - Attenuator Amber - Waveform Amber - Waveform Amber - Waveform secondary Amber - Attenuator Amber - Attenuator Amber - Attenuator Trigger - Select edge EXT select GROUND GROUND GROUND LED4 User manual bin. Pin # 1 2 4 1 2 4 8 1 2 4 1 2 4 1 2 4 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 1 2 4 1 2 4 1 2 4 1 2 4 18 19 20 21 22 23 24 25 26 27 28 29 1 2 4 1 1 30 31 32 33 34 35 36 37 18 7.0 Modifications 7.1 Trigger output has been set to 0.04ms, zero going high, TTL compatible. [May be set internally to +5V going to 0 if required]. 7.2 Power supply; Mains (line) inlet is configured for 230 volts at 50/60Hz and dual fused with 1.0 amp ‘quick blow’ fuses. 7.3 Channel 4 now can be used with either amber of ‘white’ (see spectral output graph). For details see the controls description section. Please Note;Refer all servicing or modifications to suitably qualified person. Any repairs or modifications not specifically approved in writing by CH Electronics may void the warranty on this instrument and could also render it unsafe. LED4 User manual 19
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