DEPARTMENT OF VIROLOGY
Angel S. Galabov, MD, DSc, Professor of Virology,
Corresponding Member of Bulgarian Academy of Sciences
Head of Department
Tel: (+359 2) 870-01-08
E-mail: [email protected]
1939
1962
1968
196869
1967-74
Born February 15, Sredez, Stara Zagora district
MD, Medical Faculty, Medical University of Sofia
PhD in Virology, Institute of Microbiology, BAS
Postdoctoral Fellowship, Institut Pasteur, Paris
Assistant Professor, Department of Virology, Postgraduate Medical School (Medical
Academy), Sofia
1972-88 Head, Viral Inhibitors and Interferon Laboratory, Department of Virology, Institute
of Infectious and Parasitic Diseases, Medical Academy, Sofia
1974Associate Professor, Department of Virology, Medical Academy
88
1975
Visiting Scientist, Belozersky Laboratory of Molecular Biology and Bioorganic
Chemistry, Moscow State University
1978
DSc in Virology, Medical Academy, Sofia
1983World Health Organization Expert Adviser (Viral Diseases Panel)
2006
1988
Professor of Virology, Institute of Microbiology, BAS
1988Head, Department of Virology, Institute of Microbiology, BAS
1992-97 Member of the International Council of the International Society for Antiviral
Resarch
1995Director, The Stephan Angeloff Institute of Microbiology, BAS
1995-02 Member of the Board of the Bulgarian National Academy of Medicine
1996
Awarded by the Russian Academy of Medical Sciences with the Jubilee Medal
"Ivanovsky/100 Years Virology"
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2003
President, Balkan Society for Microbiology (BSM)
National Award “Diploma and Entering the Name in of the Golden Book of
Inventors”
2004
Corresponding Member of the Bulgarian Academy of Sciences
2005
Member of Honor of the Romanian Academy of Medical Sciences
1947
1971
1976
1979
1986
2001
Lyubka Doumanova
PhD, Assoc. Professor
Head of Research Team
Tel: (+359 2) 979 63-56
E-mail:
[email protected]
1946
1970
1974
1978
1988
2003
Born July 30, Sofia
Diploma, Faculty of Chemistry, Sofia
University ”St. K. Ohridski”
PhD in Biochemistry
Assistant Professor
Associate Professor, Central Veterinary
Research Institute, Sofia
Associate Professor, The Stephan Angeloff
Institute of Microbiology, Bulgarian Academy
of Sciences
Scientific Secretary, The Stephan Angeloff
Institute of Microbiology, Bulgarian Academy
of Sciences
Born December 29, Sofia
Diploma, Faculty of Biology, Moskow State University
PhD in Virology
Assistant Professor
Associate Professor
Julia Serkedjieva
PhD, Assoc. Professor
Head of Research Team
Tel: (+359 2) 979 31-13
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E-mail: [email protected]
Staff of the Department of Virology
Lubomira Nikolaeva-Glomb, MD, PhD, Research Scientist
Yuri Abashev, PhD, Research Scientist
Mimi Remichkova, PhD, Research Scientist
Assya Angelova, PhD, Research Scientist
Zahari Raykov, PhD, Research Scientist
Ivanka Nikolova, PhD, Research Scientist
Ralitsa Vassileva-Pencheva, MS, Research Scientist
Lora Simeonova, MS, Research Scientist
Ivana Roeva, DVM, Research Assistant
Lucia Mukova, MS, Research Assistant
Galina Gegova, MS, Research Assistant
Anna Teodossieva, MS, Research Assistant
Maya Gatzovska, MS, Research Assistant
Emilia Popova-Ajvazova, BS, Research Assistant
Krassimira Beshkova, BS, Research Assistant
Snezhana Logofetova, Laboratory Assistant
Kirilka Todorova, Laboratory Assistant
Consultant
Lilia Wassilewa, PhD, Professor
PhD Students
Julian Tumbarski, DVM
Irina Georgieva, MS
Main Research Topics

Viral replication inhibitors - ligands of viral proteins: mode of action, resistance,
combination effects of viral inhibitors

Combined action of viral replication inhibitors and biological response modifiers
(immunomodulators, interferon inducers, antioxidants, etc.)

Search for new viral inhibitors of synthetic and natural (plant, microbial, etc.) origin

Antiviral microbicides

Viruses – diabetes

Human papillomaviruses

Viruses – Balkan endemic nephropathy
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
Cancerolytic effects of parvoviruses

Viral proteins
Most Important Achievements

Discovery of new original antiviral substances effective in the treatment of viral
infections in humans and domestic animals:
- Guanidine derivative - chemotherapeutic agent, inhibiting replication of
adenoviruses, effective in the treatment of epidemic keratoconjunctivitis and other
adenovirus conjunctivitides (widely spread viral infections of the eye). It is the first
known antiadenoviral chemotherapeutic agent.
- Cyclic urea derivatives, inhibiting selectively replication of influenza and
togaviruses.
- Dipyridamole - a large spectrum antiviral agent, acting via induction of endogene
interferon, used for prevention of flu and other acute viral respiratory infections.

Development of rational approaches for selection of synergistic combinations of
antimetabolites effective against herpesviruses, based on targeting of key metabolites dGTP (ribavirin + acyclovir, mycophenolic acid + acyclovir), dTTP (amethopterin +
bromovinyldeoxyuridine) or involving a virus adsorption preventing agent (dextrane
sulfate + acyclovir) and elucidation the mechanisms of antiviral synergism.

Clearing-up of the mode of antiviral action of mopyridone on influenza A virus
replication (binding to the structural M1 protein).

Discovery and characterization of the antiviral effect of cycluridine towards flavivirus
infections, the first chemotherapeutic agent efficacious in the mucosal disease-virus
diarrhea in cattle.

Development of the concept for low-molecular weight interferon inducers as inhibitors
of the cyclic AMP phosphodiesterase, a base for directed selection of active
substances.

Establishment and characterization of antiviral activity of a series of pure substances
of plant origin.

Establishment of synergistic combination effect of rimantadine and oseltamivir against
influenza virus A(H3N2) infection in mice.

Clearing-up of the drug-resistance phenomenon in enterovirus replication inhibitors
interacting with the viral capsid protein VP1 – problems of the development of highly
effective anti-enteroviral chemotherapy. Phenotype and molecular genetic
characteristics and disoxaril resistant and dependent mutants of Coxsackie virus B1.

Discovery of a series of highly efficient synergistic combinations of enteroviral
replication inhibitors – a perspective approach for dissolving the drug-resistance
problem.
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
Establishment of the synergistic combination effect of cidofovir and idoxuridine
against vaccinia virus replication.

Discovery of oxoglaucine: a highly effective and large-spectrum inhibitor of
enterovirus replication.

Elucidation of the protective effect of a plant polyphenol-rich extract in the lethal murine
influenza H3N2 virus infection.

Establishment of the anti-influenza virus effects of novel protease inhibitors from
Streptomyces.

Determination of the role of the oxidative stress in the pathogenesis of flu and the
influence of certain antioxidants of natural and synthetic origin and antivirals on this
process.

Determination of the antiviral spectrum of the highly effective and broad-spectrum
virocidal composition of new type (Manorapid Synergy®), implemented in the clinical
practice as hand disinfectant.

Characterization of the immune status in patients with Balkan endemic nephropathy
(lymphocytes phenotyping, phagocytes activity, neopterin level).

Establishment of data about the role of hantavirus infections in etiopathogenesis of the
Balkan endemic nephropathy,

New proves on the diabetogenic role of neutropic viruses.

Proves on the role of human papillomaviruses in etiology of CIN and cervical cancer
in Bulgarian population (molecular epidemiological study).

Proves on the cancerolytic effect of some parvoviruses.
Selected Publications
Galabov, A. S., A. V. Itkes, M. Mastikova, V. L. Tuniskaya, E. S. Severin (1985):
Dipyridamole-induced interferon production in mouse peritoneal leukocytes. Biochemistry
International, 11, 591-598.
Konstantinov, K., A. Galabov, M. Mastikova (1989): Interferon response to
dipyridamole in lupus erythematosus patients. British J. Dermatology, 121, 59-62.
Pancheva, S. (1991): Potentiating effect of ribavirin on the antiherpes activity of
acyclovir. Antiviral Res., 16, 151-161
Alexandrov, M., R. Peshev, I. Yanchev, S. Bozhkov, L. Doumanova, T. Dimitrov, S.
Zacharieva (1992): Immunohistochemical localization of the rabbit haemorrhagic disease
viral antigen. Arch. Virol. 127, 355-363.
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Galabov, A. S., M. L. Khristova, S. Uzunov, L. Wassileva, D. J. Bucher, I. G.
Kharitonenkov (1994): Alteration in the antigenic structure of M1 protein of influenza A
virus mutant resistant to a new antiviral compound mopiridone. Acta virol., 38, 5-10.
Wassilewa, L., S. Uzunov, A. S. Galabov, A. N. Simonov, S. Markushin, M.
Alexandrov (1995): Characterization of the virions of mopyridone-sensitive wild strain and
mopyridone-resistant mutant of influenza virus A(H3N2). Acta virol., 39, 79-84.
Nikolaeva, L., A. S. Galabov (1995): Synergistic inhibitory effect of enviroxime and
disoxaril on poliovirus type 1 replication.- Acta virol., 39, 235-241.
Doumanova, L., M. Alexandrov (1997): Detection and identification of the Newcastle
disease virus infection by electron and immunoelectron microscopy. Acta Virologica 41, 111114.
Doumanova, L., V. Neitchev (1998): Structural studies on the Newcastle disease virus
membrane protein by circular dichroism method. Anal. Lab. 7(4), 197-200.
Serkedjieva, J. A., A. Hay (1998): In vitro antiinfluenza virus activity of a plant
preparation from Geranium sanguineum L. - Antiviral Res., 1998, 37, 221-230.
Pancheva, S., I. Roeva, D. Dundarova, M. Remichkova (1999): Mycophenolic acid as
acyclovir partner for combined inhibition of herpesviruses. - Die Pharmazie, 54, 632-633.
Nikolaeva, L., A. S. Galabov (1999): Cytotoxicity of the synergistic antienteroviral
combination of enviroxime and disoxaril. – Acta virol., 43, 263-265.
Nikolaeva, L. A. S. Galabov (1999): In vitro inhibitory effects of dual combinations of
picornavirus replication inhibitors. – Acta virol., 43, 303-311.
Mileva, M., L. Tancheva, R. Bakalova, A. Galabov, V. Savov, St. Ribarov (2000):
Effect of vitamin E on lipid peroxydation and liver monooxygenase activity in experimental
influenza virus infection. –
Toxicology Lett. 114: 39-54.
Nikolaeva, L., A. S. Galabov (2000): Antiviral effect of the combination of enviroxim and
disoxaril on Coxsackievirus B1 infection. –
Acta virol. 44: 73-78.
Mileva, M., V. Hadjimitova, L. Tantcheva, T. Traykov, A. S. Galabov, V. Savov, St.
Ribarov (2000): Antioxidant properties of rimantadine in influenza virus infected mice and in
some model systems. – Z. Naturforsch. 55c: 824-829.
Mileva, M. R. Bakalova, L. Tancheva, A. Galabov, S. Ribarov (2002): Effect of vitamin
E supplementation on lipid peroxidation in blood and lung of influenza virus infected mice. –
Comparative Immun., Microbiol. & Infect. Dis., 25, 1-11.
Mileva, M., R. Bakalova, L. Tancheva, A. S. Galabov (2002): Effect of immobilization,
cold and cold-restraint stress on liver monooxygenase activity and lipid peroxidation of
influenza virus-infected mice. – Arch. Toxicol., 76, 96-103.
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Czegled A., J. Herczeg, G. Hadjiev, L. Doumanova, E. Wehnmann, B. Lomniczi
(2002): The occurrence of five major Newcastle disease virus genotypes ( II,IV, V, VI and
VIIb) in Bulgaria between 1959 and 1996. Epidemiol. Infect. 129, 1-10.
Tantcheva, L. P., E. S. Stoeva, V. M. Savov, M. M. Mileva, A. S. Galabov,
A. A. Braykova (2003): Effect of vitamin E and vitamin C combination on
experimental influenza virus infection. – Methods & Findings in Exper. & Clinical Pharmacol.,
25(4), 259-264.
Mileva, M., K. Marazova, A. S. Galabov (2003): Gastric mucosal lesions in influenza
virus infected mice: role of gastric lipid peroxidation. – Methods & Findings in Exper. Biol.
& Med., 25 (7), 521-524.
Nikolova, I., A. S. Galabov (2003): Development of resistance to disoxaril in Coxsackievirus B1
infected newborn mice. - Antiviral Res., 60, 35-40.
Galabov, A. S., B. Kamarinchev, D. Toncheva, T. Stoitsova, Tz. Dimitrov (2003):
Are viruses causative agents of the Balkan endemic nephropathy? – In: Proceed. Tenth Congress of
the Bulgarian Microbiologists, Plovdiv, October 9-12, 2002, Vol. I (A. S. Galabov and H. Najdenski,
eds.). Ed. Bulgurian Society for Microbiology & The Stephan Angeloff Institute of Microbiology,
BAS, Sofia, pp. 289-295.
Toncheva, D., A. S. Galabov, A. Laich, S. Atanassova, B. Kamarinchev, Tz. Dimitrov, D.
Fuchs (2003): Urinary neopterin concentrations in patients with Balkan endemic nephropathy
(BEN).- Kidney Intern., 64, 1817-1821.
Georgiev, D., P. Grosdanov, B. Slavchev, I. Karagyozov, A. S. Galabov, D. Toncheva (2003):
Frequencies of human papillomavirus types in Bulgarian women with cervical cancer. – Italian J.
Gynecology & Obstetrics, 15/3/4, 131-137.
Nikolaeva-Glomb, L., A. S. Galabov (2004): Synergistic combinations against the replication in
vitro of Coxsackievirus B1. –
Antiviral Res., 62, 9-19.
Raykov, Z., M. Aprahamian, A. Galabov, J. Rommelaere (2004): Oncolytic parvoviruses as
tools for cancer gene therapy. – Biotechnol. & Biotechnol. Eq,. 18/1, 3-9.
Mileva, M., R. Bakalova, G. Zlateva, A. S. Galabov, H. Ohba, M. Ishikawa, Y. Baba
(2005): Еffect of Rutin and Quercetin and the “Oxidative stress” induced by influenza virus
infection in mice alveolocytes.- In: Proceedings of the 2nd Symposium of the International
Society of Rare Sugars (Ed. K. Izumori), Kagawa University Press, Takamatsu, Japan, 2005,
pp.64-69.
Ivanova E., R. Toshkova, J. Serkedjieva (2005): A plant polyphenol-rich extract
restores the suppressed functions of phagocytes in influenza virus-infected mice. Microbes
and Infection, 7, 3, 391-398.
Pravchanska, R., P. Borissova, L. Doumanova, V. Neitchev, P. Laggner (2006):
Effects of NDV glycoproteins on the structural and thermal behaviour of 1,2-dihexadecyl-sn7
glycero-3-phosphatidylcholine lipid membranes under osmotic stress conditions. Colloids and
Surfaces B & Biointerfaces 53, 72-77.
Angelova L., M., Dalgalarrondo, I. Minkov, S. Danova, N. Kirilov, J.-M. Chobert, T.
Haertle, J. Serkedjieva, I. Ivanova (2006): A novel proteinaceous protease inhibitor - PISC2002 isolated from Streptomyces chromofuscus 34-1 with antiviral activity. Biochemica et
Biophysica Acta – General Subjects, 1760, 1210-1216.
Kramer, A., A. S. Galabov, S. A. Sattar, , L. Doehner, A. Pivert, C. Payan, M. H. Wolff, A.
Yilmaz, J. Steinmann (2006): Virucidal activity of a new hand disinfectant with reduced ethanol
content: comparison with other alcohol based formulations. – J. Hospital Infections 62/1, 98-106.
Galabov, A. S., A. Angelova (2006): Antiviral agents in the prevention and treatment of virusinduced diabetes.- Anti-Infective Agents in Medicinal Chemistr, 5, 293-307.
Remichkova, M,. N. Petrov, A. S. Galabov (2006): Synergistic combination effect of cidofovir
and idoxuridine on vaccinia virus replication.- Antiviral Chemistry and Chemotherapy, 17, 53-58.
Savov, V. M., A. S. Galabov, L. P. Tantcheva, M. M. Mileva, E. L. Pavlova, E. S. Stoeva, A.
A. Braykova (2006): Effects of rutin and quercetin on monooxygenase activities in experimental
influenza virus infection.- Experimental and Toxicologic Pathology, 58, 59-64.
Galabov, A. S., L. Simeonova, G. Gegova (2006): Rimantadine and oseltamivir demonstrate
synergistic combination effect in experimental infection with A(H3N2) influenza virus in mice.Antiviral Chemistry and Chemotherapy 17, 251-258.
Raykov, Z., S. Grekova, A. S. Galabov, G. Balboni, U. Koch, M. Aprahamian, J.
Rommelaere (2007): Combined oncolytic and vaccination activities of parvovirus H-1 in a
metastatic tumor model. - Oncology Reports, in press.
Nikolaeva-Glomb, L., S. Philipov, A. S. Galabov (2007): A new highly potent
antienteroviral compound. Proceedings 2006 NIAID Research Conference, Opatija, Croatia,
24-30.06.2006, in press.
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