From: Evidence of Dual Mechanisms of Glutathione Uptake in the Rodent Lens: A Novel Role for Vitreous Humor in Lens
Glutathione Homeostasis
Invest. Ophthalmol. Vis. Sci.. 2016;57(8):3914-3925. doi:10.1167/iovs.16-19592
Figure Legend:
Characterization of GSH transport in cultured lenses. All lenses were preincubated with 1 mM BSO and 500 μM acivicin
for 1 hour to prevent any breakdown or turnover of GSH-(glycine-13C2,15N). (A) Wild-type and LEGSKO lenses were
incubated with 500 μM GSH-(glycine-13C2,15N) at 37°C in 5% CO2 for 1 hour. No significant differences were observed
between uptake rates of LEGSKO and those of WT lenses. (B) Wild-type mouse lenses were incubated with 2 mM of
each substrate and taken at various time points. GSH-(glycine-13C2,15N) had the lowest initial uptake rate, followed by
15N -arginine and 13C -alanine (P < 0.005). (C) Lenses were incubated in various concentrations of substrates and taken
2
3
for analysis
within
the initial rate period
uptake. Curves
are best
fits of and
the data
to the Michaelis-Menten
equation.
Date
of download:
7/31/2017
TheofAssociation
for Research
in Vision
Ophthalmology
Copyright © 2017.
All rights reserved.
13
15
13
15
GSH-(glycine- C , N) showed a significantly lower rate of uptake than C -alanine and N -arginine at all