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UNIVERSITY OF PENNSYLVANIA
www.facebook.com/PennCellCenterServices
Location:
Tel:
Fax:
Website:
Mailing Address:
B14 Anat./Chem. Building
3620 Hamilton Walk
Philadelphia, PA 19104
B14 Anatomy/Chemistry Building
215/898-2795
215/573-9327
http://www.med.upenn.edu/cores/cell_center_services.shtml
REQUEST FOR LARGE SCALE CELL CULTURE AND PRODUCTION / PURIFICATION
OF ANTIBODY OR RECOMBINANT PROTEIN
User Name: __________________________________________ Penn Key_____________________________________
Principal Investigator: _________________________________ Fund No/ PO No: ______________________________
Date: _______________________________________________ Tel: __________________________________________
Email: ______________________________________________ Fax: _________________________________________
Prices subject to change. Contact Cell Center Services for current prices.
Name of cell line(s): _____________________________________________________________________
Protein produced: _________________________________ Size of antibody / protein: ____________ kD
Cell culture medium: _____________________________________________________________________
Please check the requested service(s)
Roller bottle culture: supernatant production: indicate desired volume ________________ L
Roller bottle culture: large scale production of cells: indicate total # of cells needed ______________ x 10 6
CelLine Flask 14/14
CelLine Flask 7/21
Antibody / recombinant protein production in a hollow fiber cartridge
Cartridge set-up:
medium
large
# of weeks: ________
Mab (IgG) purification using protein G column
Antibody conjugation
Protein purification (using other chromatography): Specify method ______________________________________
Antibody fragmentation: Fab fragments: :
Digestion
Purification
SDS-PAGE
Staff Use Only
Start Date _______________________
Revised 04062017
Completed ______________________
By: _________________________