AIBY meeting
21 May 2008
ICT, Prague – who we are?
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public university providing education and
pursuing scientific, research, development and
implementation activities,
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member of the family of 28 public and state
universities in the Czech Republic.
ICT, Prague
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Location
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Praha 6 - Dejvice
Technická 5
Czech Republic
www.vscht.cz
Structure
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Faculty of Chemical Technology
Faculty of Environmental Technology
Faculty of Food and Biochemical Technology
Faculty of Chemical Engineering
ICT, Prague - people
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Staff
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(780)
40 professors
100 associate professors
280 assistant professors
and assistants
Students (3100)
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2500 Master and Bachelor students
600 post-graduate students
Faculty of Food and Biochemical
Technology
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The Faculty of Food Technology was
established as part of the ICT, Prague in
1952.
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The faculty took the name Faculty of Food
and Biochemical Technology in the
academic year 1969-1970.
Faculty of Food and Biochemical
Technology
Department of
Fermentation
Chemistry and
Bioengineering
Department of
Dairy and Fat
Technology
Department of
Food Chemistry
and Analysis
Department of
Biochemistry and
Microbiology
Department of
Carbohydrate
Chemistry and
Technology
Department of Food
Preservation and
Meat Technology
Department of
Chemistry of Natural
Substances
Department of Fermentation
Chemistry and Bioengineering
Prof. Karel Melzoch
(Head of Department and Dean of Faculty)
Biotechnology Group
Brewery Group
Biotechnology group
• National and EU projects based on bioprocess
optimization, microbial physiology, downstream
processing, knowledge-based process control
• Training activities - biotechnology disciplines and
physiology
• Research activities – cultivations, recombinant
protein technology, bioprocess optimization,
physiology (flow cytometry), downstream
processing, analytics (HPLC, GC,…)
Biotechnology – teaching
programme
• Combination of the principles of biological,
chemical, engineering and economic studies
• role and behaviour of the biological element
(microbial, plant and tissue cells, fragments
and enzymes) regarding engineering and
technological aspects of the bioprocess and
connected up-stream and down-stream
processing
• application of computers in bioprocess
modelling, simulation studies and direct control
of bioreactors
• students participate in regular research work in
several research projects supported by Czech
and European Grant Agencies (diploma thesis,
PhD programme)
Biotechnology - research activities
• Optimization of bioprocesses
employing microbial cells
• Optimization of heterologous
protein production processes
• Study of physiological attributes
of cells population under
cultivation/stress condition
• Down-stream and Up-stream
processes
Cooperation with
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Great Britain: University of Birmingham, University of Surrey, Brunel University of
West London, Manchester Metropolitan University, University of Teeside
Middlesbrough
Portugal: INETI Lisboa, University of Lisbon
Switzerland – Zurich University of Applied Sciences
Poland: Akademia ekonomiczna, Wroclaw, Akademia rolnicza, Poznaň, Univesrsity of
Lodz, Dept. Indust. Microbiol.
Bulgary: Bulgarian Academy of Science, Inst. Microbiol., Sofia.
Russia: Moscow State Academy of Chemical Engineering
Holland: International Institute for Hydraulic and Environmental Engineering, Delft.
Hungary: Biological Research Centre, Hungarian Acad. Sci., Biotechnol. Inst., Bay Z.
Foundation Appl. Res. Techn. Szeged.
Austria: Technische Universität Graz.
Germany: Institut fü r Bioproceß und Analysenmeß technik Heiligenstadt,
Forsungszentrum für Medizintechnik und Biotechnologie, Bad Langensalza.
Italy: University of Naples "Federico II"
Spain: Universidad de Valencia
Projets
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TEMPUS-PHARE
COPERNICUS
COST
EUREKA
ERASMUS/SOCRATES
The Czech-American Program
projects supported by the Czech Grant Agency,
Czech Ministry of Agriculture National Agency for
Agricultural Research, the Czech Ministry of the
Environment, the Czech Academy of Sciences
Grant Agency, etc.
Our contribution to AIBY
Complete solution of heterologous protein
production processes employing bacterial
and yeast cells including cultivation,
process optimization, down-stream and
up-stream processes, process simulation,
analytics including the analysis of selected
physiological attributes of host strain
Cultivation of microorganisms
• Bacteria, yeasts, fungi
• B.Braun bioreactors (2 L, 5L, 7L)
• Possibility to scale-up to 1500 L
(cooperation with Institute of
Microbiology, Czech Academy of
Sciences – Biotechnological Pilot
Plant)
• 5-year experience with
recombinant cultures (Eureka
E!3415 succesfully finished in
2007)
Analysis of physiological attributes
of cell population
• Flow cytometry (Flow cytometers
Partec PAS III (Partec) and Quanta
(Beckman-Coulter)) Ar-Ion laser for
488 nm excitation and HBO lamp for
excitation in UVSSC, FSC, FL1(512545 nm), FL2(575-610 nm), FL3
(>630 nm)
• Analysis of cell population on single
cell level in rates of thousands in
very short time period
• Staining with fluorescent probes –
presence of active functions of cells
• Computer-aided picture analysis
LUCIA G (CR)
Probe
Physiological
parameter
PI, EB, Sytox Green
Viability, DNA
Acriflavine
Glycogen
FITC
Proteins
BOX, Rhodamine 123
Membrane potential
FDA, cFDA
Esterase activity
dead cells
viable cells
viable cells dead cells
Pichia pastoris stained with BOX
Pichia pastoris stained with PI
Cells stained with PI
Analytics
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HPLC analysis
GC analysis
Validation of methods
Maldi-Toff analysis and protein
sequencing
(in cooperation with
Department of Biochemistry
and Microbiology)
Mass spectrum of HPLC fraction determined
by MALDI-TOF (Pichia pastoris)
Down-stream proceses
• Electrodialysis
• Micro-, ultra and
nanofiltration
• Reverse osmosis
Membrane units with
ceramic membranes
Thank you for attention