Syllabus
MCB 301
Laboratory Schedule
Date (pt total)
Experiment
DAY 1
Course introduction
Mon Aug 24
1: Streak plate
Tu
2: Luminescent bacteria
10-13
DAY 2
1, 2: Evaluations
8, 13
Wed Aug 26
3: Spread plate
14-17
Th
4: Simple stain
18-29
DAY 3
3: Evaluations
17
Mon Aug 31
5: Gram stain
30-32
DAY 4 (12)
6: Wet mount, Darkfield and Phase Contrast
33-42
Wed Sept 2
7a-d: Enzymes and adaptation, 1
43-54
Tu
Aug 25
Aug 27
Lab Manual Pages
I-XXXII
1-9
Sept 1
Th Sept 3
Mon Sept 7
Tu
HOLIDAY - NO LABS
Sept 8
DAY 5 (33)
7a-d: Enzymes and adaptation, 2
43-54
Wed Sept 9
8a: Lactic acid bacteria enrichment
55-63
8b: Lactic acid bacteria isolation
63-64
DAY 7 (42)
8c: Lactic acid bacteria observation
64-65
Wed Sept 16
9a-b: Environmental effects, 1
66-75
DAY 8 (20)
9a-b: Environmental effects, 2
72-75
Mon Sept 21
9c-e: Environmental effects, 1
76-81
Th
Sept 10
DAY 6 (22)
Mon Sept 14
Tu Sept 15
Th
Tu
Sept 17
Sept 22
II
Syllabus
MCB 301
Date (pt total)
Experiment
DAY 9 (32)
9c-e: Environmental effects, 2
76-81
Wed Sept 23
10a: Sporeformers enrichment
82-93
10b: Sporeformers isolation, 1
94-95
10b: Sporeformers isolation, 2
96-97
Th
Lab Manual Pages
Sept 24
DAY 10 (12)
Mon Sept 28
Tu Sept 29
DAY 11 (13)
Wed Sept 30
Th
Oct 1
DAY 12 (13)
10c: Sporeformers post-enrichment, 1
98-99
Mon Oct 5
Tu
Oct 6
DAY 13 (20)
10c: Sporeformers post-enrichment, 2
Wed Oct 7
15: Antibiotic Producing Bacteria, 1
Th
99-102
Oct 8
DAY 14 (6)
15: Antibiotic Producing Bacteria, 2
Mon Oct 12
Tu
Oct 13
DAY 15 (29)
13a-c: Biochemical tests, 1
127-158
Wed Oct 14
14a: Identification isolation
161-168
DAY 16 (34)
13a-c: Biochemical tests, 2
127-159
Mon Oct 19
14b: Identification purification, 1
Tu
15: Antibiotic Producing Bacteria, 3
Th
Oct 15
Oct 20
Th Oct 20
OPEN HOURS BEGIN, SEE SCHEDULE
DAY 17 (13)
14b: Identification purification, 1
Wed Oct 21
15: Antibiotic Producing Bacteria, 4
Th
Oct 22
III
169
170
Syllabus
MCB 301
Date (pt total)
Experiment
Lab Manual Pages
DAY 18 (25)
14c: Identification dichotomous keys; inoculate
Mon Oct 26
tests, 1
Tu Oct 27
17a-b: MIC determination, 1
171
189-193
15: Antibiotic Producing Bacteria, 5
DAY 19 (24)
14c: Identification; inoculate tests, 2
Wed Oct 28
17a-b: MIC determination, 2
Th
15: Antibiotic Producing Bacteria, 6
Oct 29
DAY 20 (13)
14c: Identification; inoculate tests, 3
Mon Nov 2
15: Antibiotic Producing Bacteria, 7
172
194-195
172
Tu Nov 3
Tu Nov 3
OPEN HOURS END
DAY 21 (33)
12a: Transposon mutagenesis conjugation
114-118
Wed Nov 4
16a: ELISA fluid exchange
174-184
Th
Nov 5
DAY 22 (33)
12c: Transposon mutagenesis selection, 1
Mon Nov 9
16b: ELISA analysis
Tu Nov 10
15: Antibiotic Producing Bacteria, 8
DAY 23 (19)
12c: Transposon mutagenesis selection, 2
Wed Nov 11
15: Antibiotic Producing Bacteria, 9
Th
119
184-187
120
Nov 12
DAY 24 (12)
12c: Transposon mutagenesis characterization, 1
121-125
DAY 25 (19)
12c: Transposon mutagenesis characterization, 2
124-126
Wed Nov 18
15: Antibiotic Producing Bacteria, 10
Mon Nov 16
Tu Nov 17
Th
Nov 19
Sat Nov 21 to
Fall Break
Sun Nov 29
IV
Syllabus
MCB 301
Date (pt total)
Experiment
DAY 26
Lab Practical Review and Practice
Mon Nov 30
Lab clean up & drawer inventory
Tu
Dec 1
DAY 27 (42)
Wed Dec 2
Th
Dec 3
DAY 28 (58)
Mon Dec 7
Tu
Lab Manual Pages
Dec 8
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, brightfield
microscopy, streak plate
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase
contrast, biochemical tests, dichotomous key
V
Syllabus
MCB 301
Lecture Schedule
Date
Topic(s)
August 28
Lecture 1
Basic microbiology techniques (aseptic technique, pure culture, plating);
Luminescent bacteria; Microscopy; Staining (cell wall structure)
September 4
Lecture 2
Microscopy; Enzymes and adaptation
September 11
Lecture 3
Lactic acid bacteria
September 18
Exam 1
Basic microbiology techniques; Microscopy; Staining; Luminescent
bacteria; Enzymes and adaptation
September 25
Lecture 4
Bacterial Growth, Environmental effects
October 2
Lecture 5
Environmental effects; Sporeformers
October 9
October 16
Lecture 6
October 23
Lecture 7
Exam 2
Lactic acid bacteria; Bacterial growth; Environmental effects;
Biochemical tests; Identification; Antibiotic producing bacteria
DROP DEADLINE
Biochemical tests; MIC; Antibiotic producing bacteria
October 30
Lecture 8
ELISA/Agglutination; Antibiotic producing bacteria
November 6
Exam 3
Sporeformers; Identification; Biochemical tests
November 13
Transposon mutagenesis; Antibiotic producing bacteria
Lecture 9
November 20
Lecture 10
Antibiotic producing bacteria; Dilution problem
November 27
Fall Break
December 4
Lecture 11
Antibiotic producing bacteria
Final Exam
FINALS WEEK
Antibiotic producing bacteria; Transposon mutagenesis;
ELISA/Agglutination; MIC
VI
Syllabus
MCB 301
Laboratory Assignment Schedule
Date
Assignment Due
DAY 1
Mon Aug 24
Tu Aug 25
Nothing Due
DAY 2
Wed Aug 26
Th Aug 27
Nothing Due
DAY 3
Mon Aug 31
Tu Sept 1
Pre-lab 1-6: Basic Technique
10 points
DAY 4
Wed Sept 2
Th Sept 3
Pre-lab 7: Enzymes and adaptation
10 points
Mon Sept 7
HOLIDAY - NO LABS
Tu
Point Total
Sept 8
DAY 5
Wed Sept 9
Th Sept 10
Pre-lab 8: Lactic acid bacteria
10 points
Post-lab 1-6: Basic Technique
10 points
DAY 6
Mon Sept 14
Tu Sept 15
Post-lab 7: Enzymes and adaptation
10 points
DAY 7
Wed Sept 16
Th Sept 17
7: Enzymes & adaptation Lab Report
24 points
Pre-lab 9: Environmental effects
10 points
Fri Sept 18
Exam 1
Basic microbiology techniques; Microscopy;
Staining; Luminescent bacteria; Enzymes and
adaptation
50 points
DAY 8
Mon Sept 21
Tu Sept 22
Post-lab 8: Lactic acid bacteria
10 points
DAY 9
Wed Sept 23
Th Sept 24
Pre-lab 10: Sporeforming bacteria
10 points
8: Lactic acid bacteria Lab Report
65 points
Fri Sept 25
11: Growth Curve opens in LON-CAPA at 2pm
VII
Syllabus
MCB 301
Date
Assignment Due
DAY 10
Mon Sept 28
Tu Sept 29
Post-lab 9: Environmental effects
10 points
DAY 11
Wed Sept 30
Th Oct 1
9: Environmental effects lab report
60 points
DAY 12
Mon Oct 5
Tu Oct 6
Nothing Due
DAY 13
Wed Oct 7
Th Oct 8
11: Growth Curve exercise on LON-CAPA
41 points
Pre-lab 15: Antibiotic producing bacteria
10 points
Fri Oct 9
Point Total
Exam 2
Lactic acid bacteria; Bacterial growth;
Environmental effects;
50 points
DAY 14
Mon Oct 12
Tu Oct 13
Post-lab 10: Sporeforming bacteria
10 points
DAY 15
Wed Oct 14
Th Oct 15
10: Sporeforming bacteria Lab Report
63 points
Pre-lab 13: Biochemical Tests
10 points
Pre-lab 14: Identification
10 points
DAY 16
Mon Oct 19
Tu Oct 20
Nothing Due
DAY 17
Wed Oct 21
Th Oct 22
Post-lab 13: Biochemical tests
10 points
DAY 18
Mon Oct 26
Tu Oct 27
13: Biochemical Tests Lab Report
43 points
Pre-lab 17: MIC determination
10 points
DAY 19
Wed Oct 28
Th Oct 29
Nothing Due
DAY 20
Mon Nov 2
Tu Nov 3
Post-lab 17: MIC determination
VIII
10 points
Syllabus
MCB 301
Date
Assignment Due
DAY 21
Wed Nov 4
Th Nov 5
Pre-lab 12: Transposon mutagenesis
10 points
Pre-lab 16: ELISA
10 points
Post-lab: Identification
10 points
17: MIC Lab Report
24 points
Fri Nov 6
Point Total
Exam 3
50 points
Sporeformers; Identification; Biochemical Tests
DAY 22
Mon Nov 9
Tu Nov 10
14: Identification Lab Report
47 points
DAY 23
Wed Nov 11
Th Nov 12
Post-lab 16: ELISA
10 points
DAY 24
Mon Nov 16
Tu Nov 17
16: ELISA Lab Report
26 points
DAY 25
Wed Nov 18
Th Nov 19
Nothing Due
Sat Nov 21 to
Fall Break
Sun Nov 29
DAY 26
Mon Nov 30
Tu Dec 1
12: Transposon mutagenesis Lab Report (NO
LATE LAB REPORTS ACCEPTED)
15: Antibiotic producing bacteria (NO LATE LAB
REPORTS ACCEPTED)
Post-lab 12: Transposon mutagenesis
Post-lab 15: Antibiotic producing bacteria
DAY 27
Wed Dec 2
Th Dec 3
DAY 28
Mon Dec 7
Tu Dec 8
Finals Week
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain,
brightfield microscopy, streak plate
62 points
65 points
10 points
10 points
Parts 1 and 2 worth a
total of 100 points
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase
contrast, biochemical tests, dichotomous key
Final Exam
Antibiotic producing bacteria; Transposon
mutagenesis; ELISA/Agglutination; MIC
IX
50 points