B
A
30 µM ADP
0.25
mN
2 min
sub-urothelium
100 µM ADP
20 µm
urothelium
C
mN.min.g-1
9
6
3
detrusor
0
baseline 30 µM ADP
20 µm
D
10 µM PSB0474
1.0
mN
5 min
mN.min.g-1
20
15
10
5
0
baseline
10 µM
PSB0474
A
dome
baseline
base
30 µM UTP
B
[Ca2+]i
a.u.
1 mN
5s
5s
dome
baseline
base
30 µM ADP
Dome
baseline
Base
Dome
30 µM ADP
Base
Ca2+ maps
Em maps
Serosa
Detrusor Mucosa
baseline
Serosa
Detrusor Mucosa
30 µM UTP
Serosa
Detrusor Mucosa
30 µM UDP
A
B
0.44 s
baseline
0.24 s
30 µM ADP