Introduction to Cells Cells are the basic units of organisms Cells can only be observed under microscope Basic types of cells: Animal Cell Plant Cell Bacterial 1Cell Cells May be Prokaryotic or Eukaryotic Prokaryotes include bacteria & lack a nucleus or membrane-bound structures called organelles Eukaryotes include most other cells & have a nucleus and membrane-bound organelles (plants, fungi, & animals) copyright cmassengale 2 Prokaryotes • Most numerous organisms on Earth • Include all bacteria • Earliest fossils date 2.5 billion years old 3 Characteristics of Bacteria 4 Bacteria can have Different shapes 5 Bacillus - E. coli 6 Staphylococcus 7 8 Bacterial Structure • Microscopic prokaryotes • unicellular • No nucleus or membranebound organelles • Contain ribosomes – make proteins • Single, circular chromosome in nucleoid region - DNA 9 Bacterial Cell 10 Protection • Cell Wall made of Peptidoglycan • May have a sticky coating called the Capsule for attachment to host or other bacteria 11 Sticky Bacterial Capsule 12 Flagella • Bacteria that are motile have appendages called flagella • A bacteria can have one or many flagella 13 Monotrichous Lophotrichous Amphitrichous Peritrichous 14 Pili • Short protein appendages • Smaller than flagella • Adhere bacteria to surfaces • Used in conjugation for Exchange of genetic information 15 Pili in Conjugation 16 Bacteria Replication Binary fission Conjugation Use pilus 17 Bacteria • Most grow best at pH of 6.5 to 7.0 • Large portion of bacteria are useful • Only some cause disease 18 Useful Bacteria • Some bacteria can degrade oil • Used to clean up oil spills • Act as decomposers – nitrogen cycle 19 Useful Bacteria • Other uses for bacteria include making yogurt, cheese, and buttermilk. 20 21 22 23 • E. Coli = Gram Negative • Bacillus = Gram Positive 24 BIOFILM • The plaque that forms on teeth and causes tooth decay • Form when bacteria adhere to surfaces and secrete a slimy, gluelike substance • Forms communities of disease causing bacteria • Biofilms can form anywhere – clogged drains, slippery rocks ect.. 25 26 How Antibiotics Work • Interferes with cell wall – loses protections • Interferes with the bacteria’s ability to make proteins • Interferes with the ability to replicate DNA 27 Zone of inhibition 28 INQUIRY LAB • Test different types of mouthwash to determine which type kills the most bacteria. • Use the principles of antibiotic testing • Determine procedure – lab writeup – Purpose, hypothesis, materials, procedure, data, analysis, conclusion • Decide which two types of mouthwash you will test • Measurements – zone of inhibition • Come to a conclusion 29 30 31 32
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