設計嗜熱藍綠菌專用薄板反
應器以提高生質體產率及二
氧化碳固定速率
編號 : 101A12
朱逸鈴
李姿穎
闕凡瑜
林鈺婷
江允中
環境工程學系碩士班
環境工程學系學士班
環境工程學系碩士班
環境工程學系碩士班
環境工程學系碩士班
Introduction
• Utilization of Fossil fuels
- Excess emissions of CO2
• Excess emissions of CO2
- causes global warming and climate change
• Technologies for CO2 sequestration
- The process of carbon capture and storage
- Biological processes
- Physical processes
- Chemical processes
Advantage:
1.lower leakage possibility
2.lower financial costs
3.Sun light is the energy source
Objections:
1.Danger of leak
2.High financial costs
3.Energy requirements
2
Photosynthetic
organism
harvest
Light energy
CO2
Trees
VS.
Microalgae or cyanobacteria
Photosynthesis
Biomass
Areal CO2 fixation rate is much
higher
3
Introduction
Solubility of CO2 in water is low. Most
of the CO2 is easily leakage to the
atmosphere before microalgae
assimilation.
The culture medium in the
photobioreactor is easily acidified by
CO2.
4
Introduction
Alkaline solution
captures CO2
CO2
(15-20%)
CO2
(<10%)
CO2
(Residue)
Sunlignt
Solid/liquid
seperation
packing
P
P
NaHCO3
Alkaline
regeneration
The CO2 in the gas
phase is transferred to
bicarbonate and
carbonate in the liquid
phase.
Cyanobacteria can
utilize bicarbonate.
P
Alkaline
solution
Water Batch
Wat
Combustion
Alkaline
absorber
Culture
medium
er Bioenergy
Photobioreactor
Bath
Two stages of CO2 fixation:
chemo-absorption followed by cyanobacteria assimilation (HCO3-) (Hsueh et al., 2007)
Bottleneck: Microalgae or cyanobacteria should tolerant high alkalinity conditions
Novelty: Cultivating high alkalinity tolerance cyanobacteria
5
Introduction
• Microalgae culture systems
Flat plate photobioreactor： Low light path →Increase light availability
Bottlenecks:
1.Energy is accumulated in the flat plate photobioreactor easily.
2. Mesophilic microalgae can not tolerance high temperature.
3.The cooling system should be adopted to decrease the temperature in
the phptobioreactor for mesophilic microalgae cultivation.
Novelty:
1. Cultivating thermophilic cyanobacteria in the flat plate
photobioreactors to increase the light availability.
2. Design the flat plate photobioreactors for thermophilic
cyanobacteria.
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(Thermosynechococcus CL-1; TCL-1)
1. Fast growing
2. High HCO3- assimilation rate
3. Tolerant High alkalinity
4. Tolerant High temperature
High biomass productivity and
CO2 fixation rate
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High light
availability
High
biomass
productivity
No cooling
system
High CO2
fixation rate
8
140
Indoor cultivation
[1.5, 1000]
[1.5, 2000]
[3.0, 1000]
[3.0, 2000]
[light path of the photobioreactor, light intensity]
Biomass productivity (mg/L/h)
120
100
80
60
40
20
0
0
1
2
3
4
5
6
7
8
9
10
11
12
Biomass concentration (g/L)
300
[1.5, 1,000]
[1.5, 2,000]
[3.0, 1,000]
[3.0, 2,000]
280
260
240
CO2 fixation rate (mg/L/h)
220
200
180
160
140
120
100
80
60
40
20
0
0
1
2
3
4
5
6
7
8
9
10
11
Initial biomass concentration (g/L)
9
12
Light intensity Light intensity
2
(uE/m /s)
(lx)
250000
1800
55
Outdoor cultivation
Temperature
Atmosphere
1.84 g/L
3.85 g/L
4.57 g/L
50
1600
200000
1400
Light intensity
Horizontal (lx)
2
Vertival (uE/m /s)
45
Temperature ( C)
225000
175000
1200
150000
o
40
1000
125000
800
35
100000
600
75000
30
400
50000
25
20
0
9/17
22:30
5
10
15
9/18
3:30
9/18
8:30
9/18
15:30
9/18
17:30
25000
200
0
0
20
Time (hr)
55
6.0
1.38 g biomass/L/8h
5.5
50
5.0
4.5
45
o
Temperature ( C)
固碳效率：170 mg/L/h
每升藻液每8小時可固定
1.77 g二氧化碳
3.5
40
3.0
35
2.5
2.0
30
1.5
Temperature
Atmosphere
1.84 g/L
3.85 g/L
4.57 g/L
25
20
0
5
10
15
9/17
22:30
9/18
3:30
9/18
8:30
9/18
15:30
TCL-1 cell density 1.0
1.84 g/L
0.5
3.85 g/L
4.57 g/L
0.0
20
9/18
17:30
Time (hr)
10
Cell Density (g/L)
4.0
S.-H. Ho et al. / Biotechnology Advances 29 (2011) 189–198
Chlorella emersonii
Chlorella kessleri_02
Chlorella kessleri_01
Chlorella vulgaris_02
Chlorella vulgaris_01
Chlorella sp._05
Chlorella sp._04
Chlorella sp._03
Chlorella sp._02
Chlorella sp._01
Scenedesmus obliquus_03
Scenedesmus obliquus_02
Scenedesmus obliquus_01
Scenedesmus sp._02
Scenedesmus sp._01
Spirulina sp.
Euglena gracilis
Microcystis ichthyoblabe
Microcystis aeruginosa
Botryococcus braunii
Synechocystis aquatulis
Chlorococcum Littorale
Phaeodactylum tricornutum
Nannochloropsis sp.
Nannochloris sp.
The average value is about
400-600 mg L-1d-1
1.77g/L/d (8hr in winter)
0
200
400
600
800
1000 1200 1400 1600 1800 2000
CO2 fixation rate (mg L-1d-1)
11
Thanks for your attention
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