Relationships between early B- and NK-lineage lymphocyte
precursors in bone marrow
by Taku Kouro, Vinay Kumar, and Paul W. Kincade
Blood
Volume 100(10):3672-3680
November 15, 2002
©2002 by American Society of Hematology
Generation of immature NK cells in one-step, serum-free, stromal cell–free cultures.Lin− c-kitLo
cells were isolated from bone marrow as described in “Materials and methods” and placed in
serum-free, stromal cell–free cultures containing SCF, FL, IL-7, and ...
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
Most Lin− NK-cell progenitors are most abundant in the c-kitLo fraction of bone marrow
cells.Bone marrow cells bearing markers associated with various blood cell lineages were
rigorously depleted with magnetic beads and high-speed cell sorting (as described...
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
Both NK-and B-lineage lymphocytes derive from hormone-sensitive progenitors in the bone
marrow.Lin− c-kitLo bone marrow cells were prepared from mice implanted 7 days previously
with estrogen pellets (β-estradiol–treated mice) or from control animals (contr...
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
Most NK-cell precursors in the Lin−c-kitLo fraction are not directly sensitive to estrogen.Lin− ckitLo bone marrow cells were put into serum-free, stromal cell–free cultures along with vehicle
(control) or estrogen (β-estradiol).
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
The Lin− c-kitLoFlk-2+ fraction of bone marrow contains B/NK-bipotential progenitors as well as
committed B- and NK-cell progenitors.Single-cell cultures were initiated as described in
“Materials and methods.” Typical examples are shown of individual cultur...
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
B and NK lineages diverge and become independently regulated by IL-7 or IL-15.The Lin− c-kitLo
fraction of bone marrow cells was placed in primary cultures containing SCF, FL, and IL-7 for 5
days.
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
B-cell precursors are more enriched than NK-cell precursors among the IL-7Rα+ subset of
Lin−bone marrow cells.The Lin− c-kitLo Flk-2/Flt3+fraction of bone marrow was isolated and
further sorted into IL-7Rα+ and IL-7Rα− subsets before culture with SCF, FL, I...
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology
Restriction to the NK lineage occurs as Lin− c-kitLo cells acquire the CD122 receptor for IL15.Lin− c-kitLo cells were first cultured for 3 days with SCF, FL, and IL-7.
Taku Kouro et al. Blood 2002;100:3672-3680
©2002 by American Society of Hematology