17th APAN Meetings, University of Hawaii
2004.1.28-30
Laser-spectroscopic Information on
Agricultural Products as Future
Resources for Field server
Yasunori Saito*,
T. Koga*, T. Matsubara*, Y. Maruyama*, F. Kobayashi*,
A. Nomura*, H. Ishizawa** and K. Komatsu***
*Shinshu University (Engineering), Nagano, Japan
**Shinshu University (Textile Science & Technology), Ueda, Japan
***Nagano Vegetable and Ornamental Crops Experiment Station,
Nagano, Japan
Motivation
Future field server (next generation) will be required
more direct information on health and living status of
agricultural products.
Environment Information
Field Server
Temperature
Humidity
Sun Light
+
CO2
Water Content
Image
....
Direct Status Information
Physiological
Biological
Biophysical
Photobiological
Ecophysiological
…
Furture
Field Server
Purpose
Application of laser spectroscopy as a new method to
collect the direct status information of agricultural
products
Discussion on its possibility and usefulness
Methodology
Laser-induced Fluorescence Spectroscopy (LIFS)
LIFS for Agriculture Project
Laser
Fluorescence
Emission
Abnormal
Normal
Sample
Molecular
Energy Diagram
Spectrum
Vegetation Fluorescence as an Index for
Photosynthesis, Stress, Disease, Vitality, etc.
No-use Chemical Solution
Non Destructive Method
Remote Sensor
toward OPTICUL FARMING
Examples
1) Lettuce : Optimal Harvest Time Decision
2) Coffee : Leaves Vitality (?) Distribution Map
3) Tomato : Seedling’s Remote LIF Imaging
1) Lettuce : Optimal Harvest Time Decision
PC
Nd:YAG
Laser
Spectrometor
& CCD detector
Fluorescence
Detection
Fiber
Laser
Transmission
Fiber
Relative Intensity
Mobile LIF Monitoring System
2
LIF Spectra of Lettuce Leaf
1.5
Chlorophyll
Ferulic Acid
& Others
1
0.5
0
300
400
500
600
Wavelength (nm)
700
800
Outer Leaves
Relative Intensity
1.5
● ‘97
▲ ‘98
■ ‘99
1
0.5
Chlorogenic acid
Concentration (μg/g)
Harvest time
‘99 ‘98 ‘97
0
100
80
▲ ‘98
■ ‘99
60
■
■
40
■
20
■
■
0
0
10
20
30
40
Days after Planting
50
60
2） Coffee Tree Leaves : Vitality (?) Distribution Map
Image Intensified
Cooled CCD Camera
Liquid Crystal
Tunable Filter
CW Violet
Diode Laser
Controll Units
PC
Coffee Tree
LIF Imaging System
Relative Value to 660nm Fluorescence Image Intensity
5
0
460nm
530nm
Ferulic acid derivatives
Phenylpropanoids
NAD(P)H
685nm
740nm
Chlorophyll
Activated
？
3) Tomato : Seedling Remote LIF Imaging
Gated
ICCD
Camera
Liquid Crystal
Tunable Filter
0.3m
Camera
Controller
1.5m
PC
Fluorescence
Nd:YAG Laser
Tomato Seedling
15m
Remote Spectral Imaging of LIF
F740/F620
F720/F620
F685/F620
F660/F620
600
700
800
Wavelength (nm)
Conclusion
Proposal : LIF information for a future field server
System development and discussion of the usefulness
1) Lettuce : Optimal harvest time decision
2) Coffee tree leaves : Vitality distribution map
Needs direct comparison to
physiological data (evidence)
3) Tomato : Remote LIF imaging
More experimental discussion
required
Conclusion
Proposal : LIF information for a future field server
System development and discussion of the usefulness
1) Lettuce : Optimal harvest time decision
2) Coffee tree leaves : Vitality distribution map
Needs direct comparison to
physiological data (evidence)
3) Tomato : Remote LIF imaging
More experimental discussion
required
Plan
Field Server
Thank you very much !!
Mahalo Nui Loa !!
１）LIFスペクトル情報取得
ex 2） 形態付属情報の取得
実験方法
ハウス管理栽培トマト葉
移動型LIFスペクトル計測装置
Ａ６４-９　１ｓｈｏｔ
350
300
強度
250
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系列1
#9
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Ａ６４-７　１ｓｈｏｔ
50
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9 8
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#7系列1
50
0
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Ａ６４-４　１ｓｈｏｔ
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4
波長（ｎｍ）
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50
3
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#1
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-50200
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#2系列1
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A64-0
波長（ｎｍ）
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強度
500
強
400
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Ａ６４-２　１ｓｈｏｔ
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系列1
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#4系列1
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Ａ６４-１　１ｓｈｏｔ
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800 900
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5
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強度
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強度
強度
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-50200 300
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Ａ６４-５　１ｓｈｏｔ
Ａ６４-３　１ｓｈｏｔ
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#5系列1
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系列1
#3
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波長（ｎｍ）
Ａ６４-８　１ｓｈｏｔ
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強度
300
#8系列1
150
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波長（ｎｍ）
Ａ６４-６　１ｓｈｏｔ
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強度
-50 200
強度
強度
250
#6系列1
150
100
50
0
-50 200
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波長（ｎｍ）
700
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