AERATION-AGITATION
The role of oxygen , respiration, oxygen demand
Aeration agitation
MASS TRANSFER UNIT OPERATIONS
Scale up
Bioreaktor
ioreaktorss
Role of oxygen , respiration
RESPIRATION is an energy yielding metabolic process , in which an
organic or inorganic compound (energy source) is oxidized by the
organism with an inorganic compound.
If oxidizing agent is not oxygen, it is an
ANAEROBIC
ANAEROB
IC RESPIRATION
RESPIRATION
If it is oxygen, we call it
AEROBIC
AEROB
IC RESPIRATION
RESPIRATION
Role of oxygen, respiration
Energy source
(reducing=oxi-
Oxidant
(terminal elect-
dized compound)
ron acceptor)
H2
Products of
respiration
example
O2
H2O
Hydrogen bacteria
*H2
SO42-
H2O+S2-
Desulfovibrio
NH3
O2
NO2- + H2O
Nitrifiing bacteria
NO2-
O2
NO3-+H2O
Nitrifiing bacteria
NO3-
N2+CO2
Denitrifiing
Denitrifi
ing ba
bacteria
cteria
Fe2+
O2
Fe3+
Ferrobacillus
S2-
O2
SO2 + H2O
Thiobacillus
CO2+H2O
most of microorg.,plants
*organic cpd.
organic compound O2
and animals
Role of oxygen, respiration
Role of oxygen, respiration
(6 C-atom)
glükóz
ATP
ADP
G-6-P
F-6-P
ATP
ADP
F-1,6-diP
Gliceraldehid-P
1,3-diP-glicerát
ADP
(3C-atom)
2H
ATP
3-P-glicerát
2-P-glicerát
PEP
ADP
ATP
Pyr
Ac-CoA
CO2
Oxálacetát
2H
citrát
NAD
2H
Malát
Cis-akonitát
Fumarát
2H
CO2
Szukcinát
CO2
i-citrát
α− keto-glutarát
2H
koenzimQ
2*3 CO2 + 5*2H = C6 H12O6
2H
Role of oxygen, respiration
1/2O2
FP2
NAD
FP1
KoenzimQ
cyt b
2e-
cyt c
cyt a
cyt a3
2H+
KJ
0,4
NAD
H2O
ATP
ATP
ATP
0,27 V
51,2 kJ
168
Q
b
c
0,22 V
41,6 kJ
a
84
0,53 V
100 kJ
-0,8
a3
ADP + P i
ATP + H 2O
∆Gο=+7,3 kcal =30,7 kJ
Role of oxygen, respiration
OX
OXYGEN
YGEN DEMANDS OF MICROORGANISMS
Role of oxygen, respiration
Oxygen can be limiting substrate
Oxygen demand is expressed in two ways:
1. Respiration rate =
dc
dt
[ mmol O2/ dm3.h]],
[kg O2/ m3 .h]]
2. Specific respiration rate
1 dc
Q=
x dt
dx
c
= µ max
x
dt
K O 2 +c
[ h-1 ]
∆x
YO =
∆c
Role of oxygen, respiration
dc
1 dx
1
c
=−
=−
µ max
x
dt
YO dc
YO
K O2 + c
1 dc
1
c
Q=
=−
µ max
x dt
YO
K O2 + c
Q ≅ Q max
mO
1
1
= max +
YO YOG
µ
Q ≅ Q max
Oth order
Kinetics
First order
Kinetics
Q ≅ Q max
c
K O2
1 dc
c
Q=
= Q max
x dt
K O2 + c
Role of oxygen, respiration
µmax
- SPECIFIC GROWTH RATE
Yo - OVERALL YIELD COEFFICIENT FOR OXYGEN
mO – specific maintenance coefficient for oxygen
[g02/g cell.h
cell.h]]
max
YOG
-maximal yield for oxygen
Qmax – maximal specific oxy
oxygen demand or sp. resp. rate
KO2 - substrate saturation constant
Ccr – critical oxygen concentration
Specific resp rate
Glucose
Oxygen
Concentration in broth
1% ≈104 mg/dm3
7 mg/dm3
Critical concentration
50 mg/dm3
S U P P L Y
0,7 mg/dm3
580 mg/g.h
208 mg/g.h
Specific consumptio
rate
AERATION