Canal de cálcio tipo T e glioma neuroblastoma – mibefradil , bloqueador seletivo, aumenta
700-900% a ciclina1
Variation of T-type calcium channel protein expression affects
cell division of cultured tumor cells.
Panner A, Cribbs LL, Zainelli GM, Origitano TC, Singh S, Wurster RD.
Cell Calcium. 2005 Feb;37(2):105-19.
Source
Department of Neurological Surgery and The Brain Tumor Research Center, University of
California San Francisco, San Francisco, CA 94115, USA. [email protected]
Abstract
In this study we investigated the T-type calcium channel and its involvement in the cell division
of U87MG cultured glioma cells and N1E-115 neuroblastoma cells. Using Western blot analysis,
we found that expression of both alpha1G and alpha1H subunits of the T-type calcium channel
decreased during conditions associated with a decrease in proliferation as evidenced by
increased expression of cyclin D1, a marker for non-proliferating cells. Both serum starvation
and application of mibefradil, a selective T-type calcium channel antagonist, resulted in a 50%
decrease in the expression of alpha1G and alpha1H and a 700-900% increase in levels of
cyclin D1 in U87MG and N1E-115 cells, respectively. Furthermore, overexpression of the
alpha1H subunit resulted in a two-fold increase in cell proliferation compared to control cultures
or cultures receiving an empty vector. In contrast, blocking expression of the alpha1G subunit
using antisense oligonucleotides lead to a 70% decrease in proliferation of U87MG and N1E115 cells compared to control cultures or cultures receiving a scrambled oligonucleotide. Our
findings suggest that proliferation of U87MG glioma cells and N1E-115 is regulated by T-type
calcium channel expression.
Comment in
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Is there a role for T-type Ca2+ channel in glioma cell proliferation? [Cell Calcium. 2005]
PMID:15589991