Effects of Music on Microbial Substrate
Utilization of Aerobic Bacteria from
Municipal Wastewater Treatment Plant
PART I : Analytical Methods and Statistical Analysis
Suppajee Pornpongmetta1, Monthon Thanuttamavong2
Department of Environmental Engineering, Faculty of Engineering,
Kasetsart University, Bangkok 10900, Thailand
Abstract
Introduction
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Objectives
Materials and Methods
30
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Effects of Music on Microbial Substrate Utilization of
Aerobic Bacteria from Municipal Wastewater Treatment Plant
PART I : Analytical Methods and Statistical Analusis
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Study
Choices
Selected method
Reasons
Growth
- Enumeration
Turbidimetric
- Turbidimetric
(OD) with
- dry weight
dry weight
Easy, fast, Commonil used in a number of experiments, results
are comparable to the criteria
used to base design of wastewater
treatment plant.
- BOD
- COD
Substrate
- TOC
COD and TOC
utilization
- TN
- TP
The COD measures totall substrate utilization and is faster
to perform than BOD while TOC
measures organic carbon which
is a key component of microbial cells
The Best Faculty in Production of e and i Engineers
Effects of Music on Microbial Substrate Utilization of
Aerobic Bacteria from Municipal Wastewater Treatment Plant
PART I : Analytical Methods and Statistical Analusis
Method
Laboratory Apparatus
Washing and Sterilization
Preparation of Culture Media
Samples
Heterotrophic Plate Count
TOC
COD
Dry Weight
Standard
9030 B : Equipment Specifications
9040
9050 A : General Procedures
9050 B : Water
9060 B : Preservation and storage
9215 A : Introduction
9215C : Spread Plate method
Toc - V 5000 A analyzer (Sample were filtered
by Cellulose acetate membrane)
5220 C : Closed reflux method
2540 D : Suspended Solid (Sample were filtered by Cellulose acetate membrane)
S0(TOC)-S 24 (TOC)
t
x
S0(COD)-S 24 (COD)
t
x
= S0(TOC)-S 24 (TOC)
x 100
S0
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= S0(COD)-S 24 (COD)
x 100
S0
34
The Best Faculty in Production of e and i Engineers
Effects of Music on Microbial Substrate Utilization of
Aerobic Bacteria from Municipal Wastewater Treatment Plant
PART I : Analytical Methods and Statistical Analusis
Faculty of Engineering, Kasetsart University
35
Relevant equations for statistic calculation :
Percent confidence interval of the difference (%)
α=1100
(x - x )
t= 1 2
sp 1 + 1
n1 n2
Sp2 =
(n1 - 1) s12 ÷ (n2 - 1) s22
n1 + n2 - 2
P-value = P (t > |t|)
s12,s22
OD at 470 nm ( _ )
Results and Discussions
Figure 3 :Standard curve of OD-Dry weight cells
Dry weight (mg/L)
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Effects of Music on Microbial Substrate Utilization of
Aerobic Bacteria from Municipal Wastewater Treatment Plant
OD at 470 nm ( _ )
PART I : Analytical Methods and Statistical Analusis
Figure 4 :Standard curve of OD-TOC
OD at 470 nm ( _ )
TOC (mg/L)
Figure 5 :Standard curve of OD-COD
COD (mg/L)
Table 3 : Average parameters of control and treatment groups
Music
Group Dry weight at Dry weight at
0 hour (mg/L) 24 hour (mg/L)
Holy Wars…
Control
429.00
877.30
Treatment
429.00
884.96
Farewell
Control
426.00
839.50
Treatment
426.00
847.00
Oh la Nor…
Control
432.00
865.05
Treatment
432.00
893.63
“Ruk” music… Control
427.00
850.40
Treatment
427.00
882.04
Four seasons
Control
442.00
889.55
Treatment
442.00
947.32
Substrate at
0 hour
TOC
COD
(mg/L) (mg/L)
683.67 2168.00
683.67 2168.00
684.67 2171.00
684.67 2171.00
682.67 2165.00
682.67 2165.00
684.33 2170.00
684.33 2170.00
679.33 2155.00
679.33 2155.00
Substrate at
24 hours
TOC COD
(mg/L) (mg/L)
534.23 1720.00
531.68 1712.00
546.83 1758.00 544.33 1750.00
538.62 1732.00
528.79 1703.00
543.20 1747.00
532.65 1715.00
530.15 1707.00
510.89 1650.00
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Table 4 : Comparative Kinetics between control and treatment
Music
Group
Holy Wars…
Farewell
Oh la Nor…
“Ruk” music…
Four seasons
Control
Treatment
Increase
Control
Treatment
Increase Control
Treatment
Increase Control
Treatment
Increase Control
Treatment
Increase Substrate utilization
Specific substrate Efficiency
utilization rate
TOC
COD
TOC
COD
mg TOC removed
mg COD removed
(%)
(%) ( mg cells.hour ) ( mg cells.hour )
21.86
22.23
0.37
20.13
20.50
0.37
21.15
22.54
1.39
20.62
22.16
1.54
21.96
24.79
2.83
20.68
21.03
0.35
19.05
19.39
0.34
20.00
21.32
1.46
19.51
20.97
1.46
20.77
23.45
2.68
9.54x10-3
9.64x10-3
0.10x10-3
9.07x10-3
9.19x10-3
0.12x10-3
9.27x10-3
9.67x10-3
0.40x10-3
9.20x10-3
9.66x10-3
0.46x10-3
9.34x10-3
10.11x10-3
0.77x10-3
2.86x10-2
2.89x10-2
0.03x10-2
2.72x10-2
2.76x10-2
0.04x10-2
2.78x10-2
2.90x10-2
0.12x10-2
2.76x10-2
2.90x10-2
0.14x10-2
2.80x10-2
3.03x10-2
0.23x10-2
Table 5 : Statistical Analysis of the difference in Dry weight, TOC and COD between treatment and control groups before and after 24 hrs experimental period Music Percent Confidence Level of the Difference
between treatment and control groups (%)
Holy Wars The Punishment Due
Farewell Oh La Nor My love “Ruk” music of his Majesty King Bhumipol Adulyadej
The four seasons 38
DRY Weight
P-value
(_)
TOC
COD
85
85
99.5
99.5
85
85
99.5
99.5
85
85
99.5
99.5
0.131
0.102
0.000
0.000
99.5
99.5
99.5
0.000
The Best Faculty in Production of e and i Engineers
Effects of Music on Microbial Substrate Utilization of
Aerobic Bacteria from Municipal Wastewater Treatment Plant
PART I : Analytical Methods and Statistical Analusis
Conclusions
Acknowledgements
References
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