STARTING THE MICROSCOPE LOGON TO THE COMPUTER Using your logon and password. START SOFTWARE Start the four programs shown in the shortcut tray below, starting from left and to the right. 1. Microscope control 2. Filter control 3. Digital Micrograph 4. TIA (Technai imaging and analysis) Don’t start one program until the previous is up and running. (The software may start to argue about ownership of hardware) CHECK VACUUM Compare the Vacuum control panel from the “Microscope Control” software. The Gun vacuum MUST be 1 using Log scale. If not, something is wrong. Furthermore, the column must be less than 20 and is usually much lower. Finally the camera should be less than 30. OPEN COLUMN VALVE V7 Press the lower left button “Col. Valves Closed”. When the button is grey, the valve is open and electrons are able to travel down the optical axis. INCREASE THE EXTRACTION VOLTAGE The FEG extraction voltage should be set to a 3 kV during standby (to increase the FEG lifetime). Press the “Operate” button (turns yellow) to automatically increase the Extr. Voltage to 3800 V. By klicking the up/down arrows and the “Enter” button, you can increase the extraction voltage. 4000 is usually enough. There should now be a beam on the fluorescent screen.
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