Screening of anti-oxidative
effects in Camellia sinensis L.
leaves treated with boric acid
Işıl
a
İsmailoğlu
, Zeynep Mine
b
Coşkun ,
Melike
b
Ersöz ,
Murat Ali Turan
aDepartment
of Biology, Faculty of Arts and Sciences, Marmara University, Istanbul, Turkey
bDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Bilim University, Istanbul, Turkey
cDepartment of Soil Science and Plant Nutrition, Faculty of Agriculture, Uludag University, Bursa, Turkey
INTRODUCTION
Camellia sinensis L. (tea) is one of the most widely consumed drink in the World. The anti-oxidant role of boric acid has been
reported. The present study was aimed to evaluate the alteration of anti-oxidative effects of C. sinensis L. leaves extract treated
with boric acid.
MATERIAL AND METHOD
RESULTS
C. sinensis was grown up in Rize, Turkey. The land was divided
MDA level in tea leaves showed a significant alteration among
into four group. Each group was occured five areas (10 m2). The
four groups at first period. At second period, it was seen that
first group is control. Boric acid in concentration range of 100,
MDA level increased at 100 mg/m2 concentration of boric acid
300, 500 mg/m2 in sodium tetraborate buffer were applied as a
although MDA level reduced at 300, 500 mg/m2 concentration of
single dose on the second, third and fourth groups,
boric acid. There was a significant change in GSH levels among
respectively in March 2013. C. sinensis leaves were collected on
all groups at first period. A difference did not determined in
two different periods (May and July 2013). The levels of
SOD levels among 100, 300, 500 mg/m2 concentration of boric
malondialdehyde (MDA) and reduced glutathione (GSH), the
acid at first period. However, CAT levels elevated at 500 mg/m2
activities of superoxide dismutase (SOD) and catalase (CAT)
concentration of boric acid at first period (Table 1).
were measured in C. sinensis leave samples.
Table 1: Biochemical parameters in all groups
First Period
MDA*
Control
100
300
1.72 ± 0.31
0.87±0.06a
0.69 ± 0.10a
Second Period
500
PANOVA
Control
0.76 ± 0.09a P˂0.05 1.23 ± 0.08
100
300
500
PANOVA
1.55 ± 0.14
1.09 ± 0.10c
1.38 ± 0.12
NS
(nmol/mg)
GSH*
66.19 ± 11.64 27.02 ± 9.20b 18.73 ± 2.79a 14.93 ± 1.96a P˂0.05 57.43 ± 8.04 48.78 ± 3.71 38.26 ± 2.58b,c 51.45 ± 6.60
(nmol/mg)
SOD*
3.51 ± 0.93 1.60 ± 0.21b 1.31 ± 0.24b 1.41 ± 0.15b
NS
1.41 ± 0.19 1.99 ± 0.35
1.50 ± 0.13
1.78 ± 0.30
(U/mg)
CAT*
4.21 ± 1.08
4.40 ± 0.55 4.65 ± 0.70 6.12 ± 0.19c
NS
5.62 ± 1.51 3.32 ± 0.28
3.96 ± 1.11 4.84 ± 0.44d
(U/mg)
*Mean ± Standart Error of Mean (SEM), NS: Non-significant
aP<0.01 versus control; bP<0.05 versus control; cP<0.05 versus 100 mg/m2; dP<0.01 versus 100 mg/m2
CONCLUSION
In conclusion, it is suggested that treated boric acid may be elevated antioxidant status of C. sinensis at second collection period.
ACKNOWLEDGEMENT: We thank to Research Foundation of Marmara University (Number FEN-DKR-250405-0114) for financial support.
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