Supplementary Table ST3: Result of BLASTN of SSU rRNA test dataset derived from 43 species against SSUrdb (see right column for species name).
SSUrdb
Cellular
organisms
10/8600
Domain
Bacteria
6390/6400
99.8%
Color coding:
100%
<100%-95%
<95%-90%
<90%-80%
<80%-70%
<70%
Phylum
Proteobacteria
2670/2800
94.5%
Spirochaetes 197/200 = 98.5%
Cyanobacteria 194/200 = 97%
Firmicutes 595/600 = 99.2%
Aquificae 191/200 = 95.5%
Chlamydiae 198/200 = 99%
Bacteroidetes 396/400 = 99%
Chlorobi 197/200 = 98.5
Chloroflexi 181/200 = 91.5%
Deinococcus-Thermus 96%
Planctomycetes
391/400 = 97.8%
Actinobacteria 400/400= 100%
Archaea
1000/1000
100%
Eukaryota
1198/1200
99.8%
Acidobacteria 200/200 =100%
Thermotogae 199/200= 99.5%
Euryarchaeota 400/400=100%
Crenarchaeota 600/600=100%
Viridiplantae 372/400 = 93%
Fungi 193/200 = 96.5%
Alveolata 196/200 = 98%
Metazoa 400/400 = 100%
Class
Alpha 390/400 = 96.8%
Order
Rhizobiales 287/400 = 71.8%
Family
Bradyrhizobiaceae
Beta 550/600 = 91.6%
Burkholderiales 187/200 = 93.5%
Nitrosomonadales 313/400 = 78.3%
Burkholderiaceae
Nitrosomonadaceae
Gamma 1212/1400 = 86.6%
Enterobacteriales 584/800 = 73%%
Enterobacteriaceae
Pseudomonadales 299/400 = 75%
Pseudomonadaceae
Moraxacellaceae
Chromatiaceae
Myxococcaceae
Helicobacteraceae
Spirochaetaceae
Nostocaceae
Bacillaceae
Clostridiaceae
Mycoplasmataceae
Aquificaceae
Parachlamydiaceae
Bacteroidaceae
Flavobacteriaceae
Chlorobiaceae
Delta 200/200 = 100%
Epsilon 187/200 = 93.5%
Spirochaetes 197/200 = 98.5%
Bacilli
Clostridia
Mollicutes
Aquificae
Chlamydiae
Bacteroidetes
Flavobacteria
Chlorobia
Dehalococcidetes 166/200=83%
Deinococci
Planctomycetacia
Actinobacteria
Solibacteres
Thermotogae
Methanomicrobia 171/200=85.5
Halobacteria 200/200 = 100%
Thermoprotei 200/200 = 100%
GroupI.1a 192/200 = 96%
GroupI.1b 176/200 = 88%
Spermatophyta 325/400=81.3%
Ascomycota 173/200 = 86.5%
Apicomplexa 196/200 = 98%
Arthropoda 195/200 = 97.5%
Nematoda 200/200 = 100%
Chromatiales 161/200 = 80.5%
Myxococcales 191/200 = 95.5%
Campylobacterales 187/200=93.5%
Spirochaetales 197/200 = 98.5%
Nostocales 75/200 = 37.5%
Bacillales 192/200 = 96%
Clostridiales 194/200 = 97%
Mycoplasmatales 200/200 = 100%
Aquificales 191/200 = 95.5%
Chlamydiales 178/200 = 89%
Bacteroidales 196/200 = 98%
Flavobacteriales 42/200 = 21%
Chlorobiales 66/200 = 33%
Deinococcales 191/200 = 96%
Planctomycetales
391/400 = 97.8%
Actinomycetales 377/400 = 94.3%
Solibacterales 24/200 = 12%
Thermotogales 199/200 = 99.5%
Methanosarcinales170/200
Halobacteriales 200/200 = 100%
Sulfolobales 199/200 = 99.5%
Poales 30/200 = 15%
Brassicales 43/200 = 21.5%
Saccharomycetales 123/200=61.5
Haemosporida 196/200 = 98%
Diptera 192/200 = 96%
Rhabditida 120/200 = 60%
Deinococcaceae
Planctomycetaceae
Unclass. Planct.
Mycobacteriaceae
Streptomycetaceae
Solibacteraceae
Thermotogaceae
Methanosarcinaceae
Halobacteriaceae
Sulfolobaceae
Poaceae
Brassicaceae
Saccharomycetaceae
Drosophilidae
Rhabditidea
Genus
Bradyrhizobium
Nitrobacter
Burkholderia
Nitrosomonas
Nitrosospira
Salmonella
Shigella
Yersinia
Escherichia
Pseudomonas
Acinetobacter
Nitrosococcus
Myxococcus
Wolinella
Borrelia
Anabaena
Bacillus
Clostridium
Mycoplasma
Aquifex
Cand. Protochlamydia
Bacteroides
Flavobacterium
Chlorobaculum
Dehalococcoides
Deinococcus
Rhodopirellula
Candidatus Kuenenia
Mycobacterium
Streptomyces
Solibacter
Thermotoga
Methanosarcina
Haloquadratum
Sulfolobus
Cenarchaeum
Oryza
Arabidopsis
Saccharomyces
Plasmodium
Drosophila
Cenorhabditis
Species
B. japonicum
N. winogradskyi
B. cepaia
N. eutropha
N. multiformis
S. typhimurium
S. dysenteriae
Y. pestis
E. coli K12
P. fluorescens
A. baumannii
N. oceani
M. xanthus
W.succinogenes
B. burgdorferi
A. variabilis
B. anthracis
C. tetani
M. genitalum
A. aeolicus
P. amoebophila
B. thetaiomicron
F. johnsoniae
Chl. tepidum
D. sp. CBDB1
D. geothermalis
R. baltica
K. stuttgartiensis
M. leprae
S. coelicolor
S. usitatus
T. maritima
M. mazei
H. walsbyi
S. solfataricus
C. symbiosum
Unc. Cren. 54d9
O. sativa
A. thaliana
S. cerevisiae
P. falciparum
D. melanogaster
C. elegans
The data set consisted of 8,600 simulated ribo-tags of 100bp length (200 randomly generated ones from each species). The ribo-tags were taxonomically
affiliated according to a BLASTN bit score of 86, and BLASTN hits within the top ten percent of Bit score were included in the taxonomic analysis with MEGAN.
The number and percentage of ribo-tags for each species are given at different taxonomic resolution, from the domain until the order level. The color coding
refers to different percentages of ribo-tags correctly affiliated at a given taxonomic level. No ribo-tag was falsely assigned. 10 ribo-tags were affiliated as "cellular
organisms", i.e. could not be assigned to respective domain of live.