MasterPure™ Plant Leaf DNA Extraction Solution
Purification of plant-leaf DNA for microsatellite typing,
PCR amplification, restriction digestion, Southern blotting,
and other molecular biology applications.
The MasterPure™ Plant Leaf DNA Purification Kit is
designed for purifying DNA from 35-100 mg of fresh plantleaf tissue. The protocol can be completed in less than 1
hour and may be scaled up to obtain larger amounts of
DNA. The purified DNA can be used for PCR
amplification, restriction digestion, Southern blotting, or
other molecular biology applications.
The MasterPure Kit was designed for even the most
difficult plant leaf DNA purifications. The kit was optimized
with the grapevine, Vitis vinifera, whose leaves are
notoriously high in polyphenolic (tannin-like) compounds
and polysaccharides, which can interfere with both the
isolation and subsequent use of DNA. Plants tested
include grape, apple, tomato, maize, sugarcane,
sunflower, fern, quillwort, and pine.
FailSafe™PCR System
How does it work?
Benefits
•Recover DNA from the leaves of many plant species, even those high
in polysaccharides and polyphenolic compounds.
•Purify DNA in less than 1 hour.
•Yields high-molecular-weight DNA (Fig. 1).
•No organic solvent or CTAB extractions, CsCl gradients, or enzyme
digestions.
•Recover DNA of higher integrity than column-based purification
methods
The patented FailSafe ™ PCR Premix Selection Kit contains 12 FailSafe PCR Premixes to cover a
meticulously determined matrix of PCR conditions that give optimal PCR results for different sequences. Each
Premix represents a unique PCR condition, with everything needed for successful PCR. Combine the template
and primers with the FailSafe Enzyme Mix, add one volume of this cocktail to one volume of each of the 12
FailSafe PCR 2X Premixes, and cycle. We guarantee that at least one FailSafe PCR PreMix will provide
conditions that are optimal for the template and primers tested.
Masterpure™ Plant Leaf DNA Extraction Solution
MPP92010
10 purifications
£38-00
MPP92100
100 Purifications
£160-00
FailSafe™ PCR PreMix Selection Kit
(all 12 Premixes)
FS99060
60 Units
£96-00
Quote “FAIL15” When
you order the
Masterpure™ Plant leaf
DNA extraction solution
and the Failsafe™ PCR
system together to
receive your 15%
discount.