McAbee et al. Online Resources 1 2 3 4 5 6 7 8 Online Resource 1. Variation in soil temperature at 0-15-cm depths between May 15 and September 12, 2014 in A. cristatum monoculture plots (panel A) and native sagebrush steppe vegetation plots (panel B). Data collection coincided with Rsoil measurements, and symbols -June are missing due to equipment failure. 9 10 11 12 13 1 14 15 16 Time Interval Vegetation Campaign Date 1-Jul Overall F 12.22 Overall P 0.0077 AMB > SUM 0.0062 Native 29-Jul 25.49 0.0012 AMB > SUM 0.0010 WIN > SUM 0.0133 4-Jun 12.45 0.0073 AMB > WIN 0.0095 SUM > WIN 0.0152 12-Aug 20.61 0.0177 AMB > SUM 0.0164 5.5 0.0440 WIN > SUM All two-way P > 0.05 0.0488 1-May 15-May 5.71 0.0409 WIN > SUM 0.0365 18-Jun 98.53 0.0018 WIN > AMB 0.002 WIN > SUM 0.0033 29-Jul 15.64 0.0042 SUM > AMB 0.0049 SUM > WIN 0.0109 12-Aug 5.94 0.0378 SUM > AMB 0.0442 15-May 6.41 0.0324 WIN > SUM 0.0335 4-Jun 31.05 0.0007 WIN > SUM 0.0011 WIN > AMB 0.0013 18-Jun 33.96 0.0087 WIN > SUM 0.0096 WIN > AMB 0.0149 1-Jul 8.73 0.0167 WIN > AMB 0.0170 WIN > SUM 0.0490 Dawn CWG Native Mid-Day CWG Evening CWG Post-Hoc Comparison Tukey's P 29-Jul 6.16 0.0351 SUM > AMB 0.0322 12-Sep 5.62 0.0422 SUM > WIN 0.0395 17 18 19 20 21 22 Online Resource 2. Summary ANOVA table for the effects of irrigation (ambient, +summer, +winter) on NEE within each campaign date and within vegetation treatment (A. cristatum and native sagebrush steppe), with post-hoc Tukey comparisons. Only dates at which an overall effect (P < 0.05) was detected are recorded. 23 24 25 26 2 27 28 29 30 31 32 33 34 35 36 37 38 Online Resource 3. Dawn, mid-day, and evening Anet of the three most abundant plant species (A. cristatum, H. boreale, and A. tridentata) averaged across the growing season. Letters indicate which species groups are significantly different from others (P < 0.05) within a single diurnal interval. Error bars represent one standard error of the mean. Because Anet means did not vary significantly across the growing season, or between vegetation and irrigation treatments, these data represent means across those variables. Here, greater dark respiration (Anet measured at dawn interval) is indicated by a more negative Anet. 39 40 41 42 43 44 45 46 3 47 48 49 50 Online Resource 4. Seasonal variation in Anet of the three most abundant plant species in native vegetation plots (A. cristatum, a-c; H. boreale, d-f; A. tridentata, g-i). 51 4 52 53 54 55 56 57 58 59 60 61 62 Online Resource 5. Variation in leaf-level Anet with soil volumetric water content at dawn (top panels) and midday (bottom panels) among ambient, summer, and winter plots. Soil moisture data are averaged across 0.2-1.0 m depths. Panels A and B present Anet averaged across all plant species available at the time of each measurement campaign. Panels C and D present Anet for A. tridentata shrubs only (the dominant plant in each plot). Because moisture sensors were located only in native vegetation plots, Anet data are presented only for native vegetation plots (no data presented for A. cristatum monoculture plots). No regression lines are displayed because R2 values were less than 0.31 and P values were greater than 0.21 for all fits. 63 64 65 66 5 67 68 Time Interval Vegetation Native Campaign Date 1-Jul Overall F 11.71 Overall P 0.0085 SUM > AMB 0.0277 SUM > WIN 0.0017 SUM > AMB 0.0004 WIN > SUM 0.0021 WIN > AMB 0.0089 4-Jun 20.31 0.0021 1-Jul 8.61 0.0173 WIN > AMB 0.0152 15-Jul 9.16 0.0150 SUM > AMB 0.0123 29-Jul 9.90 0.0126 SUM > WIN 0.0448 SUM > AMB 0.0121 WIN > SUM 0.0364 WIN > AMB 0.0087 SUM > WIN 0.0015 SUM > AMB 0.0002 SUM > WIN 0.0118 SUM > AMB 0.0039 SUM > WIN 0.0019 SUM > AMB 0.0023 SUM > WIN 0.0006 SUM > AMB 0.0002 SUM > WIN 0.0027 SUM > AMB 0.0006 Native 29-Jul 15-Jul CWG 29-Jul Native 29-Jul CWG 0.0086 0.0004 15-Jul Evening *† WIN > AMB 39.27 4-Jun Mid-Day Tukey's P 29-Jul Dawn * CWG Post-Hoc Comparison 11.33 44.46 16.51 34.14 52.08 32.09 0.0092 0.0003 0.0036 0.0012 0.0002 0.0006 69 * Excludes data from campaign dates specified in Table 4 footnotes. 70 † SUM Rsoil marginally greater than AMB Rsoil in Native (P = 0.0543) and CWG plots (P = 0.0513) in July 71 72 73 74 75 Online Resource 6. Summary ANOVA table of the effects of irrigation treatments (ambient, +summer, +winter) on across-species Rsoil within each campaign date and within vegetation treatment (A. cristatum and native sagebrush steppe), with post-hoc Tukey comparisons. Only dates at which an overall effect (P < 0.05) was detected are recorded. 76 77 78 6
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