Version: 1
Revision Date: 11/30/2012
PE anti-human CD55 Antibody
Catalog# / Size
311308 / 100 tests
Clone
JS11 (See other available formats)
Other Names
DAF (Decay Accelerating Factor)
Isotype
Mouse IgG1, κ
Ave. Rating
★★★★★ 0 reviews
Description
CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain
glycoprotein also known as decay-accelerating factor (DAF). It is expressed
on hematopoietic cells including erythrocytes and many non-hematopoietic
cells. CD55 accelerates the dissociation of the components of the C3convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase
of the classical pathway, and factor Bb from the C3bBb complex, a C3convertase of the alternative pathway) to protect cells from inappropriate
damage caused by autologous complement. CD55 has been reported to
reduce the efficiency of NK cell lysis and induce signal transduction in T
cells. CD55 has also been shown to interact with CD97 and bind to
Coxsackie and Echovirus.
Reactivity
Human
Human peripheral blood lymphocytes stained
with JS11 PE, then detected with anti-mouse
IgG FITC
See PE spectral data
View Moxi Flow Data
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
(origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal
conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our
Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please
check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl
staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for
optimal performance for each application. Read more at www.biolegend.com/testsize regarding
the test size change.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
2. Peyron P, et al. 2000. J. Immunol. 165:5186
3. DeZern AE, et al. 2014. Exp Hematol. 42:857. PubMed
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_314865 (BioLegend Cat. No. 311308)
Antigen Details
Structure
GPI-achored regulator of complement activation (RCA), type I membrane glycoprotein, 60-70
kD
Distribution
Hematopoietic cells including erythrocytes and many non-hematopoietic cells
Function
Protects against inappropriate complement activation and cell damage, induces signal
transduction, reduces the efficiency of NK cell lysis
Ligand Receptor
C3b, C4b, CD97, Coxsackie viruses
Antigen References
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Hamann J, et al. 1996. J. Exp. Med. 184:1185.
3. Fujita T, et al. 1987. J. Exp. Med. 166:1221
Workshop
VI N-L060
Gene ID
1604
UniProt
View information about CD55 on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Cell Surface Immunofluorescence Staining Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
NeuroscienceProducts > Neuroinflammation > Neuroinflammation Antibodies >
Immunology > Human Immunology > Stem Cells > CD55 (DAF) >
Immunology > Human Immunology > CD and Related Molecules > CD55 (Decay Accelerating Factor) > JS-11 >
Other Formats
Description
Clone
Applications
APC anti-human CD55
JS11
FC
Biotin anti-human CD55
JS11
FC
FITC anti-human CD55
JS11
FC
Purified anti-human CD55
JS11
FC
PE/Cy7 anti-human CD55
JS11
FC
PerCP/Cy5.5 anti-human CD55
JS11
FC
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or
other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or
used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties
without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited
Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or
animal diagnostic, therapeutic or commercial use.
BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587