J. Microbiol. Biotechnol. (2013), 23(11), 1–3
http://dx.doi.org/10.4014/jmb.1607.07025
jmb
Fig. S1. Component analysis of Enteromorpha prolifera treatment with community DZ21.
Untreated: untreated E. prolifera, D3 and D7: treatment with community DZ21 for 3 and 7 days, respectively. CF crude fiber, TS total soluble
sugars, TDS total dissolved solids, RS reducing sugars.
Fig. S2. The electrophoresis result of community DZ21 DNA preparation by SDS-CTAB method.
M, λ-Hind III digest DNA Marker; L1, L2 refer to the duplicating work, respectively.
2
Name et al.
Table S1. Homologous genes classified in glycoside hydrolase (GH) families from DZ21.
EC number
GH family
Enzymes
3.2.1.21
GH1
beta-glucosidase
3.2.1.8
GH10
endo-1,4-beta-xylanase
2.4.1.18
GH13
1,4-alpha-glucan branching enzyme
3.2.1.1
GH13
alpha amylase
3.2.1.20
GH13
alpha-glucosidase
3.2.1.10
GH13
oligo-1,6-glucosidase
3.2.1.41
GH13
pullulanase
5.4.99.16
GH13
maltose alpha-D-glucosyltransferase
3.2.1.22
GH36
alpha-galactosidase
3.2.1.23
GH42
beta-galactosidase
3.2.1.55
GH51
alpha-L-arabinofuranosidase
3.2.1.55
GH51
alpha-N-arabinofuranosidase
3.2.1.37
GH52
xylan 1,4-beta-xylosidase
3.2.1.89
GH53
endo-beta-1,4-galactanase
2.4.1.18
GH57
glycogen branching enzyme
3.2.1.139
GH67
alpha-glucuronidase
2.4.1.25
GH77
alpha-glucanotransferase
3.2.1.4
GH9
cellulase
3.2.1.141
GH13
maltooligosyltrehalose trehalohydrolase
3.2.1.14
GH9
chitinase
3.2.1.40
GH78
alpha-L-rhamnosidase
3.2.1.4
GH45
endoglucanase
J. Microbiol. Biotechnol.
Title
3
Table S2. Enzymes detected in DZ21 involved in hydrolysis of cellulosic materials.
Contigs
name
Number
of reads
E
value
Identity
Genbank id
Enzyme name [species name]
EC
GH
family
KO
number
contig10396
1
1e-93
72
ref|WP_015844585.1|
beta-glucuronidase [Paenibacillus sp. JDR-2]
3.2.1.23
GH42
K01195
contig9773
1
1e-88
82
emb|CDP52142.1|
beta-xylosidase [Devosia sp. DBB001]
3.2.1.37
GH52
K01198
contig16614
4
1e-119
71
ref|WP_008830707.1|
xylan 1,4-beta-xylosidase [Sphingomonas sp. LH128]
3.2.1.37
K01198
contig17081
24
1e-159
99
ref|WP_013836349.1|
xylan 1,4-beta-xylosidase [Novosphingobium sp. PP1Y]
3.2.1.37
K01198
contig17068
27
0.0
73
ref|WP_026091392.1|
xylan 1,4-beta-xylosidase [Blastomonas sp. AAP53]
3.2.1.37
GH43
K01198
contig16666
5
0.0
83
ref|WP_028971025.1|
alpha-N-arabinofuranosidase [Sphingopyxis baekryungensis]
3.2.1.55
GH51
K01198
contig17081
24
1e-140
99
ref|WP_013836351.1|
alpha-N-arabinofuranosidase [Novosphingobium sp. PP1Y]
3.2.1.55
contig16918
16
4e-27
98
ref|WP_013836360.1|
alpha-L-arabinofuranosidase [Novosphingobium sp. PP1Y]
3.2.1.55
K01209
contig12815
1
2e-56
68
gb|KHF36566.1|
intracellular exo-alpha-L-arabinofuranosidase 2 [Paenibacillus sp. P1XP2]
3.2.1.55
K01209
contig5665
5
1e-114
65%
ref|WP_024462109.1|
pectin esterase [Marinimicrobium sp. LS-A18]
3.1.1.11
K01051
contig7072
2
2e-17
55%
ref|WP_033020944.1|
pectate lyase [Pseudoalteromonas haloplanktis]
3.1.1.11
K01051
K01209
contig11778
1
4e-28
54%
ref|WP_031231638.1|
pectate lyase [Asticcacaulis sp. YBE204]
3.1.1.11
contig2786
2
6e-07
72%
ref|WP_025158040.1|
endoglucanase [Leifsonia aquatica]
3.2.1.4
K01051
contig4069
1
4e-58
69%
gb|KGK84077.1|
cellulase [Pseudomonas sp. HMP271]
3.2.1.4
K01179
contig4673
11
6e-84
94%
ref|WP_003043556.1|
cellulase [Sphingopyxis sp. MC1]
3.2.1.4
K01179
contig4900
3
3e-69
81%
gb|EXF44478.1|
endoglucanase [Pseudomonas sp. BAY1663]
3.2.1.4
K01179
contig5764
3
7e-15
59%
emb|CDF96201.1|
cellulase M and related proteins [Pseudomonas sp. SHC52]
3.2.1.4
K01179
contig15599
2
1e-130
78%
gb|KFE35873.1|
beta-mannanase [Thioclava sp. 13D2W-2]
3.2.1.4
K01179
GH45
K01179
contig16900
8
4e-87
99
ref|WP_013834477.1|
cellulase [Novosphingobium sp. PP1Y]
3.2.1.4
K01179
contig17048
28
5e-92
63
ref|WP_033074187.1|
cellulase [Sphingopyxis sp. MWB1]
3.2.1.4
K01179
contig35
81
0.0
98
ref|WP_007809915.1|
beta-glucosidase [Rhodanobacter spathiphylli]
3.2.1.21
contig4673
11
0.0
96
ref|WP_003043551.1|
glucan 1,4-beta-glucosidase [Sphingopyxis sp. MC1]
3.2.1.21
K05350
contig6333
3
1e-09
84
gb|EXF42942.1|
beta-glucosidase/beta- galactosidase [Pseudomonas sp.
BAY1663]
3.2.1.21
K05350
contig9076
2
5e-38
74
gb|KFL30738.1|
beta-glucosidase [Devosia riboflavina]
3.2.1.21
K05350
contig17135
84
2e-58
99
ref|WP_013837096.1|
glycoside hydrolase [Novosphingobium sp. PP1Y]
3.2.1.21
K05350
GH1
K05350
contig16256
4
1e-63
93
gb|KFE35396.1|
beta-glucosidase [Thioclava sp. 13D2W-2]
3.2.1.21
K05350
contig16960
12
1e-120
69%
gb|EZP55783.1|
beta-glucosidase [Sphingomonas sp. RIT328]
3.2.1.21
K05350
contig17081
23
1e-142
73
dbj|GAL99832.1|
putative beta-glucosidase [Sphingomonas parapaucimobilis
NBRC 15100]
3.2.1.21
K01181
contig15645
1
1e-165
77
ref|WP_010130644.1|
beta-glucosidase [Microbulbifer agarilyticus]
3.2.1.21
K01181
contig16900
8
0.0
99
ref|WP_013834475.1|
1,4-beta-D-glucan glucohydrolase [Novosphingobium sp.
PP1Y]
3.2.1.21
K01181
contig17094
33
0.0
99
ref|WP_013836376.1|
beta-glucosidase [Novosphingobium sp. PP1Y]
3.2.1.21
K01188
contig17116
36
0.0
88
ref|WP_011541319.1|
beta-glucosidase [Sphingopyxis alaskensis]
3.2.1.21
K05349
contig8953
2
1e-63
82
ref|WP_029145438.1|
beta-D-glucosideglucohydrolase [Microbacterium luticocti]
3.2.1.21
K05349
contig16938
13
0.0
74
ref|WP_022678316.1|
beta-glucosidase [Novosphingobium sp. B-7]
3.2.1.21
K05349
contig15324
1
2e-88
63
ref|WP_027621620.1|
beta-glucosidase [Clostridium] clariflavum]
3.2.1.21
K05349
contig17130
70
1e-179
99
ref|WP_013836878.1|
beta-glucosidase [Novosphingobium sp. PP1Y]
3.2.1.21
contig10018
1
1e-63
79
gb|EOS54580.1|
beta-glucosidase [Paenibacillus barengoltzii G22]
3.2.1.21
GH3
K05349
K05349
contig1835
1
1e-144
100
ref|WP_010659542.1|
beta-mannosidase [Ochrobactrum anthropi]
3.2.1.25
GH77
K01192
September 2013 ⎪ Vol. 23 ⎪ No. 9