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Electron Microscope Facility
Facilities are shared with the Nicastro lab.
FEI Morgagni 286
A 100 kV scope with a tungsten filament electron
beam source and an AMT lens-coupled 1k x 1k CCD
camera (bottom mounted). This microscope is used
regularly for routine sample screening.
Philips CM12
A 120 kV scope with a LaB6 electron source and a
Gatan UltraScan 1000 2k x 2k CCD camera (bottommounted). It is cryo capable and has is used for
collecting data from negatively stained and cryo
specimens, and for collecting electron diffraction data
from 2D crystals on CCD. Because this microscope
operates at a lower voltage than the F30 (see below),
this instrument is often used for collecting cryo-EM
data from specimens for which high contrast rather
than high resolution is desired.
FEI Tecnai F20
A 200 kV scope with a field emission electron source
and a Gatan UltraScan 4000 4k x 4k CCD camera
(also bottom-mounted). The F20 is used mainly for
single particle cryo-EM data and plastic section
tomography.
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FEI Tecnai F30
A 300 kV scope with a field emission electron source,
an FEI Falcon 4k x 4k direct electron detector (bottommounted) and a Gatan Image Filter (GIF 2000) with a
2k x 2k CCD camera (also bottom-mounted). The F30
is used mainly for single particle cryo-EM data
collection. Moreover, it is used to collect electron
tomography data on the GIF.
Gatan Cp3 Cryo Plunger
A semi-automated cryo-plunger.
FEI Vitrobot Mark II
A semi-automated cryo-plunger.
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Home-Made Cryo Plunger
Compressed air driven plunger with foot switch control.
Emitech Glow-Discharge Units
Two glow-discharge units to treat carbon-coated grids.
One of the units is reserved for amylamine while the
other unit is used with all other gases.
Edwards Carbon Coater
An evaporator with an oil diffusion pump system. It is
routinely used for making high quality carbon film.
Gatan Dry Pumping Stations
Two pumping stations for Gatan cryo holders. The lab
has five Gatan cryo holders that are used with the
CM12 and F30 microscopes.
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Zeiss SCAI Flatbed Scanner
A photographic film scanner. It is used to digitize
images recorded on film plates.
Nikon Coolscan 9000ED
A photographic film scanner with a modified film holder
to accommodate photographic film plates used with
electron microscopes. This scanner is mainly used as
a backup for the Zeiss SCAI scanner.
Bench Top Diffractometer
A folded beam bench top-style diffractometer. It has a
laser beam and an LED to display a diffraction pattern
and real space image of electron micrographs at the
same time. No darkroom is needed for its operation.
Cryo Grid Storage System
A LN2 auto-filling system that is dedicated for storing
cryo grids. It has 54 high cylinders, each of which can
hold four 50ml tubes. More information about currently
stored grids can be found here.
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Linux Computer Cluster
A 372 core computer cluster for heavy-duty
computing. This cluster is dedicated to EM image
processing and only used by the Grigorieff and
Nicastro labs. 30 TB of regularly backed up redundant
disk space is available for data storage.
Source URL: http://grigoriefflab.janelia.org/facilities
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