Environ. Chem. 2016, 13, 457–463
doi:10.1071/EN15132_AC
©CSIRO 2016
Supplementary material
Toxicity of engineered copper (Cu0) nanoparticles to the green alga
Chlamydomonas reinhardtii
Emanuel Müller,A,B Renata BehraA,B and Laura SiggA,B,C
A
Eawag, Swiss Federal Institute of Aquatic Science and Technology,
CH-8600 Dübendorf, Switzerland.
B
ETH (Eidgenössische Technische Hochschule)Zürich, Swiss Federal Institute of Technology,
Institute of Biogeochemistry and Pollutant Dynamics, CH-8092 Zürich, Switzerland.
C
Corresponding author: [email protected]
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Environ. Chem. 2016
doi:10.1071/EN15132_AC
©CSIRO 2016
600
0
-5
500
400
-15
300
-20
-25
200
Zeta Potential (mV)
Z-average size (nm)
-10
65mg/L Z-ave
19.5mg/L Z-ave
5.2mg/L Z-ave
1.3mg/L Z-ave
65mg/L ZP
19.5mg/L ZP
-30
5.2mg/L ZP
100
1.3mg/L ZP
-35
0
-40
0
50
100
150
200
250
300
350
400
Exposure time (min)
Fig. S1.
Time dependence of Z-average size and zeta potential at four different concentrations of CuNPs
0.9
18
0.8
16
0.7
14
0.6
12
0.5
10
0.4
8
0.3
6
0.2
4
0.1
2
0
0
500
1000
1500
Mean number percenWDJH (%)
Number of particles (106 m/-1)
(Batch 1).
Nanosight
DLS
0
2000
Particle size (nm)
Fig. S2.
Size distribution of CuNPs (Batch 2) as number of particles and percentage of particles by DLS and
NTA.
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Environ. Chem. 2016
doi:10.1071/EN15132_AC
Fig. S3.
©CSIRO 2016
Effects of dissolved CuII (a); and of CuNPs (Batch 2) (b) on photosynthetic yield of C. reinhardtii at two
different cell densities (high cell density: 1 × 106 cells mL–1; low cell density: 0.85 × 105 cells mL–1).
Fig. S4.
Cu concentration in medium after algae exposure to dissolved Cu at high or low cell density after 1 or
2 h.
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