N- P7001
N+ P7001
N- P7001
N- P216
N+ P7001
N+ P216
Supplementary Figure S1. The phenotypes of wheat line P7001 and P216. Small yellowish spots
appear dispersedly on the leaves of P7001 under deficiency of nitrogen fertilizer (N-), while the
spots were not observed in P7001 under the sufficient nitrogen fertilizer (N+). The yellowish spots
do not appear in P216 irrespective of nitrogen dosages.
a
b
3,226
136,166
LM+
4,466
LM-
log10(FPKM of NRHRL+)
Up regulated
Down regulated
Log10 (FPKM of LM-)
Supplementary Figure S2. The expression analyses of transcripts. a) Venn
diagram of transcripts unique and common to show the number of the gene expressed
in LM+ and LM- bulks; b) Scatter plot showing the differentially expressed genes
between LM+ and LM- bulks.
Periplasm
Cytoplasm
Supplementary Figure S3. Ferredoxin-nitrite reductase (NiR) was
significantly up-regulated in the Nitrogen metabolism pathway in
LM+ bulk.