THE CRYOTIN AND PENZYME TECHNOLOGIES. USING COLD-ADAPTED MARINE ENZYMES IN
FOODS, NATURAL PRODUCTS, COSMETICS AND PHARMACEUTICALS
Jon Bragi Bjarnason
Department of Biochemistry, Science Institute, University of Iceland.
Penzyme (cod trypsin) is presently used as the active ingredient in a
patented process to produce the natural skin care formulation PENZIM
MATRIX OF PRODUCTS
CLINICAL TRIALS ARE NEEDED
• Essential for marketing purposes and for the development of pharmaceuticals
Natural products o
cosmeceuticals
COSMETICS
DRUGS
ACTIVE
INGREDIENTS
PENZYME FOR
PENZIM IN USA
PENZYME
PENZYME
BULK
PRODUCTS
PENZIM IN UK
COSMEZYME
Mask,Skin, Clean
Dr. Bragi´s Cosm.
CONSUMER
READY
PRODUCTS
PENZIM IN
KOREA & ICEL.
OCEAZYM IN Fr.
for alternative and complementary treatment of various skin
conditions, inflammation, pain and for wound healing, as well as for
• Results of clinical trials from Italy are positive for UV radiation burns, erythema (sunburn)
facial cosmetic formulations, and potentially pharmaceuticals for
• Very positive results for wound healing from the University of Malta
various indications. We refer to these processes and uses as the
• More clinical trials are planned in the near future, both in Iceland, France, UK and India
Penzyme technology (EP Patent 1 202 743 B1; US 6,846,485 B2).
WOUND HEALING STUDY AT THE UNIVERSITY OF MALTA
THE SAGA
Figure 3.35: Chart showing the preferences of the nursing staff when asked to make a
personal choice between these wound care products
More than 30 years of research on cold-adapted Cod Serine Proteases (CSPs)
from the Atlantic cod has lead to novel products & commercial applications in areas
of natural products, cosmetics and drugs.
Elastase stability in Cryotin G (26 U/ml)
120
X
X
X
100
80
60
RT
40
4°C
20
-20°C
0
0
100
200
300
400
500
Days
Kaltostat dressing
17%
PENZIM
Granuflex dressing
8%
PENZIM - a new concept in skin care
PURITY ANALYSIS USING CHROMATOGRAPHY
PENZIM -contains super - active cod protease
Penzyme III
Mini-S column
PRODUCTS
Intrasite gel
8%
Penzim gel
63%
Povidone Iodide
solution
4%
Penzyme I
Mono – Q column
PENZIM -local and topical treatment
4
6
PENZIM gel
Lower amounts of enzymes needed due to very high catalytic efficiencies
4
3
Penzim gel
0.07
100
0.06
80
150
60
100
40
50
20
0
0
0
10
20
30
cond (%)
100
80
0.05
60
0.04
0.03
40
0.02
20
0.01
0
0
0
5
10
15
20
25
30
2.5
mL
mL
2
1.5
Convetional treatment
2
1
1
Allows the use of milder conditions to destroy residual enzyme activities due to
heat and pH sensitivity
CONSUMER SURVEY ON PENZIM
Conventional treatment
3
mean length (cm)
ADVANTAGES OF CSPs USE
reduction in volume of wound (cm3)
3.5
5
120
200
Cond (%)
4.5
7
280 nm(AU)
cond%
250
Cond%
Figure 3.6: Graph showing mean length (y-axes) vs number of weeks (x-axes) for patients
who participated in the study until wound was healed or study terminated (n=33)
Figure 3.4: Graph showing reduction in volume of pressure sore (cm ) vs the number of
weeks for which PENZIM 500 gel or conventional treatment was used, for patients who
participated in the study until wound was healed or study terminated (n=33)
GUTS
mAU (280nm)
mA U (280nm)
3
AU (280nm)
RESEARCH
STABILITY OF PENZYME
Residual activity (%)
INTRODUCTION
0.5
0
0
0
0
5
10
15
20
25
5
10
15
30
20
25
30
number of weeks
number of weeks
Penetration through skin and reduction of Disease Associated Proteins (DAPs)
THERAPEUTIC POSSIBILITIES OF PENZYME FORMULATIONS
Figure 3.8: Graph showing mean width (y-axes) vs number of weeks (x-axes) for patients
who participated in the study until wound was healed or study terminated
(n = 33)
Inflammation and pain:
4
3.5
THE PENZYMES
Osteoarthritis, rheumatoid arthritis, fibromyalgia, muscle aches, trigger
Penzim gel
Conventional treatment
3
points, tendinitis, tendosynovitis, phlebitis, lymphangitis, varicose veins
and hemorroids.
mean width
2.5
Laboratory results revealed that in cell culture:
2
1.5
Cod trypsin degrades better than other proteases numerous proteins associated
with inflammation, arthritis, psoriasis, eczema, rashes etc. such as:
- TNF-alpha, IL-1 and other cytokines; MMP´s; Cell surface receptors (CD´s)
of immune system cells
- Bacterial toxins
- Fungal, bacterial and viral receptors
1
MOST RECENT PENZIM PRODUCT
0.5
Eczema and various skin indications:
Psoriasis, eczema, such as child eczema, contact dermatitis, seborrhea,
0
0
5
10
15
20
25
30
allergies to nickel, yeast, milk, chemicals, pharmaceuticals and others,
number of weeks
various skin rashes, dry, sore or chapped skin, due to diabetes, weather
or other causes, acne and boils, warts, itching and burning skin, insect
bites.
HYPOTHESIS
Wounds and injuries:
The CSPs have therapeutic activities and these therapeutic activities are due to
their ability to penetrate skin and cleave DAPs (Disease Associated Protein):
Wounds, sores from burns, cuts incisions, cold sores and shingles, sores
caused by chemical burns; swelling, muscle aches, pain caused by injury or
accident, such as sprains or broken bones, athletic injuries of various kind.
Cell surface receptors
Inflammatory mediators
Bacterial toxins
Fungal, bacterial and viral receptors
But do they penetrate and cleave DAPs in vivo?
Fungal infections:
Candida infections, athlete’s foot.
A
B
MOST RECENT COSMETIC PRODUCT
CONCLUSIONS
CLINICAL OBSERVATIONS
In this investigation we address the foundation for the pharmaceutical
Results on 250 volunteers show efficacy for:
efficacy of the marine enzyme preparation Penzyme being based on its
ability to cleave and thus reduce the concentration of pathologica
• Osteoarthritis
C
• Tendinitis
• Fibromyalgia
• Rheumatoid arthritis
A: Photograph showing a pressure sore on the dorsal aspect of the foot before
initiation of treatment with Penzim500 gel
proteins such as Interleukin 1, Tumor Necrosis Factor alpha, the cell
adhesion molecules of the immune system CD4, CD8, CD54, CD102 and
others in cell cultures.
Furthermore, we describe some results of clinical investigations
• Phlebitis
pointing to the efficacy of Penzyme formulations on osteoarthritis,
• Psoriasis
B: Photograph showing the same pressure sore on the dorsal aspect of the foot
rheumatoid arthritis, tendonitis, fibromyalgia, phlebitis, psoriasis, eczema,
• Eczema
after 15 weeks of treatment with Penzim500 gel
• Dermatitis
• Acne
C: Photograph showing the healed pressure sore after 24 weeks of treatment with
• Wound healing
Penzim500 gel
dermatitis, UV radiation burns, pressure wounds, acne and scars.
Unique and important characteristics of the Penzyme technology are
its very broad function, its marine origin (unrelated species and distant
environment) and its potential for topical and local treatment of various
indications.