Photosynthesis and Cellular Respiration Outline I. Photosynthesis A. Introduction B. Reactions II. Cellular Respiration A. Introduction B. Reactions Review: What is ATP? Review: What is ATP? Adenosine Triphospate Adenine + Ribose + 3 Phosphate groups One of the principle chemical compounds that cells use to STORE and RELEASE energy. Storing Energy ADP (Adenine diphospate) Only 2 phosphates (hence the “di”) Key to how living things STORE energy! (When energy is available, the cell stores it by adding phosphate to make ATP=stored battery ready to power cell). Releasing Energy “Shooting off” 1 phosphate ENERGY!! ATP provides energy for a variety of cellular activities! Ex: active transport across cell membranes, protein synthesis, and muscle contraction ADP ATP Cells regenerate ATP from ADP as needed by using energy in FOOD, aka … ADP ATP Cells regenerate ATP from ADP as needed by using energy in FOOD, aka … GLUCOSE! Photosynthesis Photo = light Synthesis = putting together Photosynthesis Converting sun energy into chemical energy usable by cells (glucose) Autotrophs: self feeders, organisms capable of making their own food – – Photoautotrophs: use sun energy e.g. plants photosynthesis-makes glucose from light Chemoautotrophs: use chemical energy, NOT sunlight e.g. bacteria that use sulfide or methane Photosynthesis Photosynthesis takes place in specialized structures inside plant cells called chloroplasts – Light absorbing pigment molecules e.g. chlorophyll Why are leaves green? Chlorophyll absorbs all of the light waves except green. Because the green is not absorbed, it is reflected, so that is the only color wavelength our eye can pick up. Accessory pigments – help to fill in this gap – – Carotenoids – orange (like carrots) Xanothphyl – yellow Inside the chloroplast Thylakoids: Sac-like photosynthetic membranes arranged in stacks. – Organize chlorophyll/other pigments into photosystems (clusters that collect light). What does a plant need to survive? Overall Reaction 6CO2 + 6H2O + light energy → C6H12O6 + 6O2 Carbon dioxide + water + light glucose + oxygen KNOW BOTH OF THESE EQUATIONS. YOU WILL SEE THEM AGAIN! 2 types of reactions in photosynthesis Light dependent Light independent (CALVIN CYCLE) Electron Carriers Overview: – – – – – Sun excites electrons = gain energy Need special carrier! (Think red-hot coal!) Electron acceptor molecules (ex: NADP+) NADP+ uses hydrogen ions (H+) to trap some of the sunlight in chemical form = converts to NADPH. Carries e- to other chemical reactions in the cell Light Dependent Reactions Occur in the thylakoid! Produce oxygen gas Converts ADP and NADP+ into energy carriers ATP + NADPH Light Dependent Reactions Overview: Pigments in Photosystems absorb light = electrons gain energy Electrons are passed on to the Electron Transport Chain Does the chorophyll run out of electrons? Light Dependent Reactions In Thylakoid: – – – System that provides new e- to chlorophyll to replace ones lost Electrons come from water! H2O Enzymes on inner surface membrane of thylakoid break up each H2O into: 2 e- (replace e- lost to the ETC) 2H+ions (released inside thylakoid membrane) 1 oxygen atom (released into the air!) Light-dependent Reactions 5. H+ ions cant cross membrane directly – ATP synthase! Allows H+ ions to pass through it! As it turns, binds ADP and phosphate group …. Producing ATP! Energy Shuttling Recall ATP: cellular energy-nucleotide based molecule with 3 phosphate groups bonded to it, when removing the third phosphate group, lots of energy liberated= superb molecule for shuttling energy around within cells. Other energy shuttles-coenzymes (nucleotide based molecules): move electrons and protons around within the cell NADP+, NADPH NAD+, NADP FAD, FADH2 This light dependent reaction is the source of nearly ALL of the oxygen in Earth’s atmosphere! Thanks photosynthesis! Light-dependent Reactions Photosystem: light capturing unit, contains chlorophyll, the light capturing pigment Electron transport system: sequence of electron carrier molecules that shuttle electrons, energy released to make ATP Electrons in chlorophyll must be replaced so that cycle may continue-these electrons come from water molecules, Oxygen is liberated from the light reactions Light reactions yield ATP and NADPH used to fuel the reactions of the Calvin cycle (light independent or dark reactions) Uses: H2O, ADP, and NADP+ Makes: Oxygen, ATP, and NADPH Calvin Cycle (light independent or “dark” reactions) ATP and NADPH generated in light reactions used to fuel the reactions which take CO2 and H+ to make glucose. Simplified version of how carbon and energy enter the food chain Harvesting Chemical Energy Plants and animals both use products of photosynthesis (glucose) for metabolic fuel Heterotrophs: must take in energy from outside sources, cannot make their own e.g. animals When we take in glucose (or other carbs), proteins, and fats-these foods don’t come to us the way our cells can use them ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration CO2 and O2 Water Glucose Location: Where does it take place? Light ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored CO2 and O2 Water Glucose Location: Where does it take place? Light ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored CO2 and O2 Water Glucose Location: Where does it take place? Light Uses CO2; Produces O2 ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored CO2 and O2 Water Glucose Location: Where does it take place? Light Uses CO2; Produces O2 Uses water ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored CO2 and O2 Water Glucose Location: Where does it take place? Light Uses CO2; Produces O2 Uses water Produces glucose from energy ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored CO2 and O2 Water Uses CO2; Produces O2 Uses water Glucose Produces glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Light ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored CO2 and O2 Water Uses CO2; Produces O2 Uses water Glucose Produces glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) Cellular Respiration Overview Transformation of chemical energy in food into chemical energy cells can use: ATP These reactions proceed the same way in plants and animals. Process is called cellular respiration Overall Reaction: – C6H12O6 + 6O2 → 6CO2 + 6H2O Cellular Respiration Overview Breakdown of glucose begins in the cytoplasm: the liquid matrix inside the cell At this point life diverges into two forms and two pathways – – Anaerobic cellular respiration (aka fermentation) Aerobic cellular respiration C.R. Reactions Glycolysis – – – – Series of reactions which break the 6-carbon glucose molecule down into two 3-carbon molecules called pyruvate Process is an ancient one-all organisms from simple bacteria to humans perform it the same way Yields 2 ATP molecules for every one glucose molecule broken down Yields 2 NADH per glucose molecule Anaerobic Cellular Respiration Some organisms thrive in environments with little or no oxygen – Marshes, bogs, gut of animals, sewage treatment ponds No oxygen used= ‘an’aerobic Results in no more ATP, final steps in these pathways serve ONLY to regenerate NAD+ so it can return to pick up more electrons and hydrogens in glycolysis. End products such as ethanol and CO2 (single cell fungi (yeast) in beer/bread) or lactic acid (muscle cells) Aerobic Cellular Respiration Oxygen required=aerobic 2 more sets of reactions which occur in a specialized structure within the cell called the mitochondria – – 1. Kreb’s Cycle 2. Electron Transport Chain Kreb’s Cycle Completes the breakdown of glucose – – Takes the pyruvate (3-carbons) and breaks it down, the carbon and oxygen atoms end up in CO2 and H2O Hydrogens and electrons are stripped and loaded onto NAD+ and FAD to produce NADH and FADH2 Production of only 2 more ATP but loads up the coenzymes with H+ and electrons which move to the 3rd stage Electron Transport Chain Electron carriers loaded with electrons and protons from the Kreb’s cycle move to this chainlike a series of steps (staircase). As electrons drop down stairs, energy released to form a total of 32-34 ATP Oxygen waits at bottom of staircase, picks up electrons and protons and in doing so becomes water Energy Tally 34-36 ATP for aerobic vs. 2 ATP for anaerobic – Glycolysis 2 ATP – Kreb’s 2 ATP – Electron Transport 32-34 ATP 36-38 ATP Anaerobic organisms can’t be too energetic but are important for global recycling of carbon Photosynthesis vs. Cellular Respiration Comparing/Contrasting worksheet! ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored Energy is released CO2 & O2 Water Uses CO2; Produces O2 Uses water Glucose Produces glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored Energy is released CO2 & O2 Water Uses CO2; Produces O2 Uses water Glucose Produces glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) Uses O2; Produces CO2 ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored Energy is released CO2 & O2 Water Uses CO2; Produces O2 Uses O2; Produces CO2 Uses water Produces Water Glucose Produces glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored Energy is released Uses CO2; Produces O2 Uses O2; Produces CO2 Uses water Produces Water Glucose Produces glucose from energy Burns glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms CO2 & O2 Water Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored Energy is released Uses CO2; Produces O2 Uses O2; Produces CO2 Uses water Produces Water Glucose Produces glucose from energy Burns glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Glycolysis: Cytoplasm of eukaryotic, prokaryotic cells CO2 & O2 Water Aerobic respiration: mitochondria of ALL eukaryotes Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) ENERGY Photosynthesis Cellular Respiration Photosynthesis vs. Cellular Respiration Energy is stored Energy is released Uses CO2; Produces O2 Uses O2; Produces CO2 Uses water Produces Water Glucose Produces glucose from energy Burns glucose from energy Location: Where does it take place? Chloroplasts of green plants, algae, and some microorganisms Glycolysis: Cytoplasm of eukaryotic, prokaryotic cells CO2 & O2 Water Aerobic respiration: mitochondria of ALL eukaryotes Light Requires light (light-dependent reaction; Calvin Cycle can occur in light or dark.) Occurs in dark and light
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