Curriculum Vitae
PERSONAL INFORMATION
Andrea Nesler
Andrea Nesler
12, loc. Visintainer, Pergine Valsugana, 38057 (Italy)
[email protected]
[email protected]
Sex Male | Date of birth 04/01/1985 | Nationality Italian
POSITION
Post-Doc
WORK EXPERIENCE
From 28/02/2015 to 31/08/2016
Development Assistant
Bi-PA (Biological Products for Agriculture) Technologielaan 7, B-1840 Londerzeel (Belgium)
Secondment in Bi-PA (through the European project Innova) focused on the development of
innovative bio-based pesticides to minimize chemical residue risk on food.
Sector Microbiology, Plant protection, Biocontrol
From 01/02/2014 to 28/02/2015
Postdoctoral position
Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach, 1 38010 S. Michele
all'Adige (TN) - ITALY
Postdoctoral position on the European project entitled “Pesticide Use-and-risk Reduction in European
farming systems with Integrated Pest Management”
Sector Molecular biology, Microbiology, Plant protection
EDUCATION AND TRAINING
From 01/01/2010 to 18/04/2013
Ph.D. in molecular, industrial and environmental biotechnologies
University of Verona , via dell'Artigliere 8 37129 Verona (Italy)
Genetics, molecular biology, phytoremediation
Doctoral thesis: Modulating heavy metal accumulation in plants: overexpression of
the Pseudomonas putida efflux complex CzcCBA
© European Union, 2002-2013 | http://europass.cedefop.europa.eu
Page 1 / 6
Curriculum Vitae
From 01/10/2007 to 14/07/2009
Andrea Nesler
Master’s degree in Food Biotechnology (110/110 cum laude)
University of Padova, via 8 Febbraio 35122 Padova (Italy)
Plant biology, molecular biology, biochemistry and agronomy
Master’s thesis: Effects of grapevine polyphenols and PR proteins on the growth and laccase activity
of Botrytis cinerea
From01/09/2004 to 12/10/2007
Bachelor’s degree in Agricultural Biotechnology (105/110 )
University of Padova, via 8 Febbraio 35122 Padova (Italy)
Plant biology, physiology, biochemistry and agronomy
Bechelor’s thesis: Expression of defence genes in grapevine plants inoculated with Armillaria mellea
From 01/09/1999 to 06/07/2004
High school leaving qualification (86/100)
Liceo Scientifico "Marie Curie" (state scientific high school) 4, Via San Pietro 38057 Pergine
Valsugana (Italy)
PERSONAL SKILLS
Mother tongue(s)
Italian
Other language(s)
English
UNDERSTANDING
SPEAKING
WRITING
Listening
Reading
Spoken interaction
Spoken production
B2
B2
B2
B1
B1
Levels: A1/2: Basic user - B1/2: Independent user - C1/2 Proficient user
Common European Framework of Reference for Languages
Communication skills
Good ability to comunicate and work with others in multicutural and multidisciplinary teams.
▪ Comunication skills gained during the research experiences (Master’s thesis and Ph.D.)
© European Union, 2002-2013 | http://europass.cedefop.europa.eu
Page 2 / 6
Curriculum Vitae
Job-related skills
Andrea Nesler
Excellent knowledge of the most common tools used in a biology/biochemistry laboratory and
techniques of molecular/cellular biology and microbiology. Experience in the development of biocontrol
tools in agriculture. Management of trials in laboratory, greenhouse and field. Protocol preparation,
trials execution and results elaboration. Management of GEP trials performed by contractors.
Molecular biology:nucleic acids extraction, gel electrophoresis and quantification, Southern and
Northern blot, PCR reaction, cloning, preparation of competent cells and transformation of E. coli and
A. tumefaciens, RNA reverse-transcription, non quantitative RT-PCR and Real Time RT-PCR, RACEPCR. Samples preparation for 454 (Roche) sequencing of microbial communities.
Microbiology: good command of microbiology general techniques and maintenance of cell cultures.
Preparation of microbiological media, sterilisation, aseptic techniques, inoculation, incubation,
streaking, dilution plating, pure culture technique. Functional characterization of bacteria based on
proteolytic activity, siderophores production. Assessment of the efficacy of biological control agents to
control plant pathogens in vitro and in vivo.
Bioinformatics: Sequence alignment and homology study, database search.
Knowledge on the most common web-tools used in Bioinformatics like: BLAST,
FASTA and some protein databanks.
Plant cell cultures: A. tumefaciens-mediated stable and transient transformation of plant tissues
(transformation of Nicotiana tabacum, Arabidopsis thaliana, Lycopersicon esculentum), in vitro
propagation and culture. Preparation of protoplasts (Nicotiana benthamiana and Arabidopsis thaliana)
and transient transformation with fusion constructs.
Plant pathology: infections with several plant pathogens (in vitro and in vivo experiments), diseases
assessment. Execution of trials in open field conditions. Compatibility test between plant protection
products.
Agronomy: basic knowledge about the common agricultural practices of the main crops in Europe
especially for viticulture.
Biochemistry: protein extraction and quantification, SDS-PAGE and 2D-PAGE, western blot.
Microscopy: good experience in the use of optical microscopes. Short research experience in
fluorescence microscopy.
Computer skills
Driving licence
Excellent knowledge of the OFFICE pack (Word, Excel, Power Point, Access) and of the common
web tools of the bioinformatics disciplines like BLAST, FASTA and pdbviewer. ECDL Core
I worked with the program ARM (Agricultural Research Manager).
▪B
© European Union, 2002-2013 | http://europass.cedefop.europa.eu
Page 3 / 6
Curriculum Vitae
Andrea Nesler
ADDITIONAL INFORMATION
Education activities
Tutoring in laboratory class of Genetic methodologies in the Biotechnology bachelor’s degree,
University of Verona (March-June 2011, 2012, 2013).
Research Experience
Doctoral thesis: Modulating heavy metal accumulation in plants: overexpression of the
Pseudomonas putida efflux complex CzcCBA
Supervisor: Prof. Antonella Furini
Main reasearch activities: generationof transgenic plants (Arabidopsis thaliana, Nicotiana tabacum,
Lycopersicon eslulentum) able to modify the heavy metal accumulation in their tissues, by
overexpression of the bacterial efflux complex czcCBA. The goal of this project was to transfer a
bacterial transport system into plant in order to obtain transformants with a modified heavy metal
accumulation. Overexpressing the whole czcCBA complex was obtained in tobacco and a strong
reduction of Cd content was measured in shoots in comparison to wild type plants.
Master’s thesis: Effects of grapevine polyphenols and PR proteins on the growth and laccase activity
of Botrytis cinerea
Supervisor: Prof. Francesco Favaron
Co-supervisors: Dr. Luca Sella
Main reasearch activities: Study of the interaction between the pathogen B. cinerea and the defensive
compounds of grape berry. By in vitro interaction experiments we analyzed the mechanisms of
pathogen evasion of plant defenses. We hypothesized that pathogen laccase (oxidase enzyme)
catalyzes a series of coupled reactions of berry phenols causing the formation of complex between
resveratrol and other phenols avoiding in this way the development of trans-viniferin the toxic dimer of
resveratrol.
Bechelor’s thesis: Expression of defence genes in grapevine plants inoculated with Armillaria mellea
Supervisor: Prof. Francesco Favaron
Co-supervisors: Dr. Claudio Moser and Dr. Michele Perazzolli
Field of research: Study of defense genes upregulated in response to Armillaria mellea infection.
Specific defence genes such as protease inhibitors, a thaumatin and a tumour related protein resulted
strongly induced in the rootstock Kober 5BB, 24 h after pathogen inoculation. The upregulation was
validated in repeated experiments in vitro and under greenhouse controlled conditions.
© European Union, 2002-2013 | http://europass.cedefop.europa.eu
Page 4 / 6
Curriculum Vitae
Andrea Nesler
PUBLICATIONS
Peer-reviewed ISI-WOK publications
Nesler A., Perazzolli M., Puopolo G., Giovannini O., Elad Y. and Pertot I. (2015) A complex protein
derivative acts as biogenic elicitor of grapevine resistance against powdery mildew under field
conditions. Front Plant Sci. 2015; 6: 715.
Published online 2015 Sep 18. doi: 10.3389/fpls.2015.00715
ISBN/ISSN publications
Raiola A., Castiglioni C., Nesler A., Elmaghraby I. and Favaron F. (2009). Identification of amino acid
residues of Fusarium verticillioides endo-polygalacturonase required to escape the inhibition by host
plant PGIP. In: Journal of Plant Pathology (4, Supplement) Locorotondo (Bari), 28 settembre - 1
ottobre 2009 Vol. 91, p. S4.83, (ISBN/ISSN: 1125-4653)
Perazzolli M., Bampi F., Nesler A., Faccin S., Ciccotti A.M., Gessler C., Pertot I., and Moser C. (2009).
Functional characterization of grapevine defence genes induced during Armillaria mellea root infection
identified a specific antifungal protein. IOBC-WPRS Bullettin 43: 125-128.
Book chapters:
Nesler A. and Antonella Furini (2012). “Phytoremediation: The Utilization of Plants to Reclaim Polluted
Sites” In Plants and Heavy Metals. Antonella Furini (ed.) First Edition. Springer Netherlands, 2012
Congress participations:
X International Symposium on Grapevine Physiology and Biotechnology
Verona, June 13 – 18 2016
Giornate Fitopatologiche
Chianciano Terme, 8 -11 March 2016
The 67th International Symposium on Crop Protection
Ghent, May 19th, 2015.
10th International PGPR Workshop
Liege, Belgium 16-19 June 2015
List of Abstracts and Oral Communication (The speaker is underlined)
Nesler A., Perazzolli M., Antonielli L., Cappelletti M., Giovannini O., Pindo M., Puopolo G. And Pertot I.
A complex protein derivative acts as biogenic elicitor of grapevine resistance against powdery and
downy mildews. Oral communication and Abstract, 68th International Symposium on Crop Protection
Ghent, May 17th, 2016.
Perazzolli M., Nesler A., Antonielli L., Cappelletti M., Torboli E., Pindo M., Puopolo G. and Pertot I.
(2015). Improvement of the natural phyllosphere microbiota of the grapevine to increase resistance
against downy mildew. Oral communication and Abstract, 10th International Symposium on
Phyllosphere Microbiology 19-29 July, Ascona, Switzerland.
List of Abstracts and Poster presentations
Perazzolli M., Nesler A., Torboli E., Giovannini O., Puopolo G. and Pertot I. (2015). Grapevine
resistance induced by protein hydrolysates against mildews. Poster communication, Joint International
Workshop on: PR-Proteins and Induced Resistance Against Pathogens and Insects. 6-10 September,
Aachen, Germany.
Nesler A., Dal Corso G., and Furini A. (2011) Controlling cadmium accumulation in plants. What's for
lunch? Nutrients and minerals in every day food. How the knowledge on mineral nutrition of plants can
improve human nutrition. Poster communication, Second Annual Conference and MC Meeting Cost
Action FA 0905
Venezia, Palazzo Cavalli-Franchetti, 23-26 November 2011
© European Union, 2002-2013 | http://europass.cedefop.europa.eu
Page 5 / 6
Curriculum Vitae
Andrea Nesler
Nesler A., Dal Corso G., and Furini A. (2011). Controlling heavy metal accumulation in plants.
Poster communication, Proceedings of the Joint Meeting AGI-SIBV-SIGA
Assisi, Italy – 19th-22th September 2011 (ISBN 978-88-904570-2-9)
I consent to the use of my personal data in accordance with the provisions of decree 196/2003.
© European Union, 2002-2013 | http://europass.cedefop.europa.eu
Page 6 / 6