PROPOSAL TO CREATE A CENTER FOR MOLECULAR PLANT SCIENCES
I. Long-term Objective. The success of the interdisciplinary Plant Molecular
Biology/Biotechnology (PMBB) Program has evolved to the point that a College-wide
(CBMPS and CFAES) Center for Molecular Plant Sciences is needed to better focus
interdisciplinary efforts in the molecular plant sciences at OSU. A formalized Center
would be a natural and desired progression from the successful interdisciplinary dualcollege PMBB Program effort initiated nearly 15 years ago.
II. Introduction. Basic plant sciences at The Ohio State University, particularly
molecular-based studies, are very strong and are highly ranked amongst land-grant
universities. The majority of faculty conducting plant-related research reside in various
departments of the new College of Biological, Mathematical and Physical Sciences
(CBMPS) and College of Food, Agricultural, and Environmental Sciences (CFAES).
Other colleges, i.e., Pharmacy, Medicine, and Engineering, also contribute to the overall
excellence in plant-related research on campus. Several years ago (mid 1990’s), the
interdisciplinary Plant Molecular Biology/Biotechnology (PMBB) Program was created to
provide leadership in accelerating the trend towards interdisciplinary plant molecular
biology research. To date, PMBB is a voluntary association created by a grass-roots
effort of faculty from both Colleges. The Deans and Central Administration provided
strategic support for the PMBB Program at its inception. PMBB, along with the Plant
Biotechnology Center, and the Plant-Microbe Genomics Facility, now consists of a
vibrant, productive, and collegial community of 29 plant-related researchers. Early on,
PMBB successfully competed for positions supported by the Molecular Life Sciences
initiative. The resulting 6 strong hires in turn stimulated the participating departments to
contribute additional positions. This resulted in the two Colleges adding several
additional stellar scientists in the field of molecular plant science. More recently, PMBB
was awarded a Targeted Initiative in Excellence in Translational Plant Sciences (TIETPS) by OSU. This ongoing initiative has already led to the hiring of two new faculty,
with three additional faculty searches that are currently underway. Moreover, PMBB
members have garnered extremely competitive high visibility grants (from the Gates
Foundation, NSF’s 2010 Project and Plant Genome Program, USDA-NRI, DOE
Genomes to Life, and Ohio’s Third Frontier Program). The total expenditures of PMBB
member grants exceeded $9 million in FY07. Clearly, PMBB faculty members lead
active and productive research programs, also exemplified by published work in
prestigious general journals such as Cell, Nature, Science, Proceedings of the National
Academy of Sciences, Current Biology and EMBO Journal as well as top journals in their
respective fields including Plant Cell, Plant Physiology, Applied and Environmental
Microbiology, and the Journal of Biological Chemistry. PMBB members train large
numbers of graduate students and many of these individuals have played significant
roles in contributing to the overall productivity of PMBB member laboratories.
The PMBB community has also been instrumental in driving the formation of
several interdepartmental and inter-campus instrumentation centers to optimize the cost
effective productivity of the plant science community. These include the Molecular and
Cellular Imaging Center (MCIC) at the Wooster Campus, the Plant Microbe Genomics
Facility (PMGF) at the Columbus Campus, and the beginning of a Metabolomics
Laboratory branch of PMGF in Rightmire Hall. Recently another resource for shared
instrumentation was developed in Kottman Hall on Columbus Campus that includes an
additional node for genomics and metabolomics work.
Likewise, PMBB has endorsed and led several efforts over the past few years to
increase the video-linking capacity of member departments and the linking of seminars
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and teaching of classes between Wooster and Columbus campuses. This latter effort
has greatly enhanced enrollment in our classes and the productivity of our graduate
students in that they no longer are burdened by extensive travel which is disruptive to
their thesis research. Video-linking courses taught by PMBB faulty has also
tremendously enhanced the educational breath of the Wooster students, helping insure
their competitiveness in future job prospects. This video-linking capability will also play
an important role in the development of our novel international tripartite graduate
program with the University of Sao Paulo and Rutgers University (fully described in TIErelated documents).
III. Rationale. PMBB does not receive an annual budget and the one-time funding
associated with the establishment of PMBB will soon run out. As noted above, PMBB
has been a very productive venture, bringing in many new top-notch faculty with high
profile research programs and extramural funding. In addition, PMBB has served as a
focal point for the development of several interdisciplinary and cooperative research and
graduate training efforts. In light of the current university-wide strategic planning process
and the planned merger of the departments of Plant Cellular and Molecular Biology and
Molecular Genetics, we thus feel it is an appropriate time to reaffirm college
commitments to PMBB through the establishment of a more formal college-wide Center
for Molecular Plant Sciences (CMPS). The Center will maintain and strengthen the
momentum achieved by the highly productive PMBB Program by providing a
permanently funded and stable umbrella organization. The organizational structure of a
Center is critical in maintaining a strong plant molecular biology community focused on
cutting-edge basic and applied plant research and the training of graduate students. The
Center will also be an important recruitment tool when hiring future faculty and also play
an important role in retaining our current faculty. In addition, a formalized Center will be
a much more effective vehicle to mobilize other researches on campus that work with
plant systems, and whose research capabilities might be effectively merged with the
efforts of current PMBB members. For example, there is a strong natural products group
in the College of Pharmacy that works closely with current PMBB members and several
members of the PMBB group have initiated joint efforts with colleagues in Engineering.
Certainly the new divisional structure in CBMPS has already facilitated closer
collaborations between the Department of Chemistry and the current Translational Plant
Sciences TIE. This collaboration has already paid dividends in that CBMPS, CFAES and
Engineering successfully collaborated on an Ohio Research Scholars Proposal. The
ORSP submission proposed hiring a new faculty member in FAES to engineer new
plants which are rich in organic chemicals, a faculty member in synthetic Organic
Chemistry who will transform plant-derived chemicals into versatile new building blocks
and a faculty member in Engineering who can develop new methods of polymerization of
the renewable and synthetically transformed feedstock chemicals. ORSP funded two
endowed faculty positions and the three colleges will collaborate on self-funding the
third. These types of collaborations will be much more prevalent and maximized with the
establishment of a formalized Center that could focus on such joint efforts. Finally, we
also envision that a strong molecular plant science Center at OSU will provide the
needed leadership in state-wide efforts in emerging fields such as plant biotechnology,
phytopharmaceuticals, biofuels, and alternative energy-related projects.
IV. Administration of the Center/Governance and Oversight Structure. Currently,
the PMBB program is headed by a Director, who serves for a three year term,
responsible to the CBMPS and CFAES Deans. Dean Matt Platz (CBS) is Lead Dean for
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the current Translational Plant Sciences TIE, and thus is ultimately responsible for fiscal
oversight; we envision that some form of similar agreement may be established with the
two Colleges who would administer the proposed new Center. In addition, in the current
PMBB Program, a PMBB Council consisting of four PMBB faculty is elected by the
PMBB Program members. Each member of the Council serves two years before
rotating off. Membership is staggered so that two new Council members are elected to
replace two out-going members each year. This assures continuity of leadership on
Council, with the Director as an ex-officio member. The Council was established to
assist and form a communication bridge between the Director and the PMBB members.
In particular, the Council helps to organize and monitor the activities of the various
committees that are responsible for PMBB-related functions. We propose that a similar
arrangement be established to govern the new Molecular Plant Sciences Center. All
proposed actions that are to be made relative to the Center will first be vetted by the
Director and Council and then brought to the whole Center membership for discussion
and ratification. Good examples that well illustrate this approach are the new Graduate
Fellowship and SURE undergraduate research programs established by PMBB, along
with recent and current planning for the upcoming technical personnel hires and
equipment purchases through the TIE. As in PMBB, all faculty hires supported by the
Center will be accomplished using Search Committees comprised of Center members
along with other individuals from the different TIU departments. The entire Center
membership will then vote on prospective candidates. This modus operandi, in addition
to college specific policies on faculty hiring, has worked well over the years for all PMBB
activities and hires and will be continued in the future upon the establishment of the
Center. Our governance procedures are also flexible enough to easily incorporate any
changes as needed.
V. Strategic Plan and Budgetary Considerations. To implement the concept of a
Center for Molecular Plant Sciences, it will be necessary to attract a Director and obtain
stable funding for staff positions and operating expenses.
A. CMPS Director. We request funds to recruit an outside Director for the
proposed new Center (CMPS). This recruitment should be initiated as soon as possible.
In addition, the hiring of such an individual would also help to assuage the loss of other
recent senior molecular plant scientists. Indeed, the former PCMB Department recently
attempted to hire a senior-level molecular plant scientist in 2006-2007; thus the
recruitment of the Director could be looked upon as an extension of this past recruitment
effort by the College. The term of current PMBB Director Robert Tabita expires in
September of 2010. Thus, this is an excellent time to initiate the recruitment of a new
Director. We will look for an outstanding scientist with an active research program,
world-class reputation, preferably someone with administrative experience, and a person
with genuine passion to lead and help build the highest caliber interdisciplinary plant
science community. It would be desirable that this person’s research interests are in a
field related to the recent TIE. The Director will effectively develop and implement
activities to enhance interactions among the Center labs and provide visional leadership
to recruit faculty and compete for large-scale funding that will place OSU at the leading
edge of plant research. The Director will also lead collaborative efforts among the plant
community and other scientific communities on campus, such as pharmacy, chemistry,
food science, engineering, etc, to develop innovative programs addressing fundamental
medical, nutrition, and energy issues. The Director will also assist and enhance on-going
innovative programs and international collaborations between OSU and other institutions
in plant research. These collaborations are a major part of the Translational Plant
Sciences TIE and current Regents Innovation funds. it is expected that the new Director
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will take the lead in enhancing these programs and securing renewal funding when the
current TIE expires.
To attract such a person, a major investment in start-up funding, research space,
and other incentives will be required. While expensive, this new Director hire could be
viewed as offsetting the recent loss of senior molecular plant scientists. In addition,
other inducements should be considered to attract such an individual. Certainly, realized
and contemplated junior hires initiated with the current TIE should be attractive for a new
Director. Moreover, the establishment of this Center should stimulate agreements for
other future joint faculty positions that might be considered in conjunction with other
departments such as Chemistry, Engineering, Pharmacy, Nutrition, and Medicine. The
advent of such innovative position hires would serve as a strong inducement to attract
an outside Director and could be established with current resources for the hiring of
future new faculty. To provide the Director with adequate support for his/her research
program while the Director focuses on Center activities, it is recommended that the
equivalent of a senior postdoctoral research scientist position be provided to help run the
Director’s research program. In addition, it is recommended that 2 months summer
salary be provided to the Director.
B. Administrative Staff ($125,000 salary plus benefits/year).
1. Administrative Associate. We request continuing support for a full-time
Administrative Associate staff member ($85,000 salary plus benefits per year) who will
have wide ranging responsibilities including assisting the Director, serving as a grants
coordinator for Center faculty, and acting as a liaison between Center faculty and the
Office of Technology, Licensing and Contracts. Up to this point, PMBB has been
operating exclusively through the associated faculty, who have taken management
responsibilities for the program and a part-time Associate who has HR responsibilities
for other units such as PMGF and Plant Biotechnology. With the large number of faculty
associated with an expanded Center for Molecular Plant Sciences, a full-time
Administrative Associate is critical for maintaining and enhancing the productivity of the
faculty. The main responsibility for this staff member will be supporting the efforts of the
Director to coordinate Center faculty efforts and other housekeeping tasks of the Center,
such as coordinating the annual Symposium, Distinguished Seminar Program, retreat,
etc. The Administrative Associate will also serve to assist the Director and Center faculty
in assembling grant proposals which are submitted through Center team efforts. Finally,
the Administrative Associate will serve as a liaison with the Office of Technology,
Licensing and Contracts to assist Center faculty with contracts, patent applications and
licensing of IP. The Molecular Plant Sciences Center is currently poised to impact both
basic and applied plant sciences. Basic plant science is enhanced through basic
molecular plant science research, followed by information exchange within the scientific
community. Applied plant science continues to shift from the University to industry, which
has greater resources and the infrastructure to commercialize products. The role of the
university in the molecular plant sciences has changed but the creative science that
exists at the core of the Center has tremendous potential for commercialization, once IP
is secured. Center faculty have not only generated high impact publications but also hold
numerous patents, many of which have been licensed for eventual commercialization of
an industrial product. Unfortunately, some opportunities for IP have not been realized.
The faculty and the University would benefit from additional assistance in this area,
through assignment of support staff. The Administrative Associate would not be an
inventor, but would facilitate assembly of patent disclosures, assist in the submission of
data/documentation to the office of TLC, and help to identify or contact potential
licensees.
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2. Outreach and engagement specialist/graduate and undergraduate coordinator
(half-time position at $40,000 salary plus benefits/year). In a study performed by NASA
in 2000, it was shown that most children lose interest in science and math by the third
grade. The effect of this is currently being seen as the level of scientific literacy in the
general population has eroded and the number of college students entering the STEM
disciplines has declined. To address this problem, in addition to providing leadership to
Ohio’s plant molecular biology research community, the CMPS Center will have as one
of its core missions to engage K-12 students and teachers and institutions serving
primarily minority students, with the goals increasing the overall scientific literacy in the
state of Ohio, increasing the number of students at all levels entering college in a STEM
discipline, and driving up the number of women and minorities going into STEM majors
at the undergraduate and graduate level. To facilitate continual and meaningful
interactions with these students, we request continuing support for a half-time staff
position ($40,000 salary/year plus benefits) for an Outreach and Engagement
Coordinator. Duties for this position will be the following:
1. Identify opportunities within OSU and throughout the state for center faculty to
interact with K-12 students and institutions serving primarily minority or tribal
communities.
2. Work with faculty as they develop research grants to incorporate appropriate
outreach and engagement objectives that will enhance those of the center.
3. Identify key areas where center faculty can have an impacting effect on the level
of science literacy and interest in the STEM disciplines.
4. Connect Center faculty with educators throughout Ohio to foster partnerships in
STEM education in the state.
Initiatives such as this to promote STEM education are not new. The reason that
most of these initiatives have short-lived success is that they are all too often driven by
research faculty that have a limited understanding of what educators need and what
students are attracted to. Many of these types of programs often run for a few short
years where the continual interactions that are necessary to impart lasting change are
not maintained. More times than not, faculty interested in these activities spend
enormous amounts of time re-inventing the wheel and trying to identify appropriate
venues to interact. The position proposed here will be an expert in the needs of Ohio’s
science curriculum, they will be able to deploy center resources and faculty where they
can be most effective, and they will interact with and be part of the overall efforts OSU
puts towards elevating science and math in the state of Ohio. In collaboration with the
Center faculty, this position can have a lasting influence on the state of science
understanding and education in this state.
In addition to outreach and engagement, this person will also help faculty
coordinate TIE efforts in the new Translational Plant Sciences Graduate Program as well
as the Summer Undergraduate Research Experience (SURE) Program described with
our TIE documents. The outreach and extension specialist will also be valuable asset in
providing the outreach component which is required for many of the federally-funded
center grants.
C. Translational Plant Science Symposium ($50,000 per year). PMBB has
been holding an annual symposium for the past 10 years. The symposia were aimed at
facilitating interactions among the labs in PMBB, especially among graduate students
and postdoctoral fellows. The symposia have attracted other researchers from around
the state of Ohio, and we routinely have used this opportunity to invite an eminent
researcher as a plenary lecturer to kick off this Symposium. Because of limited funding,
these activities are narrow in scope and no longer meet the exciting dynamics and
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challenges of the molecular plant science community. Therefore, we propose a new TPS
Symposium series that will combine the current PMBB symposia and further expand
from a regional to international level by including participation of researchers across the
country and from other parts of the world. It will feature at least 10 invited speakers of
international caliber. Clearly, scientific meetings are essential for the advancement of
most, if not all, research fields, in terms of sharing the latest research results,
establishing collaborations, and building mutual support of each other’s work. Host
institutions for meetings can gain enhanced reputation among colleagues,
competitiveness in grant funding (especially large group grants), and exposure of their
students/postdocs to other researchers. As an important means to boost the world
prominence of OSU plant science, we request $50,000 per year as matching funds to
host an international symposium on Translational Plant Science (TPS). The matching
funds will help us seek additional funding from federal agencies such as NSF, USDA
and DOE. The scientific theme for each year will be developed based on the recent
developments in science. Examples include biofuels, biomass, disease resistance,
genomics, developmental mechanisms, plant products in medicine, human nutrition, etc.
Every symposium will strive to integrate basic and applied research, model and nonmodel plant and crop species, and invited presentations by established researchers and
rising stars. Researchers from OSU and other Ohio institutions will also be invited to
showcase research in the state. Such a symposium will also provide an opportunity to
invite and interact with our colleagues and students associated with the tripartite
graduate program with the University of Sao Paulo and Rutgers University and part of
the requested budget will be used for this purpose.
The CMPS will inaugurate the TPS series by sponsoring the Second
International Conference on Plant Vascular Biology (PVB), which will be organized and
chaired by PCMB faculty Biao Ding in 2010 on the OSU campus. At the end of the First
International PVB Conference held in May 2007 in Taipei, Taiwan, OSU was selected
among several competing proposals by the conference international committee to host
the second conference in recognition of the international leadership and the excellence
of OSU’s plant science research. Expected to be attended by approximately 300
researchers and featuring nearly 20 invited speakers from around the world, this
conference will provide an exceptional opportunity to showcase plant research as OSU,
elevating PMBB (or MPSC) visibility and leadership in plant research. The Samuel
Roberts Noble Foundation in Oklahoma has administratively promised $10,000 to help
support this conference. The Noble Foundation support and the requested OSU support
will be used as critical matching funds to seek other support from federal agencies
including USDA, NSF and DOE.
D. Operating and Other Expenses ($50,000/year). A budget for routine office
expenses, the maintenance of the PMBB Distinguished Seminar Series (which brings 34 highly visible scientists to the Columbus and Wooster campuses per year), expenses
relative to outreach and engagement, video-linking, web-site maintenance, faculty and
student recruitment, and other miscellaneous activities is requested ($50,000/year).
6
BIOGRAPHICAL SKETCH – PIERLUIGI BONELLO
A.
Appointments
2005-present
2000-2005
B.
Associate Professor, Department of Plant Pathology, Ohio State University, Columbus, OH
Assistant Professor, Department of Plant Pathology, Ohio State University, Columbus, OH
Professional Preparation
University of California, Davis
Host-Pathogen-Insect
Interactions
Post-doc, 1997-2000
Host-Pathogen-Insect
Interactions;
Mycorrhizal
Ecology
Post-doc, 1994-1996
GSF - Forschungszentrum für
Umwelt und Gesundheit
Munich, Germany
Air pollution and
Disease Resistance
Post-doc, 1991-1992
University of Oxford, U.K.
Forest Pathology
Ph.D., 1991
University of Padova, Italy
Forest Sciences
"Laurea" (=M.Sc.), 1987
University of California, Berkeley
C.
Relevant Publications – Last Four Years
Barto, E.K., S. Enright, A. Eyles, C.M. Wallis, R. Chorbadjian, R. Hansen, D.A. Herms, P. Bonello and D.F.
Cipollini. 2008. Effects of soil fertility on systemic protein defense responses of austrian pine to attack by a
fungal pathogen and an insect defoliator. Journal of Chemical Ecology (in press).
Bonello, P., N. Luchi, P. Capretti, and M. Michelozzi. 2008. Host-mediated effects of Heterobasidion annosum
s.s. infection on severity of Diplodia pinea tip blight in Italian stone pine (Pinus pinea L.). Tree Physiology
28, 1653–1660.
Wallis, C.M., Eyles, A., Chorbadjian, R., McSpadden-Gardner, B.B., Hansen, R., Cipollini, D.F., Herms, D.A.
and P. Bonello. 2008. Systemic induction of phloem secondary metabolism and its relationship to resistance
to a canker pathogen in Austrian pine. New Phytologist 177, 767–778.
Luchi, N., P. Capretti, and P. Bonello. 2007. Production of Diplodia scrobiculata and Diplodia pinea pycnidia on
ground Austrian pine needle agar medium. Phytopathologia Mediterranea 46, 230-235.
Eyles, A., R. Chorbadjian, C.M. Wallis, R.C. Hansen, D.F. Cipollini, D.A. Herms, and P. Bonello. 2007. Crossinduction of systemic induced resistance between an insect and a fungal pathogen in Austrian pine over a
fertility gradient. Oecologia 153: 365-374.
Whitehill, J., J. S. Lehman, and P. Bonello. 2007. Ips pini (Curculionidae: Scolytinae) is a vector of the fungal
pathogen, Sphaeropsis sapinea (Coelomycetes), to Austrian pines, Pinus nigra (Pinaceae). Environmental
Entomology 36:114-120.
Blodgett, J. T., A. Eyles, and P. Bonello. 2007. Organ-dependent induction of systemic resistance and systemic
susceptibility in Pinus nigra inoculated with Sphaeropsis sapinea and Diplodia scrobiculata. Tree Physiology
27:511-517.
Bonello, P., T. R. Gordon, D. A. Herms, D. L. Wood, and N. Erbilgin. 2006. Nature and ecological implications
of pathogen-induced systemic resistance in conifers: A novel hypothesis. Physiological and Molecular Plant
Pathology 68: 95-104. (Invited review article.)
Wang, D., A. Eyles, and P. Bonello. 2006. Systemic aspects of host-pathogen interactions in Austrian pine (Pinus
nigra): a proteomics approach. Physiological and Molecular Plant Pathology 68:149-157.
Blodgett, J. T., D. A. Herms, and P. Bonello. 2005. Fertilization effects on red pine defense chemistry and
resistance to Sphaeropsis sapinea. Forest Ecology and Management 208: 373-382.
Luchi, N., R. Ma, P. Capretti, and P. Bonello. 2005. Systemic induction of traumatic resin ducts and resin flow
in Austrian pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata. Planta
221: 75-84.
D.
•
•
•
•
•
Synergistic Activities
Developed a hands-on workshop for high school teachers on “Chemical Ecology – Interactions Between
Trees and Pathogens”.
Developed a hands-on class for third graders on “My career as a tree ecologist”.
Developed two new courses at OSU: “Advanced Topics in Fungal Biology”; “Fungi in Natural
Ecosystems”, while a third was co-developed with Dr. McSpadden Gardener: “Ecology of Plant-Associated
Microbes”.
Associate Editor for Plant Disease (Jan. 2001-Dec. 2003); Editorial Board for Physiological and
Molecular Plant Pathology (2007-2010); ad hoc reviewer for Annals of Forest Science, Australasian Plant
Pathology, Canadian Journal of Botany, Canadian Journal of Forest Research, Forest Ecology and
Management, Forest Pathology, Forest Science, Journal of Chemical Ecology, Journal of Experimental
Botany, Naturwissenschaften, Molecular Plant Pathology, Mycologia, Mycological Research,
Mycorrhiza, Oecologia, Phytoparasitica, Phytopathology, Physiological and Molecular Plant Pathology,
Planta, Plant Health Progress, Scandinavian Journal of Forest Research, Theoretical and Applied
Genetics, Tree Physiology, Tropical Agriculture.
Official Ohio representative to Multistate Research Projects: W1187, “Interactions Among Bark Beetles,
Pathogens, and Conifers in North American Forests” (immediate past chair), and NCR193, “IPM
Strategies for Arthropod Pests and Diseases in Nurseries and Landscapes”.
E.
Collaborators & Other Affiliations
(i) Collaborators and Co-Editors (last 48 months):
J.T. Blodgett – US Forest Service; M.J. Boehm - Ohio State University; P. Capretti – University of Florence,
Italy; D. Cipollini – Wright State University; N. Erbilgin – University of California, Berkeley; A. Eyles – CSIRO;
M. Garbelotto - University of California, Berkeley; T.R. Gordon – University of California, Davis; T.L. Graham Ohio State University; R.C. Hansen - Ohio State University; D.A Herms - Ohio State University; D. Karnoski Michigan Technological University; N. Kleczewski - Ohio State University; J.H. LaForest – Univ. of Georgia,
Tifton, GA; C. Liang – Miami University; P.E. Lipps - Ohio State University; N. Luchi – University of Florence,
Italy; R. Ma – unattached; W. Mattson – US Forest Service; B. McPherson – University of California, Berkeley;
B.B. McSpadden Gardener – Ohio State University; M. Michelozzi, CNR, Florence, Italy; M. Mielke – US Forest
Service; A. Nagle - Ohio State University; F. Ockels – Water Quality Dept., City of Dallas, TX; K. Raffa –
University of Wisconsin, Madison; E. Rebek – Oklahoma State University; D.M. Rizzo – University of California,
Davis; D. Smitley - Michigan State University; P. Svihra – private consultant; N. Taylor - Ohio State University;
S.L. Thomas – California Dept. of Food and Agriculture; C. Wallis – University of Northern British Columbia; J.
Whitehill - Ohio State University; D.L. Wood – University of California, Berkeley.
(ii) Graduate and Postdoctoral Advisors:
R.B. Pearce (deceased) - University of Oxford, UK, Ph.D. advisor; W. Heller, GSF - Forschungszentrum für
Umwelt und Gesundheit, Munich, Germany, postdoctoral sponsor; T.D. Bruns - University of California, Berkeley,
postdoctoral sponsor; T.R. Gordon - University of California, Davis, postdoctoral sponsor; D.L. Wood - University
of California, Berkeley, postdoctoral sponsor.
(iii) Thesis Advisor and Postgraduate-Scholar Sponsor:
Post-docs
A. Eyles (completed); J.T. Blodgett (completed).
Graduate Students
Annemarie Nagle – M.S. (current); Justin Whitehill – Ph.D. (current); Nathan Kleczewski - Ph.D. (current); Brian
Goldberger – Ph.D. (current); Christopher Wallis - Ph.D. (completed); France Ockels – M.S. (completed); Joe
LaForest – M.S. (completed); Rui Ma – M.S. (completed).
Ding, Biao
Principal Investigator/Program Director (Last, First, Middle):
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Biao Ding
Professor of Plant Cellular and Molecular Biology
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
DEGREE
(if applicable)
YEAR(s)
B.S.
M.S.
Ph.D.
Postdoc
1982
1986
1991
1991-1994
Beijing Forestry University
Cornell University
Cornell University
University of California, Davis
FIELD OF STUDY
Forestry
Plant Anatomy
Plant Cell Biology
Plant Cell Biology
A. Positions and Honors
Professional Positions
1994-1999
Assistant Professor, Department of Botany, Oklahoma State University, OK
1999-2000
Associate Professor, Department of Botany, Oklahoma State University, OK
2000-2005
Associate Professor, Department of Plant Cellular and Molecular Biology and Plant
Biotechnology Center, Ohio State University, OH
2005Professor, Department of Plant Cellular and Molecular Biology and Plant Biotechnology
Center, Ohio State University, OH
Other Experience and Professional Memberships
Member, American Society for Virology
Member, American Society for Microbiology
Member, International Society of Molecular Plant-Microbe Interactions
Member, American Society of Plant Biologists
Honors
1983-1985 Graduate Fellowship for Overseas Studies, the Chinese Government
1999
The College of Arts and Sciences Faculty Council Junior Faculty Award for Scholarly
Excellence, Oklahoma State University
2005
Harlan Hatcher Memorial Award for Excellence, Colleges of Arts and Sciences, Ohio State
University (2005)
2005-2007 Outstanding Overseas Youth Research Award, National Science Foundation of China
B. Selected Peer-Reviewed Publications
(Selected from 42 peer-reviewed research papers)
1. Ding, B., Haudenshield, J.S., Hull, R.J., Wolf, S., Beachy, R.N., and Lucas, W.J. (1992). Secondary
plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in
transgenic tobacco plants. Plant Cell 4:915-928.
PHS 398/2590 (Rev. 05/01)
Page
5
Biographical Sketch Format Page
Principal Investigator/Program Director (Last, First, Middle):
2. Ding, B., Haudenshield, J.S., Willmitzer, L., and Lucas, W.J. (1993). Correlation between arrested
secondary plasmodesmal development and onset of accelerated leaf senescence in yeast invertase
transgenic tobacco plants. Plant J. 4:179-189.
3. Fujiwara, T., Giesmann-Cookmeyer, D., Ding, B., Lommel, S.A., and Lucas, W.J. (1993). Cell-to-cell
trafficking of macromolecules through plasmodesmata potentiated by the red clover necrotic mosaic virus
movement protein. Plant Cell 5:1783-1794.
4. Sanger, M., Passmore, B., Falk, B.W., Bruening, G., Ding, B., and Lucas, W.J. (1994). Symptom
severity of beet western yellows virus strain ST9 is conferred by the ST9-associated RNA and is not
associated with virus release from the phloem. Virology 200:48-55.
5. Lucas, W.J., Bouche-Pillon, S., Jackson, D.P., Nguyen, L., Baker, L., Ding, B. and Hake, S. (1995).
Selective trafficking of KNOTTED1 homeodomain protein and its mRNA through plasmodesmata.
Science 270:1980-1983.
6. Ding, B., Li, Q-b., Nguyen, L., Palukaitis, P., and Lucas, W.J. (1995). Cucumber mosaic virus 3a protein
potentiates cell-to-cell trafficking of CMV-vRNA in tobacco plants. Virology 207:345-353.
7. Nguyen, L., Lucas, W. J., Ding, B. and Zaitlin, M. (1996). Viral trafficking is inhibited in replicasemediated resistant transgenic tobacco plants. Proc. Natl. Acad. Sci. (U.S.A.) 93:12643-12647.
8. Ding, B., Kwon, M.-O., and Warnberg, L. (1996). Evidence that actin filaments are involved in controlling
the permeability of plasmodesmata in tobacco mesophyll. Plant J. 10:157-164.
9. Itaya, A., Hickman, H., Bao, Y., Nelson, R. and Ding, B. (1997). Cell-to-cell trafficking of cucumber
mosaic virus movement protein:green fluorescent protein fusion produced by biolistic gene
bombardment in tobacco. Plant J. 12:1223-1230.
10. Ding, B., Kwon, M.-O., Hammond, R. and Owens, R. (1997). Cell-to-cell movement of potato spindle
tuber viroid. Plant J. 12:931-936.
11. Itaya, A., Woo, Y.-M., Masuta, C., Bao, Y., Nelson, R., and Ding, B. (1998). Developmental regulation of
intercellular protein trafficking through plasmodesmata in tobacco leaf epidermis. Plant Physiol.
118:373-385.
12. Woo, Y.-M., Itaya, A., Owens, R.A., Tang, L., Hammond, R.W., Chou, H.-C., Lai, M.M.C., and Ding, B.
(1999). Characterization of nuclear import of potato spindle tuber viroid RNA in permeabilized
protoplasts. Plant J. 17:627-635.
13. Itaya, A., Liang, G., Nelson, R.S., and Ding, B. (2000). Nonspecific intercellular protein trafficking probed
by green fluorescent protein. Protoplasma 213:165-175.
14. Zhu, Y., Green, L., Woo, Y.-M., Owens, R., and Ding, B. (2001). Cellular basis of Potato spindle tuber
viroid systemic movement. Virology 279:69-77.
15. Itaya, A., Folimonov, A., Matsuda, Y., Nelson, R., and Ding, B. (2001). Potato spindle tuber viroid as
inducer of RNA silencing in infected tomato. Molec. Plant-Microbe Interact. 14:1332-1334.
16. Owens, R.A., Blackburn, M. and Ding, B. (2001). Possible involvement of phloem protein 2 in long
distance viroid movement. Molec. Plant-Microbe Interact. 14:905-909.
17. Qi, Y. and Ding, B. (2002) Replication of Potato spindle tuber viroid in cultured cells of tobacco and
Nicotiana benthamiana: The role of specific nucleotides in determining replication levels for host
adaptation. Virology 302:445-456.
18. Itaya, A., Ma, F., Qi, Y., Matsuda, Y., Zhu, Y., Liang, G., and Ding, B. (2002). Plasmodesma-mediated
selective protein traffic between “symplasmically-isolated” cells probed by a viral movement protein.
Plant Cell 14: 2071-2083.
19. Zhu, Y., Qi, Y., Xun, Y., Owens, R., and Ding, B. (2002) Movement of Potato spindle tuber viroid reveals
regulatory points of phloem-mediated RNA traffic. Plant Physiol. 130:138-146.
20. Itaya, A., Matsuda, Y., Gonzales, R. A., Nelson, R.S., and Ding, B. (2002) Potato spindle tuber viroid
strains of different pathogenicity induces and suppresses expression of common and unique genes in
infected tomato. Molec. Plant-Microbe Interact. 15:990-999.
PHS 398/2590 (Rev. 05/01)
Page
Continuation Format Page
Principal Investigator/Program Director (Last, First, Middle):
21. Matsuda, Y., Liang, G., Zhu, Y., Ma, F., Nelson, R.S., and Ding, B. (2002). The Commelina yellow mottle
virus promoter drives companion cell-specific gene expression in multiple organs of transgenic tobacco.
Protoplasma 220:51-58.
22. Qi, Y. and Ding, B. (2003). Differential subnuclear localization of RNA strands of opposite polarity
derived from an autonomously-replicating viroid. Plant Cell 15:2566-2577.
23. Qi, Y. and Ding, B. (2003). Inhibition of cell growth and shoot development by a specific nucleotide
sequence in a noncoding viroid RNA. Plant Cell 15:1360-1374.
24. Qi, Y., Pélissier, T., Itaya, A., Hunt, E., Wassenegger, M., and Ding, B. (2004). Direct role of a viroid
RNA motif in mediating directional RNA trafficking across a specific cellular boundary. Plant Cell
16:1741-1752.
25. Qi, Y., Zhong, X., Itaya, A., and Ding, B. (2004). Dissecting RNA silencing in protoplasts uncovers novel
effects of viral suppressors on the silencing pathway at the cellular level. Nucleic Acids Research 32
(22): e179.
26. Cao, X., Zhou, P., Zhang, X., Zhu, S., Zhong, X., Xiao, Q., Ding, B., Li, Y. (2005). Identification of an RNA
silencing suppressor from a plant double-stranded RNA virus. J. Virol. 79:13018-13027. (Selected by
Editor for Spotlight)
27. Zhong, X., Leontis, N. B., Qian, S., Itaya, A., Boris-Lawrie, K., and Ding, B. (2006). Tertiary structural and
functional analyses of a viroid RNA motif by isostericity matrix and mutagenesis reveal its essential role in
replication. J. Virol. 80:8566-8581.
28. Itaya, A., Zhong, X., Bundschuh, R., Qi, Y., Wang, Y., Takeda, R., Harris, A.R., Molina, C., Nelson, R.S.,
and Ding, B. (2007). A structured viroid RNA is substrate for dicer-like cleavage to produce biologically
active small RNAs but is resistant to RISC-mediated degradation. J. Virol. 81:2980-2994 (Highlighted by
Faculty 1000 Biology as “Must Read” on April 16, 2007)
29. Wang, Y., Zhong, X., Itaya, A., and Ding, B. (2007). Evidence for the in vivo existence of loop E motif of
potato spindle tuber viroid. J. Virol. 81:2074-2077.
30. Zhong, X., Tao, X., Stombaugh, J., Leontis, N., and Ding, B. (2007). Tertiary structure and function of an
RNA motif required for plant vascular entry to initiate systemic trafficking. EMBO J. 26:3836-3846.
31. Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y. (2007) The P2 capsid
protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells. Proc.
Natl. Acad. Sci. USA 104:19547-19552.
32. Itaya, A., Bundschuh, R., Archual, A., Joung, J.-G., Fei, Z., Dai, X., Zhao, P., Tang, Y., Nelson, R.S. and
Ding, B. (2007). Small RNAs in tomato fruit and leaf development. Biochim Biophy Acta 1779:99-107.
33. Zhong, X., Archual, A.J., Amin, A.A., and Ding, B. (2008) A genomic map of viroid RNA motifs critical for
replication and systemic trafficking. Plant Cell 20:35-47. (Highlighted as Editor’s Choice in the February
8, 2008 issue of Science.)
C. Research Support
Ongoing Research Support
NSF IBN-0238412 Ding (PI)
8/1/2006-7/31/2009
An integrative approach to elucidate RNA replication and systemic trafficking
This study investigates the RNA structural motifs and cellular factors critical for replication as well as RNA
structural motifs essential for intercellular trafficking, using potato spindle tuber viroid as the model.
Role: PI
Completed Research Support
USDA NRICGP 2004-35304-15005 Ding (PI)
Biochemical and genetic analyses of plant microRNA biogenesis
This project investigates how plant microRNAs are produced.
Role: PI
PHS 398/2590 (Rev. 05/01)
Page
9/1/2004-8/31/2008
Continuation Format Page
Principal Investigator/Program Director (Last, First, Middle):
NSF IBN-0238412 Ding (PI)
3/15/2003-3/14/2006
Mechanisms of phloem-mediated RNA traffic
This study investigates mechanisms that control phloem-mediated long-distance RNA traffic, using potato
spindle tuber viroid as the model.
Role: PI
NSF IOB-0515745 Ding (PI)
3/1/2005-2/28/2007
Novel biogenesis and function of small RNAs derived from a plant pathogen
This project studies the biogenesis and function of viroid-derived small RNAs.
Role: PI
USDA NRICGP 2002-35304-12272 Ding (PI)
8/15/2002-8/14/2004
Cellular and genetic analysis of intercellular protein traffic
The goal of this study was to investigate cellular boundaries that regulate intercellular protein trafficking and
develop genetic screening systems of Arabidopsis mutants defective in protein trafficking.
Role: PI
The Samuel Roberts Noble Foundation Ding (PI) Nelson (CoPI) 1/1/2000-11/1/2003
Plant development and viral movement
This project examined how plant development affects viral infection patterns.
Role: PI
The Ohio Plant Biotechnology Consortium Ding (PI)
7/1/2004-6/30/2005
Development of novel microRNAs for gene function studies and for viral resistance
This projects tests the idea of engineering vectors to express designer microRNAs for gene regulation and
anti-viral infection.
Role: PI
USDA NRICGP 2001-35304-09928 Ding (PI)
12/1/2000-11/30/2002
Intercellular protein trafficking and leaf development
This project investigated the role of leaf developmental stages in controlling intercellular protein trafficking
patterns.
Role: PI
PHS 398/2590 (Rev. 05/01)
Page
Continuation Format Page
JOHN J. FINER
Department of Horticulture and Crop Science, Plant Molecular Biology and
Biotechnology Program, Ohio Agricultural Research and Development
Center, The Ohio State University, 1680 Madison Ave, Wooster, Ohio 44691,
USA, http://www.oardc.ohio-state.edu/plantranslab/
Tel: 330-263-3880, Fax: 330-263-3887, E-mail - [email protected]
PROFESSIONAL PREPARATION
Miami University, Oxford, OH
Texas A&M University
Texas A&M University
Postdoctoral Fellow
Botany
Plant Physiology
Plant Physiology
CIBA-GEIGY Biotechnology
BS 1978
MS 1981
PhD 1984
1984-1986
APPOINTMENTS
1999-present
1991-1999
1986-1991
Professor, Department of Horticulture and Crop Science, Plant Molecular
Biology and Biotechnology Program, The Ohio State University
Associate Professor, Department of Horticulture and Crop Science, The
Ohio State University
Assistant Professor, Department of Agronomy, The Ohio State
University
PUBLICATIONS
Chiera JM, Lindbo JA, Finer JJ (in press) Quantification and extension of transient GFP
expression by the co-introduction of a suppressor of silencing. Transgenic Research, DOI:
10.1007/s11248-008-9192-5
Wu C, Chiera JM, Ling PP, Finer JJ (in press) Isoxaflutole treatment leads to reversible tissue
bleaching and allows for more effective detection of GFP in transgenic soybean tissues. In
Vitro Cellular and Developmental Biology – Plant, DOI: 10.1007/s11627-008-9126-1
Finer JJ, Larkin KM (in press) In (ed. PB Kirti) Handbook of New Technologies for Genetic
Improvement of Legumes, “Genetic transformation of soybean using particle bombardment
and SAAT approaches” Haworth Press, Binghamton, New York
Finer JJ, Dhillon T (2008) Transgenic Plant Production, pp 245-272 In: Plant Biotechnology and
Genetics: Principles, Techniques and Applications, ed. C. Neal Stewart, Jr. Wiley and Sons,
New York
Finer JE, Finer JJ (2007) A simple method for reducing moisture condensation on Petri dish lids.
Plant Cell Tissue and Organ Culture 91:299-304
Chiera JM, Bouchard RA, Dorsey SL, Park EH, Buenrostro-Nava MT, Ling PP, Finer JJ (2007)
Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their
characterization using a new automated image collection and analysis system. Plant Cell
Reports 26:1501-1509
Chiera JM, Streeter JG, Finer JJ (2006) Ononitol and pinitol production in transgenic soybean
containing the inositol methyl transferase gene from Mesembryanthemim crystallinum. Plant
Science 171:647–654
Buenrostro-Nava MT, Ling PP, Finer JJ (2006) Comparative analysis of 35S and Lectin
promoters in transgenic soybean tissue using an automated image acquisition system and
image analysis. Plant Cell Reports 25:920-926
Finer JJ, Beck SL, Buenrostro-Nava MT, Chi YT, Ling PP (2006) In (Eds. S Dutta Gupta, Y
Ibaraki) Plant Tissue Culture Engineering; Focus in Biotechnology, “Monitoring Gene
Expression in Plant Tissues; Using green fluorescent protein with automated image collection
and analysis” p 31-46, Springer, Dordrecht
Buenrostro-Nava MT, Ling PP, Finer JJ (2005) Development of an automated image acquisition
system for monitoring gene expression and tissue growth. Transactions of the Amer Soc for
Agric Eng 48:841-847
SYNERGISTIC ACTIVITIES
Served in the Society for In Vitro Biology, as secretary, vice-chair, chair, and development officer
of the Plant Division. Currently serving as Secretary and Member of the Executive Board of the
society.
Developed new particle bombardment instrumentation that greatly expanded the use of this
technology in smaller US laboratories and in developing countries. Developed robotics and gene
tracking system, allowing simple quantification of gene expression data. Isolation and
characterization of soybean promoters using automated image analysis and gfp.
Developed curriculum materials for Plant Cell Transformation (graduate) and Ethics in
Biotechnology (undergraduate) courses.
Served as an electronics expert in biotechnology with COSI (Center of Science and Industry) in
Columbus, OH for 7 years. COSI is a science center for K-12 students.
Developed a seed germination exercise kit (Adolescent Seeds; Embryo Germination Before their
Time) for middle and high school science students.
COLLABORATORS AND OTHER AFFILIATIONS
i. Collaborators
Randy Dinkins (USDA/ARS), Elisabeth Grabau (Virginia Tech), Steve Knapp (University of
Georgia), John Lindbo (University of California, Davis), EuiHo Park (Yeungnam University,
Korea), Wayne Parrott (Univ of Georgia), Paul Rushton (University of Virginia), Harold Trick
(Kansas State Univ), Lila Vodkin (Univ of Illinois), Jack Widholm (Univ of Illinois), Adriana
Rodriguez (University of Sao Paulo), Mike Timko (University of Virginia).
ii. Graduate and Postdoctoral Advisors
Graduate Advisor: Roberta Smith, Texas A&M University (retired)
Postdoctoral Advisor: Mary-Dell Chilton (Syngenta)
iii. Thesis and Postgraduate-Scholar Sponsor
Robert Bouchard (The Ohio State University), Aubry Brzozowski (BallHelix), Marco
Buenrostro-Nava (Texas A&M University), Tai-Sheng Cheng (Tainan Teachers College,
Tainan, Taiwan), Taniya Dhillon (The Ohio State University), Summer Dorsey (DuPont
Agriculture and Nutrition), Masood Hadi (Laurence Livermore Research Labs), Carlos
Hernandez-Garcia (The Ohio State University), Kathryn Larkin (Arizona State University),
Marcelo Pomeranz (The Ohio State University), Eliane Santarem (Pontifícia Universidade
Católica do Rio Grande do Sul), Harold Trick (Kansas State University), Sanchita
Vaghchhipawala (Noble Foundation), Philippe Vain (John Innes Research Center, UK), Nicole
Waterland (The Ohio State University), Congling Wu (University of Georgia)
Total number of graduate students advised: 11
Total number of postdoctoral scholars advised: 10
David M. Francis
Department of Horticulture and Crop Science, The Ohio State University-Ohio Agricultural Research
and Development Center, Wooster, OH 44691
Tel: (330) 263-3893; FAX: 330-263-3887; E-mail: [email protected]
Professional Preparation
Pomona College, Claremont, CA
University of California, Davis, CA
University of California, Davis, CA
B.A.
Ph.D
Post-Doc
1984
1991
1991-1995
Biology
Genetics
Genetics
Appointments
(2003-present) Associate Professor, Department of Horticulture and Crop Science, OARDC/The Ohio
State University, Wooster OH 44691.
(1999-2003)
Asistant Professor, Department of Horticulture and Crop Science, OARDC/The Ohio
State University, Wooster OH 44691.
(1995-1999)
Research Scientist and Adjunct Assistant Professor, Department of Horticulture and Crop
Science, OARDC/The Ohio State University, Wooster OH 44691.
(1991-1999)
Post-doctoral associate at the Department of Vegetable Crops, University of California,
Davis, CA 95616.
(1985-1991)
McKnight Foundation Fellowship for interdisciplinary studies in plant/pathogen
interactions, Univ. of CA, Davis, CA 95616.
Publications (last five years)
Bentley, S. D., C. Corton, A. Barron, L. Clark, J. Doggett, B. Harris, D. Ormond, M. A. Quail, S. E.
Brown, D. Knudson, D. Francis, J. Parkhill, C. Ishimaru. 2008. Genome of the actinomycete
plant pathogen Clavibacter michiganensis subspecies sepedonicus suggests recent niche
adaptation. J. Bact. 190:2150-2160
Darrigues, A., J. Hall, N. Dujmovic, S. Gray, E. van der Knaap, and D. M. Francis. 2008. Tomato
Analyzer – Color Test: a new tool for efficient digital phenotyping. J. Amer. Soc. Hort. Sci.
133(4):579–586.
Darrigues, A., S. Schwartz, D. M. Francis. 2007. Optimizing sampling of tomato fruit for carotenoid
content with application to assessing the impact of ripening disorders. Journal of Agricultural
and Food Chemistry 56: 483–487
Van Deynze, A., K. Stoffel, C. R. Buell, A. Kozik, J. Liu, E. van der Knaap, D. M. Francis. 2007.
Diversity in conserved genes in tomato. BMC Genomics 8:465.
http://www.biomedcentral.com/1471-2164/8/465
Unlu, N. Z., T. Bohn, D. Francis, S. J. Clinton, S. J. Schwartz. 2007. Carotenoid Absorption in Humans
Consuming Pasta Sauces Obtained from Tangerine or High-B-Carotene Varieties of Tomatoes.
Journal of Agricultural and Food Chemistry. 55(4):1597-1603.
Unlu, N. Z., Bohn, T., Francis, D. M., Nagaraja, H. N., Clinton, S. K., and Schwartz, S. J. 2007.
Lycopene from heat-induced cis-isomer-rich tomato sauce is more bioavailable than from alltrans-rich tomato sauce in human subjects. Br. J. Nutr. 98: 140-146
Halim, Y., S. Schwartz, D. Francis, N. A. Baldauf, and L. Rodriguez-Saona. 2006. Direct Determination
of Lycopene Content in Tomatoes (Lycopersicon esculentum) by Attenuated Total Reflectance
Infrared (ATR-IR) Spectroscopy and Multivariate Analysis. Journal of AOAC International.
89:1257-1262.
Yang, W. and D. M. Francis. 2005. Marker Assisted Selection for Combining Resistance to Bacterial
Spot and Bacterial Speck in Tomato. J. Amer. Soc. Hort. Sci. 130:716-721.
Stommel, John R. Judith A. Abbott, T. Austin Campbell, and David Francis 2005. Inheritance of Elastic
and Viscoelastic Components of Tomato Firmness Derived from Intra- and Interspecific Genetic
Backgrounds J. Amer. Soc. Hort. Sci. 130: 598-604.
Yang, W., E. J. Sacks, M. L. Lewis Ivey, S. A. Miller, and D. M. Francis. 2005. Resistance in
Lycopersicon esculentum intraspecific crosses to Race T1 strains of Xanthomonas campestris pv.
vesicatoria causing bacterial spot of tomato. Phytopathology 95:519-527.
Francis, D. M. and S. A. Miller. 2005. Ohio 9834 and Ohio 9816: processing tomato breeding lines with
partial resistance to race T1 of bacterial spot. HortScience 40: 1566-1568.
Hackett, M.M., Lee, J.H., Francis, D. and Schwartz, S. 2004. Thermal stability and isomerization of
lycopene in tomato oleoresins from different varieties. J. Food Sci., 69(7) 536-541.
Coaker G., B. Willard, M. Kinter, E. J. Stockinger, and D. Francis. 2004. Proteomic analysis of resistance
to bacterial canker of tomato. Molecular Plant Microbe Interactions. 17:1019-1028.
Coaker G., and D. Francis. 2004. Mapping, genetic effects, and epistatic interaction of two bacterial
canker resistance QTLs from Lycopersicon hirsutum. Theor Appl Genet. 108:1047-1055
Yang, W., X. Bai, E. Kabelka, C. Eaton, S. Kamoun, E. van der Knaap, and D. Francis. 2004. Discovery
of single nucleotide polymorphisms in Lycopersicon esculentum by computer aided analysis of
expressed sequence tags. Molecular Breeding. 14: 21-34.
Kabelka, E., W. Yang, and D. M. Francis. 2004. Improved Tomato Fruit Color within an Inbred
Backcross Line Derived from Lycopersicon esculentum and L. hirsutum Involves the Interaction
of Loci. J. Amer. Soc. Hort. Sci. 129:250-257
Huitema, E., V. G. A. A. Vleeshouwers, D.M. Francis and S.Kamoun. 2003. Active defense responses
associated with nonhost resistance of Arabidopsis thaliana to the oomycete pathogen
Phytophthora infestans. Molecular Plant Pathology. 4: 487-500.
Scott, J. W., D. M. Francis, S. A. Miller, G. C. Somodi, J. B. Jones. 2003. Tomato bacterial spot
resistance derived from PI 114490; inheritance to race T2 and relationship across three pathogen
races. J. Amer. Soc. Hort. Sci. 128:698-703.
Qu, S., G. Coaker, D. Francis, B. Zhou, and G.L. Wang. 2003. Development of a new transformationcompetent artificial chromosome (TAC) vector and construction of tomato and rice TAC
libraries. Molecular Breeding. 12: 297-308.
Burnham, K.D., A.E. Dorrance, D.M. Francis, R.J. Fioritto, and S.K. St. Martin. 2003. Rps8, A New
Locus in Soybean for Resistance to Phytophthora sojae Crop Science 43: 101-105.
Huitema, E., V. G. A. A. Vleeshouwers, D.M. Francis and S.Kamoun. 2003. Active defense responses
associated with nonhost resistance of Arabidopsis thaliana to the oomycete pathogen
Phytophthora infestans. Molecular Plant Pathology. 4: 487-500.
Synergistic Activities
Member of the National Plant Germplasm System (NPGS) Crop Germplasm Committee (CGC) for
tomato (2001-Present). Chair of the NPGS CGC for tomato (2005-present).
Ohio Representative to the United States Department of Agriculture (USDA) North Central Region 7
(NC7) Germplasm Committee (2000-present). Secretary for (NC7) Germplasm Committee
(2002-2004).
Panel Member, joint USDA-DOE feedstock genomics, Rockville, MD, April 2006.
Member of the International Scientific Committee for the 8 th International Symposium on the Processing
Tomato and the 5th World Congress on the Processing Tomato (2002), Istanbul, Turkey; member
of the International Scientific Committee for the 9 th International Symposium on the Processing
Tomato and the 6th World Congress on the Processing Tomato (2004), Melbourne, Austalia.
Co-Coordinator of the common use DNA Marker Laboratory in the Department of Horticulture and Crop
Science; Molecular and Cellular Imaging Center Advisory Committee, OSU-OARDC, Wooster
OH 44691; 1999-present.
Graduate and Postdoctoral Advisors
R. W. Michelmore (Ph.D advisor, Univ. of California, Davis)
D. A. St. Clair (Post-doctoral advisor, Univ. of California, Davis)
Thesis Advisor and Postgraduate-Scholar Sponsor
Thesis advisor for:
Audrey Darrigues, PhD student (2004-present)
Susana de Jesus, MS 2005, Natural Products Chemist, Instituto de Inovacion en Biotecnologia e Industria
(IIBI), Santo Domingo, Dominican Republic
Gitta Coaker, PhD 2003, Currently Assistant Professor, University of California, Dept. of Plant
Pathology, Davis, CA.
Eileen Kabelka, Graduate Student, PhD 2001, Currently Ass. Prof. University of Florida, Gainsville, FL.
Postgraduate advisor for:
Matt Robbins, Post-doctoral researcher (2006-present).
Sung-Chur Sim, Post-doctoral researcher (2006-present).
Alba McIntyre, Post-doctoral researcher (2002-2006). Currently Assistant Professor, Wayne College,
University of Akron, OH.
Wencai Yang, Post-doctoral researcher (2001-2005). Currently Assistant Professor, Dept. of Vegetable
Science, China Agricultural University, Bejing, China.
Kara Burnham, Post-doctoral researcher (1999-2002). Currently Assistant Professor, Dept. of Biology,
Pacific University, Forest Grove, Oregon.
Frank Dailey, Post-doctoral researcher (2002). Currently Assistant Professor, Microbiology and Medical
Pathology, Lake Erie College School of Pharmacy, Erie, PA.
Tea Meulia, Post-doctoral researcher (1999). Currently Department Head of the Molecular and Cellular
Imaging Center, Wooster, OH.
Erik Sacks, Post-doctoral researcher (1997-1998). Currently Research Geneticist, USDA, Stoneville, MS.
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Venkat Gopalan
Associate Professor of Biochemistry
eRA COMMONS USER NAME
GOPALAN05
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
University of Madras, Madras, INDIA
University of New Mexico, Albuquerque, NM
Yale University, New Haven, CT
DEGREE
(if applicable)
B.Sc.
Ph.D.
Postdoc.
YEAR(s)
1986
1991
1992-1997
FIELD OF STUDY
Chemistry
Biochemistry
Molecular biology
A. Positions and Honors
Academic Appointments
1986-1991
Doctoral studies with Robert H. Glew, Ph.D., Dept. of Biochemistry, University of Pittsburgh, Pittsburgh, PA and University of New Mexico, Albuquerque, NM
1992-1997
Postdoctoral training with Sidney Altman, Ph.D., Dept. of Molecular, Cellular and
Developmental Biology, Yale University, New Haven, CT
1998-2003
Assistant Professor, Dept. of Biochemistry, The Ohio State University, Columbus, OH
2000-present
Adjunct Faculty, Dept. of Plant Biology, The Ohio State University, Columbus, OH
2003-present
Associate Professor, Dept. of Biochemistry, The Ohio State University, Columbus, OH
Selected Professional Activities
1998-present
Reviewer: BBA, Biochemistry, Genome Biology, Journal of Biological Chemistry, Journal
of Molecular Biology, Molecular BioSystems, Molecular Microbiology, Mutation Research,
Nucleic Acids Research, Oligonucleotides, PNAS, RNA, and Science.
1999-present
Ad hoc grant reviewer for: Petroleum Research Fund, Wellcome Trust, and NSF
2003-2004
Panel Member, Biochemistry of Gene Expression Panel, NSF
Honors
1993-1995
1995-1996
2003
2005
2007
2008
Post-doctoral Fellow of the Donaghue Medical Research Foundation
Post-doctoral Fellow of the Anna Fuller Cancer Research Fund
OSU College of Biological Sciences Dean’s Award for Undergraduate Research Mentoring
OSU College of Biological Sciences Dean’s Award for Classroom Teaching
OSU Department of Biochemistry Award for Excellence
OSU Harlan Hatcher Memorial Award for Academic Excellence
B. Selected Publications (of 40)
Gopalan, V., Daniels, L. B., Glew, R. H., and Claeyssens, M. (1989) Kinetic analysis of the interaction of akyl
glycosides with two mammalian beta-glucosidases. Biochem. J. 262, 541-548.
Glew, R. H., Gopalan, V., Hubbell, C. A., Beutler, E., Geil, J. D., and Lee, R. E. (1991) A case of nonneurologic Gaucher's disease that resembles the neurologic type biochemically. J. Neuropathol. Exp. Neurol. 50, 108-117
Gopalan, V., Vander Jagt, D. J., Libell, D. P., and Glew, R. H. (1992) Transglucosylation as a probe of the
mechanism of action of mammalian cytosolic beta-glucosidase. J. Biol. Chem. 267, 9629-9638.
Gopalan, V., Pastuszyn, A., Galey, W. R., Jr., and Glew, R. H. (1992) Exolytic hydrolysis of toxic plant glucosides by guinea pig liver cytosolic beta-glucosidase. J. Biol. Chem. 267, 14027-14032.
Gopalan, V., Baxevanis, A., Landsman, D. and Altman, S. (1997) Analysis of the functional role of conserved residues in the protein subunit of ribonuclease P from Escherichia coli. J. Mol. Biol. 267, 818-829.
Gopalan, V., Golbik, R., Schreiber, G., Fersht, A. and Altman, S. (1997) Fluorescence properties of a tryptophan residue in an aromatic core of the protein subunit of ribonuclease P from Escherichia coli. J. Mol.
Biol. 267, 765-769.
Gopalan, V., Kuhne, H., Biswas, R., Li, H., Brudvig, G. W. and Altman, S. (1999) Mapping RNA-protein interactions in ribonuclease P from Escherichia coli using electron paramagnetic resonance spectroscopy.
Biochemistry 38, 1705-1714.
Biswas, R., Ledman, D., Fox, R. O., Altman, S. and Gopalan, V. (2000) Mapping RNA-protein interactions in
ribonuclease P from Escherichia coli using disulfide-linked EDTA-Fe. J. Mol. Biol. 296, 19-31.
Stephen Raj, M. L., Pulukkunat, D. K., Reckard, J. F., Thomas, G. and Gopalan, V. (2001) Cleavage of bipartite substrates by rice and maize RNase P: Application to targeted degradation of mRNAs in plants.
Plant Physiol. 125, 1187-1190.
Biswas, R., Kuhne, H., Brudvig, G. and Gopalan, V. (2001) Use of EPR spectroscopy to study macromolecular structure and function. Sci. Prog. 84, 45-67.
Wu, C-W., Eder, P. S., Gopalan, V. and Behrman, E. J. (2001) Kinetics of coupling reactions that generate
monothiophosphate disulfides: Implications for modification of RNAs. Bioconj. Chem. 12, 842-844.
Gopalan, V., Vioque, A. and Altman, S. (2002) RNase P: variations and uses. J. Biol. Chem. 277, 67596762.
Eubank, T., Biswas, R., Jovanovic, M., Litovchick, A., Lapidot, A. and Gopalan, V. (2002) Inhibition of bacterial RNase P by modified aminoglycosides. FEBS Lett. 511, 107-112.
Jovanovic, M., Sanchez, R., Altman, S. and Gopalan, V. (2002) Elucidation of structure-function relationships in the protein subunit of bacterial RNase P using a genetic complementation approach. Nucleic Acids Res. 30, 5065-5073.
Tsai, H-Y., Masquida, B., Biswas, R., Westhof, E. and Gopalan, V. (2003) Molecular modeling of the threedimensional structure of the bacterial RNase P holoenzyme. J. Mol. Biol. 325, 661-675.
Pulukkunat, D. K., Stephen Raj, M. L., Pattanayak, D., Lai, L. B. and Gopalan, V. (2003) Exploring the potential of plant RNase P as a functional genomics tool. In “Functional genomics: Methods and Protocols”
(Grotewold, E., Ed.), pp. 295-309, Humana Press, Totowa, NJ.
Eder, P. S., Hatfield, C., Vioque, A. and Gopalan, V. (2003) Bacterial RNase P as a potential target for novel
anti-infectives. Curr. Op. Invest. Drugs 4, 937-943.
Boomershine, W. P., Stephen Raj, M. L., Gopalan, V. and Foster, M. P. (2003) Preparation of uniformly labeled NMR samples in Escherichia coli under the tight control of the araBAD promoter: Expression of an
archaeal homolog of the RNase P Rpp29 protein. Prot. Expr. Purif. 28, 246-251.
Boomershine, W. P., McElroy, C. A., Tsai, H. Y., Wilson, R. C., Gopalan, V. and Foster, M. P. (2003)
Structure of Mth11/MthRpp29, an essential protein subunit of archaeal and eukaryal RNase P. Proc. Natl.
Acad. Sci. USA 100, 15398-15403.
Rangarajan, S., Raj, M. L. S., Hernandez, J. M., Grotewold, E. and Gopalan, V. (2004) RNase P-mediated
disruption of gene expression in maize cells. Biochem. J. 380, 611-616.
Behrman, E. J. and Gopalan, V. (2005) Cholesterol and plants. J. Chem. Ed. 82: 1791-1793.
Tsai, H-Y., Pulukkunat, D. K., Woznick, W. and Gopalan, V. (2006) Functional reconstitution and characterization of Pyrococcus furiosus RNase P. Proc. Natl. Acad. Sci. USA 103, 16147-16152.
Lai, L. B., Gopichandran, V., and Gopalan, V. (2006) Tangier disease: A disorder in the reverse cholesterol
transport pathway. In “Clinical Studies in Medical Biochemistry”, (Glew, R. H. and Rosenthal, M. D., Eds.),
pp. 159-166, Oxford University Press, New York, NY.
Gopalan, V. and Altman, S. (2007) Ribonuclease P: structure and catalysis. In “The RNA World” (Gesteland, R. F., Cech, T. R. & Atkins, J. F., Eds.), Electronic version, CSH Laboratory Press, New York, NY.
Gopalan, V. (2007) Uniformity amid diversity in RNase P. Proc. Natl. Acad. Sci. USA 104, 2031-2032.
Behrman, E. J. and Gopalan, V. (2007) The anomeric specificity of enzymes which act on sugars. J. Chem.
Ed. 84, 1608.
Kawamoto, S. A., Sudhahar, C. G., Hatfield, C., Sun, J., Behrman, E. J., Gopalan, V. (2008) Studies on the
mechanism of inhibition of bacterial RNase P by aminoglycosides. Nucleic Acids Res. 36, 697-704.
Pulukkunat, D. K. and Gopalan, V. (2008) Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis. Nucleic Acids Res. 36, 4172-4180.
Behrman, E. J. and Gopalan, V. (2008) Phosphoenolpyruvate: An end to handwaving. BAMBED 36, 323324.
C. Research Support
Ongoing Research Support
R01 GM067807
Foster, M. P. (PI) Gopalan, V. (Co-PI)
04/01/04-03/31/09
NIH/NIGMS
Structure and function in catalytic RNP assembly
The overall goal of this project is to use archaeal RNase P, a tRNA processing enzyme, as the model system
to gain structural insights into how proteins modulate RNA catalysis in a ribonucleoprotein complex.
Role: Co-PI
MCB-0238233
Gopalan, V. (PI)
04/01/03-09/30/08
NSF/CAREER AWARD
Characterization of plant RNase P and examination of its utility as a functional genomics tool
The goals of this project are (i) to identify and characterize the RNA and protein subunits of plant RNase P,
and (ii) assess the feasibility of RNase P-mediated inhibition of gene expression in plants.
Role: PI
Completed Research Support (partial list)
MCB-0091081
Gopalan, V. (PI)
03/01/01-08/31/04
NSF
RNA-protein interactions in bacterial ribonuclease P
Our research objectives encompassed the use of multiple biochemical and biophysical approaches to elucidate
the structural and mechanistic basis for the roles of the protein cofactor in Escherichia coli RNase P catalysis.
Role: PI
DBI-0509744
Gopalan, V. (PI)
02/01/05-07/31/07
NSF
Evaluating ribonuclease P and RNAi as tools for targeted RNA degradation in plants
The overall objective was to compare the efficacy, target specificity and spatial control of the EGS method vs.
RNAi in Arabidopsis.
Role: PI
Madge Y. (Lian-mei) Graham
Curriculum Vitae
Senior Research Scientist
Dept. of Plant Pathology
Ohio State University
Columbus, Oh 43210
Telephone: (614)-292-6828
Fax: (614)-292-4455
Email: [email protected]
I. Professional Preparation:
National Taiwan University, Agricultural Chemistry, B. S.
Purdue University, Biochemistry, Ph.D.
II. Professional Experience/Appointments:
2005-present
Senior staff research scientist, Dept. of Plant Pathology, Ohio State Univ.
2000-present
Member, Plant Molecular Biology and Biotechnology program, Ohio State Univ.
1997-2004
Research Scientist, Department of Plant Pathology, Ohio State University
1991-1996
Senior Research Associate, Dept. of Plant Pathology and Ohio Agricultural
Research and Development Center, Ohio State Univ.
1986-1990
Research Associate, Biotechnology Center and Dept. of Plant Pathology,
Ohio State Univ.
1984-1985
Research Associate, Department of Pharmacology, Washington Univ. St. Louis.
1981-1983
Research Associate, Department of Genetics, Washington Univ. St. Louis.
1978-1980
NIH Post-Doctoral Trainee, Department of Microbiology and Immunology,
Washington Univ. St. Louis
1975-1977
Post-Doctoral Researcher, Department of Pharmacology, U. of Wisconsin
1974-1975
Post-Doctoral Researcher, Department of Chemistry, Denver University
III. Research Interests:
Soybean defense regulons: induced gene expression triggered by pathogen elicitors (pathogen
associated molecular patterns or PAMPs) and chemicals causing cell death (as hypersensitive
reaction mimics). These are studied by various methods/platforms for monitoring/profiling gene
expression and via collaborative efforts of gene silencing for functional assignments in soybean.
IV. Publications:
5 selected recent papers:
Graham TL, Graham MY, Yu O. 2008. Genomics of Soybean Secondary Product Metabolism. In:
Genetics and Genomics of Soybean, Stacey G, ed. Springer Verlag, NY, pp. 241-242.
Graham TL, Graham MY, Subramanian S, Yu O. 2007. RNAi silencing of genes for elicitation or
biosynthesis of 5-deoxyisoflavonoids suppresses race specific resistance and hypersensitive cell
death in Phytophthora sojae infected tissues. Plant Physiol. 144: 728-740.
Graham MY. 2005. The Diphenylether Herbicide Lactofen Induces Cell Death and Expression of
Defense-Related Genes in Soybean. Plant Physiol. 139: 1784-1794.
Subramanian S, Graham MY, Yu O, Graham TL. 2005 RNA Interference of Soybean Isoflavone
Synthase Genes Leads to Silencing in Tissues Distal to the Transformation Site and to Enhanced
Susceptibility to Phytophthora sojae. Plant Physiol. 137:1345-1353
Graham MY, Weidner J, Wheeler K, Pelow ML, Graham TL. 2003. Pathogenesis-related protein
gene induction in soybean by wounding and the Phytophthora sojae cell wall glucan elicitor.
Physiol. Molec. Plant Pathol. 63:141-149
5 selected previous papers:
Graham TL, Graham MY. 1999. Role of hypersensitive cell death in conditioning elicitation
competency and defense potentiation. Physiol. Molec. Plant Pathol. 55:13-20.
Graham TL, Graham MY. 1996. Signaling in soybean phenylpropanoid responses-dissection of
primary, secondary, and conditioning effects of light, wounding, and elicitor treatments. Plant
Physiology 110:1123-1133.
Graham MY, Graham TL. 1994. Wound-associated Competence factors are required for the proximal
cell responses of soybean to the Phytophthora-sojae wall glucan elicitor. Plant Physiology
105:571-578.
Graham TL, Graham MY. 1991. Cellular Coordination of molecular responses in plant defense.
Molecular Plant-Microbe Interact. 4:415-422.
Olson MV, Dutchik JE Graham MY. et al. 1986. .Random-clone strategy for genomic restriction
mapping in yeast. Proc. Nat. Acad. Sci. 83:7826-7830.
V. Synergistic Activities:
a) Affiliated with IS-MPMI, APS, ASPB (also previously ASM), attend and present in 1-2 society and
other meetings/conferences annually.
b) Ad hoc reviewer for journals: Plant Physiology, Physiologia Plantarum, Plant Science,
Physiological and Molecular Plant Pathology, and Canadian Journal of Botany.
c) Ad hoc reviewer for grants from USDA and NSF.
d) Attend and present in annual meetings of Ohio Plant Biotech Consortium and involved in statewide scientific interactions. Participate in campus-wide information exchanges, particularly with
students and postdocs (via seminar series, symposia and workshops in molecular plant sciences).
These include the Plant Molecular Biology and Biotechnology (PMBB), Translational Plant
Science (TPS), Molecular Plant Pathology and other programs.
VI. Collaborators and Other Affiliations:
a) Collaborators (M.S. and Ph.D. level only):
Graham, Terrence L., Department of Plant Pathology, Ohio State University
Yu, Oliver, Donald Danforth Plant Science Center, St. Louis
Submanian, Santhil, Donald Danforth Plant Science Center, St. Louis
St. Martin, Steve, Department of Horticulture and Crop Science, Ohio State University
b) Graduate and Post-doctoral Advisors (non-retired; with current affiliations):
Olson, Maynard (Department of Genetics, University of Washington, Seattle)
Boime, Irving (Department of Pharmacology, Washington University, St Louis)
c) Activity for advising graduate and undergraduate students (within last 5 years):
Thesis advising:
Larue, Clayton: M.S. informal co-advising (currently: post-doctoral researcher, Univ. of Illinois)
Cheng, Jiye: Ph. D. informal co-advising (current: Plant-Pathology and PMBB program)
Outreach undergraduate training:
Garrett, Katy: advising for undergraduate research competition (OSU Denman).
Riggs, Kara: co-advising and providing some material.
Terrence L. Graham
Professor, Department of Plant Pathology
Plant Molecular Biology and Biotechnology Program
The Ohio State University
(i). Professional Preparation:
B.S.
Biochemistry, Suma cum laude, Pennsylvania State University
M.S.
Biochemistry, Purdue University
Ph.D.
Biochemistry, Purdue University
Post-Doctoral
Plant Pathology, University of Wisconsin (with Luis Sequeira, Academy
of Science)
(ii). Appointments:
2002Professor, Department of Plant Pathology, The Ohio State University.
1996-2000
Associate Chair, Department of Plant Pathology, The Ohio State University
1992-2002
Associate Professor, Department of Plant Pathology, The Ohio State University.
1986-1992
Assistant Professor, Department of Plant Pathology, The Ohio State University.
1984-1986
Research Manager and Senior Science Fellow, Cellular and Molecular Biology,
Monsanto Company
1982-1984
Science Fellow, Biological Control, Monsanto Company
1979-1982
Senior Research Group Leader, Stress Biochemistry Group, Monsanto Company
1977-1979
Project Leader, Host-Pathogen Project, Monsanto Company
(iii). Selected Honors & Other Recognition:
Honorary Fraternities: Phi Eta Sigma, Phi Kappa Phi, Phi Lambda Upsilon (Chemistry Honorary)
Charles Gerth Scholarships (Biochemical Scholastic), Pennsylvania State University.
Evan Pugh Silver Medal (Upper 0.5% of Junior Class), Pennsylvania State University.
Evan Pugh Gold Medal (Upper 0.5% of Senior Class), Pennsylvania State University.
Summa Cum Laude Graduate of Pennsylvania State University. Class rank, 2nd, School of Science
John White Graduate Fellowship, Pennsylvania State University.
University Graduate Fellowship, Purdue University.
Public Health Service National Research Service Awards for Post-Doctoral Training in Pathology
Monsanto Achievement and Incentive Awards (3 years).
Monsanto Science Fellow, Monsanto Senior Science Fellow
College of Food, Agricultural and Environmental Sciences, Award for Excellence in Teaching
The Ohio State University, Dept of Plant Pathology Research Award.
(iv) Selected Recent Professional Activities
Over 20 invited talks at conferences, seminars and symposia since 2003, including a plenary talk at the
Annual Meeting of the American Phytopathological Society in 2006.
Organizer of over 5 Research Symposia since 2000, including the Session “Plant Cell Death” at the
International Congress of Stress, Budapest, Hungary, 2007.
(vi) Selected Recent Publications
Pan L, Sinden MR, Kennedy AH, Chai H, Watson LE, Graham TL, Kinghorn AD. 2008. Bioactive
constituents of Helianthus tuberosus (Jerusalem artichoke). Phytochemistry Letters, in press. DOI
information: 10.1016/j.phytol.2008.10.003
Graham TL, Graham MY and Yu O. 2008. Genomics of Soybean Secondary Product Metabolism. In:
Genetics and Genomics of Soybean, Stacey G, ed. Springer Verlag, NY, pp. 241-242.
Graham TL, Subramanian S, Graham MY, Yu O. 2007. RNAi silencing of genes for elicitation or
biosynthesis of 5-deoxyisoflavonoids suppresses race specific resistance and hypersensitive cell
death in Phytophthora sojae infected tissues. Plant Physiol. 144: 728-740.
Subramanian S, Graham MY, Yu O, Graham TL. 2005. RNA interference of soybean isoflavone
synthase genes leads to silencing in tissues distal to the transformation site and to enhanced
susceptibility to Phytophthora sojae. Plant Physiol. 137:1-9.
M. Y. Graham, J. Weidner, K. Wheeler, M. L. Pelow and T. L. Graham. 2003. Pathogenesis-Related
Protein Gene Activation in Soybean by Wounding and the Phytophthora sojae Cell Wall Glucan
Elicitor, Physiological and Molecular Plant Pathology 63:141-149.
T.L. Graham, P.E. Bonello. 2003. Pathogen resistance in plants, McGraw-Hill Yearbook of Science and
Technology.
Landini, S., Graham, M. Y. and Graham, T. L. 2002. Lactofen induces isoflavone accumulation and
glyceollin elicitation competency in soybean. Phytochemistry. Special Issue on Metabolomics.
62:865-874.
Park, D.S., Graham, M. Y., Landini, S. and Graham,T.L. 2002. Induced distal defense potentiation
against Phytophthora sojae in soybean. Physiological and Molecular Plant Pathology, 60:293-310.
Park, D.S., Graham, M. Y. and Graham, T. L. 2001. Identification of soybean elicitation competency
factor, CF-1, as the soybean Kunitz trypsin inhibitor. Physiological and Molecular Plant
Pathology 59:265-273.
Abbasi, P. A., M. Y. Graham and T. L. Graham. 2001. Effects of soybean genotype on the glyceollin
elicitation competency of cotyledon tissues to Phytophthora sojae glucan elicitors, Physiological
and Molecular Plant Pathology 59:95-105.
Abbasi, P. A. and T. L. Graham. 2001. Age-related regulation of induced isoflavone responses in
soybean lines differing in inherent elicitation competency. Physiological and Molecular Plant
Pathology 59:143-152.
Hsieh, M. C. and T. L. Graham. 2001. Partial purification and characterization of a soybean _glucosidase with high specific activity for isoflavone conjugates. Phytochemistry 58:995-1005.
In Preparation: Graham TL, Yu O, Subramanian S, Huge R, Sinden MR, Graham MY. 2008. Effects
of RNAi silencing of PR-1a, PR-2 and a unique metallothionein-like gene on partial and racespecific resistance to Phytophthora sojae.
(vii). Synergistic Activities:
Ohio State University representative (1996-present; Chair, 2000-2002) to the Ohio Plant
Biotechnology Consortium. Panel member for this entire period for their competitive grants program.
Associate Editor, Phytopathology (1997-1999). Editorial Board, Plant Physiology (1988-1992) and
Journal of Medicinal Food (1998-2002).
(viii) High School, Undergraduate, Graduate and Postgraduate Scholars Sponsored:
Thesis advisor for 7 Ph.D. and 1 M.S. student. Student Advisory Committees of over 57 Ph.D. and 12
M.S. students. Hosted over 10 undergraduate researchers and 8 high school students. Many of my
students have won awards, a notable one being an undergraduate (Kara Riggs) who was the first
freshmen ever to win a Denman Undergraduate Research Award.
CURRICULUM VITAE
Erich Grotewold
Present Address:
Plant Biotechnology Center
206 Rightmire Hall, 1060 Carmack Road
The Ohio State University, Columbus, OH 43210
Phone: (614) 292-2483; FAX: (614) 292-5379; E-mail: [email protected]
Professional Preparation
1988
Ph.D., Chemistry, Instituto de Ingenieria Genetica y Biologia Molecular, and
University of Buenos Aires, Buenos Aires, Argentina.
1985
B.Sc., Chemistry, Faculty of Science, University of Buenos Aires, Argentina
Academic Appointments
8/08 - present
Associate Director, Arabidopsis Biological Resource Center (ABRC), The Ohio
State University, Columbus, OH.
9/06 – present
Professor, Department of Plant Cellular and Molecular Biology and Department
of Horticulture and Crop Sciences, The Ohio State University, Columbus, OH.
9/07 - 6/08
Visiting Professor, Mathematical Bioscience Institute, The Ohio State University,
Columbus, OH.
2001 – 2006
Associate Professor, Department of Plant Cellular & Molecular Biology
Associate Professor, Department of Horticulture and Crop Sciences, The Ohio
State University, Columbus, Ohio
1997 - 2001
Assistant Professor, Department of Plant Biology
Assistant Professor, Department of Horticulture and Crop Sciences, The Ohio
State University, Columbus, Ohio
1995 - 1997
Assistant Investigator
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
1993 - 1995
Staff Associate
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
1989 - 1993
Post-Doctoral Research Fellow
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Consulting Positions
1/04 – 12/05
Consultant CERES, Inc. Thousand Oaks, CA.
Honors and Awards
2006
1995
1989 - 1990
1986 - 1989
1985 - 1986
Dean’s Award for Excellence in Undergraduate Research Mentoring
Demerec-Kaufmann-Hollaender Fellow in Developmental Genetics
Long Island Biological Association Fellow
Fellow of the National Research Council of Argentina
University of Buenos Aires Fellow
Recent Peer Reviewed Publications:
1. Yilmaz, A., Nishiyama, M.Y., Garcia-Fuentes, B., Souza, G.M., Janies, D., Gray, J. and Grotewold,
E. (2009) GRASSIUS: A platform for comparative regulatory genomics across the grasses. Plant
Physiol. In Press.
2. Xie, Z., and Grotewold, E. (2008) Serial ChIP as a tool to investigate the co-localization or
exclusion of proteins on plant genes. Plant Methods 4: 25.
3. Zhao, M., Morohashi, K., Hatlestad, G., Grotewold, E. and Lloyd, A. (2008) The TTG1-bHLH-MYB
complex controls trichome cell fate and patterning through direct targeting of regulatory loci.
Development. 135: 1991-1999.
4. Grotewold, E. (2008) Transcription factors for predictive plant metabolic engineering: Are we there
yet? Curr. Opin. Biotech. 19: 138-144.
5. Hernandez, J.M., Feller, A., Morohashi, K., Frame, K. and Grotewold, E. (2007) The bHLH domain
of maize R links transcriptional regulation and histone modifications by recruitment of an EMSYrelated factor. Proc. Natl. Acad. Sci. USA. 104: 17222-17227.
6. Morohashi, K., Zhao, M., Yang, M., Read, B., Lloyd, A., Lamb, R., and Grotewold, E. (2007)
Participation of the Arabidopsis bHLH factor GL3 in trichome initiation regulatory events. Plant
Physiol. 145: 736-746.
7. Poustka, F., Irani, N.G., Feller, A., Lu, Y., Pourcel, L., Frame, K., Grotewold, E. (2007) A trafficking
pathway for anthocyanins overlaps with the endoplasmic reticulum-to-vacuole protein sorting route
in Arabidopsis and contributes to the formation of vacuolar inclusions. Plant Physiol. 145: 13231335.
8. Serpa, V., Vernal, J., Lamattina, L., Grotewold, E., Cassia, R., Terenzi, H. (2007) Inhibition of
AtMYB2 DNA-binding by nitric oxide involves cysteine S-nitrosylation. Biochem. Biophys Res.
Comm. 361: 1048-1053.
9. Feller, A., Hernandez, J.M., and Grotewold, E. (2006) An ACT domain participates in the
dimerization of several plant bHLH transcription factors. J. Biol. Chem. 281: 28964 – 28974.
10. Vargo, M.A., Voss, O.H., Poustka, F., Cardounel, A.J., Grotewold, E., and Doseff, A.I. (2006)
Apigenin-induced-apoptosis is mediated by the activation of PKCδ and caspases in leukemia cells.
Biochem. Pharmacol. 72: 681-692.
11. Palaniswamy, K., James, S., Sun, H., Lamb, R., Davuluri, R.V. and Grotewold, E. (2006) AGRIS
and AtRegNet: A platform to link cis-regulatory elements and transcription factors into regulatory
networks. Plant Phyisiol. 140: 818-829.
12. Grotewold, E. (2006) The genetics and biochemistry of flower pigments. Ann. Rev. Plant Biol. 57:
761-780.
13. Lu, Y., Irani, N.G., and Grotewold, E. (2005) Covalent attachment of the plant natural product
naringenin to small glass and ceramic beads. BMC Chemical Biology 5: 3.
14. Molina, C., and Grotewold, E. (2005) Genome-wide analysis of Arabidopsis core promoters. BMC
Genomics 6: 25.
15. Barg, R., Sobolev, I., Eilon, T., Gur, A., Chmelnitsky, I., Shabtai, S., Grotewold, E. and Salts, Y.
(2005) The tomato early fruit specific gene Lefsm1 defines a novel class of plant-specific
SANT/MYB domain proteins. Planta 221: 197-211.
Books Edited
Grotewold, E. (Editor) The Science of Flavonoids. (2006) Springer, New York, NY.
Grotewold, E. (Editor) Plant Functional Genomics: Methods & Protocols. (2003) Humana Press,
Totowa, NJ.
Patents and Inventions
Transgenic plants with altered levels of phenolic compounds. Pat. No. 7,154,023.
Transgenic turfgrasses which signal exposure to chemicals and stress conditions. Pat. No. 6,709,867
Inhibition of monocyte survival, differentiation, or proliferation. Application No. PCT/US06/020905
Synergistic Activities:
Director, OSU Summer Functional Genomics Training Workshop (2005)
Founder, Vice-Chair (2005) and Chair (2007), Gordon Research Conference in Plant Metabolic
Engineering
Founder and Vice-Chair (2007) Gordon-Kenan Graduate Research Seminars in Plant Metabolic
Engineering
Member in Interdisciplinary Training Programs: Molecular, Cellular and Developmental Biology
(MCDB), Biophysics and Ohio State Biochemistry Program (OSBP)
Member, Advisory Committee for the NSF-funded OSU Mathematical Biosciences Institute
HAMEL, Patrice
BIOGRAPHICAL SKETCH
NAME
POSITION TITLE
Patrice Hamel
Assistant Professor
EDUCATION/TRAINING
DEGREE
(if applicable)
INSTITUTION AND LOCATION
University of Paris XI, Orsay, France
University of Paris XI, Orsay, France
University of California, Los Angeles
YEAR(s)
M.S. (eq.)
Ph.D.
post-doctoral
FIELD OF STUDY
1993 Cellular and Molecular Genetics
1999 Cellular and Molecular Genetics
1999-2005 Organellar Biochemistry
A. PROFESSIONAL POSITIONS AND HONORS
Previous positions
From November 1999-June 2005: Post-graduate Researcher Department of Chemistry &
Biochemistry, University of California, Los Angeles
July 2005-October 2005: Assistant Project Scientist, Department of Chemistry & Biochemistry,
University of California, Los Angeles
Present positions
From November 2005: Assistant Professor, Department of Plant and Cellular Molecular Biology and
Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus.
Other professional activities
-Reviewer for EMBO Journal, Biochimica and Biophysica Acta, Molecular and General Genetics,
Molecular Microbiology, FEBS letters, Proceedings of the National Academy of Sciences USA, Plant
Journal, Journal of Biological Chemistry, Eukaryotic Cell, Biochemistry and Journal of Bacteriology.
-Ad hoc reviewer for USDA, NSF
Memberships:
Associate Investigator, the Dorothy M. Davis Heart and Lung Research Institute, the Ohio State
University
Member of the Institute of Mitochondrial Biology, The Ohio State University
Genetics Society of America
Honors and Awards
American Heart Association Post-doctoral Fellowship, 2001-2003
Paul Boyer Award for Excellence in Post-doctoral Research, 2003
American Heart Association Post-doctoral Fellowship, 2003 (declined, duplicate funding)
Muscular Dystrophy Association Research Development Award 2003-2006
B. RESEARCH PUBLICATIONS (in chronological order)
(1) Bonnefoy, N., Chalvet, F., Hamel P., Slonimski, P.P. & Dujardin G., (1994) OXA1, a Saccharomyces
cerevisiae nuclear gene whose sequence is conserved from prokaryotes to eukaryotes controls cytochrome
oxidase biogenesis. J. Mol. Biol., 239, 201-212.
(2) Hamel, P., Sakamoto, W., Wintz, H. & Dujardin, G., (1997) Functional complementation of an oxa1 yeast
mutation identifies an Arabidopsis thaliana cDNA involved in the assembly of respiratory complexes. Plant J.,
12, 1319-1327.
1
HAMEL, Patrice
(3) Hamel, P., Lemaire, C., Bonnefoy, N., Brivet-Chevillotte, P. & Dujardin G., (1998) Mutations in the
membrane anchor of yeast cytochrome c1 compensate for the absence of Oxa1p and generate carbonateextractable forms of cytochrome c1. Genetics, 150, 601-611.
(4) Cardazzo, B., Hamel, P., Wintz, H., Sakamoto, W. & Dujardin, G., (1998). Isolation of an Arabidopsis
thaliana cDNA by complementation of a yeast abc1 deletion mutant deficient in complex III respiratory activity.
Gene 221, 117-125.
(5) Arai, M., Hamel, P., Kanaya, E., Inaka, K., Miki, K., Kikuchi, M. & Kuwajima, K. (2000) Effect of an
alternative disulfide bond on the structure, stability and folding of human lysozyme. Biochemistry 39, 34723479.
(6) Hamel, P., Olive, J., Pierre, Y., Wollman, F.-A. & de Vitry, C. (2000) A new subunit of cytochrome b6 f
complex undergoes reversible phosphorylation upon state transition. J. Biol. Chem. 275, 17072-17079.
(7) Lemaire, C., Hamel, P., Velours, J. and Dujardin, G. (2000) Absence of the mitochondrial AAA protease
Yme1p restores ATPase F0 subunit accumulation in an oxa1 deletion mutant of Saccharomyces cerevisiae. J.
Biol. Chem. 275, 23471-23475.
(8) Nakamoto, S., Hamel, P. & Merchant, S., (2000) Assembly of chloroplast cytochromes b and c. Biochimie
82, 603-614.
(9) Saint-Georges, Y., Hamel, P., Lemaire, C., and Dujardin, G. (2001) Role of positively-charged
transmembrane segments in the insertion and assembly of mitochondrial inner-membrane proteins. PNAS 98,
13814-13819.
(10) Hamel, P., Dreyfuss, B., Xie, Z., Gabilly, S. and Merchant S. (2003) Essential histidine and tryptophan
residues in CcsA, a system II polytopic cytochrome c biogenesis protein. J. Biol. Chem. 278, 2593-2603.
(11) Dreyfuss, B., Hamel, P., Nakamoto, S. and Merchant S. (2003) Functional analysis of a divergent system
II protein, Ccs1, involved in c-type cytochrome biogenesis. J. Biol. Chem. 278, 2604-2613.
(12) Bernard, D., Gabilly, S., Dujardin, G., Merchant, S. and Hamel, P. (2003) Overlapping specificities of the
cytochrome c and c1 heme lyases. J. Biol. Chem. 50, 49732-49742.
(13) Hamel, P., Saint-Georges, Y., Altamura N., de Pinto, B. and Dujardin, G. (2004) Redundancy in the
function of mitochondrial phosphate transport in Saccharomyces cerevisiae and Arabidopsis thaliana. Mol.
Microbiol. 51, 307-317.
(14) Page, M*., Hamel, P*., Gabilly, S., Zegzouti, H, Perea, J., Alonso, J., Ecker, J., Theg, S., Christensen, S.,
and Merchant, S. (2004) A homolog of the prokaryotic thiol-disulfide transporter CcdA is required for the
assembly of the cytochrome b6f complex in Arabidopsis chloroplasts. J. Biol. Chem. 279, 32474-32482. (*
equal contribution).
(15) Bernard, D.B., Quevillon-Cheruel, S., Merchant S., Guiard, B. and P. Hamel (2005) Cyc2p, a membrane
bound flavoprotein involved in the maturation of mitochondrial c-type cytochromes. J. Biol. Chem. 280, 3985239859.
(16) Hanikenne M., Merchant, S. and Hamel, P. (in press) Transition metal nutrition: a balance between
deficiency and toxicity. Chlamydomonas in the Plant Science, Vol.2 of the Chlamydomonas Sourcebook (Ed.
D. Stern, Publisher: Elsevier Inc.).
(17) Remacle C., Barbieri, R., Cardol P., and Hamel, P. Eukaryotic complex I: functional diversity and
experimental systems to unravel the assembly process Molecular Genetics and Genomics 280, 93-110.
3
HAMEL, Patrice
(18) Hamel P., Corvest V., Giege P and Bonnard G.. Biochemical requirements for the maturation of
mitochondrial c-type cytochromes Biochimica Biophysica Acta-Molecular Cell Research (in press,
DOI:10.1016/j.bbamcr.2008.06.017).
(19) Claire, R., Cline, S., Boutafalla, L., Gabilly, Larosa, V., Barbieri, R. M. and Hamel, P The ARG9 gene
encodes the plastid resident N-acetyl ornitine aminotransferase in the green alga Chlamydomonas reinhardtii
Eukaryotic Cell (under revision).
C. RESEARCH SUPPORT
On-going-research support:
Unraveling the mitochondrial redox pathway in cytochrome c maturation
Principal investigator: Patrice Hamel
Agency: Muscular Dystrophy Association
Type: Neuromuscular disease research grant MDA4247 (Years 07-09) Period: 1 Jan, 2007 - 31 Dec, 2009
This project focuses on the genetic and biochemical dissection of the intermembrane space thioredox pathway
operating in the maturation of c-type cytochromes in yeast mitochondria.
Molecular genetic dissection of mitochondrial complex I assembly
Principal investigator: Patrice Hamel
Agency: United Mitochondrial Disease Foundation
Type: Research grant Period: 17 Jul, 2007 - 16 June, 2009
This project aims to discover novel assembly factors for mitochondrial complex I using insertional mutagenesis
of the nucleus in the green alga Chlamydomonas reinhardtii.
Exploration of cellular functions of organellar coiled-coil proteins in plant model systems. Do plastid
and mitochondria have a cytoskeleton-like structure?
Principal investigator: Iris Meier
Co-principal investigator: Patrice Hamel
Agency: Ohio Plant Biotechnology Consortium
Type: Research grant Period: 1 Jul, 2008 – 30 June, 2010
This project aims to investigate in Arabidopis and Chlamydomonas the function of a class of novel proteins
called coiled-coil proteins with mitochondrial or plastid localization.
Conservation of molecular mechanisms controlling the biogenesis of respiratory and photosynthetic
complexes
Principal investigator: Geneviève Dujardin (CNRS)
Co-principal investigator: Patrice Hamel
Agency: Centre National de la Recherche Scientifique (CNRS, France)
Type: Travel grant Period: 1 Jan, 2008 – 31 Dec, 2010
This grant aims to support international collaboration at major american institutions for projects that are already
on-going in the american host laboratory. The funds can only be used to support the visit of members from a
french laboratory to the PI’s group. The co-PI has no financial responsibility over this grant.
Assembly of mitochondrial c-type cytochromes: Emergence of novel redox factors
Principal investigator: Vincent Corvest
Co-principal investigator: Patrice Hamel
Agency: American Heart Association
Type: Post-doctoral fellowship Period: 1 Jul, 2008 – 30 June, 2010
3
HAMEL, Patrice
The goal of this project is to decipher the biochemical activity of Cyc2p, a novel flavoprotein involved in the
assembly of c-type cytochromes in yeast mitochondria.
Regulation of H2 and CO2 Metabolism: Factors Involved in Partitioning of Photosynthetic Reductant in
Green Algae
Principal investigator: Maria Ghirardi (NREL)
Co-principal investigator: Patrice Hamel
Agency: Department of Energy
Type: Research grant Period: 1 Oct, 2008 – 30 Sept, 2011
The goal of this project is to generate an insertional mutant library in Chlamydomonas and screen for strains
that are attenuated for hydrogen production. The long term goal is to uncover the genes controlling this
process in order to engineer algal strains that overproduce hydrogen.
Completed research support:
Functional dissection of a cytochrome c assembly machinery in mitochondria
Principal investigator: Patrice Hamel
Agency: Muscular Dystrophy Association
Type: Neuromuscular disease research development grant MDA3618 (Years 01-03) Period: 1 Jul, 2003 - 30
Sept, 2006 (no cost extension)
This project focuses on the functional identification of novel components involved in a c-type cytochrome
assembly complex in yeast mitochondria
Unravelling the mechanisms of cytochrome c biogenesis
Principal investigator: Patrice Hamel
Agency: American Heart Association Western Affiliates
Type: Post-doctoral Fellowship 0120100Y (Years 01-02) Period: 1 Jul,2001 - 30 Jun, 2003
The goals of this project were to define the biochemistry of well-characterized c-type cytochrome assembly
components in plastids of green alga and mitochondria of yeast and discover novel biogenesis factors.
3
CURRICULUM VITAE
Jyan-Chyun Jang
Professional Preparation
Natl. Taiwan University
Natl. Taiwan University
Clemson University
Inst. of Biological Chemistry
Washington State Univ.
Department of Genetics
Harvard Medical School
Dept. of Mol. Biol.,
Mass. General Hospital
Forest Botany
Plant Pathology
Forest Pathology
1980 B.Sc.
1984 M.Sc.
1989 Ph.D.
Molecular biology
1990-1991 Postdoctoral Researcher
Genetics
1991-1997 Postdoctoral fellow
Molecular Biology
1991-1997 Postdoctoral fellow
Appointments
2003-present Associate Professor
Dept. of Horticulture and Crop Science
Dept of Plant Cellular and Molecular Biology, The Ohio State University
1997-2002
Assistant Professor
Dept. of Horticulture and Crop science
Dept of Plant Biology, The Ohio State University
1997-present Member
Plant Molecular Biology and Biotechnology Program
Program in Molecular, Cellular and Developmental Biology, The Ohio State University
Recent Publications
1. He, J. X., Fujioka, S., Li, T.-C., Kang, S. G., Seto, H., Takatsuto, S., Yoshida, S., and
Jang, J.-C. (2003) Sterols regulate development and gene expression in Arabidopsis
thaliana. Plant Physiol. 131:1258-1269.
2. Price, J., Li, T. C., Kang, S. G., Na, J. K., and Jang, J.-C. (2003). Mechanisms of
glucose signaling during seed germination of Arabidopsis thaliana. Plant Physiol.
132 (3): 1424-1438.
3. Price, J., Laxmi, A., St. Martin, S. K., and Jang, J.-C. (2004) Global transcription
profiling reveals multiple sugar signal transduction mechanisms in Arabidopsis.
Plant Cell 16:2128-2150.
4. Shao, M., Zheng, H., Hu, Y., Liu D., Jang, J-C., Ma, H., and Huang, H. (2004) The
GAOLAOZHUANGREN1 gene encodes a putative glycosyltransferase that is required
for normal development and affects sink-source transition in Arabidopsis. Plant Cell
Physiol. 45:1453-1460.
5. Zheng Z-L, Yang Z, Jang J-C and Metzger JD (2006) Phytochromes A1 and B1 have
distinct functions in the photoperiodic control of flowering in the obligate long-day
plant Nicotiana sylvestris. Plant Cell & Environment 29: 1673-1685
Current Advising:
Graduate (Ph.D.): Marcelo Pomeranz, Srimathi Bogamuwa, Jie Qu
Graduate (MS): Li Zhang
Postdoc: Shin Gene Kang
Past Thesis Advisor and Postdoc Sponsor
PhD: Wenyan Xiao (Faculty, St. Louis University), 1997-2001
Shin Gene Kang (Postdoc, OSU), 1999-2004
Jong Kuk Na (Postdoc, U of Illinois), 2001-2005
Tsai-Chi Li (Graduate student, OSU), 2001-2006
Pei-Chi Lin (deceased), 2004-2007
MS: Marie Purtell (Kendal International, Cincinnati, OH), 2001-2003.
Postdoc: Jun Xian He (Faculty, Chinese University of Hong Kong), 2000-2001
Ashverya Laxmi (Faculty, University of Delhi, India), 2003-2004
Minghai Shao (Postdoc, Children’s Hospital, Columbus, OH), 2004
Sholpan Devaletova (Postdoc, Children’s Hospital, Columbus, OH), 2004
Janaiah Kota (Postdoc, Children’s Hospital, Columbus, OH), 2005
Current Course Taught
694 Microarray Analysis for Gene Expression Profiling
830 Signal Transduction of Plant Growth Substances
830 Research Methods: Transient Gene Expression
Synergistic Activities
Grant Panel: USDA CSREES (2007)
Ad Hoc Review: NSF, USDA, BARD, Irish National Science Foundation, CUNY
Journal Article Review: Arch Biochem Biophy, BMC Systems Biology, BMC Plant Biology,
Functional Plant Biology, Genome Research, J of American Society for Horticultural
Science, J. Experimental Botany, J. of Plant Growth Regulation, Molecular Biology Reports,
Molecular Plant, New Phytologists, Physiol Plant, Plant Cell, Plant Cell and Environment,
Plant Cell Physiology, Plant Cell Rept, Plant Journal, Plant Mol Biol, Plant Physiology,
Plant Physiol Biochem, Planta, PLoSONE, PNAS, Seed Science & Technology, Trends Plant
Sci.
Reecent Extramural Fundings
USDA CSREES (2005-3531915318)
Jan. 1, 2005 to Dec. 31, 2007
$387,500
JC Jang (PI), Wolfgang D. Bauer (Co-PI)
NSF Biological Infrastructure (DBI-0500601)
May 1, 2005 to April 30, 2008
$67,111
David Somers (PI), Erich Grotewold (Co-PI), JC Jang (Co-PI)
NSF Integrative Organismal Biology (IOB-043751)
Feb. 1, 2006 to Jan. 31, 2010
$480,000 JC Jang (PI)
BIOGRAPHICAL SKETCH
MICHELLE L. JONES
Department of Horticulture and Crop Science, The Ohio State University/ OARDC
Wooster, OH, 44691; Tel: 330-263-3885; Fax: 330-263-3887; email: [email protected]
Professional Preparation:
Iowa State University, Ames, IA
Purdue University, West Lafayette, IN
Professional Experience:
2008- present
2006- present
2001- 2006
1998- 2001
B.S. 1993
Ph.D. 1997
Agricultural Biochemistry
Horticulture
D.C. Kiplinger Endowed Chair in Floriculture
Dept. of Horticulture and Crop Science
The Ohio State University, Wooster, OH
Associate Professor, Dept. of Horticulture and Crop Science, The
Ohio State University, Wooster, OH
Area of Specialization: Floriculture Molecular Biology
Assistant Professor, Dept. of Horticulture and Crop Science, The
Ohio State University, Wooster, OH
Assistant Professor, Dept. of Horticulture and Landscape
Architecture, Colorado State University, Fort Collins, CO.
Publications (5 most relevant):
Jones M.L. (2008) Ethylene signaling is required for pollination-accelerated corolla senescence in
petunias. Plant Science 175: 190-196.
Chapin L., and M.L. Jones (2007) Nutrient remobilization during pollination-induced corolla
senescence in petunia. Acta Horticulturae 755: 181-190.
Jones M.L., G.S. Chaffin, J.R. Eason and D. Clark (2005) Ethylene Sensitivity Regulates
Proteolytic Activity and Cysteine Protease Gene Expression in Petunia Corollas. Journal of
Experimental Botany. 56:2733-2744.
Chiang Y-C., C. Stushnoff, A.E. McSay, M.L. Jones and H. Bohnert (2005) Overexpression of
Mannitol-1-Phosphate Dehydrogenase Increases Mannitol Accumulation and Adds Protection
Against Chilling Injury in Petunia. Journal of the American Society for Horticultural Science.
130:605-610.
Langston B.L., S. Bai and M.L. Jones (2005) Increases in DNA Fragmentation and Induction of a
Senescence-Specific Nuclease are delayed during the Senescence of Ethylene-insensitive (etr1-1)
Transgenic Petunias. Journal of Experimental Botany. 56:15-23.
Publications (5 significant):
Jones, M.L., Shuangyi B., Willard B., Stead A., and M. Kinter (2007) Proteomic Analysis of
Pollination-induced Senescence in Petunia Flowers. Proceedings of the 7th International Ethylene
1
Symposium. A. Ramina, C. Chang, J. Giovannoni, H. Klee, P. Perata, and E. Woltering eds.
Springer, The Netherlands, Pgs. 279-284.
Pennycooke J.C., R. Vepachedu, C. Stushnoff and M.L. Jones (2004) Expression of an αGalactosidase Gene in Petunia is Up-regulated during Low Temperature Deacclimation. Journal of
the American Society for Horticultural Science. 129:491-496.
Clark D.G., C. Dervinis, J.E. Barrett, H. Klee and M.L. Jones (2004) Drought-induced Leaf
Senescence and Horticultural Performance of Transgenic PSAG12-IPT Petunias. Journal of the
American Society for Horticultural Science. 129: 93-99.
Pennycooke J.C., M.L. Jones and C. Stushnoff (2003) Down-Regulating α-Galactosidase Enhances
Freezing Tolerance in Transgenic Petunia. Plant Physiology. 133: 901-909.
Chang H., M.L. Jones, G.M. Banowetz and D.G. Clark (2003) Overproduction of Cytokinins in
Petunia Flowers Transformed with PSAG12-IPT Delays Corolla Senescence and Decreases Sensitivity
to Ethylene. Plant Physiology. 132: 2174-2183.
Synergistic activities:
Consulting Editor, Molecular Biology and Biotechnology, The Journal of the American Society for
Horticultural Science, 2004 to present.
Member Editorial Board, Postharvest Biology and Technology, 1999 to 2004.
Collaborators and Other Affiliations:
Collaborators and Co-authors:
Bai, Shuangyi (Washington State); Banowetz, Gary (ARS, Corvallis); Barrett, Jim (U Florida):
Bohnert, Hans (U of Illinois); Canas Luis (Ohio State); Chaffin, Gunching (Unknown); Clark, David
(U Florida); Dervinis, Chris (U Florida); Eason, Joycelyn (Food & Crop Research, NZ); Finer, John
(Ohio State); Francis, David (Ohio State); Franz, Jonathan (ARS, Toledo); Johnson, Felicity
(Unnknown); Kinter, Michael (Cleveland Clinic, OH); Klee, Harry (U Florida); Langston, Brennick
(TX A&M); Lewandowski, Dennis (Ohio State); Ling, Peter (Ohio State); Lopez, Roberto (Purdue),
Meulia, Tea (Ohio State); Pasian, Claudio (Ohio State); Pennycooke, Joyce (Seminis, CA); Stead,
Anthony (Royal Holloway, UK); Stushnoff, Cecil (Colorado State); Willard, Belinda (Cleveland
Clinic, OH), Woodson, William (Purdue)
Advisors:
William R. Woodson, Purdue University
Current Advisees:
Ph.D. Nicole Waterland, Youyoun Moon
Past Advisees:
Postdoctoral researchers: Gunching Chaffin, Felicity Johnson
Ph.D. Shuangyi Bai (Washington State Univ), Hsiang Chang (Chungchou Institute of Technology,
Taiwan), Joyce Pennycooke (Seminis)
2
M.S. Brennick Langston (Texas A&M Univ)
3
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Lamb, Rebecca Sarah
Assistant Professor Plant Cellular and Molecular
Biology
eRA COMMONS USER NAME
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
DEGREE
(if applicable)
Wesleyan University Middletown, CT
Duke University Durham, NC
BA
Ph.D.
Yale University New Haven, CT
NA
YEAR(s)
1992
1998
1998-2003
FIELD OF STUDY
Biology/German Studies
Zoology and Cell and
Molecular Biology
Floral development in
Arabidopsis thaliana
A. Positions and Honors.
Positions and Employment
1998-2003 Postdoctoral Researcher, Yale University, New Haven, CT
2003Assistant Professor, Plant Cellular and Molecular Biology, Plant Molecular Biology and
Biotechnology Program, and The Molecular, Cellular, and Developmental Biology
Program, The Ohio State University
Professional Memberships
1999Member, American Society of Plant Biologists
2003Member, American Society for the Advancement of Science
2003Member, Genetics Society
2004Member, Society for Developmental Biology
2004Member, FASEB
2007Member, Association for Women in Science
Professional Activities
2003Reviewer, Plant Cell, Plant Science, PNAS, Plant Physiology, Development, Science,
Nature
2005Panelist, National Science Foundation, Epigenetics, Transcription and Chromatin Panel
within the Genes and Genome Systems, BIO/MCB
Fellowships and Honors
1988-1992 Hedden Scholarship, Wesleyan University, Middletown, CT
1992
Blankennagel Prize in German, Wesleyan University, Middletown, CT
1992-1995 NIH Training Grant in Cell and Molecular Biology, Duke University, Durham, NC
1998-1999 Brown Postdoctoral Fellowship, Yale University, New Haven, CT
B. Selected peer-reviewed publications (in chronological order).
1. Morohashi, K, Zhao, M, Yang, M, Read, B, Lloyd, A, Lamb R, and Grotewold, E (2007).
Participation of the Arabidopsis bHLH factor GL3 in trichome initiation regulatory events. Plant
Physiology 145(3): 736-746.
2. Palaniswamy, S., James, S., Sun H, Lamb, R.S., Davuluri, R.V., Grotewold, E (2006). AGRIS and
AtRegNet: A platform to link cis-regulatory elements and transcription factors into regulatory
networks. Plant Physiology 140(3): 818-829.
3. Lamb, R.S. and Irish, V.F. (2003) Functional divergence within the APETALA3/PISTILLATA floral
homeotic gene lineages. Proc. Nat. Acad. Sci. 100: 6558-6563.
4. Lamb, R.S., Hill, T.A., Tan, Q.K., and Irish, V.F. (2002) Regulation of APETALA3 floral homeotic
gene expression by meristem identity genes. Development 129: 2079-86.
5. Ward, R.E., Schweizer, L, Lamb, R.S., and Fehon, R.G. (2001) The protein 4.1, radixin, moesin
(FERM) domain of Drosophila Coracle, a cytoplasmic component of the septate junction, provides
functions essential for embryonic development and imagninal cell proliferation. Genetics 159: 219228.
6. Lamb, R.S., Ward, R.E., Schweizer, L. and Fehon, R.G. (1998) Drosophila coracle, a member of
the protein 4.1 superfamily, has essential structural functions in the septate junctions and
developmental functions in the embryonic and adult epithelial cell. Mol. Biol. Cell 9: 3505-3519.
7. Ward, R.E., Lamb, R.S., and Fehon, R.G. (1998) A conserved functional domain of Drosophila
Coracle is required for localization at the septate junction and has membrane organizing activity. J.
Cell Biol. 140: 1463-1473.
8. Fehon, R.G., LaJeunesse, D, Lamb, R.S., McCartney, B.M., Schweizer, L., and Ward, R.E. (1997)
Functional studies of the protein 4.1 family of junctional proteins in Drosophila. Soc. Gen. Physiol.
Ser. 52: 149-159.
C. Research Support
Ongoing Research Support
Ohio Plant Biotechnology Consortium
Research Grant
7/1/2007-7/30/2009
Two Arabidopsis WWE-PARP Proteins Involved in Abiotic Stress Response and Development.
The major goal of this project is characterize the phenotypic and molecular phenotypes of two
Arabidopsis genes that appear to be involved in epigenetic control of transcription in plants.
Role: Principal Investigator
Completed Research Support
MCB-0418891 Grotewold (PI)
NSF
9/1/2004-8/31/2007
Arabidopsis 2010: Establishing Regulatory Networks in Arabidopsis: Integrating AGRIS with the
Identification of Direct Targets for Transcription Factors
The goal of this research was to determine the genes directly controlled by a set of plant transcription
factors.
Role: Co-investigator
BIOGRAPHICAL SKETCH
Brian B. McSpadden Gardener
Department of Plant Pathology The Ohio State University/OARDC,
Wooster, OH 44691; Tel: 330-2202-3565; FAX: 330-263-3841;
email: [email protected]; web: http://www.oardc.ohio-state.edu/mcspaddengardenerlab/
PROFESSIONAL PREPARATION
University of Illinois
Honors Biology
B.Sc.
Michigan State University
Botany
Ph.D.
Washington State University
Microbial diversity/ecology Postdoc.
1992
1998
1998-2000
PROFESSIONAL EXPERIENCE
10/06 to Present Associate Professor, Department of Plant Pathology, Ohio State U.
11/00 to 09/06
Assistant Professor, Department of Plant Pathology, Ohio State U.
PUBLICATIONS most closely related to the project:
Benitez, M.-S., Baysal Tustas, F., Rotenberg, D., Kleinhenz, M.D., Miller, S.A., and McSpadden
Gardener, B.B. 2007. Multiple statistical approaches of community fingerprint data reveal bacterial
populations associated with general disease suppression arising from the application of different
organic field management strategies. Soil Biology and Biochemistry 39:2289-2301
Rotenberg, D. Joshi, R. Benitez, M.S., Gutierrez Chapin, L., Camp, A., Zumpetta, C., Osborne, A., Dick,
W.A., and McSpadden Gardener, B.B. 2007. Complex effects of farm management on rhizosphere
colonization by native populations of phlD-containing Pseudomonas spp. and the relative
contribution of those bacteria to crop stand and productivity. Phytopathology 97:756-766.
Lewis Ivey, M.L., McSpadden Gardener, B.B., Opina, N., and Miller, S.A. 2007. Diversity and
geographic distribution of Ralstonia solanacearum strains isolated from eggplant in the Luzon Region
of the Philippines. Phytopathology 97:1467-1475.
McSpadden Gardener, B. 2006. Statistical analyses of microbiological and environmental data. Chapter
22, pp. 555-585 in Modern Soil Microbiology, 2nd ed. J.D. van Elsas, J. Jannson eds. CRC Press:
Boca Raton, FL.
Joshi, R., and McSpadden Gardener, B. 2006. Identification of genes associated with pathogen inhibition
in different strains B. subtilis. Phytopathology 96:145-154.
Other significant publications:
Borneman, J., Becker, J.O., Bent, E., Lanoil, B., McSpadden Gardener, B., Olatinwo, R., Presley, L.,
Scupham, A.J., Valinsky, L., and Yin, B. 2007. Identifying microorganisms involved in specific in
situ functions: Experimental design considerations for rRNA gene-based population studies and
sequence-selective PCR assays. Pages 748-757. in Manual of Environmental Microbiology, 3rd ed.
ASM Pres: Washington, D.C.
Gutierrez Chapin, L., Want, Y., Lutton, E., and McSpadden Gardener, B. 2006. Distribution and
fungicide sensitivity of tomato fruit rot pathogens in Ohio. Plant Disease 90:397-403
McSpadden Gardener, B., Gutierrez, L., Joshi, R., Edema, R., and Lutton, E. 2005. Distribution and
biocontrol potential of phlD+ pseudomonads in corn and soybean fields. Phytopathology 95:715-724.
Blouin Bankhead, S., Landa, B., Lutton, E., and Weller D., McSpadden Gardener, B. 2004. Minimal
changes in rhizobacterial population structure following root colonization by wild type and transgenic
biocontrol strains. FEMS Microbiol. Ecol. 49:307-318.
McSpadden Gardener B., and Weller D. 2001. Changes in populations of rhizosphere bacteria associated
with take-all disease of wheat. Appl. Environ. Microbiol. 67:4414-4425
SYNERGISTIC ACTIVITIES
Multi-state research committees related to biological control: NC-125 (2002-2004), W-1147 (2006Present)
American Phytopathological Socieity Editorships: Assoc. Editor Appl. Environ, Microbiol. (2008Present), Senior Editor APS Press (2007-Present),Assoc. Editor Phytopathology (2003-2006),
American Phytopathological Society, Biological Control Committee; member, Chair 2003, web-site
developer and manager (2003-Present), Symposium organizer (2003-2006)
Poster session organizer, 18th World Congress of Soil Sciences (2005-2006)
Undergraduate Internship Coordinator Plant Molecular Biology and Biotechnology Program (2007Present)
Graduate Teaching:
PLNT PTH 655: Quantitative Methods in Applied Biology (100%)
PLNT PTH 602: Plant-Microbe Interactions (10%)
PLNT PTH 843: Ecology of Plant-Associated Microbes (50%)
PLNT PTH 830: Biological Control of Plant Pathogens (100%)
COLLABORATORS AND COAUTHORS
Beuerlein, James (Ohio State U.), Boehm, Mike (Ohio State U.), Bonello, Pieluigi (Ohio State U.),
Borneman, James (U. California), Cardina, John (Ohio State U.), Dorrance, Anne (Ohio State U.), Dick,
Warren (Ohio State U.), Dick, Richard (Ohio State U.), Driks, Adam (Loyola U.), Edema, Richard
(Makere U.), Edwards, Clive (Ohio State U.), Fravel, Deborah (USDA ARS), Fuerst, Paul (Ohio State
U.), Graham, Terry (Ohio State U.), Grewal, Parwidner (Ohio State U.), Grotewald, Erich (Ohio State
U.), Hatcher, Patrick (Ohio State U.), Hoitink, H.A.J. (Ohio State U.), Hoy, Casey (Ohio State U.),
Kinkel, Linda (U. Minnesota), Kleinhenz, Matthew (Ohio State U.), Landa, Blanca B. (U. Cordoba),
Louws, Frank (NCSU), Mavrodi, Dmitri (Washington State U.), Meyer, Susan (USDA-ARS), Michel,
Fred (Ohio State U.), Miller, Sally A. (Ohio State U.), Nakatsu, Cindy (Purdue U.), Pan, Jean (Akron U.),
Paul, Eldor (Michigan State U.), Raaijmakers, Jos (Wageningen U.), Read, Timothy (U. of Maryland),
Robertson, Philip (Michigan State U.), Rupe, John (U. Arkansas), Schroeder, Kurt (Washington State U.),
Smart, Christine (Cornell U.), Stinner, Deborah (Ohio State U.), Thomashow, Linda (Washington State
U.), Thomison, Peter (Ohio State U.), Tiedje, James (Michigan State U.), van der Knaap, Eshter (Ohio
State U.), Wang, Koon-Hui (U. Florida), Wszelaki, Annette (U. of Puerto Rico).
ADVISORS
De Bruijn, Frans J. (INRA France)
Van Elsas, Jan D. (U. Groningen, Netherlands)
Nadler, Kenneth D. (Michigan State University)
Weller, David M. (Washington State University)
ADVISEES
Current:
Ph.D. Benitez, M. Soledad
M.S. Raudales, Rosa
Past:
M.S. Gutierrez-Chapin, Laura (Ohio State U.), Joshi, Raghavendra (U. Minnesota),
Postdoctoral Researchers: Rotenberg, Dorith (Kansas State U.), Grewal, Suhkbir (Ohio State U.),
Wang, Ying (U. Tennessee)
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel in the order listed for Form Page 2.
Follow the sample format for each person. DO NOT EXCEED FOUR PAGES.
Professor of Plant Cellular and Molecular Biology NAME
Meier, Iris
POSITION TITLE
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
Technical University
Darmstadt, Germany
University of Duesseldorf,
Germany
Max Planck Institute for Plant
Breeding, Cologne, Germany.
Postdoc.
University of California at
Berkeley, CA
DEGREE
(if applicable)
YEAR(s)
FIELD OF STUDY
M.S. 1984 Microbiology Ph.D. 1987 Molecular Biology
Postdoc.
1987-1990 Plant Molecular
Biology
Postdoc.
1990-1993 Plant Molecular
Biology
A. Positions and Honors.
Positions and Employment
1993 - 1996
1997 - 1999
1999 – 2003
2003 – 2008
2008-
Junior Group Leader, Applied Plant Molecular Biology, University of Hamburg, Germany.
Senior Research Scientist, DuPont Central Research and Development, Wilmington, DE.
Assistant Professor, Dept. of Plant Biology, Ohio State University.
Associate Professor, Dept. of Plant Cellular and Molecular Biology, Ohio State University.
Professor, Dept. of Plant Cellular and Molecular Biology, Ohio State University.
Other Experience and Professional Memberships
1999Ad hoc grant reviewer for: NSF, USDA, DOE, BBSRC (UK), The Leverhulme Trust (UK),
BARD (The United States - Israel Binational Agricultural Research & Development Fund).
1999Reviewer: The Plant Cell, The Plant Journal, Plant Physiology, Journal of Cell Biology,
Transgenic Research, Planta, Plant Molecular Biology, Trends in Plant Sciences, Nucleic Acids
Research, Traffic, Biotechniques, Plant Biology.
2001Panel member: National Science Foundation, Cellular Organization Panel; United States
Department of Agriculture CSREES, Developmental Processes of Crop Plants Panel; BARD
(The United States – Israel Binational Agricultural Research and Development Fund): Model
Systems and Functional Biology Panel.
2002 - 2003 International Advisory Committee: Society for Experimental Biology (SEB) meeting
"The Nuclear Envelope: Signaling, Communication and Gene Regulation", 2003, Durham, UK.
2004 Advisory Board: NSF Plant Genome project, “Global analysis of functional units in plant
chromosomes: DNA replication, domain structure, and transcription.”
2006 Associate Editor for Plant Molecular Biology
2006 – 2008 Co-Organizer, Nuclear envelope and Golgi, Cell Biology Satellite, SEB Marseille 2008
Honors
1984 - 1986
1988 - 1990
1992 - 1993
2006
Ph.D. fellowship by the “Fond der Chemischen Industrie”, Germany
Post-doctoral fellowship by the Max-Planck-Society, Germany
Research Fellow of the German Science Foundation (DFG)
Dean’s Award for Excellence in Research and Graduate Education, College of Biological
Sciences, OSU
B. Selected peer-reviewed publications (selected from 52 research publications)
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
Meier, I., Wray, L.V., Jr., and Hillen, W. (1988). Differential regulation of the Tn10 encoded
tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2. EMBO J. 7,
567-572.
van de Löcht, U., Meier, I., Hahlbrock, K., and Somssich, I. E. (1990). A 125 bp promoter fragment is
sufficient for strong elicitor-mediated gene activation in parsley. EMBO J. 9, 2945-2950.
Meier, I., Hahlbrock, K., and Somssich, I. E. (1991). Elicitor-inducible and constitutive in vivo DNA
footprints indicate novel cis-acting elements in the promoter of a parsley gene encoding pathogenesisrelated protein 1. Plant Cell 3, 309-315.
Meier, I., Callan, K. L., Fleming, A. J., and Gruissem, W. (1995). Organ-specific differential regulation
of a promoter subfamily for the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit genes
in tomato. Plant Physiol. 107, 1105-1118.
Meier, I., Phelan, T., Gruissem, W., Spiker, S., and Schneider, D. (1996). MFP1, a novel plant
filament-like protein with affinity for matrix attachment region DNA. Plant Cell 8, 2105-2115.
Rose, A., Meier, I., and Wienand, U. (1999). The tomato I-box binding factor LeMYBI is a member of
a novel class of Myb-like proteins. Plant J. 20, 641-652.
Gindullis, F., and Meier, I. (1999). Matrix Attachment Region Binding Protein MFP1 Is Localized in
Discrete Domains at the Nuclear Envelope. Plant Cell 11, 1117-1128.
Gindullis, F., Peffer, N.A., and Meier I. (1999). MAF1, a novel plant protein interacting with MARbinding protein MFP1 is located at the nuclear envelope. Plant Cell 11, 1755-1767.
Rose, A. and Meier, I. (2001) A domain unique to plant RanGAP is responsible for its targeting to the
plant nuclear rim. Proc. Natl. Acad. Sci. U.S.A. 98, 15377-15382.
Calikowski, T.T., Meulia, T., and Meier, I. (2003) A Proteomic Study of the Arabidopsis Nuclear
Matrix. J. Cell. Biochem. 90, 361-378.
Rose, A., Manikantan, S., Schraegle, S., Maloy, M., Stahlberg, E. and Meier, I. (2004) Genome-wide
Identification of Arabidopsis Coiled-coil Proteins and Establishment of the ARABI-COIL Database.
Plant Physiol. 134: 927-939.
Patel S, Rose A, Meulia T, Dixit R, Cyr RJ, Meier, I. (2004). Arabidopsis WPP-Domain Proteins Are
Developmentally Associated with the Nuclear Envelope and Promote Cell Division. Plant Cell
16:3260-3273.
Jeong, SY, Rose S, Joseph, J, Dasso, M, and Meier, I. (2005). Plant-specific mitotic targeting of
RanGAP requires a functional WPP domain. Plant J 42:270-82.
Rose A, Schraegle SJ, Stahlberg EA and Meier, I (2005). Coiled-coil protein composition of 22
proteomes - differences and common themes in subcellular infrastructure and traffic control. BMC
Evol. Biol 5:66.
Xu X, Rose A, Muthuswamy S, Jeong S-Y, Venkatakrishnan S, Zhao Q, and Meier I. (2007).
NUCLEAR PORE ANCHOR, the Arabidopsis Homolog of Tpr/Mlp1/Mlp2/Megator, Is Involved in
mRNA Export and SUMO Homeostasis and Affects Diverse Aspects of Plant Development. Plant
Cell 19: 1537-1548.
Xu X, Meulia T and Meier I. (2007). Anchorage of Plant RanGAP to the Nuclear Envelope Involves
Novel Nuclear-Pore-Associated Proteins. Curr Biol. 17: 1157-1163.
Zhao, Q, Brkljacic, J, and Meier, I. (2008) Two distinct, interacting classes of nuclear envelope-
associated coiled-coil proteins are required for the tissue-specific nuclear envelope targeting of
Arabidopsis RanGAP. Plant Cell 20, 1639-1651.
18. Xu, X, Zhao, Q, Rodrigo-Peiris, T, Brkljacic, J, He, C, Mueller, S and Meier I. (2008) RanGAP1 is a
continuous marker of the Arabidopsis cell division plane. Proc. Natl. Acad. Sci. USA, in press.
19. Xu XM and Meier I. (2008). The Nuclear Pore Comes to the Fore. Trends in Plant Sci. 13, 1-50.
20. Brkljacic J and Meier I (2008). The nuclear pore and plant development. Curr. Opin. Plant Biol., in
press.
C. Research Support
Ongoing Research Support
04/07 – 03/10 National Science Foundation -Molecular and Cellular Biosciences -0641271 “Arabidopsis
as a new experimental platform to investigate the function of the nuclear pore protein Tpr in
SUMOylation and mRNA export.”
Principle investigator. The objective of this grant is the functional investigation of the plant
nucleoporin NUA/Tpr and how it relates to the connection between SUMOylation and nuclear
RNA export.
Completed Research Support
04/08-03/09 National Science Foundation Molecular and Cellular Biosciences - 0805009 Group
travel proposal: Nuclear Envelope and Golgi, Marseille 2008. Principle investigator. The
purpose was to bring together US and European researchers to discuss connections between the
nuclear envelope and Golgi in plants.
05/04 - 04/08 National Science Foundation -Molecular and Cellular Biosciences -0343167 "Investigating
Structure, Function, and Evolution of a Plant-Specific Nuclear-Targeting Domain"
Principle investigator. The overall goal of this grant was to understand the molecular mechanism
of subcellular anchoring of plant RanGAP and how it relates to the functions of the Ran cycle in
plant interphase and mitosis.
09/02 – 08/06 National Science Foundation - Molecular and Cellular Biosciences- 0209339:
“Arabidopsis 2010: Investigating coiled-coil proteins in the Arabidopsis ORFeome”
Principle investigator. The purpose of this project was to computationally identify all long
coiled-coil proteins in Arabidopsis and functionally investigate a subgroup associated with the
nucleus.
09/01 - 08/05 USDA Plant Growth and Development: “Anchoring of Ran-mediated signal transduction in
plants”. Principle Investigator. The purpose of this research was to identify the unique aspects
of RanGAP subcellular localization in plants.
09/01 - 08/05 National Science Foundation - Molecular and Cellular Biosciences 0079507: “Investigating
the role of novel nuclear envelope-associated plant proteins in nuclear dynamics” The main goal
of this work was to characterize a plant-unique mechanism of nuclear envelope targeting and
identify proteins involved in the mechanism.
Tea Meulia
Molecular and Cellular Imaging Center
The Ohio State University-Ohio Agricultural Research and Development Center
Wooster, OH 44691
Tel: 330-263-3836
FAX: 330-202-3563
email: [email protected]
Professional Preparation
University of Lausanne, Switzerland
Swiss Institute for Experimental Cancer Research, Switzerland
Fred Hutchinson Cancer Research Center, Seattle, WA.
OARDC Ohio State University
Biology/Biochemistry
Genetics
Genetics
Plant Pathology
BS/MS 1985
Ph.D. 1990
PostDoc 1990-94
PostDoc 1995-98
Appointments
1999-date
1998-1999
1995-1998
1990-1994
1985-1990
1983-1986
Research Scientist and Director of the Molecular and Cellular Imaging Center
(http://www.oardc.ohio-state.edu/mcic), a shared technology facility at The Ohio State
University, OARDC, Wooster, OH.
Research Scientist split position in the department of Horticulture and Crop Sciences, with Dr.
David Francis, and the Electron Microscopy Laboratory, The Ohio State University, OARDC,
Wooster, OH.
Research Associate in Plant Pathology with Dr. Donald Gordon, The Ohio State University,
OARDC, Plant Pathology Wooster, OH.
Post-doctoral associate with Dr. Mark Groudine at the Fred Hutchinson Cancer Research
Center, Seattle, WA
High School Teacher at the Liceo Pareto, Leusanne, Switzerland.
Teaching Assistant for genetics courses and laboratory with Prof. Walter Wahli, University of
Lausanne, College of Biology, Lausanne, Switzerland.
Publications in the past five years
Rajakaruna, P., Khandekar, S., Meulia, T., and Leisner, S.M. 2007. Identification and host relations of a
novel comovirus from Ohio. Plant Disease. 91:1212-1220.
Xu, X., Meulia, T. and Meier, I. Anchorage of plant RanGAP to the nuclear envelop involves novel
nuclear-pore-associated proteins. 2007. Current Biology. 17:1157-63.
Fraga, D., Meulia, T. and Fenster, S. Real-time PCR. 2007. Chapter 32 in ‘Current Protocols in Essential
Laboratory Techniques.’ John Wiley & Sons Publishing. In Press.
Welty, N., Radovic, C., Meulia, T., and Van Der Knaap, E. 2007. A developmental analysis of flower and
fruit formation in LA1589, a wild relative of tomato. Canadian Journal of Botany.85:111-118.
Kanneganti, T., Bai, X., Tsai, C., Win, J., Meulia, T., Goodin, M., Kamoun, S., and Hogenhout, S. A. 2007.
A functional genetic assay for nuclear trafficking in plants. The Plant Journal, 50:149-158.
Cheetham, S., Souza, M., McGregor, R., Meulia, T., Wang, Q. and Saif, L.J. Binding patterns of Human
Norovirus-like particles to buccal and intestinal tissues of gnotobiotic pigs in relation to A/H histoblood group antigen expression. 2007. J. Virol. 81: 3535-3544.
Cheetham, S., Souza, M., Meulia, T., Grimes, S., Han, M. G., and Saif, L. J. 2006. Pathogenesis of a
genogroup II human norovirus in gnotobiotic pigs. J. Virol. 80:10372-81.
Radovic, C.; Van Der Knaap, E.; Meulia, T. 2004. Flower development of Lycopersicon pimpinellifolium
LA1589, a close relative of cultivated tomato. Molecular Biology of the Cell 15(Suppl.S) p.217A.
Patel.S., Rose,A. Meulia, T., Dixit, R., Cyr, R.J. and Meier,I. 2004. Arabidopsis WPP-domain proteins are
developmentally associated with the nuclear envelope and promote cell division. The Plant Cell. 16,
3260-3273.
Ammar,E.D., Meulia, T. Özbek E. and Hogenhout,S.A. 2004. Assembly and accumulation sites of maize
mosaic virus (Rhabdoviridae) in plant host and insect vector using transmission electron and
confocal laser microscopy. Book chapter. Current Issues on Multidisciplinary Microscopy Research
and Education. A. Mendez-Vilas and L. Labajos-Broncano [eds.]. Formatex Microscopy Book
Series, no.2, Badajoz, Spain. Pp. 56-64.
Ammar, E.D., Fulton, D., Bai, X., Meulia, T. and Hogenhout, S.A. (2004). An attachment tip and pili-like
structures in insect- and plant-pathogenic spiroplasmas of the class Mollicute. Arch. Microbiol.
181:97-105
Awarded proposals in the past five years
Hogenhout, S.A. Coplin, D.L., and Meulia, T. The nature of the association of Pantoea stewartii, the
causative agent of Stewart’s bacterial wilt of corn, with its flea beetle vector, Chaetocnema
pulicaria. 2006. OARDC Interdisciplinaty Team Research Competition. ($11,000 to the MCIC).
Myers,S., Meulia, T., et al. Equipment and resources for increasing genotyping capabilities at the
(Molecular and Cellular Imaging Center) MCIC for the development of value-added soybean and
other crops for Ohio. Ohio BioProducts Innovation Center. (Wright Centers of Innovation, Third
Frontier grant) - $1,600.000 to the MCIC. 2005 – 2008.
Vanderknaap, E., Kamoun, S., Francis, D., Goodner, B. and Meulia, T. Instrumentation for Highthroughput genotyping and sequencing projects. NSF-MUE. $285.000. June 1st 2004 – May 31st
2005.
Jones, M.L., Meulia, T., and Streeter, J. Role of Metacaspases in petunia petal and leaf senescence.
OARDC Interdisciplinaty Team Research Competition. 2005 - 2006. $100,000.
Finer, J., Ling, P., and Meulia, T. High throughput digital image analysis for promoter analysis in
transiently transformed plant tissues. OARDC - Interdisciplinary Team Research Grant Proposal.
$100,000. 2003 –2005.
Synergistic Activities




In 1995 I was one of the founders of WAMBA (Wooster Area Molecular Biology Association) a
discussion group, which includes plant and animal scientists from the OARDC, and from the
nearby liberal art College of Wooster. This group still meets weekly holding seminars with invited
speakers, or discussions with students, postdocs, faculty on various molecular biology and
genomics topics.
I organize facility tours and science hands on demonstrations for local elementary, middle-high
and high school students, and for students of the nearby Agricultural Technical Institute. In this
year over 300 students toured and participated in demonstrations at the center.
Represent Wooster campus molecular and genomic researchers during State, University or
Industry representatives visits at the OARDC. Some of this year visitors included: Community
representative and politicians: Gordon Gee (OSU president), Joseph Alutto (OSU Provost), Ron
Amstutz (Ohio State Senator), James Carmicheal (Ohio State Representative), Dave Baldwin
(Ohio BioScience Consortium Coordinator), Christine Hazel (Dupont/Pioneer), Brad Moffit (Ohio
Department of Ag.Education), a Delegation of Iraqi agricultural professionals.
Represent the OARDC at public events, such as Plant Discovery Day, BioOhio and the County
Fair.
Thomas K. Mitchell
Fungal Biology and Parasitism Laboratory
Department of Plant Pathology
The Ohio State University
Columbus, OH 43210
(a) Professional Preparation
Pennsylvania State University
Clemson University
North Carolina State University
(b) Appointments
2007-present
2001-2007
1999–2001
Plant Science
Plant Pathology
Plant Pathology
(614) 292-1728
Fax: (614) 292-4455
E-mail: [email protected]
B.S.
M.S.
Ph.D.
1992
1995
2000
Assistant Professor
The Ohio State University, Department of Plant Pathology
Research Assistant Professor
North Carolina State University, Center for Integrated Fungal Research
Senior Research Scientist
North Carolina State University, Fungal Genomics Laboratory
(c) Publications
(i) Related Publications
Oh, Y. Y., Donofrio, N., Pan, H., Coughlan, S., Brown, D., Meng, S., Mitchell, T., and R. Dean (2008).
Transcriptome analysis reveals new insight into appressorium formation and function in the rice blast
fungus, Magnaporthe oryzae. Genome Biology 9:R85.
Craven, K.D., Valez, E., Hicks, R., Cho, Y., Lawrence C.B., and T. K. Mitchell, (2008). The role of
anastomosis in pathogenicity of the fungal pathogen Alternaria brassicicola. Eukaryotic Cell 7:675-683.
Yangre, C., Cramer, R., Kwang-Hyung, K., Davis, J., Mitchell, T., Figuli, P., Prayor, B., Lemasters, E.,
and C. Lawrence, (2007). The Fus3/Kss1 MAP kinase homolog amk1 regulates the expression of several
hydrolytic enzyme genes in the fungus Alternaria brassicicola. Fungal Genetics and Biology, 44:543553.
Torto-Alalibo, T., Tripathy, S., Smith, B.M., Arredondo, F., Zhou, L., Li, H., Qutob, D., Gijzen, M., Mao,
C., Sobral, B.W.S., Waugh, M.E., Mitchell, T.K., Dean, R.A., and B. M. Tyler, (2007). Expressed
sequence tags from Phytophthora sojae reveal genes specific to development and infection. Molecular
Plant-Microbe Interactions, 20:781-793.
Jeong, J., Mitchell, T., and R. Dean, (2007). Characterization of the Magnaporthe grisea snodprot1
homolog, MgSPH1. FEMS Microbiology Letters. 273:157-165.
(ii) Significant Publications
Donofrio, N., Oh, Y., Lundy, R., Pan, H., Jeong, J., Coughlan, S., Mitchell, T., Dean, R. (2006). Gene
expression during nitrogen starvation in the rice blast fungus, Magnaporthe grisea. Fungal Genetics and
Biology, 43:605-617.
Dean, R., Talbot, N., Ebbole, D., Farman, M., Mitchell, T., Orbach, M., Thon, M., Kulkarni, R., Xu, J-R.,
Huaqin, P., Read, N., Lee, Y-H., Carbone, I., Brown, D., Soanes, D., Djonovic, S., Kolomiets, E.,
Rehmeyer, C., Li, W., Harding, M., Lebrun, M-H., Bohnert, H., Kim, S., Galagan, J., Birren, B., (2005).
Analysis of the genome sequence of the plant pathogenic fungus Magnaporthe grisea, the causal agent of
rice blast disease. Nature 434:980-986.
Mitchell, T.K., Alejos-Gonzalez, F., Gracz, H.S., Danehower, D.A., Daub, M.E., Chilton, W.S. (2003).
Xanosporic acid, an intermediate in bacterial degradation of the fungal phototoxin cercosporin.
Phytochemistry 62:723-732.
Mitchell, T.K., Thon, M.R., Jeong, J-S., Brown, D.E., Deng, J., Dean, R.A. (2003). The rice blast
pathosystem as a case study for the development of new tools and raw materials for genome analysis of
fungal plant pathogens. New Phytologist 159: 53-61.
Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R.,
Bidlingmaier, S., Houfek, T., Mitchell, T.K., Miller, P., Dean, R., Gerstein, M., and Snyder, M. (2002).
Global analysis of protein activities using proteome chips. Science 293:2101-2105.
(d) Synergistic Activities
In addition to research activities, my work has extended beyond the laboratory. These extensions include
the participation in the development of extensive database (MGOS) housing the genomic and proteomic
data and bioinformatic tools for the rice blast pathogen M. oryzae. Additionally, I have worked to design
a LIMs system for the high throughput functional analysis of over 50,000 random insertion mutants. My
efforts in the area of outreach and engagement are extensive and varied. Over the past 8 years I
developed a laboratory manual on genetics and genomics and distributed over 40,000 copies, trained over
60 high school teachers in genomics at weeklong workshops, gave talks and laboratory exercises to over
30 K-12 classes, mentored teachers and high school students through research projects, to name a few.
With regard to engagement to minority colleges and students, I have developed course sections and grants
in collaboration with HBCU institutions, mentored faculty on research projects, give dozens of talks and
seminars, and aggressively recruited minority students into the STEM disciplines.
(e) Collaborator and Other Affiliations
(i) Collaborators and co-editors - F. Arredondo Virginia Bioinformatics Institute, B. Birren Broad
Institute, D. Brown North Carolina State University, I. Carbone North Carolina State University, Y. Cho
University of Hawaii, R. Cramer Montana State University, S. Coughlan, Agilent, N. Donofrio
University of Delaware, D. Ebbole Texas A&M University, M. Farman University of Kentucky, J.
Galagan Broad Institute, M. Gijzen Wageningen University Netherland, L. Kohn University of Toronto,
C.B. Lawrence Virginia Bioinformatics Institute, M-H. Lebrun Beyer Crop Science France, Y-H. Lee
Seoul National University Korea , H. Li Virginia Bioinformatics Institute, L. J. Ma Broad Institute, S.
Marek Oklahoma State University, C. Mao Virginia Bioinformatics Institute, D. Okabara University of
Washington, M. Orbach University of Arizona, B. Prayor University of Arizona, D. Qutob Virginia
Bioinformatics Institute, N. Read University of Edinburgh Scotland, B.M. Smith Virginia Bioinformatics
Institute, B.W.S. Sobral Virginia Bioinformatics Institute, C. Soderlund University of Arizona, N. Talbot
University of Exeter UK, M. Thon Salamanca University Spain, T. Torto-Alalibo Virginia Bioinformatics
Institute, S. Tripathy Virginia Bioinformatics Institute, B. M. Tyler Virginia Bioinformatics Institute, B.
Valent Kansas State University, N. Walker Oklahoma State University, G.L. Wang Ohio State University,
J.R. Xu Purdue University, Y. Yang Penn State University, L. Zhou Virginia Bioinformatics Institute, h.
Zhu John’s Hopkins School of Medicine
(ii) Graduate advisors - M. Daub North Carolina State University, R. Dean North Carolina State
University, G. Payne North Carolina State University
(iii) Thesis and postdoctoral advisees - K. Craven Nobel Foundation, E. Valez North Carolina State
University, S. Kim Ohio State University
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Randy Scholl
Associate Professor, Interim Chair
eRA COMMONS USER NAME
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
University of Illinois
North Carolina State Univ.
North Carolina State Univ.
DEGREE
(if applicable)
YEAR(s)
B.S.
M.Sc.
Ph.D.
1968
1970
1972
FIELD OF STUDY
Agricultural Science
Crop Science
Crop Science
A. Positions and Honors.
Positions and Employment
1968-1972
Research Associate, North Carolina State Univ., Raleigh
1972-1973
Post-doctoral Associate, Univ. of Illinois, Urbana
1972-1978
Assistant Professor, Dept. of Genetics, The Ohio State Univ., Columbus
1978-1987
Associate Professor, Dept. of Genetics, The Ohio State Univ., Columbus
1987-1998
Associate Professor, Dept. of Molecular Genetics, The Ohio State Univ., Columbus
1998-present Associate Professor, Dept. of PCMB, The Ohio State Univ., Columbus
Other Experience and Professional Memberships
1986
Visiting Faculty, DOE Plant Res. Lab., Michigan State Univ., East Lansing
1990-present Director, Arabidopsis Biological Resource Center, Ohio State
2001-2006
Executive Committee, TAIR database
2006-present Interim Chair, Dept. of Plant Cellular & Molecular Biology, Ohio State Univ.
B. Selected peer-reviewed publications (in chronological order).
Scholl, R. L., J. E. Harper and R. H. Hageman. 1974. Improvement of the nitrate color development in assays of
nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol. 53:825-828.
Scholl, R. L. 1974. The inheritance of isocitratase activity and its relationship to seedling vigor in a cross of
upland cotton (Gossypium hirsutum L.). Crop Sci. 14:296-300.
Scholl, R. L. 1976. Variability within Gossypium hirsutum L. for seedling isocitratase activity.
Crop Sci. 16:710-703.
Scholl R. L. and P. A. Miller. 1976. Genetic association between yield and lint quality in cotton. Crop Sci.
16:780-783.
Amos, J. A. and R. L. Scholl. 1977. Effect of growth temperature on leaf nitrate reductase, glutamine synthetase,
and NADH-glutatamate dehydrogenase of juvenile maize genotypes. Crop Sci. 17: 445-448.
Scholl, R. L. and K. M. Dennison. 1978. Sensitivity of cultured tissue of Arabidopsis thaliana races to
sulfanilamde. Physiol. Plantar. 43:321-325.
Amos, J. A. and R. L. Scholl. 1978. Induction of haploid callus from anthers of four species of Arabidopsis. Z.
Ppflanzenphysiol. 90:33-43.
Sharma, R. K., B. Griffing and R. L. Scholl. 1979. Variation among Arabidopsis thaliana L. (Heynh.) races for
survival in limited carbon dioxide. Theor. Appl. Genet. 54: 11-15.
Scholl, R. L. and J. A. Amos. 1980. Isolation of doubled haploid plants through anther culture in Arabidopsis
thaliana. Z. Pflanzenphysiol. 96:407-414.
Scholl, R. L., D. E. Keathley and T. J. Baribault. 1981. Enhancement of root formation and fertility in shoots
regenerated from anther- and seedling-derived callus cultures of Arabidopsis thaliana. Z. Pflanzenphysiol.
104:225-231.
Keathley, D. E. and R. L. Scholl. 1982. Culture of Arabidopsis thaliana anthers on liquid medium. Z.
Pflanzenphysiol. 106:199-212.
Keathley, D. E. and R. L. Scholl. 1983. Chromosomal heterogeneity of Arabidopsis thaliana anther callus,
regenerated shoots and plants. Z. Pflanzenphysiol. 112:247-255.
Wang, X. -M., R. L. Scholl and K. A. Feldmann. 1986. Characterization of a chlorate-hypersensitive, high
nitrate reductase Arabidopsis thaliana mutant. Theor. Appl. Genet. 72:328-336.
Zhang, H., R. L. Scholl and C. Somerville. 1988. Double stranded DNA sequencing as a choice for DNA
sequencing. Nucleic Acids Res. 16: 1220.
Wang, X., K. A. Feldmann, and R. L. Scholl. 1988. A chlorate-hypersensitive, high nitrate; chlorate uptake
mutant of Arabidopsis thaliana. Physiol. Plantar. 73: 305-310.
Kim, Y., H. Zhang and R.L. Scholl. 1990. Two evolutionarily divergent genes encode a cytoplasmic ribosomal
protein of Arabidopsis thaliana. Gene 92:177-182.
You T. H., and Scholl, R. L. 1998. PCR amplification of cDNA Libraries forCloning and Screening.
BioTechniques 24:574-575.
Garcia-Hernandez M, Berardini TZ, Chen G, Crist D, Doyle A, Huala E, Knee E, Lambrecht M, Miller N, Mueller
LA, Mundodi S, Reiser L, Rhee SY, Scholl R, Tacklind J, Weems DC, Wu Y, Xu I, Yoo D, Yoon J, Zhang P.
2002. TAIR: a resource for integrated Arabidopsis data. Funct Integr Genomics. Nov; 2(6): 239-53.
Griffing, B. and R.L. Scholl. l991. Qualitative and quantitative studies of
Arabidopsis thaliana. Genetics 129:605-609.
Scholl, R. L, Ware D. H. and May, S. 2000. Seed and Molecular Resources for Arabidopsis. Plant Physiol. 2000
124: 1477-1480.
Meinke, D. and Scholl. R. 2003. The Preservation of Plant Genetic Resources. Experiences with Arabidopsis.
Plant Physiol. 133: 1046-1050.
Rivero-Lepinckas, Luz, Crist, Deborah, Scholl, Randy 2006. Growth of plants and preservation of seeds.
METHODS IN MOLECULAR BIOLOGY 323:3-12.
C. Research Support
Ongoing Research Support
DBI-0542034 Scholl (PI)
5/01/06-3/31/2011
NSF
The Arabidopsis Biological Resource Center at The Ohio State University ($2,940,000)
Role: PI
Completed Research Support
DBI-0091471-suppl. Scholl (PI)
8/2003-3/2006
NSF
The Arabidopsis Biological Resource Center at The Ohio State University ($240,000)
Role: PI
DBI-0091471
Scholl (PI)
4/2001-3/2006
NSF
The Arabidopsis Biological Resource Center at The Ohio State University ($2,790,000)
Role: PI
IBN-9600964-suppl. Scholl (PI)
2/2000-2/2001
NSF
The Arabidopsis Biological Resource Center at The Ohio State University ($50,000)
Role: PI
IBN-9600964
Scholl (PI)
3/1997-2/2001
NSF
The Arabidopsis Biological Resource Center at The Ohio State University ($1,856,000)
Role: PI
BIR-9113224
Scholl (PI)
9/1996-1/1997
NSF - extension
The Arabidopsis Biological Resource Center at The Ohio State University ($179,000)
Role: PI
BIR-9113224
Scholl (PI)
9/1991-8/1996
NSF
The Arabidopsis Biological Resource Center at The Ohio State University ($1,940,000)
Role: PI
? Scholl (PI)
NSF
Anther Culture in Arabidopsis ($58,000)
1979-82
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
David E. Somers
Associate Professor
eRA COMMONS USER NAME
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
Ohio University, Athens, OH
University of Alberta, Edmonton, Alberta Canada.
University of California at Berkeley, Berkeley
The Scripps Research Institute, La Jolla, CA
DEGREE
(if applicable)
YEAR(s)
FIELD OF STUDY
B.Sc.
M.Sc
Ph.D.
1973-1977
Botany
1977-1981
Physiological Plant Ecology
1987-1994
Plant Biology
Post-doctoral
1994-1999
Plant Mol. Genetics
A. Positions and Honors.
Positions and Employment
1977-1980
Graduate Teaching Assistant. Dept. of Botany, University of Alberta, Edmonton, Alberta,
Canada.
1981-1982
Research Assistant, Dept. of Botany, University of Washington, Seattle, WA.
1985-1987
Research Technician, Howard Hughes Medical Institute. University of Washington, Seattle, WA.
1987-1994
Graduate Research Assistant, Dept. of Plant Biology, UC-Berkeley, Berkeley, CA. Peter Quail,
advisor.
1994-1999
Postdoctoral Research Fellow, Dept. of Cell Biology, The Scripps Research Institute, La Jolla,
CA. Steve A. Kay, advisor.
1999-2005
Assistant Professor, Department of Plant Biology, The Ohio State University, Columbus OH
2005-present Associate Professor, Department of Plant Biology, The Ohio State University, Columbus OH
Other Experience and Professional Memberships
Member: Society for Research on Biological Rhythms
Member: American Society of Plant Biologists
Grant review panels
2005
National Science Foundation, Integrative Plant Biology Panel
2005-2006
National Institute of Health, CSD Study Section
2006
National Institute of Health, NRSA Cell Biology and Development (F05) Study Section
2007-2008
National Institute of Health, Neurogenesis and Cell Fate Study Section, Cellular Signaling and
Regulatory Systems
Ad hoc reviews
2000-present
NSF, USDA, BBSRC (UK), BSF (US-Israel Binational Science Foundation), Marsden Fund
(The Royal Society of New Zealand)
Journal Peer review
Science, Nature, PNAS, Current Biology, Nature Reviews Microbiology, PLoS Biology, The Plant Cell, Plant
Journal, Plant Physiology, Plant Molecular Biology, BMC Plant Biology , Planta, Cell Research, Journal of
Biological Rhythms, Plant and Cell Physiology, Philos Trans R Soc Lond B Biol Sci., Plant Cell and
Environment, Photochemistry and Photobiology, Journal of Phycology
1
Honors
1997
1994-1997
JSPS Short-Term Invitation Fellow, Hitachi Advanced Research Lab, Saitama, Japan.
NSF Postdoctoral Research Fellow in Plant Biology
B. Selected peer-reviewed publications (in chronological order; selected from 30 peerreviewed publications).
1. Somers, D.E., Sharrock, R.A., Tepperman, J.T., and Quail, P.H. 1991. The hy3 long
hypocotyl mutant of Arabidopsis is deficient in phytochrome B. Plant Cell 3:1263-1274.
2. Devlin, P. F., Rood, S., Somers, D.E., Quail, P.H., and Whitelam, G.C. 1992.
Photophysiology of the elongated internode (ein) mutant of Brassica rapa: The ein mutant lacks a
detectable phytochrome B-like polypeptide. Plant Physiol 100:1442-1447.
3. Wester, L., Somers, D.E., Clack, T. and Sharrock, R.A. 1993. Transgenic complementation of the
hy3 phytochrome B mutation and PHYB gene copy number effects in Arabidopsis. Plant J. 5:261-272
4. Dehesh, K., Franci, C., Sharrock, R.A., Somers, D.E., Welsh J. and Quail, P.H. 1994. The Arabidopsis
phytochrome A gene has multiple transcription start sites and a promoter sequence motif homologous to the
repressor element of monocot phytochrome A genes. Photochem. Photobiol. 59: 379-384.
5. Somers, D.E. and Quail, P.H. 1995. Phytochrome-mediated light regulation of PHYA- and PHYB-GUS
transgenes in Arabidopsis thaliana seedlings. Plant Physiol 107:523-534.
6. Somers, D.E. and Quail, P.H. 1995. Temporal and spatial expression patterns of PHYA and PHYB genes in
Arabidopsis. Plant J. 7:413-427
7. Hicks, K.A., Millar, A.J., Carre, I.A., Somers, D.E., Straume, M., Meeks-Wagner, D.R. and
Kay, S.A. 1996. Conditional circadian dysfunction of the Arabidopsis early-flowering 3 mutant.
Science 274: 790-792.
8. Anderson, S.L., Somers, D.E., Millar, A.J., Hanson, K., Chory, J. and Kay, S.A. 1997.
Attenuation of phytochrome A and B signaling pathways by the Arabidopsis circadian clock.
Plant Cell 9:1727-1743.
9. Devlin, P.F., Somers, D.E., Quail, P.H., and Whitelam, G.C. 1997. The Brassica rapa elongated
internode (EIN) gene encodes phytochrome B. Plant Mol. Biol. 34:537-547.
10. Somers, D.E., Devlin, P.A. and Kay, S.A. 1998. Phytochromes and cryptochromes in the
entrainment of the Arabidopsis circadian clock. Science 282:1488-1490.
11. Somers, D.E., Webb, A., Pearson, M., and Kay, S.A. 1998. The short-period mutant, toc1-1,
alters circadian clock regulation of multiple outputs throughout development in Arabidopsis
thaliana. Development 125:485-494.
12. Park D.H.*, Somers D.E.*, Kim Y.S., Choy Y.H., Lim H.K., Soh M.S., Kim H.J., Kay S.A.,
Nam H.G. 1999. Control of circadian rhythms and photoperiodic flowering by the Arabidopsis
GIGANTEA gene. Science 285: 1579-1582. *(equal contributions as first authors).
13. Somers, D.E., Schultz, T.F., Milnamow, M. and Kay S.A. 2000. ZEITLUPE encodes a novel
clock associated PAS protein from Arabidopsis. Cell 101:319-329.
14. Strayer, C., Oyama, T., Schultz, T.F., Raman, R., Somers, D.E., Mas, P., Panda, S., Kreps,
J.A.and Kay, S.A. 2000. Cloning of the Arabidopsis clock gene TOC1, an autoregulatory
response regulator homolog. Science 289:768-771.
15. Kim, W-Y.*, Geng, R.* and Somers, D.E. 2003. Circadian phase-specific degradation of the Fbox protein ZTL is mediated by the proteasome. Proc Natl Acad Sci U S A 100:4933-4938.
*(equal contributions as first authors).
16. Risseeuw, E.P., Daskalchuk, T.E., Banks, T.W., Liu, E., Cotelesage, J., Hellman, H., Estelle,
M., Somers, D.E., and Crosby, W.L. 2003. Protein interaction analysis of SCF ubiquitin E3
ligase subunits from Arabidopsis. Plant J. 34:753-767.
17. Mas, P., Kim, W-Y., Somers D.E., and Kay S.A. 2003. Targeted degradation of TOC1 by ZTL
modulates circadian function in Arabidopsis thaliana. Nature 426:567-70.
18. Somers, D.E., Kim, W-Y., Geng, R. 2004. Dosage-dependent control of circadian period
2
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20.
21.
22.
23.
24.
25.
26.
and photomorphogenesis by the F-box protein ZEITLUPE. Plant Cell 16:769-782.
Han, L., Mason, M, Risseeuw, E.P., Crosby, W.L. and Somers, D.E. 2004. Formation of an
SCFZTL complex is required for proper regulation of circadian timing. Plant J. 40:291-301.
Kim, W.Y., Hicks, K. A. and Somers, D.E. 2005. Independent roles for EARLY FLOWERING 3 and
ZEITLUPE in the control of circadian timing, hypocotyl length, and flowering time. Plant Physiol. 139:
1557-69.
Kevei, E., Gyula, P., Hall, A., Kozma-Bognar, L., Kim, W.Y., Eriksson, M.E., Toth, R.,
Hanano, S., Feher, B., Southern, M.M., Bastow, R.M., Viczian, A., Hibberd, V., Davis, S.J.,
Somers, D.E., Nagy, F., and Millar, A.J. 2006. Forward genetic analysis of the circadian clock separates
the multiple functions of ZEITLUPE. Plant Physiol. 140:933-945.
Allen T., Koustenis A., Theodorou G., Somers, D.E., Kay S.A., Whitelam G.C., Devlin
P.F. 2006. Arabidopsis FHY3 specifically gates phytochrome signaling to the circadian
clock. Plant Cell 18:2506-2516
Kim,W.Y.*, Fujiwara, S.*, Suh, S.S., Kim, J., Kim, Y., Han, L., David, K., Putterill, J.,
Nam, H.G., and Somers, D.E. 2007. ZEITLUPE is a circadian photoreceptor stabilized by
GIGANTEA in blue light. Nature 449:356-360. *(equal contributions as first authors).
Jin JB, Jin YH, Lee J, Miura K, Yoo CY, Kim WY, Van Oosten M, Hyun Y, Somers DE,
Lee I, Yun DJ, Bressan RA, Hasegawa PM. 2008. The SUMO E3 ligase, AtSIZ1, regulates
flowering by controlling a salicylic acid-mediated floral promotion pathway and through effects on FLC
chromatin structure. Plant J. 3:530-40
Fujiwara, S.*, Wang, L.*, Han, L., Suh, S. S., Salome, P. A., McClung, C. R., Somers, D.E.
2008. Post-translational regulation of the Arabidopsis circadian clock through selective
proteolysis and phosphorylation of pseudo- response regulator proteins. J. Biol. Chem.
283: 23073-23083. *(equal contributions as first authors).
Somers, D. E. and Fujiwara, S. 2009. Thinking outside the F-box: novel ligands for novel
receptors. Trends Plant Sci. (in submission).
C. Research Support
Ongoing Research Support
NSF Molecular and Cellular Biosciences
05/01/2006 - 04/30/2009
MCB-0544137
Somers (PI)
Functional Domains and Spatial-Temporal Interactions of an Arabidopsis Circadian Clock-Associated
F-Box Protein”.
This study focuses on the functional dissection of ZTL, its intracellular site of action, and identifies additional
biochemical interactors.
NSF Integrative Organismal Biology
02/01/2008 - 01/31/2011
IOS-0748749
Somers (PI)
The control of reproductive onset and the circadian clock by GIGANTEA.
This project defines the biochemical function of GI by identifying which domains are effective for the
interaction and stabilization of ZTL and identifies other GI interactors.
Completed Research Support
NSF Molecular and Cellular Biosciences
04/01/2001 - 03/31/2004
MCB-0080090
Somers (PI)
Molecular and genetic analysis of ZTL function in the Arabidopsis circadian clock”.
This work positioned the ZTL locus within the context of the known red light and blue light signal transduction
pathways and identified physical interaction partners of the ZTL protein.
3
CSREES/USDA Plant Growth and Development
09/01/2002 - 08/31/2004
2002-35304-12594 Somers (PI)
Functional in planta analyses of a clock-associated F-box protein.
The goals of this project was to identify and confirm the functional domains of ZTL.
NSF Integrative and Organismal Biology:
04/01/2004 - 03/31/2007
IBN-0344377
Somers (PI)
Genetic and molecular interactors in circadian clock function.
The major goals of this project were to identify genetic suppressors and enhancers of the circadian clock
component ZEITLUPE.
NSF Biological Infrastructure
05/01/2005 - 04/30/2008
DBI-0500601
Somers (lead PI)
High-resolution CCD camera and luminometer for high-throughput luciferase-based promoter analysis.
This award supported the purchase of two systems used to localize and measure luciferase activity resulting
from expression of chimeric reporter genes that consist of the luciferase gene fused to a promoter region of the
genome. One system is a high throughput luminometer and the other is based on a low-light CCD camera.
4
BIOGRAPHICAL SKETCH
Eric J. Stockinger
Department of Horticulture and Crop Science
The Ohio State University/OARDC
Wooster OH 44691
Professional Preparation
University of California, Riverside CA
University of California, Riverside CA
Michigan State University, East Lansing MI
Michigan State University, East Lansing MI
Plant Science
Botany (Plant Genetics)
Plant Genetics
Gene Regulation
B.S.
Ph.D.
Postdoc
Postdoc
1985
1993
1993-1994
1994-2000
Appointments
2006-Present Associate Professor, Dept of Horticulture and Crop Science. The Ohio State University/OARDC,
Wooster OH 44691
2000-2006 Assistant Professor, Dept of Horticulture and Crop Science. The Ohio State University/OARDC,
Wooster OH 44691
1994-2000 Postdoctoral Research Assistant, Dept of Crop & Soil Sciences, Michigan State University, East
Lansing MI; Research Advisor: Michael F. Thomashow
1993-1994 Postdoctoral Research Assistant, Dept of Horticulture, Michigan State University, East Lansing
MI; Research Advisor: Amy F. Iezzoni
1986-1993 Research Assistant, Dept of Botany & Plant Sciences, University of California Riverside CA;
Research Advisor: Linda L. Walling
1990
Teaching Assistant (Molecular Biology), Dept of Biology, University of California, Riverside
CA; Teaching Advisor: Brad Hyman
1986
Teaching Assistant (Genetics), Dept of Biology, University of California, Riverside CA;
Teaching Advisor: Gary Jones
1984-1986 Undergraduate Research Assistant, Dept of Botany & Plant Sciences, University of California,
Riverside CA; Research Advisor: J. Giles Waines
Publications:
Pennycooke, J.C., Cheng, H., Roberts, S.M., Yang, Q., Rhee, S.Y., and Stockinger, E.J. (2008) The low
temperature-responsive, Solanum CBF1 genes maintain high identity in their upstream regions in a
genomic environment undergoing gene duplications, deletions, and rearrangements. Plant Mol. Biol. 67,
483-497.
Pennycooke, J.C., Cheng, H. and Stockinger, E.J. (2008) Comparative genomic sequence and expression
analyses of Medicago truncatula and alfalfa subspecies falcata COLD-ACCLIMATION-SPECIFIC genes.
Plant Physiol. 146, 1242-1254.
Knox, A.K., Li, C., Vagujfalvi, A., Galiba, G., Stockinger, E.J., and Dubcovsky, J. (2008) Identification of
candidate CBF genes for the frost tolerance locus Fr-Am2 in Triticum monococcum. Plant Mol Biol. 67,
257-270.
Xiao, H., Jiang, N., Schaffner, E., Stockinger, E.J., and van der Knaap, E. (2008) A retrotransposonmediated gene duplication underlies morphological variation of tomato fruit. Science 319, 1527-1530.
Stockinger, E.J., Skinner, J.S., Gardner, K.G., Francia, E., and Pecchioni, N. (2007) Expression levels of
barley Cbf genes at Frost resistance-H2 are dependent upon alleles at Fr-H1 and Fr-H2. Plant J. 51:308321.
Stockinger, E.J., Cheng, H., and Skinner, J.S. (2005) Structural organization of barley CBF genes coincident
with a QTL for cold hardiness. In, “Cold Hardiness in Plants,” THH Chen, M Uemura and S Fujikawa, (eds.),
CABI Publishing, Wallingford, Oxon, UK. pp. 53-63.
Wang, Z., Triezenberg, S.J., Thomashow, M.F., and Stockinger E.J. (2005) Multiple hydrophobic motifs in
Arabidopsis CBF1 COOH-terminus provide functional redundancy in trans-activation. Plant Mol. Biol.
58:543-559.
Stockinger, E.J., Mao, Y., Regier, M., Triezenberg, S.J. and Thomashow, M.F. (2001) Transcriptional
Adaptor and Histone Acetyltransferase Proteins in Arabidopsis and their Interactions with CBF1, a
Transcriptional Activator Involved in Cold-Regulated Gene Expression. Nucleic Acids Res. 29:1524-1533.
Stockinger, E.J., Gilmour, S.J. and Thomashow, M.F. (1997) Arabidopsis thaliana CBF1 encodes an AP2
domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory
element that stimulates transcription in response to low temperature and water deficit. Proc. Natl. Acad.
Sci. U.S.A. 94:1035-1040.
Manuscripts Submitted:
Knox, A.K., Cheng, Hongmei, Tondelli, A., Dhillon, T., Nelson, C.W., Welch, L.R., Pecchioni, N., and
Stockinger, E.J. (Submitted) The barley (Hordeum vulgare) CBF2A-CBF4B genomic region at Frost
Resistance-H2 exists as a copy number variant locus in which multiple gene copies typify cultivated
genotypes possessing vrn-H1 winter alleles
Synergistic Activities
• Established summer internship program bringing students from underrepresented groups to the Stockinger
lab (seven students 2002 to 2005); training of eight additional undergraduate students in research
• Co-instructor of Agricultural genomics; Courses developed: 1) Yeast One-Hybrid, a laboratory exercise;
2)Transcription factors controlling plant traits of agroeconomic value; 3) Molecular Genetic Control of
Flowering: Integration of Environmental Signals with the Endogenous Program of Plant Development
• Developed online handbook of molecular biology protocols (http://www.oardc.ohiostate.edu/stockingerlab/)
• Created bacteriophage λ genomic libraries (available upon request) in 16 plant genotypes and species
• Journal peer review service (40 articles 2000-2008; Biochim. Biophys. Acta., Genetics, J. Biol. Chem., Int.
J. Biochem. Cell Biol., J. Am Soc. Hort Sci., J. Exp Bot., Mol. Genet. Genomics, Nucleic Acids Res., Plant
Cell, Plant J., J. Plant Res., Plant Breeding, Plant Mol. Biol., Plant Physiol., Proc. Natl. Acad. Sci.,
U.S.A., Science)
• Grant review service (Ad hoc reviewer 12 proposals 2000-2008; NSF, USDA NRI, U.S. Israel BARD, and
internal, and Panel Member 2006 USDA NRI
Biographical Sketch
F. Robert Tabita, Principal Investigator
Education: B.S. in Biology/Chemistry (1961-65) and M.S. in Microbiology (1965-67), St. John’s
University, Jamaica, N. Y.; Ph.D. in Microbiology/Biochemistry (1967-71), Syracuse University
(with D.G. Lundgren); NIH Postdoctoral Fellow in Department of Chemistry (1971-73),
Washington State University (with B. A. McFadden).
Positions: Professional Experience: 1973-1988, Assistant, Associate, and Professor,
Department of Microbiology, University of Texas at Austin; 1985, Visiting Professor, Summer
program at Gray Fresh-water Res. Inst., Univ. of Minnesota; 1981-1988, Associate Director,
Center for Applied Microbiology, University of Texas at Austin; 1987, Sabbatical in protein
engineering at Genencor/Genentech, Inc., South San Francisco; 1989-Present, Ohio Eminent
Scholar of Microbiology; Professor, Dept. of Microbiology, Prof. Plant Cellular and Molecular
Biology Dept., Prof. Natural Resources, The Ohio State University; member of interdisciplinary
Biochemistry and Molecular Biology programs; Chair, OSU Provost's Initiative in the Molecular
Life Sciences (1997-2002); Currently Director, OSU Plant Molecular Biology/Biotechnology
Program and Plant Biotechnology Center; Director, OSU Plant-Microbe Genomics Facility
Honors and Professional Service: 1961-65, N.Y. State Regents Scholarship (undergraduate);
1971-73, NIH Postdoct. Fellowship; 1976-78, Member NIH Biochemistry Study Section; 197984, Member Ed. Bd. J. Bacteriology; 1981, Member USDA Biological N2 Fixation Review Panel;
1984, Member of USDA Photosynthesis Panel; 1984-88, Member of NIH Microbial Physiology &
Genetics Study Section (subcom.1); 1986, Co Editor, FEMS Microbiol. Rev. Special Edition on
C1 Metabolism; 1989, Appointed Visiting Prof. and external Ph.D. examiner (opponent) at
Uppsala Univ.; 1990, Co-Editor, Advances in Microbial Autotrophy and One-Carbon Metabolism
(vol. 1) Kluwer Acad. Pub.; 1992, Member NIH Biochemistry Study Section for AREA Grants;
1993, Member Special NIH Study Section to evaluate grants of study section members; 1994,
Elected Fellow of the Amer. Acad. Microbiol.; 1994-1996, Alt. Councilor, then Councilor,
Microbial Physiol.& Metab. Section of Amer. Soc. Microbiol.; 1994, Chosen to nominate Nobel
Prize in Chemistry by Nobel Comm.; 1995-2000, Editor, Arch. Microbiol.; 1996, Chair, Special
Study Section for NIH to evaluate grants of study section members; 1996, Member of Eval.
Team for Biology Dept. at Univ. Memphis; 1995, Co-Ed., Microbial Growth on C1 Compounds,
Kluwer, 363 pp.; 1997-1998, Chair and Founder, Gordon Research Conference "Molecular
Basis of Microbial One-Carbon Metabolism"; 1998, Member of External Committee to Evaluate
Functional Genomics/Bioinformatics Res. Center at Virginia Polytechnic Institute and State
University; 2000, DOE Energy Biosciences Grants Review Panel; 2001, DOE Advis. Comm. on
Carbon Sequestration; 2002, Member DOE Genomes to Life Review Panel; 2005, Subpanel
Chair (Biofuels), DOE Solar Energy Conf.; 2005, DOE Energy from Biomass Workshop; 2005 present, Scientific Advisory Comm., Center for Molecular Systems, Oak Ridge National Lab
Publications since 2003 (of a total of 210)
179.
180.
Robinson, J. J., Scott, K. T., Swanson, S. T., O'Leary, M. H., Horken, K., Tabita, F. R,
and Cavanaugh, C. M. Kinetic isotope effect and characterization of form II RubisCO
from the chemoautotrophic endosymbionts of the hydrothermal vent tubeworm Riftia
pachyptila. Limnol. Oceanogr. 48 (2003) 48-54.
Dubbs, J. M., and Tabita, F. R. Interactions of the cbbII promoter-operator region with
CbbR and RegA(PrrA) regulators indicate distinct mechanisms to control expression of
the two cbb operons of Rhodobacter sphaeroides. J. Biol. Chem. 278 (2003) 1644316450.
1
181.
182.
183.
184.
185.
186.
187.
188.
189.
190.
191.
192.
193.
194.
195.
196.
Finn, M. W., and Tabita, F. R. Synthesis of catalytically active form III ribulose 1,5bisphosphate carboxylase/oxygenase in archaea. J. Bacteriol. 185 (2003) 3049-3059.
Hanson, T. E., and Tabita, F. R. Insights into the stress response and sulfur metabolism
revealed by proteome analysis of a Chlorobium tepidum mutant lacking the RubisCOlike protein. Photosynth. Res. 78 (2003) 231-248.
Smith, S. A., and Tabita, F. R. Positive and negative bioselection of mutant forms of
prokaryotic (cyanobacterial) ribulose-1, 5-bisphosphate carboxylase/oxygenase. J. Mol.
Biol. 331 (2003) 557-569.
Tabita, F. R. Research on carbon dioxide fixation in photosynthetic microorganisms
(1971 – present). Photosynth. Res. 80 (2004) 315-332.
Dubbs, J. M., and Tabita, F. R. Regulators of nonsulfur purple phototrophic bacteria and
the interactive control of CO2 assimilation, nitrogen fixation, hydrogen metabolism and
energy generation. FEMS Microbiol. Rev. 28 (2004) 353-376.
Larimer, F.W., Chain, P., Hauser, L., Lamerdin, J., Malfatti, S., Do, L., Land, M.,Pelletier,
D.A., Beatty, J.T., Lang, A.S., Tabita, F. R., Gibson, J.L., Hanson, T. E., Bobst, C.,Torres
y Torres, J., Peres, C., Harrison,F.H., Gibson, J., and Harwood, C.S. Complete genome
sequence of the metabolically versatile photosynthetic bacterium Rhodopseudomonas
palustris. Nature Biotechnology 22 (2004) 55-61. [cover photograph].
Tabita, F. R., and Hanson, T. E. Anoxygenic photosynthetic bacteria. In: Microbial
Genomics (2004) C. M. Fraser, K. E. Nelson, and T. D. Read (eds.). Humana Press,
Inc., Totowa, NJ, pp. 225-243.
Bobst, C. E., and Tabita, F. R. The role of cysteine 160 in thiamine diphosphate binding
of the Calvin-Benson-Bassham cycle transketolase (CbbT) of Rhodobacter sphaeroides.
Arch. Biochem. Biophys. 426 (2004) 43-54. [cover photograph].
Smith, S. A., and Tabita, F. R. Glycine 176 affects catalytic properties and stability of the
Synechococcus sp. strain PCC 6301 ribulose 1,5-bisphosphate carboxylase/oxygenase.
J. Biol. Chem. 279 (2004) 25632-25637.
Finn, M. W., and Tabita, F. R. A modified pathway to synthesize ribulose 1,5bisphosphate in methanogenic archaea. J. Bacteriol. 186 (2004) 6360-6366.
Dubbs, P., Dubbs, J. M., and Tabita, F. R. Effector-mediated interaction of CbbRI and
CbbRII regulators with target sequences in Rhodobacter capsulatus. J. Bacteriol. 186
(2004) 8026-8035.
Li, H., Sawaya, M. R., Tabita, F. R., and Eisenberg, D. Crystal structure of a novel
RuBisCO-like protein from the green sulfur bacterium Chlorobium tepidum. Structure 13
(2005) 779-789.
Dangel, A. W., Gibson, J. L., Janssen, A. P., and Tabita, F. R. Residues that influence in
vivo and in vitro CbbR function in Rhodobacter sphaeroides and identification of a
specific region critical for co-inducer recognition. Mol. Microbiol. 57 (2005) 1397-1414.
Zianni, M.,Tessanne, K., Merighi, M., Laguna, R., and Tabita, F. R. Identification of the
DNA bases of a DNase I footprint by the use of dye primer DNA sequencing on an
automated capillary DNA analysis instrument. J. Biomol. Techniques 17 (2006) 103-113.
Romagnoli, S., and Tabita, F. R. A novel three-protein two-component system provides
a regulatory twist on an established circuit to modulate expression of the cbbI region of
Rhodopseudomonas palustris CGA010. J. Bacteriol. 188 (2006) 2780-2791.
VerBerkmoes, N. C., Shah, M. B., Lankford, P. K., Pelletier, D. A., Strader, M. B., Tabb,
D. L., McDonald, W. H., Barton, J. W., Hurst, G. B., Hauser, L., Davison, B. H., Beatty,
J. T., Harwood, C. S., Tabita, F. R., Hettich, R. L., and Larimer, F. W. Determination and
comparison of the baseline proteomes of the versatile microbe Rhodopseudomonas
palustris under its major metabolic states. J. Proteome Res. 5 (2006) 287-298.
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201.
202.
203.
204.
205.
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207.
208.
209.
210.
John, D. E., Wawrik, B., Tabita, F. R., and Paul, J. H. Gene diversity and organization in
rbcL-containing genome fragments from uncultivated Synechococcus in the Gulf of
Mexico. Mar. Ecol. Prog. Ser. 316 (2006) 23-33.
Tabita, F. R. Research on carbon dioxide fixation in photosynthetic microorganisms
(1971-present) In: Discoveries in Photosynthesis (2006). Govindjee, J. T. Beatty, H.
Gest, and J. F. Allen (eds.). Springer, pp. 771-788.
Kim, W., and Tabita, F. R. Both subunits of ATP-citrate lyase from Chlorobium tepidum
contribute to catalytic activity. J. Bacteriol. 188 (2006) 6544-6552.
Hoeft, S. E., Switzer-Blum, J., Stolz, J. F., Tabita, F. R., Witte, B., King, G. M., Santini, J.
M., and Oremland, R.S. Alcalilimnicola ehrlichei, sp. nov., a novel, arsenite-oxidizing
haloalkaliphilic proteobacterium capable of chemoautotrophic or heterotrophic growth
with nitrate or oxygen as the electron acceptor. Int. J. Syst. & Evol. Microbiol. 57 (2007)
504-512.
Romagnoli, S., and Tabita, F. R. Phosphotransfer reactions of the CbbRRS three-protein
two-component system from Rhodopseudomonas palustris CGA010 appear to be
controlled by an internal molecular switch on the sensor kinase. J. Bacteriol. 189 (2007)
325-335.
Kreel, N. E., and Tabita, F. R. Substitutions at methionine 295 of the Archaeoglobus
fulgidus ribulose-1,5-bisphosphate carboxylase/oxygenase affect oxygen binding and
CO2/O2 specificity. J. Biol. Chem. 282 (2007) 1341-1351.
Tabita, F. R. Rubisco: the enzyme that keeps on giving. Cell 129 (2007) 1039-1040.
Tabita, F. R., Hanson, T. E., Li, H., Satagopan, S., Singh, J., and Chan, S. Function,
structure, and evolution of the RubisCO-like proteins and their RubisCO homologs.
Microbiol. Mol. Biol. Reviews 71 (2007) 576-599.
John, D. E., Wang, Z.A., Liu, X., Byrne, R. H., Corredor, J. E., Lopez, J. M., Cabrera, A.
Bronk, D. A., Tabita. F. R., and Paul, J. H. Phytoplankton carbon fixation gene
(RubisCO) transcripts and air-sea CO2 flux in the Misssisssippi River plume. Int. Soc.
Mic. Ecol. Journal 1 (2007) 517-531.
Tabita, F. R., Satagopan, S., Hanson, T. E, Kreel N. E, and Scott, S. S. Distinct form I, II,
III, and IV Rubisco proteins from the three kingdoms of life provide clues about Rubisco
evolution and structure/function relationships. J. Exp. Bot. 59 (2008) 1515-1524.
Tabita, F. R., Hanson, T. E., Satagopan, S., Witte, B. H., and Kreel N. E. The evolution,
structure, and function of RubisCO and its homolog the RubisCO-like protein. Phil.
Trans. Royal Soc. SerB 363 (2008) 563-576.
Romagnoli, S., and Tabita, F. R. Carbon dioxide metabolism and its regulation in
nonsulfur purple photosynthetic bacteria. In, Hunter C.N., Daldal F., Thurnauer M.C.,
and Beatty J. T. (2008) The Purple Phototrophic Bacteria. Advances in Photosynthesis
and Respiration, Vol. 28, Springer, Dordrecht, The Netherlands, pp. 563-576.
Imker, H. J., Singh, J., Warlick, B. P., Tabita, F. R. and Gerlt, J. A. Mechanistic diversity
in the RuBisCO superfamily: A novel isomerization reaction catalyzed by the
RuBisCO-like protein from Rhodospirillum rubrum. Biochemistry (2008) 11171-11173.
Dangel, A. W., and Tabita, F. R. Protein-protein interactions between CbbR and
Reg(PrrA): transcriptional regulatorsof the cbb operons of Rhodobacter sphaeroides.
Mol. Microbiol. (in press).
Current Support
1.
NIH 5-RO1 GM077625-02
F. R. Tabita, PI at OSU for a joint UCLA-OSU project March 1, 2006-February 2009
5% effort
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“Engineering large scale pathways into Escherichia coli.”
Dr. James C. Liao from UCLA is the PI and this grant was awarded via the Interagency
Metabolic Engineering Initiative (with FRT as CoPI)
The long-term goal is to engineer new pathways into E. coli using genes from
Rhodopseudomonas palustris
2.
DOE DE-FG02-07ER64489
“Bioengineering and coordination of regulatory networks and intracellular complexes to
maximize hydrogen production by phototrophic microorgansims”;
F. R. Tabita, PI; Sept. 15, 2007 – Sept. 2010
The long-term goal is to elucidate the molecular control of interacting systems that impinge on
bio-hydrogen production in nonsulfur purple bacteria
3.
DOE DE-FG02-08ER15976
“Production of biofuels and value-added products via solar and chemical energy driven
bioconversions of CO2 fixing microbes”
F. R. Tabita, PI; Sept. 1, 2008 – August 31, 2011
The long-term goal of this project is to study the molecular control of CO2 fixation genes that
affect the potential to convert CO2 to value-added chemicals
4.
U. S. Air Force
“Optimization of biofuel production from transgenic microalgae”
R. Sayre, Danforth Foundation, PI; F. R. Tabita, CoPI; Sept. 1, 2008 to Aug. 31, 2011
The long term goal of this research is to maximize various biofuel production in algae after
manipulation of molecular control circuits and key enzymes.
5.
OARDC
“Summer workshop in functional genomics”
F. R. Tabita, PI; E. Grotewold, CoPI; Jan. 1, 2008 – Dec. 31, 2009.
The goal is to provide partial support to operate a functional genomics training program for Ohio
students in the area of plant molecular biology and microbiology
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Biographical Sketch
Esther Klazina Maria van der Knaap
Department of Horticulture and Crop Science, The Ohio State University/OARDC,
Wooster, OH 44691; Tel: 330-263-3822; FAX: 330-263-3887; email: [email protected]; web:
http://www.oardc.ohio-state.edu/vanderknaap
PROFESSIONAL PREPARATION
Wageningen University, Wageningen, the Netherlands
Michigan State University, East Lansing MI, USA
Cornell University, Ithaca NY, USA
BS/MS 1990 Plant Pathology
Ph.D 1998 Genetics
PD
1998-2001
PROFESSIONAL EXPERIENCE
2006-present Assistant professor, Department of Horticulture and Crop Science, OARDC/The Ohio
State University, Wooster OH, USA
2001-2006
Research Scientist and Adjunct Assistant Professor, Department of Horticulture and Crop
Science, OARDC/The Ohio State University, Wooster OH, USA.
1990-1992
Visiting researcher, Department of Nematology, University of California, Riverside CA,
USA.
PUBLICATIONS most closely related to the project:
Xiao, H., N. Jiang, E.K. Schaffner, E.J. Stockinger, E. van der Knaap. 2008. A retrotransposon-mediated
gene duplication underlies morphological variation in tomato fruit. Science 319: 1527-1530.
Featured on the cover of the journal
Gonzalo, M.J. and E. van der Knaap 2008 A comparative analysis into the genetic bases of tomato
varieties exhibiting elongated fruit shape. Theor Appl Genet 116: 647-656
Brewer, M.T., J.B. Moyseenko, A.J. Monforte, E. van der Knaap 2007. Morphological Variation in
Tomato: A Comprehensive Study of Quantitative Trait Loci Controlling Fruit Shape and
Development. J Exp Bot 58: 1339-1349
Van Deynze, A., K. Stoffel, C.R. Buell, A. Kozik, J. Liu, E. van der Knaap, D.M. Francis. 2007 Diversity
in conserved genes in tomato. BMC Genomics 8:465.
Brewer, M.T., L. Lang, K. Fujimura, N. Dujmovic, S. Gray, E. van der Knaap 2006. Development of a
controlled vocabulary and software application to analyze fruit shape variation in tomato and
other plant species. Plant Physiol 141: 15-25. Featured on the cover of the journal
Other significant publications:
Olmstead, J.W., A.M. Sebolt, A. Cabrera, S.S. Sooriyapathirana, S. Hammar, G. Iriarte, D. Wang, C.Y.
Chen, E. van der Knaap, A.F. Iezzoni. 2008. Construction of an intra-specific sweet cherry
(Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map. Tree
Genet Genomics 4: 897-910.
Darrigues, A., J. Hall, N. Dujmovic, S. Gray, E. van der Knaap, D.M. Francis. 2008. Tomato Analyzer –
Color Test: a new tool for efficient digital phenotyping. J Am Soc Hort Sci 133: 579-586.
Paran, I. and E. van der Knaap. 2007 Genetic and molecular regulation of fruit and plant domestication
traits in tomato and pepper. J Exp Bot 58: 3841-3852
Tian, M., J. Win, J. Song, R. van der Hoorn, E. van der Knaap and S. Kamoun 2007. A Phytophthora
infestans cystatin-like protein targets a novel tomato papain-like apoplastic protease. Plant
Physiol 143: 364-377.
Welty, N., C. Radovich, T. Meulia, E. van der Knaap 2007. Inflorescence development in two tomato
species. Can J Bot 85: 111-118
SYNERGISTIC ACTIVITIES
• Chair of the Council, Plant Molecular Biology and Biotechnology Program. 2006-2008.
• Organizer, Wooster Area Molecular Biology Association weekly campus-wide seminar series.
2003-2006.
• Hosted 13 summer undergraduate laboratory interns. Hosted Independent Studies projects for
Sarah McNulty (ATI) and Erin Schaffner (CoWooster).
• Computational undergraduate research projects with Dr. Simon Gray and students Nancy
Dujmovic, Ben Strecker, Rick Drushal, Ellen Wagner and David Sullivan (CoWooster). This
project resulted in several papers with undergraduate authors.
• Database construction project with undergraduates Dustin Welty, Traian Andrei, Jason Berry
under supervision of Dr. Denise Byrnes (CoWooster).
COLLABORATORS AND COAUTHORS IN THE LAST 4 YEARS
Alarcon, A (IRTA, Barcelona Spain); Alvarez, JM (IRTA, Barcelona Spain); Arus, Pere (IRTA,
Barcelona Spain); Bai, Xiaodong (Ohio State); Bedinger, Pat (Colorado State); Brewer, Marin (Ohio
State); Buell, Robin (Mich State); Cabrera, Antonio (Ohio State); Chen, C. (Mich State); Chetelat, Roger
(UC-Davis); Darrigues, Audrey (Ohio State); Drushal, Rick (College of Wooster); Dujmovic, Nancy
(College of Wooster); Eaton, Christina (unknown); Eduardo, I (IRTA, Barcelona Spain); FernándezTrujillo, JP (IRTA, Barcelona Spain); Francis, David (Ohio State); Fujimura, Kikuo (Ohio State);
Gonzalo, Maria Jose (Zaragoza, Spain); Goodner, Brad (Hiram College); Gray, Simon (College of
Wooster); Hall, Jack (Ohio State); Hammar, Sue (Mich State); Hogenhout, Saskia (John Innes, UK);
Iezzoni, Amy (Michigan State); Iriarte, Gloria (Ohio State); Jackson, Scott (Purdue); Jiang, Ning
(Michigan State); Kabelka, Eileen (U-Florida); Kamoun, Sophien (Sainsbury Labs, UK); Kozik, Alex
(UC Davis); Labate, Joanne (USDA-ARS); Lang, Lixin (Ohio State); Liu, Jia (TIGR); Loescher, Wayne
(Michigan State); Martinez, JA (IRTA, Barcelona Spain); McClure, Bruce (Univ of Missouri); Meulia,
Tea (Ohio State); Monforte, Antonio (IRTA, Barcelona Spain); Moyseenko, Jenny (Ohio State); Obando,
J (IRTA, Barcelona Spain); Olmstead, Jim (Wash State); Paran, Ilan (Volcani Center, Israel); Radovich,
Cheryll (Ohio State); Reed, Jason (U of North Carolina); Rose, Joss (Cornell); Sanyal, Abhijit (Purdue);
Schaffner, Erin (Ohio State); Sebolt, Audrey (Mich State); Song, Jing (Ohio State); Sooriyapathirana,
Suneth (Mich State); Stack, Steve (Colorado State); Stockinger, Eric (Ohio State); Stoffel, Kevin (UC
Davis); Strecker, Ben (College of Wooster); Tanksley, Steven (Cornell); Tian, Maoying (Michigan State);
van der Hoorn, Renier (Max Planck, Cologne, Germany); VanDeynze, Allen (UC-Davis); Wang, Dechun
(Michigan State); Welty, Nic (Mich State); Win, Joe (Ohio State); Yang, Wencai (Ohio State/China Agr
University).
ADVISORS
Post doctoral: Dr. Steven D. Tanksley, Cornell University
Ph.D.: Dr. Hans Kende, Michigan State University, deceased
CURRENT ADVISEES
Post doctoral researchers: Han Xiao, Gustavo Rodriguez, Zejun Huang
Ph.D.: Na Zhang, Shan Wu
MS.: Antonio Cabrera
Visiting scientist: Reynaldo Nunez
PAST ADVISEES
Post doctoral researcher: Maria Jose Gonzalo, Zaragoza, Spain
MS: Nic Welty, Michigan State
Guo-Liang Wang, PhD
Professor, Department of Plant Pathology
The Ohio State University, Columbus, OH, Phone (614) 292 9280; Fax (614) 292 4455
Email: [email protected], lab website: http://plantpath.osu.edu/~wang/
Education:
Ph.D. 1992, Plant Genetics and Breeding, University of the Philippines at Los Banos and
International Rice Research Institute (IRRI), Philippines.
M.Sc. 1985, Plant Genetics, Fujian Agricultural University, Fuzhou, China.
B.Sc. 1982, Plant Genetics, Hunan Agricultural University, Changsha, China
Research/Professional Experience:
Professor, Department of Plant Pathology, Ohio State University, Columbus, Ohio. 10/2008-present.
Associate Professor, Dept of Plant Pathology, Ohio State University, Ohio. 5/2004-9/2008.
Adjunct Professor, Hunan Agricultural University, Hunan, China, 3/2004-present.
Adjunct Professor, Institute of Genetics, Chinese Academy of Sciences, 1996present
Assistant Professor, Department of Plant Pathology, Ohio State University, Columbus, Ohio.
10/1999-5/2004.
Senior Scientist and Principal Investigator, Institute of Molecular Agrobiology, The National
University of Singapore, 8/1996-9/99.
Postdoctoral Research Associate, University of California at Davis, 8/1993-8/96
Postdoctoral Research Associate, Texas A&M University, 6/1992-8/1993.
Awards, Honors and Services
th
• Organizer, the 4 International Conference of Rice Blast, Oct. 10-14, 2007, Changsha, China.
• Editorial Board of Journal of Plant Biology, July 2007.
• Research Award of Merit from Gamma Sigma Delta, the Honor Society of Agriculture, OSU,
4/2007
• Member, Editorial Board of Plant Science, 2/2007-present
• The Syngenta Award of the American Phytopathology Society, 8/2006
• Panel member, USDA-NRI Functional Genomics, 10/2005
• OARDC Distinguished Junior Faculty Research Award, 5/2005
• FuRong Scholar, Hunan Agricultural University, Hunan, China, 3/2004-present
• Panel member: OARDC Research Committee, 9/2004-present
• Panel member: USDA-NRI Genetic Mechanism, 3/2004
• Outstanding Overseas Young Scientist Award, Natural Science Foundation of China (6/2001)
• DuPont Young Professor Award (8/2000)
Society Memberships:
American Association for the Advancement of Science (AAAS), American Society for Plant
Biologists (ASPB), American Phytopathological Society (APS)
Funding Agency: NSF-Plant Genome Research, USDA-NRI, Ohio Agricultural Research and
Development Center (OARDC) and USAID.
Research Interests: Plant Functional Genomics and Disease Resistance
Publications in the last three years (total 25)
1. Li-Rong Zeng, Chan Ho Park, R.C. Venu, Julian Gough and Guo-Liang Wang.
2008.Classification, expression pattern, and E3 ligase activity assay of rice U-Box-containing
proteins. Molecular Plant, in press.
2. Vega-Sánchez ME, Zeng L, Chen S, Leung H, Wang GL. 2008. SPIN1, a K Homology
Domain Protein Negatively Regulated and Ubiquitinated by the E3 Ubiquitin Ligase SPL11, Is
Involved in Flowering Time Control in Rice. The Plant Cell, 1456-1469.
3. Wu C, Bordeos A, Madamba MR, Baraoidan M, Ramos M, Wang GL, Leach JE, Leung H.
2008. Rice lesion mimic mutants with enhanced resistance to diseases. Mol Genet
Genomics. 279(6):605-19.
4. Shujie Dong, Lane P. Tredway, H. David Shew, Guo-Liang Wang, Elumalai Sivamani,
Rongda Qu. 2007. Resistance of transgenic tall fescue to two major fungal diseases. Plant
Science, 173:501-509.
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5. Rose Palumbo, Wai-Foong Hong, Jinguo Hu, Richard Craig, James Locke, Charles Krause,
David Tay and Guo-Liang Wang. 2007. Target Region Amplification Polymorphism (TRAP)
as a Tool for Detecting Genetic Variation in the Genus Pelargonium. HortScience 42:11181123.
6. Venu RC, Jia Y, Gowda M, Jia MH, Jantasuriyarat C, Stahlberg E, Li H, Rhineheart A,
Boddhireddy P, Singh P, Rutger N, Kudrna D, Wing R, Nelson JC, Guo-Liang Wang. 2007.
RL-SAGE and microarray analysis of the rice transcriptome after Rhizoctonia solani infection.
Mol Genet Genomics. 278: 421-431.
7. Miguel E. Vega-Sánchez, Malali Gowda and Guo-Liang Wang. 2007. Tag-based
approaches for deep transcriptome analysis in plants. Plant Sciences, 173:371-380.
8. Malali Gowda, Huameng Li, Guo-Liang Wang. 2007. Robust analysis of 5’-transcript ends
(5’-RATE): a high-throughput protocol for characterization of sequence diversity of
transcription start sites. Nature Protocols. 2(7):1622-32.
9. Malali Gowda, R-C. Venu, Huameng Li, Chatchawan Jantasuriyarat, Songbiao Chen, Maria
Bellizzi, Vishal Pampanwar, HyeRan Kim, Ralph A. Dean, Eric Stahlberg, Rod Wing, Cari
Soderlund, Guo-Liang Wang. 2007. Magnaporthe grisea Infection Triggers RNA Variation
and Antisense Transcript Expression in Rice, Plant Physiology, 144(1):524-33.
10. Kan Nobuta, Venu Reddyvari-Channarayappa, Cheng Lu, André Belo, Kalyan Vemaraju,
Pam Green, Guo-liang Wang, and Blake C. Meyers. 2007. An Expression Atlas of Rice
mRNA and Small RNA", Nature Biotechnology, 25:473-477.
11. Gowda M, RC Venu, Mohan B Raghupathy, Kan Nobuta, Huameng Li, Eric Stahlberg, Rod
Wing, Sean Coughlan, Christian D Haudenschild, Ralph Dean, Baek Hie Nahm, Blake C
Meyers and GL Wang. 2006. Deep and comparative analysis of the mycelium and
appressorium transcriptomes of Magnaporthe grisea using MPSS, RL-SAGE, and oligoarray
methods. BMC Genomics, 8;7:310.
12. Liangying Dai, Xionglun Liu, Yinghui Xiao, GL Wang. 2007 Recent advances in cloning and
characterization of disease resistance genes in rice. Journal of Integrative Plant Biology.
49 (1): 112-119.
13. Gowda M, Reddyvarichannarayappa Venu, Jia Y, Stahlberg E, Pampanwar V, Soderlund C,
GL Wang (2007). Use of RL-SAGE Analysis to Identify Novel Fungal and Plant Genes
Involved in Host-Pathogen Interactions. Methods Mol Biol. 2006;354:131-44.
Synergistic Activities:
1. Curriculum development: Agricultural Genomics PP703
2. Co-PI for the rice blast project, NSF funded 06-09
3. Co-PI for the rice MPSS transcriptome analysis, NSF funded 03-07
4. Co-PI for the rice epigenomic analysis, NSF funded 07-11
5. Member of interdisciplinary graduate training programs: Plant Molecular Biology and
Biotechnology (PMBB) at OSU
Collaborators:
Pamela Ronald, UC Davis; Hajime Sakai, DuPont; Hei Leung, The International Rice Research
Institute, Philippines; Jan Leach, Kansas State University, Ralph A. Dean, North Carolina State
University, Rod Wing, University of Arizona, Cari Soderlund, University of Arizona, Blake Meyers,
University of Delaware, Steve Jacobsen, UCLA, Liangying Dai, Hunan Agricultural University,
Advisees in Past Five Years
Guodong Lu, Professor, Fujian Agricultural and Forest University, China, Shaohong Qu, Postdoc
fellow, UC Davis, Lirong Zeng, Postdoc, Cornell University, Malali Gowda, Research Scientist, NCSU,
Bo Zhou, Associate Professor, CAS, Shanghai, China, Chat Jantasuriyarat. Assistant Professor,
Kasetsart University, Thailand
Current Advisees
Postdocs: Songbiao Chen, Honggui La, Bo Ding, Xinli Sun, IP Ahn, Maofeng Chai, Bo Zhou
Gradudate students: Miguel Vega-Sanchez, Huameng Li, Chan Ho Park, Pattavipha
Songkumarn, Gautaum Shirsekar
Visiting Scientists: Jianli Liu, Sheshu Magati, Zhongyou Pei
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