Supplemental Data
Supplemental Figure 1. Characterization of the single csn5 mutants in different growth
conditions at representative developmental stages
Supplemental Figure 1 Legend
(A) and (B) Phenotypes (A) and hypocotyls elongation (mm) (B) of five-day-old wild
type, csn5b-1, csn5a-2 and csn5a-1 mutants grown in darkness or in different light
qualities, as indicated (See Methods for details).Values shown represent the mean values
of two sets of independent experiments. The value for each experiment set was derived
from the mean of 30 seedlings (±SD). The colored boxes shown in (A) correspond to the
genotypes indicated in the legend in (B).
(C) Root elongation (mm) of wild type and csn5 mutants, grown for five days in white
light on vertical plates. Values shown represent the mean of two sets of independent
experiments. The value for each experiment set was derived from the mean of 25
seedlings (±SD).
(D) Phenotypes of eight-days-old wild type, csn5b-1 and csn5a-2 roots grown in white
light on vertical plates supplemented with 2 uM JA (See Methods for details).
(E) and (F) Morphology of inflorescences (E) (upper row pictures), flower bud clusters
(E) (bottom row pictures), flower buds (F) (left pictures) and open flowers (F) (right
pictures) of seven-week-old wild type, csn5a-2 and csn5a-1 mutant plants grown in long
day photoperiod condition. Pictures in the same row are taken at the same magnification.
(G) and (H) Morphogenetic patterns (G), relative root growth (H) (upper panel) and
number of lateral roots/4 mm of primary root (H) (lower panel) of eight-day-old wild
type, axr1-3, axr1-12, csn5b-1, csn5a-2 and csn5a-1 lines grown on vertical plates
supplemented with 2,4D. Values shown are mean of 25 seedlings (±SD). The colored
boxes shown in (G) correspond to the genotypes indicated in the legend in (H).
Characterization of the single csn5 mutants in different growth condition at
representative developmental stages (Supplemental Figure 1)
As shown in Supplemental Figure 1, when grown in the dark or under red, far-red
and blue light, both csn5a mutants have shorter hypocotyls with respect to wild type and
csn5b-1 plants (Supplemental Figure 1A and 1B). In addition, the csn5a-1 and csn5a-2
mutants display significantly shorter roots (Supplemental Figure 1C) and are impaired in
the formation of root hairs in response to jasmonic acid treatment (Zhu et al., 2006)
(Supplemental Figure 1D).
The size of the flower inflorescences and the number of flower buds in the cluster
of each inflorescence is reduced in both csn5a mutants (Supplemental Figure 1E),
suggesting a role for CSNCSN5A in the positive regulation of the inflorescence meristem
activity. Cauline leaves (data not shown), flower buds as well as mature flowers are
smaller in csn5a-1 and csn5a-2 lines than in wild type (Supplemental Figure 1F) and
csn5b-1 plants (data not shown).
Several traits of csn5a-1 and csn5a-2 phenotypes are reminiscent of the phenotype of
auxin-related mutants. It has previously been shown that csn5a-2 roots are resistant to
auxin (Dohmann et al., 2005). To confirm this result for the null csn5a-1 line, we
analyzed the root response of wild type and csn5 mutant seedlings to exogenously
applied auxin. As a control in our experiment, we used two allelic AXR1 mutants (axr1-3
and axr1-12) (Lincoln et al., 1990). Application of synthetic auxin 2,4D to wild type and
csn5b-1 results in the inhibition of primary root elongation (Supplemental Figure 1G and
1H, upper panel) and stimulation of secondary root formation (Supplemental Figure 1H,
lower panel). Conversely, csn5a-1, csn5a-2, axr1-3 and axr1-12 primary roots are
resistant to the inhibitory auxin signal and do not form secondary roots in the presence of
2,4D.
REFERENCES
Lincoln, C., Britton, J.H., and Estelle, M. (1990). Growth and development of the axr1
mutants of Arabidopsis. Plant Cell 2: 1071-1080.
Zhu, C., Gan, L., Shen, Z., and Xia, K. (2006). Interactions between jasmonate and
ethylene in the regulation of root hair development in Arabidopsis. J. Exp. Bot.
57:1299-1308.