ISOLATION OF AEROBIC BACTERIA FROM THE BUCCAL
CAVITY OF CATTLE
BY
BAMIDELE OLAWALE ABIODUN
06/1841
A
PROJECT
SUBMITTED
VETERINARY MEDICINE.IN
REQUIREMENTS
FOR
TO
THE
COLLEGE
PARTIAL FULFILMENT
THE
AWARD
OF
OF
OF THE
DOCTOR
OF
,
I
VETERINARY MEDICINE (D.V.M.) OF FEDERAL
UNIVERSITY
OF AGRICULTURE, ABEOKUTA, ABEOKUTA, NIGERIA.
JULY, 2012.
ABSTRACT
More than 700 bacteria species or phylotypes of which over 50% have not been
cultivated have been detected
in
the oral cavity. These bacteria are known as
commensals. There has been a increase in the incidence of periodontal diseases of cattle
in South western Nigeria.
,
In this study, buccal swabs were taken from 100 cattle in the Lafenwa abattoir, Ab'eokuta.
The swabs were inoculated on brain heart infusion agar plates enriched with 5% horse
blood and incubated for 24 hours at 37°C. The colonies recovered were re-innoculated on
fresh brain heart infusion agar plates until pure colonies were obtained. The bacterial
colonies obtained were identified using GNB Microbact® identification kits.
A total of 8 bacteria species were isolated and identified in this study. Of the 100 animals
evaluated in this study, Neisseria flavescens
acidophilus
was recovered from 51 %, Lactobacillus
was recovered from 54%, Staphylococcus
58%, Staphylococcus
salivarius was recovered from
•
aureus was recovered from 50%, Staphylococcus
epidermidis was
recovered from 44%, Proteus penneri was recovered from 57%, Proteus mirabilis was
recovered from 65%, Klebsiella pneumonia was recovered from 46% of the animals.
The high prevalence of these organisms in the apparently healthy cattle suggests that they
were commensals of their oral cavity.