SOPHISTICATED ANALYTICAL INSTRUMENTATION FACILITY (SAIF)
St. Peter’s Institute of Higher Education and Research
St. PETER’S UNIVERSITY
Avadi, Chennai – 600 054.
PERKIN ELMER - SPECTRUM TWO FTIR/ATR SPECTROMETER
The infrared spectrum originates from the
vibrational motion of the molecule. The vibrational
frequencies are a kind of fingerprint of the compounds. This
property is used for characterization of organic, inorganic
and biological compounds. The band intensities are
proportional to the concentration of the compound and hence
quantitative estimations are possible. The IR spectroscopy is
also carried out by using Fourier transform technique.
The Perkin Elmer Spectrum Two FT-IR instrument
consists of globar and mercury vapor lamp as sources, an
interferometer chamber comprising of KBr and mylar beam
splitters followed by a sample chamber and detector. Entire
region of 4000 – 450 cm-1 is covered by this instrument.
The spectrometer works under purged conditions. Solid
samples are dispersed in KBr or polyethylene pellets
depending on the region of interest. This instrument has a
typical resolution of 0.5 cm-1. Signal averaging, signal
enhancement, base line correction, normalization and other
spectral manipulations are possible.
The FT-IR Spectroscopy is also carried out by
using ATR Diamond Accessory. Reflection which occurs
when, an absorbing coupling mechanism acts in the process
of Total Internal Reflection to make the Reflectance less
than unity. In this process if the absorbing sample is placed
in contact with the reflecting surface, the reflectance for total
internal reflection will be attenuated to some value between
greater than zero and unity in regions of the spectrum, where
absorption of the radiant power can take place.
Description
The interference pattern obtained from a two beam
interferometer as the path difference between the two beams
is altered, when Fourier transformed, gives rise to the
spectrum. The transformation of the interferogram into
spectrum is carried out mathematically with a dedicated online computer.
Instrument Details
Model
: Spectrum Two FTIR/ATR Spectrometer
Scan Range: MIR 4000 - 450cm-1
Resolution : 0.5 cm-1
FTIR Spectrum of a Pharmaceutical Formulation
Applications
Infrared spectrum is useful in identifying the functional groups like -OH, -CN, -CO, -CH, -NH2, etc. Also quantitative
estimation is possible in certain cases for chemicals, pharmaceuticals, petroleum products, etc. Resins from industries and rubber
samples can be analyzed. Blood and food materials can also be studied. Reaction mechanisms and concentration studies are done.
PERKIN ELMER – LAMBDA 35 UV WINLAB V 6.0 AND LAMBDA EZ 201 SPECTROMETERS
The UV-VIS spectrum is due to the
electronic transitions of the molecule. This is
characteristic of a compound. Qualitative and
quantitative estimations of compounds are possible
by this non destructive technique.
Description
The Absorbance (A) of a solution at a
particular wavelength is given by Beer-Lambert's law
ect = A where c is the concentration of the
compound, t is the thickness of the cell and e is the
molar extinction coefficient characteristic of the
compound at a given wavelength. This principle is
used for quantitative measurements.
The spectrometer is well suited for samples
both in solid and the dissolved (solution) form. The
spectral range with appropriate solvent is 190-1100
nm and also it can be used to study single crystals
and powder samples. This is a double beam
instrument controlled by a microprocessor. Base line
correction, Normalization, repetitive scan, kinetics,
derivative and concentration modes are possible.
Diffuse reflectance is available for solid samples.
Instrument Details
Model
: Lambda 35 UV Winlab Spectrometer
Scan Range: 190 - 1100nm
Bandwidth : 0.5 – 4 nm (variable)
UV- Vis Spectrum of Human Blood
Applications
Qualitative and quantitative studies of materials and study of molecular structure, reaction kinetics, defect
solid state, color centres, etc. The variable bandwidth of the LAMBDA 35 offers the best solution for measurements
on solids, pastes and powders.
PERKIN ELMER – LS 45 LUMINESCENCE SPECTROMETER
Fluorescence is the emission which
results from the singlet excited state to the singlet
ground state of a molecule. The fact that
fluorescence is generally more intense and
sometimes occurs only in viscous media.
Instrument Details
Model
Optics
: LS 45 Spectrofluorimeter
: Excitation 200 – 800 nm
: Emission 200 – 650 nm
Wavelength : +1nm
Accuracy
Description
The Perkin Elmer LS 45 Luminescence
Spectrometer has been developed in conjunction
with a host of software and accessories to
address a wide range of applications that require
challenges in fluorescence, phosphorescence,
chemi or Bio- luminescence. This instrument
uses a high energy pulsed xenon source for
excitation to minimize photo bleaching of
samples and provide a long live excitation
source. The Spectrophotometer can be calibrated
by using a standard fluorescent material.
The Luminescence spectrometer offers
versatility, reliability and ease- of- use. This is an
instrument with all of the heritage, sensitivity
and reliability.
This Monochromator based instrument
uses a high energy pulsed xenon source for
excitation. The software combines the user
friendly appeal of a Windows based software
package with application specific knowledge.
The result is a powerful system that can be used
routinely for quality fluorescence analyses.
Wavelength (nm)
Fluorescent Spectrum of an Organic Compound
Applications
Because of its greater sensitivity,
fluorescence
spectroscopy
has
many
applications, it is increasingly employed for the
identification of compounds and confirmational
studies, it is a powerful technique for studying
the dynamical properties of biological molecules
in solution. Fluorescence life time measurements
can be used to obtain structural information on
the fluorescing species and on kinetics of energy
transfer.
SOPISTICATED ANALYTICAL INSTRUMENTATION FACILITY
St. Peter’s Institute of Higher Education and Research
St. PETER’S UNIVERSITY
Avadi, Chennai – 600 054.
FT–IR/ ATR Spectrometer
User Information
Date:
Name
:
Designation
:
Affiliation
:
Address for communication
:
Phone number
:
Email address
:
DD. NO. …………….. Date. …………….. Amount. …………….. Bank. ……………..
Certified that the sample submitted belong to the user mentioned above. I agree to acknowledge the usage of the
facility in all publications arising out of the SAIF, SPU, Chennai. The details of publications will be intimated to the SAIF.
Signature with date & Seal
(HOD / Principal / Guide / Managing Director)
Sample code(s)
:
Region of measurement for IR
:
-1
-1
Normal IR 4000 - 450 cm / ATR IR 4000- 450 cm
* Sampling Technique: KBr / Solution (Specify Solvent) / ATR
* Nature of the sample (solid/liquid)
:
* Whether Hygroscopic or not
:
* Do you need expanded Plots? ____________________
Result in Soft copy form
Columns marked * must be filled to ensure quick processing of samples Reports will be released only after
payment is received.
Charges for the measurement should be sent through an advance demand draft drawn in favor of “SPIHER, Avadi,
Chennai” along with the samples, to the Dean (R&D), St. Peter’s University, St. Peter’s Institute of Higher
Education and Research, Avadi, Chennai-600 054.
As per the guidelines of the St. Peter’s University (SPU), in all publications of Research work, where in the
analytical services of the SAIF have been made use of, the SPU and the SAIF shall be duly acknowledged. Kindly
send us the publication reference (Journal name /volume Number / names of the authors / date of issue of the
publication etc) to us.
SOPHISTICATED ANALYTICAL INSTRUMENTATION FACILITY (SAIF)
St. Peter’s Institute of Higher Education and Research
St. PETER’S UNIVERSITY
Avadi, Chennai – 600 054.
UV-VISIBLE SPECTROMETER
User Information
Date:
Name:
Designation:
Affiliation:
Address for Communication:
Phone Number:
E-mail Address:
DD. NO. .........................., Date. ……………, Amount……………….., Bank …………………….
Purpose for which the measurement is requested: (In brief)
Certified that the sample submitted belong to the user mentioned above. I agree to acknowledge the usage of the
facility in all publications arising out of the usage of the SAIF, SPU, Chennai. The details of publications will be intimated
to the SAIF.
Signature with date & seal
(HOD / Principal / Guide / Managing Director)
Sample Code: _________________
* Weight of Sample:
*Type of experiment:
Solution
(Tick)
Molecular Weight: _________________ (if known)
Solid
Others Specify
Molecular formula: _________________ (if known)
*Spectral region to be measured: _________ to _______ nm.
*Do you need qualitative or quantitative Measurement _________________________
*Solvent to be used in case of Solution Spectrum
Items marked * must be provided for processing this request.
_______________________________________________________________________
Charges for the measurement should be sent through an advance demand draft drawn in favor of “SPIHER, Avadi,
Chennai” along with the samples, to the Dean (R&D), St. Peter’s University, St. Peter’s Institute of Higher Education
and Research, Avadi, Chennai-600 054
.
As per the guidelines of the St. Peter’s University (SPU), in all publications of Research work, where in the analytical
services of the SAIF have been made use of, the SPU and the SAIF shall be duly acknowledged. Kindly send us the
publication reference (Journal name /volume Number / names of the authors / date of issue of the publication etc) to us.
SOPISTICATED ANALYTICAL INSTRUMENTATION FACILITY
St. Peter’s Institute of Higher Education and Research
St. PETER’S UNIVERSITY
Avadi, Chennai – 600 054.
LUMINESCENCE SPECTROMETER
User Information:
Date:
Name
:
Designation
:
Affiliation
:
Address for communication
:
Bill to be address to
:
Phone Number
:
E-mail Address
:
Purpose for which the measurement is requested:
(In brief)
DD. No. ……………….., Date. …………………..,
Amount. ……………………, Bank. ……………………
Certified that the sample submitted belong to the user mentioned above. I agree to acknowledge the usage of the facility in
all publications arising out of the SAIF, SPU, Chennai. The details of publications will be intimated to the SAIF.
Signature with date & seal
(HOD / Principal / Guide / Managing Director
SAMPLE INFORMATION
*No of samples :
*Sample Code
:
*Region of measurement:
*Excitation wavelength:
*Sample submitted:
Emission:
Excitation:
The measurements requested are in connection with
1. Academic Research leading to a degree
:
2. Sponsored Research
:
3. Research related to consultancy
:
Result in Soft copy form
Columns marked * must be filled to ensure quick processing of samples Reports will be released only after payment is received.
Charges for the measurement should be sent through an advance demand draft drawn in favor of “SPIHER, Avadi, Chennai”
along with the samples, to the Dean (R&D), St. Peter’s University, St. Peter’s Institute of Higher Education and Research, Avadi,
Chennai-600 054.
As per the guidelines of the St. Peter’s University (SPU), in all publications of Research work, where in the analytical services
of the SAIF have been made use of, the SPU and the SAIF shall be duly acknowledged. Kindly send us the publication reference
(Journal name /volume Number / names of the authors / date of issue of the publication etc) to us.
SAMPLE SUBMISSION AND MODE OF PAYMENT
How to Submit the Samples
The samples should be submitted in the prescribed format indicating the information
called for as well as your special requirement, if any. If you are sending them by post, please
enclose the payment along with the samples and address the same to:
The Dean , Research and Development
St. Peter’s University
St. Peter’s Institute of Higher Education and Research
Avadi, Chennai - 600 054.
Only on receipt of the payment along with the samples, they will be registered for
analysis and taken up for measurement as per the seniority/queue of the users of the instrument
concerned. As soon as the analysis is over, the spectra along with the receipt / bill will be sent to
the users. While submitting samples for more than one analysis separate samples are to be sent
for each.
An Important notice
As per the guidelines of the St.Peter’s University, in all publications of research work,
where in the analytical services of the SAIF have been made use of, the SPU and the SAIF,
St. Peter’s Institute of Higher Education and Research, St. Peter’s University, Chennai, shall be
duly acknowledged.
A list of charges for spectral measurements is annexed. A "Three Tier" Charge System is being
followed. Please note that:
The charges mentioned are for analysis of samples received from users within India only.
For overseas users, please contact SAIF by Email directly.
Charges are subject to change periodically
Mode of Payment
Please note the current policy on payment of analysis charges: Our current policy
requires analysis that payment be received before the samples can be registered and analysis
taken up. Please make sure that the payment is received along with the samples. Payments are to
be made only by crossed demand draft (DD).
The DD must be drawn in favour of:
St. Peter’s Institute of Higher Education and Research
payable at Chennai and must be sent along with samples and Registration form to:
The Dean , Research and Development
St. Peter’s University
St. Peter’s Institute of Higher Education and Research
Avadi, Chennai - 600 054.
Please note that in the event the DD amount/payment received is more than the actual
analyses charges incurred, it will NOT be possible to refund the excess amount paid. However,
the excess amount may be adjusted against future analyses by the same user or another user from
the same organization following a written request by Email or hard copy.
List of charges with effect from 1st JULY 2014 (Inclusive of service tax) and the
prescribed format for sample submission are available in www.stpetersuniversity.org