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simple
affordable
complete
Brant Bassam PhD
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Easy to use system
Lowest possible cost per extraction
Hands-free, walk-away operation
High speed + high throughput (96 well)
Consolidated chemistry
minimum reagents and kit types work for all sample types
Complete integrated system solution
automation, reagents, consumables, protocols, options
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Bench Top Unit
770 mm open
Work surface enclosed
by acrylic hood
390 mm closed
Dual position Manifold
1. Vacuum to Waste
2. Vacuum to Elute
Tip discard chute
4 SBS plate positions
1
2
3–8 Reagent Tubs
(variable volumes)
25 kg
3
8-Channel
pipetting head
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Under Bench
Vacuum/Waste Station
To X-tractor Gene manifold
Vacuum chamber coupling
Bottle full sensor
Pump coupling
520 mm
High power pump
Carbon exhaust filter
Waste bottle
4 L sanitary quick
disconnect bottle
PolyCap pump filter
Vacuum sensor
Software regulated
-5 to -70 kPa, 80 L/min
19 kg
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Instrument Options
UV Light
• Irradiates surfaces between runs
• Robot arm moves so light reaches all areas
• Software controlled
• Timed post-run operation
• Must be factory fitted
HEPA filter
• Positive pressure clean air supply
• Software controlled
• Integrated design
• Easy filter change
• Can be added after install
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Easy-to-use Wizard
For standard
protocols simply:
1. Click-select the
columns to extract
(in columns of 8)
2. Press Jump to End
button and start run!
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Software
Intuitive color-coded
interface mirrors actual
deck layout
Active screen prompts
(where appropriate)
For each reagent tub
position, volumes needed
are calculated for the
number of samples to be
extracted
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Barcode Reader Compatible
Sample names and information can
also be imported or exported
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Pre- and Post-Run Reports
HTML report (universal format)
Provides details of a run
Documentation & Traceability
(e.g. sample, batch and lot identifiers)
Can be directly:
Printed
Saved
E-mailed
Exported to Word
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Bind-Elute Protocol
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™
CorProtocols for different applications
Available for web download
Each comprises:
PDF Protocol (Instructions for Use)
X-tractor Gene Run File
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™
CorProtocol Reagents
DNA Extraction:
P/N 2220 DX Reagent Pack
P/N 2228 DXT (Tissue Digest Reagent)
Coming Soon:
Viral Kit
(VX Reagent Pack)
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DX Reagents
Abbreviations aid recognition (DXL, DXB, etc)
DX Reagent Pack (P/N2220) comprises:
1.
2.
3.
5.
4.
6.
7.
DXL
–
–
DXB
DXW
DXF
E
DXT
Liquid Digest Reagent
DX Digest Enzyme
DX Binding Additive
Binding Reagent
Wash Reagent
Final Wash Reagent
Elution Reagent
Tissue Digest Reagent
(Reagent is for separate purchase if required)
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P/N2221
P/N2222
P/N2224
P/N2223
P/N2225
P/N2226
P/N2227
P/N2228
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X-tractor
Consumable Pack (standard)
P/N 2270 Sufficient for 480 Extractions
960 x OnCor™ C 200 µL Tips
5 x Capture Plates
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5 x Square-well Lysis Blocks
5 x Elution Plates
(96 plain tube cluster rack)
60 x Elution Plate 8-Strip Caps
5 x Sealing Films
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Lysis Plate
Square well design for maximum capacity (2.2 mL/well)
P/N 2271 96 Square-well Lysis Plate
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Capture Plate
Standard Plate
~10 µg capacity
P/N 0913 Capture Plate, Low Skirt, 800µL
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Elution Plate
Custom designed rack for X-tractor Gene
Convenient format
Grid-referenced lid design
Tab-lock on lid
Cluster rack design
(separate tubes)
P/N 2147 Elution Plates, 96 Plain Tube Cluster Rack
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Seals
P/N 1636 Elution Plate 8-Strip Caps
Silicon press-on type
Grid referenced (A–H)
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P/N 2280 Sealing Film
Seals and protects any unused
portion of a capture plate
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™
OnCor C Tips
rack design
SBS rack with replaceable lid for easy
removal and storage between runs
Total rack enclosure for security and
contamination resistance
Fully pocketed rack design
Separate pockets for each tip prevents crosscontamination, allowing tip re-use during a run
• Tip re-use reduces costs
• Uses only 1.5 tip racks per 96 extractions
P/N 2299 OnCor™ C 200 µL Fine Bore Tip
96 tips/rack, 10 racks/box
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™
OnCor C Tips
design evolution
Unmodified tip
High-Set Filter Design
OnCor™ C Design
• Filter set too low
• Better liquid clearance height
• Best liquid clearance height
• Easily wet and blocked
• Less wetting issues
• Optimized barrel and taper
• Improved filter matrix
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™
OnCor C Tips
Advanced robotic tip design
exclusive to Corbett Robotics
High-Set filter guards
against filter wetting
Capp™ compatible
hub dimensions
(“C” tips)
Larger filter and new
more effective matrix
Optimized terminal taper
minimizes sample immersion
and displacement effects
Fine bore tip for precision
liquid handling
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Wide upper barrel for
greater liquid capacity and
clearance between the liquid
and filter inside the tip
Low retention polymer
for improved liquid shedding
and pipetting precision
P/N 2299 OnCor™ C 200 µL Fine Bore Tip
96 tips/rack, 10 racks/box
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™
OnCor C Tips
Unmodified tip
improved liquid clearance height
High-Set Filter
OnCor™ C
Example
wet filter
clearance height
with 200 µL liquid
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Disposable Reagent Tubs
Convenient
Streamlines setup
Graduated for volume (mL)
Reusable lids curb evaporation
Extremely low reagent dead volume
(e.g. 0.75 mL in a 70 mL tub)
P/N 2137 70 mL size
P/N 2136 170 mL size
P/N 2314 270 mL size
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™
CorProtocol
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Options
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Options
High Yield Capture Plate
~40 µg capacity
P/N 1863 High Yield Capture Plate
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Tube Options
Cap options:
Grid referenced tubes in racks with
individual Twist-Lock caps*
Volumes:
Silicon 8-strip caps
0.65 mL Standard tubes
0.5 mL Twist-Lock tubes
P/N 1636
Label options:
1.
Grid Reference*
2.
2D Cipher Barcode*
3.
Plain Unlabelled
(P/N 2147 in purple rack, not shown)
H9
*from ABgene or authorized distributor only
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3rd Party Chemistry Options
Accessories are available to use a wide range of kits
Please note Corbett Robotics cannot provide support for reagents and plasticware of other manufacturers
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Sample Digest Option
Vortemp 56 Shaking Incubator
Can improve speed & completeness of digest
Variable vortex speed—200 to 1500 rpm
Broad temperature range to 100°C
Interchangeable platforms for
Microtubes and Microplates
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DX Reagent Verification Testing
Experimental setup
1
2
3
4
5
6
7
8
9
10
11 12
A
B
Reagents and Consumables
DX Reagent Pack (P/N2220) was
functionally tested using Whole Blood
CorProtocol™ standard X-tractor
Consumable Pack (standard; P/N 2270).
C
D
E
F
G
H
Samples
Whole bovine blood was collected in an EDTA Vaccutainer
Maximum whole blood sample size was tested (200 µL)
96 samples total were processed (whole 96-well plate);
48 whole blood and 48 no template controls (NTCs)
To test for well-to-well cross contamination, samples were extracted in a
checkerboard pattern (as shown) alternating positive samples (●) and NTCs (●).
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DX Reagent Verification Testing cont.
Gel electrophoresis of raw extracted DNA from positive samples:
reproducible yields of high quality, intact high molecular weight DNA (~40 kb)
MW B1 D1
F1 H1
A2 C2 E2 G2
MW B7 D7
F7 H7
A8 C8 E8 G8 B9 D9 F9 H9 A10 C10 E10 G10 B11 D11 F11 H11 A12 C12 E12 G12 MW
B3 D3 F3 H3 A4 C4 E4
G4 B5 D5 F5 H5 A6 C6 E6 G6 MW
40 Kb
20 Kb
10 Kb
6 Kb
4 Kb
3 Kb
2 Kb
1 Kb
40 Kb
20 Kb
10 Kb
6 Kb
4 Kb
3 Kb
2 Kb
1 Kb
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DX Reagent Verification Testing cont.
Spectrophotometer analysis of all positive samples
DNA Concentration ng/µL
2.000
90
1.800
80
1.600
70
1.400
60
ng/µL DNA
260/280 Ratio
260/280 Ratio
1.200
1.000
0.800
(from 180 µL whole blood eluted in 150 µL)
50
40
30
0.600
20
0.400
0.200
10
0.000
0
0
8
Sample
16
24
32
Sample #
40
0
48
8
16
24
32
40
Sample #
dsDNA Yield
Vol. Yield
Concentration
A260/280 Ratio
Maximum
9.39 µg
125 µL
77.0 ng/ µL
1.89
Minimum
7.26 µg
119 µL
60.0 ng/ µL
1.84
Average
8.41 µg
121.7 µL
69.1 ng/ µL
1.87
Std. Dev.
0.52 µg
1.59 µL
4.4 ng/ µL
0.01
Note: the results shown are indicative only, actual yields will vary depending on the species and the
health of the individual from which blood was harvested.
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DX Reagent Verification Testing cont.
Real-time amplification analysis of all samples
0.25
0.20
Real-Time Analysis
Real-Time quantitative amplification analysis was
done on all samples targeting the G6PD1 gene to
single copy sensitivity (40 cycles) to test for
inhibitors and for evidence of cross-contamination
DX Reagent Verification Run
■ Positive samples
■ NTC samples
0.15
0.10
Maximum CT
Minimum CT
Average CT
CT Std. Dev.
25.04
22.19
24.59
0.18
Amplification Results
• early, reproducible amp. of all positive samples
• no evidence of PCR inhibitors
Cross-contamination Results
• no amplification of alternate NTC samples
• no evidence of cross-contamination
0.05
Threshold
0.00
5
10
15
20
Cycle
25
30
35
40
Using the X-tractor Gene™ will minimise any lab’s contamination risk!
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Some sample types successfully extracted
Blood
whole, fresh, frozen, buffy coat
Human, Mouse, Turtle, Bovine…
Serum/Plasma, HIV, Hep B , Hep C, CMV
Urine
Chlamydia and N. gonorrhoea
Swabs
HSV1 and 2
Tissue
Mouse Tails
Prawn Tissue
Bird Quill Pulp
Nasal Aspirates
MPV (a single stranded RNA Virus)
Cultured cells
Micro Organisms Bacteria (Gram Pos. and Gram Neg.) and Fungi
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Benefits
Consistency & Workflow
Reduce human error and free-up technician time
Standardisation
Between labs and runs
Documentation
Pre- and Post-Run reports
Safety
No phenol/chloroform
All waste safely contained for disposal
Harmful fumes are carbon filtered out
Work area completely enclosed by hood
Run pauses if the hood is opened
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Summary
Complete-Solution
Instrument, reagents, consumables, protocols
Substantial Cost Reduction
About half other methods
Minimum Possible Cross-Contamination Risk
Optimized liquid handling strategies
High-Throughput
96 samples in about 1.5 hours
Versatility and Choice
Compatible with a wide range of protocols and 3rd party chemistries
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96-well Workflow Integration
Gene-Disc™ 100
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Offices
Brisbane Australia
Corbett Robotics Pty Ltd
42 McKechnie Drive
Eight Mile Plains, QLD 4113
T +61 7 3841 7077
F +61 7 3841 6077
Sydney Australia
Corbett Research Pty Ltd
14 Hilly Street
Mortlake, NSW 2137
T +61 2 9736 1320
F +61 2 9736 1364
E-mail [email protected]
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United Kingdom
Corbett Research UK Ltd
Unit 1 Terek House, Sandpiper Court
Phoenix Business Park, Eaton Socon
St Neots, Cambridgeshire, PE19 8EP
T +44 (0) 1480 407222
F +44 (0) 1480 407999
USA
Corbett Robotics Inc
185 Berry Street, Suite 5200
San Francisco, CA 94107 USA
T +1 415 348 1166
F +1 415 348 1177
Web www.corbettlifescience.com
All slides  2006 Corbett Life Science. All rights reserved