Table S1 List of MS-identified O-GlcNAc metabolically labeled proteins and their assigned peptide counts
derived from proteomics with probe Biotin-Diazo-Alkyne.
O-GlcNAc metabolically labeled cell lysates were clicked to Biotin-Diazo-Alkyne. After enrichment on streptavidin
beads, the bound proteins were eluted by boiling for 5min. Eluted proteins were separated on SDS-PAGE. The
protein bands were then sliced from the gel and subjected to in-gel trypsin digestion for MS analysis. Proteins that
were identified in both probe Biotin-Diazo-Alkyne and dbOGAP database were in red font. Proteins that were
identified in both probe Biotin-Diazo-Alkyne and probe Biotin-DIBO-Alkyne were highlighted in yellow color.
#
Protein names
1
Putative annexin A2-like
protein
Eukaryotic translation
initiation factor 3 subunit Clike protein
Unconventional myosin-Ic
Putative P2Y purinoceptor
10
Importin-5
Procollagen-lysine,2oxoglutarate 5-dioxygenase 2
ATP-dependent RNA
helicase DDX3X
Zinc finger protein 197
Exportin-1
Protein phosphatase 1G
Heterogeneous nuclear
ribonucleoprotein R
Alpha-actinin-4
Synaptogyrin-2
Protein O-GlcNAcase (OGA)
UDP-glucose 6dehydrogenase
Bromodomain-containing
protein 4
Filamin-B
U5 small nuclear
ribonucleoprotein 200 kDa
helicase
Isocitrate dehydrogenase
Mitochondrial import
receptor subunit TOM70
Keratin, type II cytoskeletal
75
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
Accession Number
coverage
(%)
2.95
# of
spectrums
3
# of
peptides
1
sp|B5ME19|EIFCL_HUMAN
1.09
1
1
sp|O00159|MYO1C_HUMAN
sp|O00398|P2Y10_HUMAN
0.85
14.75
1
1
1
1
sp|O00410|IPO5_HUMAN
sp|O00469|PLOD2_HUMAN
1.91
1.63
2
1
2
1
sp|O00571|DDX3X_HUMAN
1.06
1
1
sp|O14709|ZN197_HUMAN
sp|O14980|XPO1_HUMAN
sp|O15355|PPM1G_HUMAN
sp|O43390|HNRPR_HUMAN
0.68
0.75
2.01
1.9
1
1
1
1
1
1
1
1
sp|O43707|ACTN4_HUMAN
sp|O43760|SNG2_HUMAN
sp|O60502|OGA_HUMAN
sp|O60701|UGDH_HUMAN
8.01
3.57
1.97
11.94
7
1
2
8
6
1
2
5
sp|O60885|BRD4_HUMAN
0.66
1
1
sp|O75369|FLNB_HUMAN
sp|O75643|U520_HUMAN
4.5
0.47
11
1
10
1
sp|O75874|IDHC_HUMAN
sp|O94826|TOM70_HUMAN
5.8
1.81
2
1
2
1
sp|O95678|K2C75_HUMAN
6.9
8
4
sp|A6NMY6|AXA2L_HUMAN
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
L-lactate dehydrogenase A
chain (LDH-A)
Retinal dehydrogenase 1
Glutamate dehydrogenase 1
Phosphoglycerate kinase 1
Polymeric immunoglobulin
receptor
Ig alpha-1 chain C region
Keratin, type I cytoskeletal
14
Keratin, type II cytoskeletal
6A
Prelamin-A/C
Serum albumin
Lactotransferrin
Keratin, type II cytoskeletal
6B
Keratin, type II cytoskeletal
1
Sodium/potassiumtransporting ATPase subunit
alpha-1
Aldehyde dehydrogenase
Protein S100-A8
Integrin beta-1
Keratin, type I cytoskeletal
18
Keratin, type II cytoskeletal
8
Alpha-enolase
Glucose-6-phosphate
isomerase
Epoxide hydrolase 1
Protein disulfide-isomerase
Tubulin beta chain
Bifunctional
glutamate/proline--tRNA
ligase
Heat shock protein HSP 90alpha
4F2 cell-surface antigen
heavy chain
Heat shock protein HSP 90beta
Vimentin
Keratin, type II cytoskeletal
7
Keratin, type I cytoskeletal
16
40S ribosomal protein SA
sp|P00338|LDHA_HUMAN
3.01
1
1
sp|P00352|AL1A1_HUMAN
sp|P00367|DHE3_HUMAN
sp|P00558|PGK1_HUMAN
sp|P01833|PIGR_HUMAN
28.34
2.15
3.84
1.05
28
1
2
1
13
1
2
1
sp|P01876|IGHA1_HUMAN
sp|P02533|K1C14_HUMAN
17
29.45
9
55
6
14
sp|P02538|K2C6A_HUMAN
28.37
48
16
sp|P02545|LMNA_HUMAN
sp|P02768|ALBU_HUMAN
sp|P02788|TRFL_HUMAN
sp|P04259|K2C6B_HUMAN
1.36
5.09
1.41
26.77
1
21
1
48
1
4
1
15
sp|P04264|K2C1_HUMAN
46.27
166
28
sp|P05023|AT1A1_HUMAN
3.23
3
3
sp|P05091|ALDH2_HUMAN
sp|P05109|S10A8_HUMAN
sp|P05556|ITB1_HUMAN
sp|P05783|K1C18_HUMAN
3.87
11.83
3.88
5.12
3
1
4
9
2
1
3
3
sp|P05787|K2C8_HUMAN
6
14
4
sp|P06733|ENOA_HUMAN
sp|P06744|G6PI_HUMAN
12.67
10.39
4
7
4
6
sp|P07099|HYEP_HUMAN
sp|P07237|PDIA1_HUMAN
sp|P07437|TBB5_HUMAN
sp|P07814|SYEP_HUMAN
3.3
16.34
18.92
2.71
2
8
25
3
2
8
8
3
sp|P07900|HS90A_HUMAN
19.81
26
13
sp|P08195|4F2_HUMAN
3.65
3
2
sp|P08238|HS90B_HUMAN
18.78
24
12
sp|P08670|VIME_HUMAN
sp|P08729|K2C7_HUMAN
13.3
20.68
6
10
6
9
sp|P08779|K1C16_HUMAN
38.69
60
17
sp|P08865|RSSA_HUMAN
7.12
2
2
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
Dihydrolipoyl
dehydrogenase
Poly [ADP-ribose]
polymerase 1
Leucine-rich repeat and
immunoglobulin-like
domain-containing nogo
receptor-interacting protein 3
Polyubiquitin-B
Heat shock 70 kDa protein
1A
60 kDa heat shock protein
78 kDa glucose-regulated
protein
Heat shock cognate 71 kDa
protein
Glycogen phosphorylase,
brain form
Glucose-6-phosphate 1dehydrogenase
C-1-tetrahydrofolate
synthase
Keratin, type II cytoskeletal
3
Alpha-actinin-1
Xaa-Pro dipeptidase
X-ray repair crosscomplementing protein 5
Ribonuclease inhibitor
Elongation factor 2
Keratin, type I cytoskeletal
10
Keratin, type I cytoskeletal
13
Keratin, type II cytoskeletal
5
Protein disulfide-isomerase
A4
Prolyl 4-hydroxylase subunit
alpha-1
Glucosidase 2 subunit beta
Pyruvate kinase PKM
Endoplasmin
Heterogeneous nuclear
ribonucleoprotein L
Junction plakoglobin
Desmoplakin
NADPH--cytochrome P450
reductase
Sarcoplasmic/endoplasmic
reticulum calcium ATPase 2
sp|P09622|DLDH_HUMAN
3.73
2
2
sp|P09874|PARP1_HUMAN
0.99
1
1
sp|P0C6S8|LIGO3_HUMAN
1.01
1
1
sp|P0CG47|UBB_HUMAN
sp|P0DMV8|HS71A_HUMAN
11.79
12.01
1
9
1
6
sp|P10809|CH60_HUMAN
sp|P11021|GRP78_HUMAN
11.52
14.07
7
7
7
7
sp|P11142|HSP7C_HUMAN
18.27
16
11
sp|P11216|PYGB_HUMAN
1.07
1
1
sp|P11413|G6PD_HUMAN
20.78
13
10
sp|P11586|C1TC_HUMAN
1.71
1
1
sp|P12035|K2C3_HUMAN
3.34
8
3
sp|P12814|ACTN1_HUMAN
sp|P12955|PEPD_HUMAN
sp|P13010|XRCC5_HUMAN
4.71
1.62
1.37
6
1
1
4
1
1
sp|P13489|RINI_HUMAN
sp|P13639|EF2_HUMAN
sp|P13645|K1C10_HUMAN
8.68
7.11
38.7
3
7
114
3
6
25
sp|P13646|K1C13_HUMAN
10.26
23
6
sp|P13647|K2C5_HUMAN
25.59
45
16
sp|P13667|PDIA4_HUMAN
5.58
4
3
sp|P13674|P4HA1_HUMAN
2.62
1
1
sp|P14314|GLU2B_HUMAN
sp|P14618|KPYM_HUMAN
sp|P14625|ENPL_HUMAN
sp|P14866|HNRPL_HUMAN
2.46
24.86
12.58
6.28
1
17
14
3
1
11
9
2
sp|P14923|PLAK_HUMAN
sp|P15924|DESP_HUMAN
sp|P16435|NCPR_HUMAN
2.55
0.94
1.62
3
3
1
2
3
1
sp|P16615|AT2A2_HUMAN
0.96
2
1
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
Alpha-Nacetylgalactosaminidase
Heat shock 70 kDa protein 6
Calpain-2 catalytic subunit
Nucleolin
Eukaryotic translation
initiation factor 2 subunit 2
Annexin A7
Filamin-A
Protein-glutamine gammaglutamyltransferase 2
Multifunctional protein
ADE2
Ubiquitin-like modifieractivating enzyme 1
Adenosylhomocysteinase
DNA-directed RNA
polymerase II subunit RPB1
ATP synthase subunit alpha
Moesin
Elongation factor 1-gamma
Calreticulin
Microtubule-associated
protein 4
Calnexin
Sperm-specific antigen 2
Transketolase
Protein disulfide-isomerase
A3
Serine/threonine-protein
phosphatase 2A 65 kDa
regulatory subunit A alpha
isoform
Aldehyde dehydrogenase,
dimeric NADP-preferring
Carbamoyl-phosphate
synthase
Cytochrome b-c1 complex
subunit 1
Heterogeneous nuclear
ribonucleoprotein H
Stress-inducedphosphoprotein 1
Heat shock 70 kDa protein 4
Keratin, type I cytoskeletal 9
Myosin-9
Myosin-10
Keratin, type II cytoskeletal
2 epidermal
sp|P17050|NAGAB_HUMAN
11.68
1
1
sp|P17066|HSP76_HUMAN
sp|P17655|CAN2_HUMAN
sp|P19338|NUCL_HUMAN
sp|P20042|IF2B_HUMAN
5.91
1.43
3.66
3
5
1
3
1
3
1
3
1
sp|P20073|ANXA7_HUMAN
sp|P21333|FLNA_HUMAN
sp|P21980|TGM2_HUMAN
1.84
8.95
6.99
1
18
4
1
18
4
sp|P22234|PUR6_HUMAN
4.47
2
2
sp|P22314|UBA1_HUMAN
1.23
1
1
sp|P23526|SAHH_HUMAN
sp|P24928|RPB1_HUMAN
7.64
0.46
3
1
3
1
sp|P25705|ATPA_HUMAN
sp|P26038|MOES_HUMAN
sp|P26641|EF1G_HUMAN
sp|P27797|CALR_HUMAN
sp|P27816|MAP4_HUMAN
6.33
6.76
2.29
7.43
4.95
3
4
1
3
4
3
4
1
2
4
sp|P27824|CALX_HUMAN
sp|P28290|SSFA2_HUMAN
sp|P29401|TKT_HUMAN
sp|P30101|PDIA3_HUMAN
5.07
0.87
9.31
8.71
3
1
4
4
3
1
4
4
sp|P30153|2AAA_HUMAN
3.57
2
2
sp|P30838|AL3A1_HUMAN
18.1
10
7
sp|P31327|CPSM_HUMAN
1.13
2
2
sp|P31930|QCR1_HUMAN
2.08
1
1
sp|P31943|HNRH1_HUMAN
2.67
2
2
7
4
4
1.31
33.55
12.76
1.21
30.99
1
110
26
2
81
1
17
22
2
18
sp|P31948|STIP1_HUMAN
sp|P34932|HSP74_HUMAN
sp|P35527|K1C9_HUMAN
sp|P35579|MYH9_HUMAN
sp|P35580|MYH10_HUMAN
sp|P35908|K22E_HUMAN
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
26S protease regulatory
subunit 7
Sterol regulatory elementbinding protein 1
Stress-70 protein
Eukaryotic initiation factor
4A-III
Eukaryotic translation
initiation factor 2 subunit 3
Leucine-rich PPR motifcontaining protein
Matrin-3
Aldehyde dehydrogenase
family 3 member B1
Nicotinamide
phosphoribosyltransferase
Ubiquitin carboxyl-terminal
hydrolase 5
Antigen KI-67
Microtubule-associated
protein 1B
Utrophin
Ras GTPase-activating-like
protein IQGAP1
T-complex protein 1 subunit
epsilon
Keratin, type II cytoskeletal
6C
4trimethylaminobutyraldehyde
dehydrogenase
Nuclear autoantigenic sperm
protein
Fatty acid synthase
T-complex protein 1 subunit
gamma
Elongation factor Tu
Alanine--tRNA ligase
Hsc70-interacting protein
T-complex protein 1 subunit
theta
T-complex protein 1 subunit
delta
6-phosphogluconate
dehydrogenase,
decarboxylating
Heterogeneous nuclear
ribonucleoprotein M
ATP-citrate synthase
Arginine--tRNA ligase
Ubiquitin carboxyl-terminal
hydrolase 14
sp|P35998|PRS7_HUMAN
3
1
1
sp|P36956|SRBP1_HUMAN
0.78
1
1
sp|P38646|GRP75_HUMAN
sp|P38919|IF4A3_HUMAN
10.16
1.95
6
1
6
1
sp|P41091|IF2G_HUMAN
2.12
1
1
sp|P42704|LPPRC_HUMAN
1.43
2
2
sp|P43243|MATR3_HUMAN
sp|P43353|AL3B1_HUMAN
4.01
1.92
3
1
3
1
sp|P43490|NAMPT_HUMAN
8.35
4
4
sp|P45974|UBP5_HUMAN
1.75
1
1
sp|P46013|KI67_HUMAN
sp|P46821|MAP1B_HUMAN
2.83
8.02
7
16
7
15
sp|P46939|UTRO_HUMAN
sp|P46940|IQGA1_HUMAN
1.34
1.57
1
2
1
2
sp|P48643|TCPE_HUMAN
4.99
3
3
sp|P48668|K2C6C_HUMAN
28.37
49
16
sp|P49189|AL9A1_HUMAN
2.43
1
1
sp|P49321|NASP_HUMAN
4.95
4
4
sp|P49327|FAS_HUMAN
sp|P49368|TCPG_HUMAN
2.11
8.07
6
5
5
4
sp|P49411|EFTU_HUMAN
sp|P49588|SYAC_HUMAN
sp|P50502|F10A1_HUMAN
sp|P50990|TCPQ_HUMAN
8.19
1.24
3.79
12.96
3
1
1
7
3
1
1
7
sp|P50991|TCPD_HUMAN
1.48
1
1
sp|P52209|6PGD_HUMAN
12.01
6
5
sp|P52272|HNRPM_HUMAN
3.56
2
2
sp|P53396|ACLY_HUMAN
sp|P54136|SYRC_HUMAN
sp|P54578|UBP14_HUMAN
3.18
1.21
2.63
3
1
1
3
1
1
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
Solute carrier family 12
member 2
Transitional endoplasmic
reticulum ATPase
Trifunctional enzyme subunit
beta
Nucleosome assembly
protein 1-like 1
Eukaryotic translation
initiation factor 3 subunit B
Methionine--tRNA ligase
Eukaryotic translation
initiation factor 3 subunit E
Actin, cytoplasmic 1 (Betaactin)
Ubiquitin-conjugating
enzyme E2 D3
Actin-related protein 3
Heterogeneous nuclear
ribonucleoprotein K
26S protease regulatory
subunit 4
Actin, alpha cardiac muscle 1
Elongation factor 1-alpha 1
Tubulin alpha-1B chain
Tubulin beta-4B chain
Keratin, type II cuticular Hb5
DNA-dependent protein
kinase catalytic subunit
Dermcidin
Phosphate carrier protein
Clathrin heavy chain 1
Heterogeneous nuclear
ribonucleoprotein U
Spectrin beta chain, nonerythrocytic 1
ATP-dependent 6phosphofructokinase, platelet
type
Peptidyl-prolyl cis-trans
isomerase FKBP4
A-kinase anchor protein 12
Eukaryotic translation
initiation factor 4 gamma 1
Keratin, type I cytoskeletal
17
Cytoskeleton-associated
protein 4
ATP-dependent RNA
helicase A
Histone-binding protein
RBBP4
sp|P55011|S12A2_HUMAN
0.83
1
1
sp|P55072|TERA_HUMAN
4.96
4
4
sp|P55084|ECHB_HUMAN
1.9
1
1
sp|P55209|NP1L1_HUMAN
6.91
2
2
sp|P55884|EIF3B_HUMAN
1.11
1
1
sp|P56192|SYMC_HUMAN
sp|P60228|EIF3E_HUMAN
1.11
2.47
1
1
1
1
sp|P60709|ACTB_HUMAN
32
23
11
sp|P61077|UB2D3_HUMAN
7.48
1
1
sp|P61158|ARP3_HUMAN
sp|P61978|HNRPK_HUMAN
2.15
9.94
1
4
1
4
sp|P62191|PRS4_HUMAN
2.73
1
1
sp|P68032|ACTC_HUMAN
sp|P68104|EF1A1_HUMAN
sp|P68363|TBA1B_HUMAN
sp|P68371|TBB4B_HUMAN
sp|P78386|KRT85_HUMAN
sp|P78527|PRKDC_HUMAN
25.2
5.84
21.06
18.88
8.68
0.99
16
4
11
25
6
5
9
3
7
8
5
4
sp|P81605|DCD_HUMAN
sp|Q00325|MPCP_HUMAN
sp|Q00610|CLH1_HUMAN
sp|Q00839|HNRPU_HUMAN
20
3.31
2.93
1.82
4
1
6
2
2
1
5
2
sp|Q01082|SPTB2_HUMAN
3.47
8
8
sp|Q01813|PFKAP_HUMAN
2.3
2
2
sp|Q02790|FKBP4_HUMAN
8.71
4
4
sp|Q02952|AKA12_HUMAN
sp|Q04637|IF4G1_HUMAN
2.53
1.06
4
1
4
1
sp|Q04695|K1C17_HUMAN
17.36
32
9
sp|Q07065|CKAP4_HUMAN
8.47
4
4
sp|Q08211|DHX9_HUMAN
2.91
4
4
sp|Q09028|RBBP4_HUMAN
3.06
2
1
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
Tumor suppressor p53binding protein 1
Heat shock protein 75 kDa
26S proteasome non-ATPase
regulatory subunit 2
Sequestosome-1
Tubulin beta-3 chain
Spectrin alpha chain, nonerythrocytic 1
Heterogeneous nuclear
ribonucleoprotein D0
Eukaryotic translation
initiation factor 3 subunit A
Neutral alpha-glucosidase
AB
Golgin subfamily B member
1
Importin subunit beta-1
Nuclear mitotic apparatus
protein 1
Protein disulfide-isomerase
A6
Plectin
Pericentriolar material 1
protein
Keratin, type I cuticular Ha1
Calcium/calmodulindependent protein kinase
type IV
Drebrin
Thioredoxin reductase 1
Putative hexokinase HKDC1
Leucine-rich repeat
flightless-interacting protein
1
Filaggrin-2
Brefeldin A-inhibited
guanine nucleotide-exchange
protein 3
Rootletin
BRO1 domain-containing
protein BROX
Keratin, type II cytoskeletal
79
Keratin, type II cytoskeletal
80
Pre-mRNA-processingsplicing factor 8
Coiled-coil domaincontaining protein 81
Transcription elongation
factor SPT6
sp|Q12888|TP53B_HUMAN
1.22
2
2
sp|Q12931|TRAP1_HUMAN
sp|Q13200|PSMD2_HUMAN
1.99
1.32
1
1
1
1
sp|Q13501|SQSTM_HUMAN
sp|Q13509|TBB3_HUMAN
sp|Q13813|SPTN1_HUMAN
10.45
12.44
3.64
3
14
9
2
5
9
sp|Q14103|HNRPD_HUMAN
6.76
2
2
sp|Q14152|EIF3A_HUMAN
1.88
3
3
sp|Q14697|GANAB_HUMAN
0.85
1
1
sp|Q14789|GOGB1_HUMAN
0.25
2
1
sp|Q14974|IMB1_HUMAN
sp|Q14980|NUMA1_HUMAN
1.94
0.57
2
1
2
1
sp|Q15084|PDIA6_HUMAN
6.59
2
2
sp|Q15149|PLEC_HUMAN
sp|Q15154|PCM1_HUMAN
5.23
0.44
22
1
22
1
sp|Q15323|K1H1_HUMAN
sp|Q16566|KCC4_HUMAN
6.49
1.69
9
1
3
1
sp|Q16643|DREB_HUMAN
sp|Q16881|TRXR1_HUMAN
sp|Q2TB90|HKDC1_HUMAN
sp|Q32MZ4|LRRF1_HUMAN
4.01
3.08
2.07
2.85
2
2
2
2
2
2
2
2
sp|Q5D862|FILA2_HUMAN
sp|Q5TH69|BIG3_HUMAN
0.5
2.25
3
1
1
1
sp|Q5TZA2|CROCC_HUMAN
sp|Q5VW32|BROX_HUMAN
0.4
2.68
1
1
1
1
sp|Q5XKE5|K2C79_HUMAN
5.23
6
3
sp|Q6KB66|K2C80_HUMAN
1.99
2
1
sp|Q6P2Q9|PRP8_HUMAN
0.43
1
1
sp|Q6ZN84|CCD81_HUMAN
1.53
1
1
sp|Q7KZ85|SPT6H_HUMAN
0.7
1
1
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
Staphylococcal nuclease
domain-containing protein 1
Myosin-14
Coiled-coil domaincontaining protein C1orf110
Hornerin
Protein unc-80 homolog
GH3 domain-containing
protein
CMT1A duplicated region
transcript 4 protein
Thioredoxin domaincontaining protein 5
Protein NDRG1
Translational activator GCN1
Transportin-1
Signal-induced proliferationassociated protein 1
ERO1-like protein alpha
Tetratricopeptide repeat
protein 25
Vacuolar protein sortingassociated protein 35
Endoplasmic reticulum
resident protein 44
EH domain-containing
protein 4
STE20-like serine/threonineprotein kinase
Reticulon-4
Nucleolar RNA helicase 2
Phenylalanine--tRNA ligase
beta subunit
Myoferlin
Ribosome-binding protein 1
Signal recognition particle
subunit SRP68
Apoptotic chromatin
condensation inducer in the
nucleus
Serine/arginine repetitive
matrix protein 2
Proliferation-associated
protein 2G4
Epididymis-specific alphamannosidase
tRNA-splicing ligase RtcB
homolog
Talin-1
sp|Q7KZF4|SND1_HUMAN
1.21
1
1
sp|Q7Z406|MYH14_HUMAN
sp|Q86UF4|CA110_HUMAN
1.4
1.99
3
1
3
1
sp|Q86YZ3|HORN_HUMAN
sp|Q8N2C7|UNC80_HUMAN
sp|Q8N2G8|GHDC_HUMAN
2.53
0.74
1.7
3
1
1
2
1
1
sp|Q8N9R6|CDRT4_HUMAN
4.64
1
1
sp|Q8NBS9|TXND5_HUMAN
6.02
3
3
sp|Q92597|NDRG1_HUMAN
sp|Q92616|GCN1L_HUMAN
sp|Q92973|TNPO1_HUMAN
sp|Q96FS4|SIPA1_HUMAN
3.55
0.49
2.12
0.77
1
1
1
1
1
1
1
1
sp|Q96HE7|ERO1A_HUMAN
sp|Q96NG3|TTC25_HUMAN
2.99
1.64
1
1
1
1
sp|Q96QK1|VPS35_HUMAN
2.39
1
1
sp|Q9BS26|ERP44_HUMAN
2.46
1
1
sp|Q9H223|EHD4_HUMAN
3.33
2
2
sp|Q9H2G2|SLK_HUMAN
0.81
1
1
sp|Q9NQC3|RTN4_HUMAN
sp|Q9NR30|DDX21_HUMAN
sp|Q9NSD9|SYFB_HUMAN
1.09
1.4
1.87
1
1
1
1
1
1
sp|Q9NZM1|MYOF_HUMAN
sp|Q9P2E9|RRBP1_HUMAN
sp|Q9UHB9|SRP68_HUMAN
3.11
4.4
1.44
7
6
1
6
5
1
sp|Q9UKV3|ACINU_HUMAN
0.67
1
1
sp|Q9UQ35|SRRM2_HUMAN
0.51
1
1
sp|Q9UQ80|PA2G4_HUMAN
7.87
4
3
sp|Q9Y2E5|MA2B2_HUMAN
1.19
1
1
sp|Q9Y3I0|RTCB_HUMAN
2.18
1
1
sp|Q9Y490|TLN1_HUMAN
1.02
2
2
Table S2 List of MS-identified O-GlcNAc metabolically labeled proteins and their assigned peptide counts
derived from proteomics with probe Biotin-DIBO-Alkyne.
O-GlcNAc metabolically labeled cell lysates were clicked to Biotin-DIBO-Alkyne. After enrichment on streptavidin
beads, the bound proteins were eluted by boiling for 5min. Eluted proteins were separated on SDS-PAGE. The
protein bands were then sliced from the gel and subjected to in-gel trypsin digestion for MS analysis. Proteins that
were identified in both probe Biotin-DIBO-Alkyne and dbOGAP database were in green font. Proteins that were
identified in both probe Biotin-Diazo-Alkyne and probe Biotin-DIBO-Alkyne were highlighted in yellow color.
#
1
Protein names
Accession Number
coverage
(%)
2.72
# of
spectrums
1
# of
peptides
1
sp|A6NMY6|AXA2L_HUMAN
2.95
1
1
Puromycin-sensitive
aminopeptidase-like protein
Putative annexin A2-like
protein
Dynamin-1-like protein
sp|A6NEC2|PSAL_HUMAN
sp|O00429|DNM1L_HUMAN
1.22
1
1
Protein arginine Nmethyltransferase 5
Spectrin beta chain, nonerythrocytic 2
Rho guanine nucleotide
exchange factor 11
D-3-phosphoglycerate
dehydrogenase
Alpha-actinin-4
sp|O14744|ANM5_HUMAN
1.41
1
1
sp|O15020|SPTN2_HUMAN
0.25
1
1
sp|O15085|ARHGB_HUMAN
0.46
1
1
sp|O43175|SERA_HUMAN
3.56
2
2
sp|O43707|ACTN4_HUMAN
6.15
4
4
sp|O43776|SYNC_HUMAN
2.01
1
1
sp|O60568|PLOD3_HUMAN
1.36
1
1
11
Asparagine--tRNA ligase,
cytoplasmic
Procollagen-lysine,2oxoglutarate 5-dioxygenase 3
Perilipin-3
sp|O60664|PLIN3_HUMAN
3.69
1
1
12
UDP-glucose 6-dehydrogenase
sp|O60701|UGDH_HUMAN
5.26
2
2
13
Filamin-B
sp|O75369|FLNB_HUMAN
1.96
5
5
14
Citrate synthase, mitochondrial
sp|O75390|CISY_HUMAN
2.36
1
1
15
Oral-facial-digital syndrome 1
protein
Isocitrate dehydrogenase
[NADP] cytoplasmic (IDH)
ATP-dependent Clp protease
ATP-binding subunit clpX-like
E3 ubiquitin-protein ligase
UBR5
Importin-7
sp|O75665|OFD1_HUMAN
0.89
1
1
sp|O75874|IDHC_HUMAN
4.83
2
2
sp|O76031|CLPX_HUMAN
1.58
1
1
sp|O95071|UBR5_HUMAN
0.32
1
1
sp|O95373|IPO7_HUMAN
1.16
1
1
2
3
4
5
6
7
8
9
10
16
17
18
19
20
L-lactate dehydrogenase A
chain
Retinal dehydrogenase 1
sp|P00338|LDHA_HUMAN
3.01
1
1
sp|P00352|AL1A1_HUMAN
24.55
21
11
sp|P00367|DHE3_HUMAN
1.97
1
1
23
Glutamate dehydrogenase 1,
mitochondrial
Phosphoglycerate kinase 1
sp|P00558|PGK1_HUMAN
16.31
8
6
24
Keratin, type I cytoskeletal 14
sp|P02533|K1C14_HUMAN
31.14
31
15
25
Keratin, type II cytoskeletal 6A
sp|P02538|K2C6A_HUMAN
24.29
22
12
26
Prelamin-A/C
sp|P02545|LMNA_HUMAN
2.71
2
2
27
Serum albumin
sp|P02768|ALBU_HUMAN
7.06
12
5
28
Transferrin receptor protein 1
sp|P02786|TFR1_HUMAN
1.32
1
1
29
sp|P04075|ALDOA_HUMAN
3.85
1
1
30
Fructose-bisphosphate aldolase
A
Keratin, type II cytoskeletal 6B
sp|P04259|K2C6B_HUMAN
17.73
24
9
31
Keratin, type II cytoskeletal 1
sp|P04264|K2C1_HUMAN
39.75
101
23
32
sp|P04843|RPN1_HUMAN
1.98
1
1
sp|P05023|AT1A1_HUMAN
3.32
3
3
sp|P05091|ALDH2_HUMAN
18.38
8
8
sp|P05165|PCCA_HUMAN
14.56
13
9
36
Dolichyldiphosphooligosaccharide-protein glycosyltransferase
subunit 1
Sodium/potassium-transporting
ATPase subunit alpha-1
Aldehyde dehydrogenase,
mitochondrial
Propionyl-CoA carboxylase
alpha chain, mitochondrial
Integrin beta-1
sp|P05556|ITB1_HUMAN
2.51
2
2
37
Creatine kinase M-type
sp|P06732|KCRM_HUMAN
1.84
1
1
38
Alpha-enolase
sp|P06733|ENOA_HUMAN
8.06
3
3
39
Glucose-6-phosphate isomerase
(GPI)
Epoxide hydrolase 1
sp|P06744|G6PI_HUMAN
6.27
3
3
sp|P07099|HYEP_HUMAN
4.4
2
2
Protein disulfide-isomerase
(PDI)
Calpain-1 catalytic subunit
sp|P07237|PDIA1_HUMAN
3.15
1
1
sp|P07384|CAN1_HUMAN
2.38
2
2
sp|P07437|TBB5_HUMAN
15.54
14
7
sp|P07814|SYEP_HUMAN
0.46
1
1
sp|P07900|HS90A_HUMAN
21.17
22
14
sp|P08195|4F2_HUMAN
2.06
2
1
sp|P08238|HS90B_HUMAN
16.85
17
12
sp|P08559|ODPA_HUMAN
3.33
1
1
49
Tubulin beta chain (Tubulin
beta-5 chain)
Bifunctional glutamate/proline-tRNA ligase
Heat shock protein HSP 90alpha
4F2 cell-surface antigen heavy
chain
Heat shock protein HSP 90beta
Pyruvate dehydrogenase E1
component subunit alpha,
somatic form
Vimentin
sp|P08670|VIME_HUMAN
6.01
3
3
50
Keratin, type II cytoskeletal 7
sp|P08729|K2C7_HUMAN
9.17
5
4
21
22
33
34
35
40
41
42
43
44
45
46
47
48
51
Keratin, type I cytoskeletal 16
sp|P08779|K1C16_HUMAN
24.52
27
12
52
40S ribosomal protein SA
sp|P08865|RSSA_HUMAN
4.41
1
1
53
sp|P09972|ALDOC_HUMAN
6.32
1
1
54
Fructose-bisphosphate aldolase
C
Polyubiquitin-B
sp|P0CG47|UBB_HUMAN
11.79
1
1
55
Heat shock 70 kDa protein 1A
sp|P0DMV8|HS71A_HUMAN
11.7
6
6
56
sp|P10809|CH60_HUMAN
1.4
1
1
sp|P11021|GRP78_HUMAN
9.02
4
4
sp|P11142|HSP7C_HUMAN
8.67
5
4
sp|P11216|PYGB_HUMAN
3.68
3
3
sp|P11413|G6PD_HUMAN
11.84
8
6
sp|P11498|PYC_HUMAN
18.08
39
18
sp|P11586|C1TC_HUMAN
2.89
3
3
63
60 kDa heat shock protein,
mitochondrial
78 kDa glucose-regulated
protein
Heat shock cognate 71 kDa
protein
Glycogen phosphorylase, brain
form
Glucose-6-phosphate 1dehydrogenase
Pyruvate carboxylase,
mitochondrial
C-1-tetrahydrofolate synthase,
cytoplasmic
Keratin, type II cytoskeletal 3
sp|P12035|K2C3_HUMAN
3.03
5
2
64
Myosin-1
sp|P12882|MYH1_HUMAN
0.36
1
1
65
Myosin-7
sp|P12883|MYH7_HUMAN
0.62
1
1
66
sp|P13010|XRCC5_HUMAN
1.37
1
1
sp|P13473|LAMP2_HUMAN
1.95
1
1
68
X-ray repair crosscomplementing protein 5
Lysosome-associated
membrane glycoprotein 2
Ribonuclease inhibitor
sp|P13489|RINI_HUMAN
1.95
1
1
69
Elongation factor 2 (EF-2)
sp|P13639|EF2_HUMAN
6.76
8
6
70
Keratin, type I cytoskeletal 10
sp|P13645|K1C10_HUMAN
29.79
58
16
71
Keratin, type I cytoskeletal 13
sp|P13646|K1C13_HUMAN
6.33
11
4
72
Keratin, type II cytoskeletal 5
sp|P13647|K2C5_HUMAN
24.58
26
13
73
Protein disulfide-isomerase A4
sp|P13667|PDIA4_HUMAN
1.55
1
1
74
Plastin-3 (T-plastin)
sp|P13797|PLST_HUMAN
3.65
2
2
75
Glucosidase 2 subunit beta
sp|P14314|GLU2B_HUMAN
2.46
1
1
76
Pyruvate kinase PKM
sp|P14618|KPYM_HUMAN
25.8
12
11
77
Endoplasmin
sp|P14625|ENPL_HUMAN
6.72
5
5
78
sp|P14868|SYDC_HUMAN
2.59
1
1
79
Aspartate--tRNA ligase,
cytoplasmic
Heat shock 70 kDa protein 6
sp|P17066|HSP76_HUMAN
6.22
3
3
80
Calpain-2 catalytic subunit
sp|P17655|CAN2_HUMAN
5.29
3
3
81
Vinculin
sp|P18206|VINC_HUMAN
1.15
1
1
82
Keratin, type II cytoskeletal 4
sp|P19013|K2C4_HUMAN
3.56
6
2
83
Hexokinase-1
sp|P19367|HXK1_HUMAN
0.76
1
1
84
Plasma membrane calciumtransporting ATPase 1
Filamin-A
sp|P20020|AT2B1_HUMAN
0.87
1
1
sp|P21333|FLNA_HUMAN
0.68
1
1
57
58
59
60
61
62
67
85
86
sp|P21399|ACOC_HUMAN
11.7
16
10
sp|P21980|TGM2_HUMAN
4.8
4
3
sp|P22102|PUR2_HUMAN
1.88
2
2
89
Cytoplasmic aconitate
hydratase (Aconitase)
Protein-glutamine gammaglutamyltransferase 2
Trifunctional purine
biosynthetic protein adenosine3
Multifunctional protein ADE2
sp|P22234|PUR6_HUMAN
1.88
1
1
90
Ferrochelatase, mitochondrial
sp|P22830|HEMH_HUMAN
1.42
1
1
91
sp|P23526|SAHH_HUMAN
2.78
1
1
sp|P24752|THIL_HUMAN
6.32
2
2
sp|P26639|SYTC_HUMAN
1.38
1
1
94
Adenosylhomocysteinase
(AdoHcyase)
Acetyl-CoA acetyltransferase,
mitochondrial
Threonine--tRNA ligase,
cytoplasmic
Elongation factor 1-gamma
sp|P26641|EF1G_HUMAN
2.97
1
1
95
Calnexin (IP90)
sp|P27824|CALX_HUMAN
1.86
1
1
96
sp|P28331|NDUS1_HUMAN
27.1
27
17
sp|P28340|DPOD1_HUMAN
1.63
2
2
98
NADH-ubiquinone
oxidoreductase 75 kDa subunit
DNA polymerase delta
catalytic subunit
Transketolase (TK)
sp|P29401|TKT_HUMAN
6.26
3
3
99
Protein disulfide-isomerase A3
sp|P30101|PDIA3_HUMAN
1.98
1
1
100
Serine/threonine-protein
phosphatase 2A 65 kDa
regulatory subunit A alpha
isoform
Aldehyde dehydrogenase,
dimeric NADP-preferring
Rab GDP dissociation inhibitor
alpha
S-adenosylmethionine synthase
isoform type-2
Carbamoyl-phosphate synthase
sp|P30153|2AAA_HUMAN
1.87
1
1
sp|P30838|AL3A1_HUMAN
12.14
5
5
sp|P31150|GDIA_HUMAN
2.91
1
1
sp|P31153|METK2_HUMAN
3.8
1
1
3
4
4
sp|P31943|HNRH1_HUMAN
2.23
1
1
sp|P34931|HS71L_HUMAN
8.74
4
4
107
Heterogeneous nuclear
ribonucleoprotein H
Heat shock 70 kDa protein 1like
Keratin, type I cytoskeletal 9
sp|P35527|K1C9_HUMAN
24.72
42
15
108
Myosin-9
sp|P35579|MYH9_HUMAN
2.45
4
4
109
Myosin-11
sp|P35749|MYH11_HUMAN
0.41
1
1
110
Keratin, type II cytoskeletal 2
epidermal
Stress-70 protein,
mitochondrial
Flap endonuclease 1
sp|P35908|K22E_HUMAN
32.86
57
18
sp|P38646|GRP75_HUMAN
10.6
6
6
sp|P39748|FEN1_HUMAN
2.89
1
1
Trifunctional enzyme subunit
alpha
Lamina-associated polypeptide
2, isoform alpha
sp|P40939|ECHA_HUMAN
1.83
1
1
sp|P42166|LAP2A_HUMAN
1.59
1
1
87
88
92
93
97
101
102
103
104
105
106
111
112
113
114
sp|P31327|CPSM_HUMAN
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
Leucine-rich PPR motifcontaining protein,
mitochondrial
Aldehyde dehydrogenase
family 3 member B1
Nicotinamide
phosphoribosyltransferase
ATP-dependent DNA helicase
Q1
Ras GTPase-activating-like
protein IQGAP1
Glutamine--tRNA ligase
sp|P42704|LPPRC_HUMAN
4.81
6
6
sp|P43353|AL3B1_HUMAN
5.56
2
2
sp|P43490|NAMPT_HUMAN
5.91
3
3
sp|P46063|RECQ1_HUMAN
1.69
1
1
sp|P46940|IQGA1_HUMAN
0.66
1
1
sp|P47897|SYQ_HUMAN
1.16
1
1
Nuclear autoantigenic sperm
protein
Fatty acid synthase
sp|P49321|NASP_HUMAN
2.28
2
2
sp|P49327|FAS_HUMAN
3.42
9
8
T-complex protein 1 subunit
gamma
Elongation factor Tu,
mitochondrial
Alanine--tRNA ligase,
cytoplasmic
DNA primase large subunit
sp|P49368|TCPG_HUMAN
1.83
1
1
sp|P49411|EFTU_HUMAN
2.65
1
1
sp|P49588|SYAC_HUMAN
1.34
1
1
sp|P49643|PRI2_HUMAN
2.75
1
1
NADH dehydrogenase
[ubiquinone] flavoprotein 1
Rab GDP dissociation inhibitor
beta
T-complex protein 1 subunit
theta
T-complex protein 1 subunit
delta
Annexin A11
sp|P49821|NDUV1_HUMAN
2.16
1
1
sp|P50395|GDIB_HUMAN
3.37
1
1
sp|P50990|TCPQ_HUMAN
12.41
9
7
sp|P50991|TCPD_HUMAN
5.01
2
2
sp|P50995|ANX11_HUMAN
2.18
1
1
6-phosphogluconate
dehydrogenase,
decarboxylating
Heterogeneous nuclear
ribonucleoprotein M
ATP-citrate synthase
sp|P52209|6PGD_HUMAN
12.42
5
5
sp|P52272|HNRPM_HUMAN
3.56
2
2
sp|P53396|ACLY_HUMAN
0.73
1
1
Arginine--tRNA ligase,
cytoplasmic
Exportin-2
sp|P54136|SYRC_HUMAN
2.88
2
2
sp|P55060|XPO2_HUMAN
1.85
2
2
Transitional endoplasmic
reticulum ATPase
Trifunctional enzyme subunit
beta, mitochondrial (TP-beta)
Nucleosome assembly protein
1-like 1
UPF0769 protein C21orf59
sp|P55072|TERA_HUMAN
1.24
1
1
sp|P55084|ECHB_HUMAN
1.9
1
1
sp|P55209|NP1L1_HUMAN
2.56
1
1
sp|P57076|CU059_HUMAN
2.41
1
1
Actin, cytoplasmic 1 (Betaactin)
Actin-related protein 3
sp|P60709|ACTB_HUMAN
32
15
10
sp|P61158|ARP3_HUMAN
2.15
1
1
ATP-binding cassette subfamily E member 1
sp|P61221|ABCE1_HUMAN
11.69
9
6
144
Actin, alpha cardiac muscle 1
sp|P68032|ACTC_HUMAN
27.85
13
9
145
Elongation factor 1-alpha 1
sp|P68104|EF1A1_HUMAN
4.11
2
2
146
Tubulin alpha-1B chain
sp|P68363|TBA1B_HUMAN
17.96
7
6
147
Tubulin beta-4B chain
sp|P68371|TBB4B_HUMAN
18.88
15
8
148
DNA-dependent protein kinase
catalytic subunit
Clathrin heavy chain 1
sp|P78527|PRKDC_HUMAN
0.34
1
1
sp|Q00610|CLH1_HUMAN
2.15
3
3
Heterogeneous nuclear
ribonucleoprotein U
ATP-dependent 6phosphofructokinase, platelet
type
Desmoglein-1
sp|Q00839|HNRPU_HUMAN
3.27
3
2
sp|Q01813|PFKAP_HUMAN
1.28
1
1
sp|Q02413|DSG1_HUMAN
1.53
1
1
Keratin, type I cytoskeletal 17
(39.1)
Cytoskeleton-associated
protein 4
ATP-dependent RNA helicase
A (RHA)
Polypeptide Nacetylgalactosaminyltransferase
1
Acetyl-CoA carboxylase 1
(ACC1)
Spectrin alpha chain, nonerythrocytic 1
Desmoglein-2
sp|Q04695|K1C17_HUMAN
24.77
25
12
sp|Q07065|CKAP4_HUMAN
1.99
1
1
sp|Q08211|DHX9_HUMAN
2.68
3
3
sp|Q10472|GALT1_HUMAN
2.33
1
1
sp|Q13085|ACACA_HUMAN
9.63
34
23
sp|Q13813|SPTN1_HUMAN
0.44
1
1
sp|Q14126|DSG2_HUMAN
0.81
1
1
sp|Q14204|DYHC1_HUMAN
0.22
1
1
161
Cytoplasmic dynein 1 heavy
chain 1
Src substrate cortactin
sp|Q14247|SRC8_HUMAN
5.45
2
2
162
Importin subunit beta-1
sp|Q14974|IMB1_HUMAN
2.28
2
2
163
Plectin
sp|Q15149|PLEC_HUMAN
0.41
1
1
164
Pericentriolar material 1
protein
Drebrin
sp|Q15154|PCM1_HUMAN
0.49
1
1
sp|Q16643|DREB_HUMAN
2.77
1
1
sp|Q16891|MIC60_HUMAN
1.32
1
1
sp|Q1KMD3|HNRL2_HUMAN
1.87
1
1
168
MICOS complex subunit
MIC60
Heterogeneous nuclear
ribonucleoprotein U-like
protein 2
Keratin, type II cytoskeletal 79
sp|Q5XKE5|K2C79_HUMAN
5.79
10
3
169
Kinectin
sp|Q86UP2|KTN1_HUMAN
0.88
1
1
170
sp|Q86VP6|CAND1_HUMAN
1.46
2
2
171
Cullin-associated NEDD8dissociated protein 1
Phospholipase D3 (PLD 3)
sp|Q8IV08|PLD3_HUMAN
1.84
1
1
172
Spartin
sp|Q8N0X7|SPG20_HUMAN
1.8
1
1
173
Nuclear pore complex protein
Nup93
Protein FAM71E2
sp|Q8N1F7|NUP93_HUMAN
1.34
1
1
sp|Q8N5Q1|F71E2_HUMAN
0.76
1
1
149
150
151
152
153
154
155
156
157
158
159
160
165
166
167
174
175
sp|Q8NBX0|SCPDL_HUMAN
3.03
1
1
sp|Q8NFV4|ABHDB_HUMAN
3.81
1
1
sp|Q8TE73|DYH5_HUMAN
0.43
1
1
sp|Q8WUM4|PDC6I_HUMAN
1.5
1
1
179
Saccharopine dehydrogenaselike oxidoreductase
Alpha/beta hydrolase domaincontaining protein 11
Dynein heavy chain 5,
axonemal
Programmed cell death 6interacting protein
Heat shock protein 105 kDa
sp|Q92598|HS105_HUMAN
2.21
2
2
180
Translational activator GCN1
sp|Q92616|GCN1L_HUMAN
0.37
1
1
181
GPI-anchor transamidase (GPI
transamidase)
Probable ATP-dependent RNA
helicase DDX17
DNA repair protein RAD50
(hRAD50)
Golgi apparatus protein 1
sp|Q92643|GPI8_HUMAN
5.32
1
1
sp|Q92841|DDX17_HUMAN
2.61
3
2
sp|Q92878|RAD50_HUMAN
1.45
2
2
sp|Q92896|GSLG1_HUMAN
1.27
1
1
Far upstream element-binding
protein 2
PDZ and LIM domain protein 5
sp|Q92945|FUBP2_HUMAN
1.83
1
1
sp|Q96HC4|PDLI5_HUMAN
1.51
1
1
sp|Q96RQ3|MCCA_HUMAN
1.79
1
1
sp|Q99798|ACON_HUMAN
2.95
3
2
189
Methylcrotonoyl-CoA
carboxylase subunit alpha
Aconitate hydratase,
mitochondrial
Neurolysin, mitochondrial
sp|Q9BYT8|NEUL_HUMAN
1.56
1
1
190
Golgi resident protein GCP60
sp|Q9H3P7|GCP60_HUMAN
2.46
1
1
191
Aminopeptidase B (AP-B)
sp|Q9H4A4|AMPB_HUMAN
1.69
1
1
192
Elongator complex protein 3
sp|Q9H9T3|ELP3_HUMAN
3.29
2
2
193
Myoferlin
sp|Q9NZM1|MYOF_HUMAN
0.53
1
1
194
Ataxin-10
sp|Q9UBB4|ATX10_HUMAN
3.16
1
1
195
Testin (TESS)
sp|Q9UGI8|TES_HUMAN
2.85
1
1
196
Exportin-7
sp|Q9UIA9|XPO7_HUMAN
0.83
1
1
197
sp|Q9Y2K1|ZBTB1_HUMAN
1.96
1
1
sp|Q9Y3I0|RTCB_HUMAN
2.18
1
1
199
Zinc finger and BTB domaincontaining protein 1
tRNA-splicing ligase RtcB
homolog
Talin-1
sp|Q9Y490|TLN1_HUMAN
1.22
3
3
200
Sulfide:quinone oxidoreductase
sp|Q9Y6N5|SQRD_HUMAN
8.67
3
3
176
177
178
182
183
184
185
186
187
188
198
Figure S1 Subcellular localization of identified O-GlcNAz modified proteins. (A-C) Cellular component GO of
O-GlcNAz modified proteins using probe Biotin-Diazo-Alkyne, Biotin-DIBO-Alkyne and O-GlcNAcylated proteins
database in dbOGAP. We performed the molecular function GO on the website: http://www.pantherdb.org/ and
analysis in software Excel.