Supporting Information
Direct Ionization of Biological Tissue for Mass Spectrometric
Analysis
Bin Hu, Ying- Han Lai, Pui-Kin So, Huanwen Chen,* and Zhong-Ping Yao*
Figure S1. Photos of experimental setup for DI analysis of plant tissue (a) and animal tissue
(b).
491.2 Na185
a) 100
O
519.3
HO
OH
%
b) 100
300
Na
%
HO
OH
135.1
OH
HO
800
900
●797
↓
○
18:3
18:3
O
[MGDG(18:3/18:3)+Na]+
797.5
300
400
185.1
500
600
OH
681
700
681.4
O
OH
347.1
800
900
O
O
O
O
681.4
%
OH
O
OH
18:3
519.3
405.2
243.1
797.6
347.1
[DGDG(18:3/18:3)+Na]+
-162
-Glu
405.2
959.6
797.6
185.1 243.1
200
300
400
500
600
m/z
1000
● 959
↓
○
18:3
O
O
m/z
1000
335.2
HO
0
100
700
519.3
519.3
243.1
243.1
185.0
HO
600
O
O
200
Na
c) 100
500
O
O
0
100
769.5
O
OH
16:3
O
519
[MGDG(18:3/16:3)+Na]+
400
185
O
OH
243.1
[C12 H20O10Na]+
[Glu+Na]+
307.2
185.0
200
●769
↓
○
O
O
[C9H16 O6Na]+
0
100
18:3
O
491
OH
700
800
900
m/z
1000
Figure S2. MS/MS spectra of major ions observed in DI spectrum of the spinach leaf.
Figure S3. Total ion chromatogram (a) and mass spectrum of phenophytina a (retention time
(tr): 38.1 min) (b), pyropheophorbide a (tr: 20.0 min) (c), OPDA/16:2 MGD or 18:2/dnOPDA
MGD (tr: 30.8 min) (d), DGDG (34:6) (tr: 25.8 min) (e), and MGDG (18:3/16:3) (tr: 27.9
min ) (f) obtained by LC-MS analysis of the spinach extract.
Figure S4. DI spectra of (a) chilli, (b) tea, and (c) radish root acquired on the triplequadrupole mass spectrometer with methanol/water (1/1) as the added solvent.
[PS(40:6)+K]+
a)
100
porcine liver
[PC(34:1)+Na]+
%
700
[PS(40:6)+K]+
832
849.31
873.9
763.31
691.91
721.39 747.17
674.44
619.60
912.9
750
800
850
900
950
0
600
m/z
1000
797.27 825.35
100
799.29
%
808
741.0
678.9
650
porcine kidney
[PC(38:4)+Na]+
758.0
634.9
783.29
100
[PC(36:2)+Na]+
781.9
0
600
e)
[PS(36:1)+H]+
820
[PC(34:1)+H]+
b)
[PS(38:4)+K]+
847.9
797.9 823.9
f)
porcine spleen
650
700
865.80
750
800
905.62
850
949.85
900
950
900
950
990.51
m /z
1000
830.34
100
784.27
Fish heart
806.28
846.34
849.31
%
%
781.41
756.15
759.40
630.64 656.77 681.57
0
600
c)
100
650
892.56
705.81 735.92
700
750
800
850
946.35
900
950
983.87
m/z
1000
825
porcine lung
783
636.76
0
600
650
708.93
672.81
743.14
700
955.62
750
800
Fish gill
807
799.4
%
%
849
761
761.4
683
635
649
0
600
d)
100
914
660
650
670
697
700
871
744
725
750
800
850
894
886
900
955
950
m/z
1000
0
600
h)
100
857.3
650
700
750
800
850
906.1
900
959.8965.2
950
m/z
1000
950
m/z
1000
798.1
Bovine muscle
782.1
824.7
799.1
%
%
772.6
810.7
770.7
756.7
0
600
846.5
726.4
796.6
782.7
807.4
829.4 845.3
757.3
975 994
798.6
porcine medulla
781.3
759.3
941
933
m /z
1000
783.4
100
g)
800
850
650
700
750
782.0
760.1
826.6
848.7
849.7
800
850
800.1
758.1
826.1
891.2
742.1
900
950
m/z
1000
0
600
650
700
750
800
850
900
Figure S5. DI spectra of various animal tissues with methanol/water (1/1) as the added
solvent. Spectra b, c and d were acquired on the triple-quadrupole mass spectrometer.