A
B
PI: 2646
PI: 10 786
K-mer depth
Figure S1. Estimation of Sogatella furcifera genome size based on flow
cytometry and 17-mer statistics.
(A) Estimation of Sogatella furcifera genome size based on flow cytometry.
Comparing with the Drosophila melanogaster (D. mel) genome, the C value
of the S. furcifera (S. fur) genome was estimated to be 0.75 pg. The genome
size of S. furcifera was estimated to be 733Mb.
(B) Estimation of S. furcifera genome size based on 17-kmer statistics.
In total 56.76 Gb of short (<1kb) paired-end genome sequencing reads were
used to generate the 17-mer sequences. The genome size of S. furcifera was
estimated to be 730 Mb based on the formula: 17-mer number / 17-mer
depth.
0
1
-lg(P-value)
2
3
4
5
G-protein coupled receptor signaling pathway
G protein-coupled receptor signaling pathway
ion transport
multicellular organismal development
Wnt receptor signaling pathway
protein
proteinhomooligomerization
homo-oligomerzition
oxygen transport
dicarboxylic acid transport
potassium ion transport
homophilic cell adhesion
adherens junction assembly
photosynthesis
G proteinadenylate cyclase-modulating G-protein
coupled
receptor
signaling
pathway
coupled
receptor
signaling
pathway
Figure S2. Gene Ontology enrichment analysis for genes in expression pattern 1.
0
-lg(P-value)
2
4
catecholamine
biosynthetic process
aromatic amino acid
family metabolic
process
Figure S3. Gene Ontology enrichment analysis for genes in expression pattern 2.
0
2
-lg(P-value)
4
6
8
10
12
metabolic process
oxidation-reduction process
oxidation–reduction process
transmembrane transport
proteolysis
regulation of catalytic activity
aromatic amino acid family metabolic process
'de novo' IMP biosynthetic process
de
novo IMP biosynthetic process
transport
carbohydrate metabolic process
L-serine biosynthetic process
cellular carbohydrate metabolic process
proline biosynthetic process
pentose-phosphate shunt
phototransduction
visual perception
nucleoside metabolic process
nucleotide catabolic process
lipid metabolic process
cellular amino acid metabolic process
sulfate transport
Figure S4. Gene Ontology enrichment analysis for genes in expression
pattern 3. The top 20 enriched terms are shown.
0
0.5
-lg(P-value)
1
1.5
2
nucleosome assembly
reciprocal meiotic recombination
DNA catabolic process,
endonucleolytic
chitin metabolic process
Figure S5. Gene Ontology enrichment analysis for genes in expression pattern 4.
0
1
-lg(P-value)
2
3
4
microtubule-based movement
glycolysis
glycerol metabolic process
positive regulation of apoptotic process
inositol catabolic process
protein metabolic process
tricarboxylic acid cycle
regulation of microtubule polymerization
or depolymerization
Figure S6. Gene Ontology enrichment analysis for genes in expression pattern 5.
0
1
2
-lg(P-value)
3
4
5
6
7
8
protein metabolic process
protein polymerization
microtubule-based process
glycolysis
negative regulation of biosynthetic process
tyrosine metabolic process
GTP catabolic process
proteolysis
cellular amino acid metabolic process
negative regulation of nucleotide metabolic
process
cell motility
intraflagellar transport
L-phenylalanine catabolic process
protein targeting
Figure S7. Gene Ontology enrichment analysis for genes in expression pattern 6.
0
5
-lg(P-value)
10
15
20
25
chitin metabolic process
homophilic cell adhesion
chitin catabolic process
response to oxidative
stress
oxidation–reduction
process
alcohol metabolic process
proteolysis
multicellular organismal development
oxidation-reduction process
microtubule-based process
carbohydrate metabolic process
regulation of transcription, DNA-dependent
dicarboxylic acid transport
steroid
hormone mediated signaling
signaling pathway
steroid
hormone-mediated
pathway
protein polymerization
Wnt receptor signaling pathway
tetrahydrofolate biosynthetic process
sphingomyelin catabolic process
neurotransmitter secretion
negative regulation of cell differentiation
ecdysis, chitin-based cuticle
Figure S8. Gene Ontology enrichment analysis for genes in expression pattern 7.
0
1
2
-lg(P-value)
3
4
5
6
lipid transport
DNA repair
cell-cell signaling
cell–cell signaling
microtubule-based movement
histidine catabolic process to glutamate and formamide
cysteinyl-tRNA aminoacylation
proteolysis
base-excision repair
rRNA processing
intracellular signal transduction
signal transduction
oxidation-reduction process
process
oxidation–reduction
response to stress
metal ion transport
phosphate ion transport
transcription from RNA polymerase III promoter
poly(A)+ mRNA export from nucleus
mitotic spindle assembly checkpoint
nucleus-associated proteasomal ubiquitin-dependent…
C-5 methylation of cytosine
Figure S9. Gene Ontology enrichment analysis for genes in expression
pattern 8. The top 20 enriched terms are shown.
7