A Minimal Peptide Sequence That
Targets Fluorescent and Functional
Proteins into the Mitochondrial
Intermembrane Space
Amir H. Faraji
October 27, 2007
Amir Faraji @ Wipf Group
1
10/29/2007
Mitochondrial Anatomy
http://www.life.uiuc.edu/crofts/bioph354/lect10.html
Amir Faraji @ Wipf Group
2
10/29/2007
Membrane Translocation
Cytoplasm
TOM
Intermembrane
Space
Amir Faraji @ Wipf Group
3
10/29/2007
Membrane Translocation
Cytoplasm
TOM
TIM
Mitochondrial
Matrix
Amir Faraji @ Wipf Group
4
10/29/2007
Membrane Translocation Assay
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
5
10/29/2007
Smac/DIABLO Protein
Smac/DIABLO Protein, Protein Data Bank
Amir Faraji @ Wipf Group
6
10/29/2007
Localization of Fusion Protein
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
7
10/29/2007
Reconstitution of EGFP Fluorescence
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
8
10/29/2007
Reconstitution of EGFP Fluorescence
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
9
10/29/2007
Hydrophobicity Analysis
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
10
10/29/2007
Viral Vectors
Amir Faraji @ Wipf Group
11
10/29/2007
Mutation of Peptide Sequences
 Intermembrane Space
 Intermembrane Space
 Intermembrane Space
 Non-Mitochondrial
 Non-Mitochondrial
 Non-Mitochondrial
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
12
10/29/2007
Mutation of Peptide Sequences
 Intermembrane Space
 Matrix
 Matrix
 Matrix
 Matrix
 Intermembrane Space
 Intermembrane Space
 Matrix
Ozawa et al. ACS Chemical Biology, 2007, 2(3), 176-86.
Amir Faraji @ Wipf Group
13
10/29/2007
Summary
RSVCSLFRYRQRFPVLANSKKRCFSELIKPWHKTVLTGFGMTLCAVPI
10
57
Therapeutic Targeting?
Amir Faraji @ Wipf Group
14
10/29/2007
Summary
Cytoplasm
TOM
Intermembrane
Space
Amir Faraji @ Wipf Group
15
10/29/2007