HAT
KAT3
HDAC
HDAC
KMT
KMT
PRMT
PRMT
KDM
H3K9me3
H3K4me3
H3K27me3
H3K36me1,2
ASH2L: important for trymetilation of H3 K4
MEN1
MEN1
MEN1
Molecular regulation of H3K4 trimethylation by
ASH2L,
a shared subunit of MLL complexes
Melissa M Steward1, Jung-Shin Lee1, Aisling O’Donovan2,Matt Wyatt1,
Bradley E Bernstein2 & Ali Shilatifard1,3
SEPTEMBER 2006 NATURE STRUCTURAL & MOLECULAR
BIOLOGY
Mistargeting of chromatin modifications occur as epigenetic lesions at MLL-AF4 target
regions
Guenther M. G. et.al. Genes Dev. 2008;22:3403-3408
©2008 by Cold Spring Harbor Laboratory Press
Model of MLL-AF4-mediated gene activation in leukemia
Guenther M. G. et.al. Genes Dev. 2008;22:3403-3408
©2008 by Cold Spring Harbor Laboratory Press
Figure 1. Model in which PRC2 responds to the chromatin environment to establish H3K27me domains. Repressive features of
constitutive heterochromatin including DNA methylation (5mC), H3K9me (K9me), and HP1 binding influence methylation of H3K27
by PRC2. Conver...
Elizabeth T Wiles, Eric U Selker
H3K27 methylation: a promiscuous repressive chromatin mark
Current Opinion in Genetics & Development, Volume 43, 2017, 31–37
Fig. 1. Evolution of PcG family. Expansion of the Polycomb genes occurs in vertebrates as the genome size increases.
Katelyn E. Connelly, Emily C. Dykhuizen
Compositional and functional diversity of canonical PRC1 complexes in mammals
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Volume 1860, Issue 2, 2017, 233–245
http://dx.doi.org/10.1016/j.bbagrm.2016.12.006
Human polycomb complexes functionally interact to regulate histone H3K27 methylation and
histone H2A K119 ubiquitination.
Emily C. Chittock et al. Biochm. Soc. Trans. 2017;45:193205
©2017 by Portland Press Ltd
Figure 1. Schematic representation of the various classes of PRC1 complexes. (A) Except for PCGF components, subunits are
represented in a simplified manner that precludes description of paralogs. Protein–protein contacts are not meant to be accurate in
all ca...
Miguel Vidal, Katarzina Starowicz
Polycomb complexes PRC1 and their function in hematopoiesis
Experimental Hematology, Volume 48, 2017, 12–31
The PRC1.
Emily C. Chittock et al. Biochm. Soc. Trans. 2017;45:193205
©2017 by Portland Press Ltd
SUZ12
Colon
Prostate
Colon
Breast
Breast
Skin
Liver
Lymphoma
Liver
Myeloma
Endometrial
Bladder
Lung
Gastric
EZH2
Proteins
Drosophila melanogaster
Protein domains
Human
Mouse
ESC
EED
EED
WD40 repeats*
E(Z)
EZH1
EZH1/ENX2
SET domain‡
EZH2
EZH2/ENX1
SUZ12
SUZ12
Zinc-finger domain
CBX2/HPC1
CBX2/M33
Chromodomain
CBX4/HPC2
CBX4/MPC2
CBX8/HPC3
CBX8/PC3
EDR1/HPH1
EDR1/MPH1/RAE28
EDR2/HPH2
EDR2/MPH2
EDR3/HPH3
EDR3
RING1/RNF1/RING1A
RING1/RING1A
RNF2/RING1B
RNF2/RING1B
BMI1
BMI1
RNF110/ZFP144/ PCGF2
RNF110/ZFP144/MEL18
ZNF134
ZNF134
YY1
YY1
Functions
PRC2 initiation complex
SU(Z)12
Histone methyltransferase
PRC1 maintenance complex
PC
PH
RING
PSC
PHO
Methyl-lysine binding
Zinc-finger SPM
domain§
RING-finger domain||
Ubiquitin ligase
RING-finger domain||
Zinc-finger domain
PHO-like
Sequence-specific DNA
binding
DNA binding
SCM
SCML1
SCMH1
Zinc-finger SPM
domain§
PCL
PHF1
PHF1/PCL1
PHD-finger domain
Global perturbations of histones PTMs in human cancers.
H4K20me3
Lymphoma
mouse SCC
Fraga et al. Nat Genet. 2005.
H4K16ac (MOF)
Lymphoma
Breast, Medulloblastoma
Fraga et al. Nat Genet. 2005.
H3K4me2 (MLL)
Prostate
Seligson et al. Nature. 2005.
H3K18ac
Prostate
Seligson et al. Nature. 2005.
H3K9me2
Prostate, Kidney
Seligson et al. Am J Pathol. 2009.
Ellinger et al. Prostate 2010.
H3K18ac
Lung, Kidney
Seligson et al. Am J Pathol. 2009.
Ellinger et al. Prostate 2010.
H3K27me3 (Pg)
Prostate
Yu et al. Caner Res. 2007.
Pfister ey al. Int J Cancer 2008.