Figure S1
Huang, Yi et al.
a-HDAC1
a-LSD1
IgG
Input
SAHA
a-HDAC1
a-LSD1
IgG
IP:
Input
Untreated
LSD1 (IB)
HDAC1 (IB)
Figure S1
H2O
5 mM
10 mM
SAHA
1 mM
A
Untreated
Figure S2
Huang, Yi et al.
LSD1
10 mM
5 mM
SAHA
1 mM
B
Untreated
Actin
LSD1
Actin
Figure S2
Table S1
Huang, Yi et al.
Table S1. Functional classification of genes whose expression is changed only by combined treatment with
SAHA and pargylinea.
Gene Function
Enrichment
Scoreb
Gene Namec
Mitosis, Cell division
3.08
Cyclin A2, cell division cycle associated 3, Cyclin F
RhoGAP, GTPase activation
2.01
Rho GTPase activating protein 26
G protein signaling
1.71
regulator of G-protein signaling 16, regulator of G-protein
signaling 4
sirtuin (silent mating type information regulation 2 homolog) 4 (S.
cerevisiae), heart and neural crest derivatives expressed 1,
chromobox homolog 5 (HP1 alpha homolog, Drosophila), atrophin
1, TAF9B RNA polymerase II, TATA box binding protein (TBP)associated factor, MYST histone acetyltransferase (monocytic
leukemia) 4
Negative regulation of
transcription
1.69
ANK, phosphoproteins
1.68
protein phosphatase 1, regulatory (inhibitor) subunit 12B
Transcription regulator
activity
1.52
nuclear receptor subfamily 4, group A (member 1), zinc finger,
MIZ-type containing 2, nuclear receptor subfamily 4 (group A,
member 3), pre-B-cell-leukemia homeobox 1,transcription factor
EB, cAMP responsive element binding protein 3-like 1, runtrelated transcription factor 1, distal-less homeobox 2, heart and
neural crest derivatives expressed 1, Kruppel-like factor 2 (lung),
MYST histone acetyltransferase (monocytic leukemia) 4
ATP binding, nucleotide
binding
1.52
mitogen-activated protein kinase kinase kinase 13, fibroblast
growth factor receptor 1, antigen identified by monoclonal
antibody Ki-67
Nucleosome assembly
1.48
histone cluster 1, MYST histone acetyltransferase (monocytic
leukemia) 4
Helicase activity
1.36
DNA replication helicase 2 homolog (yeast)
Transmembrane proteins
1.35
furry homolog (Drosophila), cAMP responsive element binding
protein 3-like 1
Neoplasm part, polymerase II
activity
1.32
TAF12 RNA polymerase II, stonin 1, TAF9B RNA polymerase II,
Endoplasmic reticulum
1.31
glycosyltransferase 25 domain containing 2
Guanyl nuclear binding
1.30
Rho family GTPase 1, ras-related C3 botulinum toxin substrate 3
(rho family, small GTP binding protein Rac3)
cysteine-rich protein 2, zinc finger protein 280A, ring finger
protein (transmembrane 2), jumonji C domain containing histone
demethylase 1 homolog D (S. cerevisiae), tripartite motifcontaining 14, PDZ and LIM domain 2 (mystique)
Zinc ion binding
1.22
Microtubule cytoskeleton
1.08
tubulin tyrosine ligase-like family, member 1
Protein catabolic process
1.08
zer-1 homolog (C. elegans), ubiquitin specific peptidase 20, cell
division cycle associated 3, ubiquitin specific peptidase 5
(isopeptidase T)
Nucleoside triphosphate
metabolic process
1.06
ATPase, Na+/K+ transporting, alpha 3 polypeptide, ectonucleoside
triphosphate diphosphohydrolase 3, ATPase, Cu++ transporting,
beta polypeptide
a.
b.
c.
The Database for Annotation, Visualization and Integrated Discovery (DAVID) Bioinformatics Resources 6.7 program is used.
Genes functions with enrichment score >1 are shown.
Listed are genes with expression induction or reduction equal or greater than 1.5 fold.