Production of a high quality of
biococktail from beneficial bacteria
RUTH
1Institute
1
SCHMIDT
AND AMR
2
MOSTAFA
for Environmental Biotechnology, University of Technology, Graz, Austria
2 Institute for Microbiology, University of Caio, Egypt,
Approach/Method of solution
Plant-associated microorganisms perform beneficial effects on plants via direct and indirect mechanisms. Microbes directly promote plant growth by improved nutrient acquisition and hormonal
stimulation. Several mechanisms are involved in the suppression of plant pathogens. The aim of this study was to test already isolated and characterized strains of plant-associated microorganisms that
could serve as biological control agents (BCAs) for a farm in Egypt. Six rhizospheric strains were selected for ad planta applications on German chamomile (Matricaria chamomilla L.). The best strains
are going to be selected for the production of a high quality biococktail improving the quality and quantity of organic crops as well as excluding hazard chemicals and pesticides at the same time.
Experiments and Results
3. Fresh and dry weight
Dry and fresh weight of chamomile blossoms were determined each seven days for three
times. Both, fresh and dry weight Serratia plymuthia 3Re4-18, Bacillus subtilis Co1-6 and
Paenibacillus polymyxa Mc5Re-14 showed the highest yield compared to the control (Figure 4
and Figure 5). According to the treatment, however, the content of moisture was different.
1. Bacterial inoculation
One week old seedlings of the German
chamomile (Matricaria chamomilla L.)
were inoculated with bacterial strains
Streptomyces
subrutilus
Wbn-11,
Bacillus subtilis Co1-6, Paenibacillus
polymyxa Mc5Re-14, Pseudomonas
fluorescens
L13-6-12,
Stenotrophomonas rhizophila P69 and
Serratia plymuthia 3Re4-18. According
to the orientation shown in Figure 1.
Figure 1: Orientation of bacterial strains at Adleya farm/Sekem, Egypt. Strain 1:
Streptomyces subrutilus Wb2n-11, Strain 2: Bacillus subtilis Co1-6, Strain 3:
Paenibacillus polymyxa Mc5Re-14, Strain 4: Pseudomonas fluorescens L13-6-12,
Strain 5: Stenotrophomonas rhizophila P69, Strain 6: Serratia plymuthia 3Re4-18,
Control: Water
2. Chemical and microbial analysis
Figure 4: percentage of fresh weight
For analysis of phyisco-chemical properties of the soil, electrical conductivity (E.C.), pH,
organic carbon (O.C.), organic matter, total nitrogen (T.N.), total phosphorus (T.P.) and
total potassium (T.K.) was determined from 35 samples, taken from each plot of the field
(Table 1).
Rhizospheric samples were analyzed for total bacterial and total fungal count (Figure 2).
Testing of arbuscular mycorrhiza (AMF) was performed by staining the roots with trypan
blue (Figure 3).
E.C µS/m
258.71
pH
8.47
O.C %
0.81
O.M %
1.42
T.N %
0.15
T.P %
0.10
Figure 5: percentage of dry weight
4. Compound identification
Quantitative estimation of the flavone apigenin-7 glucoside in florets was performed by High
Performance Liquid Chromatography. Chromatogram A shows a typical profile of Serratia
plymuthia 3Re4-18 with the identified apigenin-7-glucoside, indicated by an arrow. A difference
in the percentage of apigenin-7-glucoside could be detected. This fact shows, that bacteria have
an influence on the secondary metabolism of plants. The percentages of apigenin-7-glucoside of
all tested strains are shown in Figure 6.
T.K %
0.07
Table 1: phyisco-chemical properties of the soil
Figure 2: bacterial diversity in the rhizosphere
Figure 3: stained arbuscular mycorrhiza (AMF)
Chromatogram A: typical apigenin-7-glucoside profile of Serratia plymuthia 3Re4-18
Figure 6: percentage of apigenin-7-glucoside
Outlook
In further experiments, main components of blue essential oil are going to be examined by GC/MS. Bacterial diversity and amount of bacterial DNA will be analyzed using 16S rDNA based singlestranded conformation polymorphism analysis (SSCP) fingerprint and quantitative real-time polymerase chain reaction (qPCR).
According to this data, production of a high quality biococktail using different culturing processes such as batch, fed-batch and continuous cultures will be conducted. Different dilutions rates will be
used to obtain a high product of bacterial biomass with high quality. Furthermore, different parameters such as such as oxygen uptake, CO2-outlet, oxygen consumption rate, pH values and sugar
consumption rate are going to be elucidated.
MENA Supervisor:
Prof. Dr.
Elshahat M. Ramadan
Microbiology Dept.
MENA Student: Amr Mostafa
Student picture
European Supervisor:
Dr.rer.nat. Gabriele Berg
Institute for Environmental
Biotechnology
European Student: Ruth Schmidt
This project has been funded with support from the European
Commission.
This poster reflects the views only of the author, and the Commission
cannot be held responsible for any use which may be made of the
information contained therein.
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