Product Description List LS-45 & LS-55 Luminescence Spectrometers Table of Contents Page Introduction ...................................................................................................................................... 2 LS-55 Fluorescence Spectrometer .................................................................................................. 3 LS-45 Fluorescence Spectrometer 4 Sampling Accessories ...................................................................................................................... 5 Thermostated Cell Holders 5 Well Plate Reader Accessory 5 Total Emission Accessory 6 Front Surface Accessory 6 LC Flow Cell Accessory 6 Sipper Cell and Pump Accessory 6 Low-temperature Accessory 6 Remote Fiber Optic Accessory 6 Fluorescence Liquid Autosampler 7 Polarization 7 Software Upgrades and Add-On Software Applications .................................................................. 8 Intracellular Ion Measurements ........................................................................................................ 9 Intracellular lon Filter Guide 9 Intracellular Ion Accessory 9 Intracellular Ion Filter Sets 9 Cells for Fluorimetry ....................................................................................................................... 10 Reference Materials and Test Samples ......................................................................................... 10 Sources, Detectors, Attenuators, Filters and Septa ....................................................................... 11 Recommended Minimum Computer Configuration ........................................................................ 11 PerkinElmer Life and Analytical Sciences 710 Bridgeport Avenue Shelton, CT 06484-4794 U.S.A. (800) 762-4000 or Tel.:(+1) 203-925-4602 Fax: (+1) 203-944-4902 FLR-PDL9 Product Description List is subject to change without notice. February 2009 1 Introduction Fluorescence Spectroscopy is an analytical technique used for a wide variety of applications including cuvette based and plate based measurements. PerkinElmer offers accessories and software that are designed to satisfy the needs of the many different application fields. The LS-55 is the gold standard for automated chemical and biological research. It is a scanning Fluorescence Spectrometer covering the wavelength range of 200–800 nm with the excitation monochromator and 200–900 nm with the emission monochromator. The instrument has variable slits on both the excitation (2.5 to 15nm) and emission (2.5 to 20 nm) monochromators. The instrument can measure fluorescence, time resolved fluorescence, phosphorescence and luminescence. Therefore this instrument can be used for a wide range of applications and accessory options and serves as a base for the application systems. The LS-45 provides affordable performance for routine analyses. It is a scanning Fluorescence Spectrometer covering the wavelength range of 200–800 nm with the excitation monochromator and 200–900 nm with the emission monochromator. The instrument has fixed slits (10 nm) on both the excitation and emission monochromators. Both instruments are fully computer controlled systems using FL WinLab. FL WinLab, a Windows based software package, controls the instrument, sampling accessories, data collection and data processing. The software provides specific applications such as Scan, Synchronous scanning, Time Drive, Ratio Data Collection, ICBC Calibration, Well Plate Reader and 3D view. FLR-PDL9 Product Description List is subject to change without notice. February 2009 2 LS-55 Fluorescence Spectrometer Application: Fluorescence spectrometer that can be configured with software and accessories to address a wide range of application requirements. L2250107 (220-240V) L2250117 (110V) Description: Versatile, computer controlled Fluorescence spectrometer; incorporates a 8.3 watt, pulsed xenon source for fluorescence and phosphorescence decay time measurements. Excitation 200–800 nm and emission 200–900 nm with zero order selectable. Standard PMT covers 200–650 nm; R928 or R955 PMT optionally available for full range. Synchronous scanning with constant wavelength or frequency difference included. Excitation spectra are automatically corrected. Uses holographic excitation grating for reduced stray light levels. Variable excitation 2.5–15 nm and emission 2.5 to 20 nm slits in 0.1 nm increments. Scan speeds can be selected in 1 nm increments from 101500 nm/minute. Includes Single-position, water-thermostatted cell holder and Septum injector Incorporates a software-controlled filter wheel in the emission monochromator with 290, 350, 390, 430 and 515 nm cutoff filters, 1% T attenuator and clear beam. Includes automated polarization accessory that consists of 2 filter wheels; each wheel containing a horizontal and vertical polarizing element. Polarizer positions are software controlled and can be manually set or automatically controlled for polarization, anisotropy or G-factor Includes sample compartment purge nipple to allow dry air or nitrogen to be blown into the sample area to prevent the optics fogging for low temperature applications. FL WinLab Software Package, Windows based software controls the instrument, sampling accessories and data collection. The software provides specific applications such as Scan, Time Drive, Ratio Data Collection, ICBC Calibration, Well Plate Reader and 3D view. 1V and 10 mV analog output. L2250107 Configured for operation with 220-240 VAC, 50/60 Hz. L2250117 Configured for operation with 110 VAC, 50/60Hz System Components: LS-55 Spectrometer including Users Guide FL WinLab Software including Users Guide RS232C Cable Single-position, water-thermostatted cell holder Not Included B0631104 B0631107 FLR-PDL9 Personal Computer and Printer – See computer section for configurations Recommended Consumables and Spare Parts for the LS-55: Rectangular Cell, OS, 10 x 10 mm, PTFE Lid - 10 x 10 mm pathlength, Special Optical Glass, 3.5 mL volume. Set of 2 Rectangular Cell, QS, 10 x 10 mm, PTFE Lid - 10 x 10 mm pathlength. In Far UV fused silica, Quartz SUPRASIL, 3.5 mL volume. Set of 2. Product Description List is subject to change without notice. February 2009 3 LS-45 Fluorescence Spectrometer Application: Luminescence spectrometer that can be configured with software and accessories to address a wide range of application requirements. The LS-45 spectrometer is NOT suitable for polarization and Bioluminescence measurements. L2250106 (220-240V) L2250116 (120V) Description: Versatile, computer controlled Fluorescence spectrometer; incorporates a 9.9 watt, pulsed xenon source for fluorescence and phosphorescence decay time measurements. Excitation 200–800 nm and emission 200–900 nm with zero order selectable. Standard PMT covers 200–650 nm; R928 or R955 PMT optionally available for full range.. Synchronous scanning with constant wavelength or frequency difference included. Excitation spectra are automatically corrected. Fixed excitation and emission slits (10 nm). Scan speeds can be selected in 1 nm increments from 10-1500 nm/minute. Manual 1%T attenuator for signal attenuation and validation FL WinLab Software Package, Windows based software controls the instrument, sampling accessories and data collection. The software provides specific applications such as Scan, Time Drive, Ratio Data Collection, ICBC Calibration and 3D view. 1V and 10 mV analog output. L2250106 Configured for operation with 220-240 VAC, 50/60 Hz. L2250116 Configured for operation with 110 VAC, 50/60Hz System Components: LS-45 Spectrometer including Users Guide FL WinLab Software including Users Guide RS232C Cable Single-position, water-thermostatted cell holder L2250162 Accessory PCB Kit for the LS-45. Allows the LS-45 to be used with the PTP-1 (L2250150), biokinetic (L2250145) and Sipper (L2250135) accessories Requires installation by a service engineer Not Included Personal Computer and printer– See computer section for configurations B0631104 Recommended Consumables and Spare Parts for the LS-45: Rectangular Cell, OS, 10 x 10 mm, PTFE Lid - 10 x 10 mm pathlength, Special Optical Glass, 3.5 mL cell volume. Set of 2 B0631107 FLR-PDL9 Rectangular Cell, QS, 10 x 10 mm, PTFE Lid - 10 x 10 mm pathlength. In Far UV fused silica, Quartz SUPRASIL, 3.5 mL volume. Set of 2. Product Description List is subject to change without notice. February 2009 4 Sampling Accessories Thermostated Cell Holders L2250140 Single position thermostated Cell Holder - Cell holder for a 10 mm pathlength cell, water thermostated. This cell holder is included with the instrument. L2250141 Single position thermostated Cell Holder - Cell holder for a 10 mm pathlength cell, water thermostated with stirrer. . Includes 6 stirrer fleas (04978499). L2250134 Four position Thermostated Automatic Cell Changer with Stirrer for LS55 - Cell holder accommodates four 10 mm pathlength cell or four microcells with four adapters (L2250139). All positions are stirred simultaneously. Includes six stirrer fleas (04978499). This accessory is NOT compatible with the LS-45 L2250145 Bio-Kinetic Accessory - Magnetically stirred single cell holder; water thermostated. Includes a built-in temperature sensor (range 0 to 100 C) and an event marker. Thermostatable by means of an external water bath (not included). Accommodates 10 mm pathlength cuvettes. Includes 6 stirrer fleas (04978499). If stirring capability is required, LS-45 requires accessory PCB kit (L2250162) L2250150 PTP-1 Single Position Research Peltier Controller for fluorescence. Temperature range 0 to 100 C. Includes Biokinetic Accessory (L2250145) which includes stirring, event marking and temperature sensing as an integral part of the accessory as well as controller box. Control is either via the keypad or TempScan software (L225B009). Please note that this accessory requires a source of liquid cooling such as the PCB 150 Peltier Chiller Bath (N1015158). Voltage supply selectable between 110 and 240V If stirring capability is required, LS-45 requires accessory PCB kit (L2250162) N1015158 PCB 150 Peltier Chiller Bath. Can be used either with the Water thermostated cell holders (L2250140, L2250134, L2250145) or as a cooling device for the PTP-1 Research Peltier (L2250150) Temperature range 20 to 60 C 04978499 Magnetic Stirrer Fleas - Package of 6. Well Plate Reader Accessory L2250042 Well Plate Reader Accessory - For measuring fluorescence or phosphorescence in 6,12-, 24-, 48-, or 96-well plates or for luminescence microdensitometry of TLC plates and electrophoresis gels. Controlled by the computer. Silica excitation and emission fibers for measurement above 260nm excitation and emission. Includes one pack of white microplates. Plate reader provides top reading facility only and can be used for fluorescence and time-resolved fluorescence/ phosphorescence. It cannot be used to measure chemior bio- luminescence. This accessory is NOT compatible with the LS-45 6005290 Optiplate 96 well plate with flat bottom, white. Pack of 50 6005299 Optiplate 96 well plate with flat bottom, white. Pack of 200 6005270 Optiplate 96 well plate with flat bottom, black. Pack of 50 6005279 Optiplate 96 well plate with flat bottom, black. Pack of 200 FLR-PDL9 Product Description List is subject to change without notice. February 2009 5 Total Emission Accessory L2250101 Total Emission Accessory - Enhances sensitivity by automatically placing a mirror in front of the emission grating. Essential for high sensitivity bio- and chemiluminescence measurements using cuvette holder. Requires, but does not include installation by a PerkinElmer Service Engineer (N0207173). This accessory is NOT compatible with the LS-45 N0207173 Service Installation Front Surface Accessory 52123130 Front Surface Accessory - For flat samples, powders and turbid samples. Includes two Powder Sample Holders (52123164). Adjustable for optimizing sensitivity. 52123164 Powder Sample Holder - Used with front surface accessory (52123130). 52123814 Synthetic Fused Silica Window - For Powder Sample Holder LC Flow Cell Accessory L2250138 LC Flow Cell Accessory – Includes 1.3 mm square section, 16 L volume synthetic fused silica flow cell (L2151247), 0.4 mm FEP inlet tube (L2151202); and 0.7 mm FEP outlet tube (L2151203). L2151202 0.4 mm ID FEP Inlet Tube to Flow Cell – Used as inlet tube for L2250138. Package of five. L2151203 0.7 mm ID FEP Tube - Used as outlet tube for L2250138 and inlet tube for L2250135. Sipper Cell and Pump Accessory L2250135 Sipper Cell and Pump Accessory for the LS-55 – Includes 1.5 mm square section, 25 L volume synthetic fused silica flow cell (L2251223) and computer controlled peristaltic pump. Fitted with 0.7 mm FEP inlet tube (L2151203) and 1.0 mm FEP outlet tube (L2151204). Uses pump tubing 04974155. For LS-45 requires accessory PCB kit (L2250162). Requires, but does not include, installation by a PerkinElmer Service Engineer (N0207173). L2151203 0.7 mm ID FEP Tube - Used as outlet tube for L2250138 and inlet tube for L2250135. L2151204 1.0 mm ID FEP Tube - Used as outlet tube to L2250135 N0207173 Service Installation for Sipper Accessory Low-temperature Accessory L2250136 Low-temperature Accessory - For measuring fluorescence or phosphorescence at liquid nitrogen temperature. Includes a pack of five sample tubes (52126027). This accessory is not compatible with the LS-45 52126027 Low-temperature Sample Tubes - For Low-Temperature accessory. Pack of five. Remote Fiber Optic Accessory L2250144 FLR-PDL9 Remote Fiber Optic Accessory – Allows you to take the measurement at the sample rather than taking the sample to the instrument. Includes bifurcated synthetic fused silica fiber optics (1 meter long, 280 to 800 nm range) and optical mount to spectrometer. Product Description List is subject to change without notice. February 2009 6 Fluorescence Liquid Autosampler L2250156 Fluorescence Liquid Autosampler – Allows user to make unattended measurements of liquid samples. Includes S10 liquid autosampler (N2020010), autosampler software (L225B004) and a semi-micro flow-through cell (B0631133). 09410077 USB to RS232 Adapter Provides additional RS232 port utilizing a USB port on the PC B3140617 S10 / AS-93Plus Autosampler Sample Tray Sample tray with 90 locations (13mm diameter) for 6ml or 8ml sample tubes. B3140618 S10 / AS-93Plus Autosampler Sample Tray Sample tray with 60 locations (16mm diameter) for 15ml sample tubes. B3140621 S10 / AS-93Plus Autosampler Sample Tray Sample tray with 21 locations (30mm diameter) for 50ml sample tubes location for a wash tube. and 1 B3001647 S10 / AS-93Plus Autosampler Sample Tray Sample tray with 29 sample locations (16mm diameter) for 15ml sample Tubes, 9 locations (30mm diameter) for 50ml sample tubes and 1 location for a wash tube. B0193235 Sample Tube, 6mL, Package of 500. Polypropylene with screw cap. B0508901 Sample Tube, 8mL, Package of 1000. Polypropylene without screw cap. B0193233 Sample Tube, 15 mL. Package of 500. Polypropylene with screw cap. B0193234 Sample Tube, 50mL. Package of 500. Polypropylene with screw cap. Polarization The LS-45 is NOT suitable for polarization measurements With the LS-55, polarizations measurements can be performed with two different system configurations. The first configuration takes a measurement every 10 sec and is included as standard with the LS-55. The second configuration takes a measurement every 40 msec. and requires modification of the LS-55 for fast filters The LS-55 fast filter configuration consists of 1 x N1870195 Fast Filter Accessory Kit, 1 x Fast Filter Accessory Drive Unit, 1 x L2250030 Polarization Filter Kit plus installation. N1870195 FLR-PDL9 Fast Filter Accessory Kit – Includes the Fast Filter Accessory Board and Software (L2250019) and one drive unit (L2250146). Requires, but does not include Polarization Filter Kit (L2250030) and the Fast Filter Accessory Drive Unit (L2250146). Requires, but does not include installation by a PerkinElmer Service Engineer (N0207177) Please note that the polarizer drive must be removed from the LS-55 to accommodate this accessory (the L2250030 polarizer elements can be mounted in two drive units) Product Description List is subject to change without notice. February 2009 7 L2250146 Additional Fast Filter Accessory Drive Unit - Required for updating N1871095 to allow polarization or both excitation and emission filters to be used. Requires filter set. Requires, but does not include installation by a PerkinElmer Service Engineer (N0207173). L2250030 Fast Filter Polarization Filter Kit - Consists of two (2) pairs of polarizing filters each set at 0 and 90 degrees to the vertical. Enables polarization values to be measured every 40 msec. Requires two (2) drive units (L2250146). Installation by a PerkinElmer Service Engineer is included unless a drive unit is required. N0207177 Service Installation for the Fast Filter Accessory Kit (N1870195). N0207173 Service Installation for the Fast Filter Accessory Drive Unit(s) (L2250146) or Excitation and emission polarizers (L2250100) Software Upgrades and Add-On Software Applications L2258000 FL WinLab Version 4.00.02 Software – For registered users of earlier versions of FL WinLab Windows based software controls the instrument, sampling accessories and data collection. The software provides specific applications such as Scan, Time Drive, Ratio Data Collection, ICBC Calibration, Well Plate Reader and 3D view. Included in LS50B computer-ready, PC systems and specified update kits. Includes Software User´s Guide. L225B004 Auto Sampler (ASP) Application takes luminescence intensity, polarization, or anisotropy values from samples acquired with the Perkin-Elmer AS93 plus or S10 autosampler accessory. All data acquisition, autosampler and sipper (if fitted) accessory control is coordinated by the application. Application methods specify autosampler vial set and order of sampling, pump control options including return of precious samples to vial after measurement and rinsing between samples. The results are stored in TAB-delimited text format convenient for third-party post-run processing software (e.g. spreadsheets). Concentration data may be entered for any standard samples for later calculation of concentrations for unknowns by third-party software L225B005 Short Phosphorescence Decay Routine for collecting decay data and calculating single or dual exponential lifetimes. L225B009 TempScan software for use with the L2250150 PTP-1 Accessory only. Allows excitation, emission or synchronous scans to be collected at regular temperature increments. Data produced are single scans as well as a 3d data set which allows a curve of fluorescence intensity at a particular wavelength to be plotted against temperature. For highest accuracy, the external sample temperature probe accessory (L2250147) is recommended. FLR-PDL9 Product Description List is subject to change without notice. February 2009 8 Intracellular Ion Measurements Intracellular lon Filter Guide Filter/Dye nm/nm Mono. Ion FURA-2 340/380 Excitation Ca++ Or 340/365 Excitation Ca++ with IBP Mag-FURA-2 340/380 Excitation Mg++ BCECF 495/440 Excitation pH SNAFL-2 514/550 Excitation pH INDO-1 405/480 Emission Ca++ SNARF-1 580/640 Emission pH If using 2 filter sets which use the same monochromator, for example FURA2 and BCECF, only one (1) drive unit (L2250146) is required. Both dyes can be monitored simultaneously. If using 2 filter sets which use different monochromators, for example BCECF and INDO-1, two (2) drive units are necessary - one for excitation and one for emission. The 2 dyes cannot be measured simultaneously. Intracellular Ion Accessory N1870195 Fast Filter Accessory Kit – Includes the Fast Filter Accessory Board and Software (L2250019) and one drive unit (L2250146). Requires, but does not include a filter set (see below). Requires, but does not include installation by a PerkinElmer Service Engineer (N0207177) This accessory is NOT suitable for the LS-45 N0207177 Service Installation for the Fast Filter Accessory Kit. L2250146 Fast Filter Accessory Drive Unit – Second drive unit, Requires Fast Filter Accessory Kit (N1871095) to allow both excitation and emission filters to be used. Requires filter set. Requires, but does not include installation by a PerkinElmer Service Engineer (N0207173). N0207173 Service Installation for the Fast Filter Accessory Drive Unit(s) Intracellular Ion Filter Sets L2250021 Filter Set - FURA 2 (Excitation) L2250022 Filter Set – BCECF (Excitation) L2250023 Filter Set - SNAFL-2 (Emission) L2250024 Filter Set - INDO-1 (Emission) L2250025 Filter Set – SNARF-1 (Emission) FLR-PDL9 Product Description List is subject to change without notice. February 2009 9 Cells for Fluorimetry B0631104 Rectangular Cell, OS, 10 x 10 mm, PTFE Lid - 10 x 10 mm pathlength, Special Optical Glass, 3.5 mL cell volume. Set of 2. B0631107 Rectangular Cell, QS, 10 x 10 mm, PTFE Lid - 10 x 10 mm pathlength. In Far UV synthetic fused silica, Quartz SUPRASIL, 3.5 mL cell volume. Set of 2. B0631110 Rectangular Cell, OS, 10 x 10 mm, with PTFE Stopper - 10 x 10 mm pathlength, Special Optical Glass, 3.5 mL cell volume. Set of 2. B0631113 Rectangular Cell, QS, 10 x 10 mm, with PTFE Stopper -10 x 10 mm pathlength, Quartz SUPRASIL, 3.5 mL cell volume. Set of 2. B0631124 Ultra-Micro Cell, QS, 10 x 2 mm, with PTFE Stopper - 10 x 2 mm pathlength, Quartz SUPRASIL, Chamber volume 100 µL. Fill volume 120 L. B0631123 Micro Cell, QS, 5 x 5 mm, without Lid - 5 x 5 mm pathlength, Quartz SUPRASIL, 600 µL cell volume. Set of 2. Requires micro cell adapter (L2250139). B0631142 Micro Cell, QS, 5 x 5 mm, with PTFE Stopper NS 5 - 5 x 5 mm pathlength, Quartz SUPRASIL, with PTFE Stopper NS 5, 850 L cell volume. Set of 2. Requires micro cell adapter (L2250139). L2250139 Micro Cell Adapter - Adapter allows 5 mm pathlength micro cells to be used in any of the cell holders. Package of 4. B0631132 Semi-Micro Cell, magnetically stirred, QS, 4 x 4 mm - 4 x 4 mm pathlength, Quartz SUPRASIL. Used in any stirred cellholder, 500 µL cell volume, requires stirrer fleas, (04978499). L2250008 Coverslip Holder Accessory – Designed for 10 mm round coverslips with thickness of 0.155 to 0.185 mm. Package of 4. Requires (but does not include) a 10 mm pathlength cell with lid (not stopper). B0631125 Semi-Micro Cell, QS – 10 x 4 mm, with PTFE Stopper, Temperature Controlled. 10 x 4 mm pathlength, Quartz SUPRASIL, with PTFE Stopper, 1.4 mL cell volume. Horizontal inlet/outlet tubes for thermostatting. B0631133 Semi-Micro Flow-Through Cell, QS, - 3 x 3 mm, Screw fitting Inlet/Outlet Tubes 3 x 3 mm pathlength, Quartz SUPRASIL, 100 µL cell volume. Compact with 2 screw connectors and FEP tubing. Reference Materials and Test Samples 52019600 Luminescence Sample Blocks –Set of six, including Europium for testing phosphorescence 1 = Anthracene + Naphtalene (360+290/402+330 nm), 2 = Ovalene (342/482 nm), 3 = p-Terphenyl (295/338 nm), 4 = Tetraphenyl-butadien (348/422 nm), 5 = Europium (Phosphorescence, 335/613 nm) 6 = Rhodamin B (562/573 nm) 52124965 Phosphorescence Sample Block Included in 52019600. L2251293 Sealed water cuvette - Sealed 10 mm pathlength cell containing high purity water for Raman band measurements. CP364460 Ovalene sample block. Part of the 52019600 set. FLR-PDL9 Product Description List is subject to change without notice. February 2009 10 Sources, Detectors, Attenuators, Filters and Septa L2251157 Replacement Pulsed Xenon Source for LS-45 and LS-55 (can also be used with the LS30, LS40, LC240 and LS50/50B instruments) 04969486 Replacement Festoon bulb for LS-45 and LS-55 (can also be used with the LS30, LS40, LC240 and LS50/50B instruments) 52124966 R928 Red-sensitive sample Photomultiplier (PMT) – For measurements up to 900 nm. L6020281 R955 Red-sensitive sample Photomultiplier (PMT) - For measurements up to 900 nm. This PMT offers better performance in the UV (200-250 nm) than the R928 C6850012 Diffuser plate - For carrying out emission correction between 200nm and 350nm. 52126319 Attenuator, 1%Transmission 50mm square.– Included with LS-45; 52126320 Attenuator, 2%Transmission 50mm square. 52123926 Attenuator 8% Transmission 50mm square 52123887 Attenuator 33% Transmission 50mm square 02639507 Manual Cut-Off Filter 290nm, 5x5cm – Wavelength filter Included in LS-55 filter wheel 02639508 Manual Cut-Off Filter 310nm, 5x5cm 02639505 Manual Cut-Off Filter 350nm, 5x5cm - Wavelength filter Included in LS-55 filter wheel 02639509 Manual Cut-Off Filter 390nm, 5x5cm - Wavelength filter Included in LS-55 filter wheel L2151164 Manual Cut-Off Filter 530nm, 5x5cm L2251286 Septum Injector Body – Included with LS-55 L2251287 Septum Injector Cap .– Included with LS-55 04961071 Spare septa - For standard septum injector and Luminometer accessory L2250037. Package of 3. Recommended Minimum Computer Configuration Minimum processor speed Intel Core 2 Duo Minimum RAM 1.0 GB (2 x 512MB) Minimum hard disk space 160 GB Screen resolution 1024 x 768 SVGA CD ROM / Floppy disk required ? Yes - CD-RW / No - Floppy Communications RS232-C Operating systems supported Windows XP Professional SP2 Other software required FL WinLab 4.00.03 Please note that PerkinElmer reserves the right to supply equivalent or enhanced versions of products resold by PerkinElmer, such as computers, in response to model changes by the manufacturer. FLR-PDL9 Product Description List is subject to change without notice. February 2009 11
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