INNOVATIONS
IN EMERGING POLLUTANTS
OCEAN MONITORING
USING BIOSENSORS
Dr. Ing. L. Sanfilippo – SYSTEA S.p.A., Italy
Sequential
automated
methods
improved in
µMac-Smart
instrumental
prototype
ALGAL TOXINS
Automated method
developed and under
integration into
µMac-Smart
instrumental prototype
after automatic
sampling,
preconcentration and
cells lysis
TOXIC ALGAL SPECIES
Automated
method improved
into µMac-Smart
& WIZ probe
prototypes and
coupled with
automatic
sampling and
Solid Phase
Extraction
preconcentration
PHARMACEUTICALS
WIZ
multiparametric
nutrient probe for
these nutrients
was extensively
tested on the
coastal buoy
NUTRIENTS
FLOATING PLATFORM
LA SPEZIA - ITALY
Modular measurement prototypes:
WIZ probe
COASTAL BUOY
PIRAN - SLOVENIA
Prof. Giuseppe Palleschi
Università degli Studi di Roma Tor Vergata
[email protected]
http://www.project-sms.eu
ALGAL TOXINS DETECTION
IN SEA WATER
PRINCIPLE
ALGAL TOXINS DETECTION
IN SEA WATER
µMac-Smart module based on
Enzyme Linked Immuno-Magnetic
Optical (ELIMO) competitive
assays for the quantitative
sequential measurement of:
Okadaic acid
Domoic acid
Saxitoxin.
Typical reaction volume: 500 µL
Minimum reagents volume: 20 µL
The automated measurement module is capable
to measure unattended up to 50 water samples.
AUTOMATED ALGAL
TOXINS DETECTION
TOXIC ALGAE DETECTION
IN SEA WATER
PRINCIPLE
TOXIC ALGAE DETECTION
IN SEA WATER
µMac-Smart module based on
Enzyme Linked ImmunoMagnetic Optical (ELIMO) assays
is able to detect microalgae
from genera Alexandrium,
Dinophysis and Pseudo-nitzschia
The water sample is automatically
pretreated by a separated
automated module performing
cells preconcentration and lysis.
Typical sample volume: 200 µL
Minimum reagents volume: 20 µL
CELLS PRECONCENTRATION
AND LYSIS
AUTOMATED MODULE
Cells pre-concentration using an hollow
fibre filter, 0.1 microns cut-off,
specifically tailored for the application
Concentration factor x 2000
Reuse: 10 x without loosing recovery
Cells lysis of the pre-concentrated water
sample by ultrasounds
Positive tests with lysate able to detect
down to 5000 cells / L of Alexandrium
minutum spiked in environmental
sample (actual LOD).
Dimensions:
50 (H) x 40 (W) x 20 (D) cm
Calibration tests
Spiked SeaWater with Alexandrium
minutum cells
TOXIC ALGAE DETECTION
IN SEA WATER
Laboratory tested @ SYSTEA (Dec-Mar)
Blanc
PC
Laboratory tests
@ MICROBIA
(April-May)
0.6
0.45
0.3
µMac-Smart
for Toxic Algae Detection
0.15
0
SYS
Tests with
prototype
MIC
MIC
Manual Test
Fully automated Integrated system for
toxic algae detection:
From pre-concentration to detection
+
•
Detection of Alexandrium minutum (AM)
and PseudoNitzchia spp.(PN) in Seawater
Lysates
•
Shown in equivalent cell numbers
SULPHONAMIDE
DETECTION IN SEA WATER
+
To achieve sensitive
environmental
concentrations Solid Phase
Extraction automated
preconcentration device
is still under R&D
NUTRIENTS DETECTION IN
SEA WATER
WIZ in-situ multiparametric
probe was installed in the Vida
coastal buoy, to measure
nutrients (NH3, NO3+NO2, NO2
and PO4), including 0.1 µm cutoff automatic filtration &
autocleaning
NUTRIENTS DETECTION IN
SEA WATER
http://nitlab.inf.uth.gr/
smsgui/charts.html
INTEGRATED WATER QUALITY
MONITORING SYSTEM AND
DATA MANAGEMENT
Local data
acquisition &
remote
communication
3G / UMTS
WiFi to shore
side gateway
INTEGRATED MONITORING
SYSTEM AND DATA
MANAGEMENT (2)
A large coastal water buoy already in
operation in Piran (Slovenia) is
hosting the equipment to measure
the following parameters:
Toxic algae species (Alexandrium
Minutum, Pseudonitzschia or
Dinophysis)
The field monitoring data
are accessible from a
remote Web server
Dissolved nutrients (NH3, NO3,
NO2, PO4) by WIZ in-situ probe +
0.1 µm cut-off filtration &
autocleaning
Standard water quality parameters
(Temperature, EC, pH, DO,
Turbidity, Chlorophyll)
INTEGRATED WATER QUALITY
MONITORING SYSTEM AND
DATA MANAGEMENT (3)
The field monitoring
data are going to be
available in the same
remote Web server
A relocatable floating platform is
going to be deployed next week in
the bay of La Spezia (Italy), for field
monitoring of the following
dissolved compounds (after 0.1 µm
cut-off filtration with automatic
cleaning by filtered sample
backwash):
Domoic acid, Okadaic acid and
Saxitoxin
Standard water quality
parameters (Temperature, EC,
pH, DO, Turbidity, Chlorophyll)
CONCLUSIONS
Innovative analytical methods were developed and fully
automated, in the frame of the European funded research
project SMS (http://www.project-sms.eu), to measure
toxic algae, their related algal toxins and sulfonammides,
then coupled with nutrients detection in coastal water
Toxic algae and algal toxins methods are based on the
same ELIMO µLFR analytical technology
Field tests are just starting in Slovenia and Italy and they
will run until the beginning of August
Lab data comparisons and validation are going to be
performed during the field tests
The ELIMO analytical concept allows its adaptation to
detect other chemical and biological targets for coastal
and even fresh water.