CC founder strains: body weight
CC founder strains: glucose during oGTTs
CC founder strains: insulin during oGTTs
CC founder strains: glucose during ITTs
Co-expression gene modules in F2 islets
~33,000 transcripts
~20,500 in modules
“cell cycle”
(184 transcripts)
Module enrichments by GO
module
GO
GO.term
black
blue
brown
cyan
darkgreen
darkgrey
darkred
darkturquoise
green
greenyellow
grey60
lightcyan
lightgreen
lightgreen
lightgreen
lightgreen
lightgreen
lightgreen
lightyellow
magenta
midnightblue
orange
pink
purple
red
royalblue
salmon
tan
turquoise
yellow
BP
BP
BP
CC
BP
CC
BP
BP
CC
MF
BP
BP
BP
BP
BP
BP
BP
BP
BP
BP
BP
BP
BP
BP
CC
BP
CC
MF
MF
BP
positive regulation of T cell cytokine production
chromatin modification
fructose 1,6-bisphosphate metabolic process
mitochondrial respiratory chain complex I
carbon dioxide transport
polysome
fructose 2,6-bisphosphate metabolic process
adiponectin-mediated signaling pathway
proton-transporting V-type ATPase, V1 domain
chemokine activity
antigen processing and presentation of endogenous peptide antigen via MHC class I
translational elongation
nuclear division
mitosis
M phase of mitotic cell cycle
M phase
organelle fission
cell cycle phase
neutrophil mediated immunity
maternal process involved in female pregnancy
regulation of lipoprotein metabolic process
NAD metabolic process
detection of light stimulus involved in visual perception
otic vesicle formation
organellar large ribosomal subunit
DNA replication, synthesis of RNA primer
zymogen granule membrane
extracellular matrix structural constituent
neuropeptide hormone activity
RNA splicing
p.value
0.00000184
6.88E-18
8.16E-08
4.32E-111
1.62E-40
3.67E-58
2.42E-14
4.59E-37
5.69E-15
1.09E-32
8.72E-25
1.89E-59
1.39E-218
1.39E-218
3.46E-212
2.36E-211
3.92E-206
2.22E-178
3.14E-21
9.65E-11
5.24E-09
1.41E-27
9.20E-16
9.39E-25
1.57E-15
8.40E-33
4.45E-49
5.44E-86
0.00000139
2.06E-15
Module enrichments by KEGG
module
KEGG.name
black
blue
brown
cyan
darkgreen
darkgrey
darkred
darkturquoise
green
greenyellow
grey60
lightcyan
lightgreen
lightgreen
lightgreen
lightgreen
lightgreen
lightgreen
lightyellow
magenta
midnightblue
orange
pink
purple
red
royalblue
salmon
tan
turquoise
yellow
Ascorbate and aldarate metabolism
Lysosome
PPAR signaling pathway
Parkinson's disease
Bile secretion
Ribosome
Glycosaminoglycan biosynthesis - chondroitin sulfate
Type II diabetes mellitus
SNARE interactions in vesicular transport
NOD-like receptor signaling pathway
Proteasome
Ribosome
Cell cycle
DNA replication
Oocyte meiosis
Mismatch repair
Progesterone-mediated oocyte maturation
Homologous recombination
Dorso-ventral axis formation
Bile secretion
Pantothenate and CoA biosynthesis
Glycosaminoglycan degradation
MAPK signaling pathway
Thyroid cancer
Ubiquinone and other terpenoid-quinone biosynthesis
Spliceosome
Pancreatic secretion
ECM-receptor interaction
Neuroactive ligand-receptor interaction
Ubiquitin mediated proteolysis
p.value
0.0000481
5.73E-15
8.09E-08
3.20E-54
8.50E-10
9.98E-29
0.000148
0.00000609
0.0000185
6.52E-22
0.000000172
1.56E-75
6.76E-80
3.94E-65
7.49E-22
1.63E-18
1.22E-17
7.98E-16
9.63E-12
0.000983
2.16E-13
8.15E-16
0.00133
7.29E-08
0.00000085
2.40E-08
6.03E-39
9.15E-36
0.000207
1.46E-08
% variance explained by module eigengenes
0.6
0.5
0.4
0.3
0.2
0.1
0
Architecture of module eigengenes
Architecture of MElightgreen and islet D2O incorporation
Correlation between MEs and all clinical traits
Correlation between MEs and all clinical traits
Correlation between MEs and all clinical traits
Search for circulating molecules stimulating b-cell replication
Properties of candidates:
Secreted factors
Correlated to islet D2O
Located at cell cycle loci
e.g., Igfals in adipose
- secreted
- correl to islet D2O = 0.48
- correl to insulin = 0.58
- cis eQTL on Chr17
- coding SNP (Sanger)
Igfals KO mice
Igfals expression in our database:
Are circulating IGF levels different?