Curriculum
vitae
Personal information
Name
Current Address
Telephones
E-mail
Nikolaos Ioannidis
University of Crete, Department of Biology, Vassilika Vouton P.O. box 2208 P.C.
71409 Greece
Work: +302810394068
Mobile: +306944275081
[email protected]
Specialization
Bioenergetics, Proton motive force, Photosynthesis, Respiration, Time resolved
fluorescence and absorbance spectroscopy, Non destructive in vivo probing, Cell
cultures, UV-B stress in photosynthetic eukaryotes (unicellular algae & higher
plants)
Degrees
B. Sc. in Biology (University of Crete, Greece, 1999), M.Sc. in Plant Molecular
Biology and Biotechnology (UoC, 2001) and Ph. D (UoC, 2006).
Appointments
Dates
Main activities and
responsibilities
Name and address of employer
Dates
Occupation or position held
Main activities and
responsibilities
Name and address of employer
Page 1 - Curriculum vitae of
Nikos Ioannidis
1/2010– present
Post-doctoral Research
The role of spermidine and spermine in the partitioning of proton motive force in
thylakoids of intact plants
3/2007 – 9/2009
Adjunct Lecturer in Department of Biology (UoC)
Post-doctoral Research and lecturing (Biochemistry I and Biochemistry II)
Department of Biology, University of Crete, Heraklion, Greece, P.C. 71409
Personal skills and
competences
Language(s)
Techniques
English, German
In vivo time-resolved fluorescence spectroscopy (sampling rate up to 100kHz)
(OJIP curve, NPQ, qE, qT, qI, PSIIα & PSIIβ)
In vivo time-resolved absorbance spectroscopy (ECSt, ECSinv, gH+, ΔpH/pmf,
Δψ/pmf)
Fluorescence spectroscopy (RT and 77K)
UV-Vis spectroscopy
Biochemical Analytical techniques (plus Ultracentrifugation)
HPLC maintainance and operation (High Perfomance Liquid Chromatography)
Polarography
Molecular Biology methods (SDS page, western blot, DNA isolation, gel
electrophoresis)
Cell cultures (unicellular algae)
In vitro plant cultures
Software
Use of commercially available software for spectrophotometers, HPLC,
fluorometers
Biolyser (analysis of OJIP curves)
Origin
Swiss-Pdbviewer/ Deep View
Scripting in Visual basic (minor experience)
Career break
Reason
Dates
Compulsory Military Service (Greek Army)
5/2006 – 2/2007
Fellowships
Dates
Type of scholarship earned
Name and type of organisation
providing the scholarship
6/2008-9/2008
Short Term Fellowship (90 days)
European Molecular Biology Organization, EMBO
Host
Prof. David Kramer, Institute of Biological Chemistry, Pullman, Washington State,
USA
Project
The effect of putrescine on the thylakoidal proton motive force in vivo and in vitro
Dates
Type of scholarship earned
Page 2 - Curriculum vitae of
Nikos Ioannidis
10/2007 and 11/2009
Short Term Fellowship (about two months in total)
Name and type of organisation
providing the scholarship
Host
Project
Dates
Type of scholarship earned
Name and type of organisation
providing the scholarship
Host
Project
Dates
Type of scholarship earned
Name and type of organisation
providing the scholarship
Supervisor
PhD Thesis title
Dates
Type of scholarship earned
Name and type of organisation
providing the scholarship
Post graduate programme
coordinator
Exchange Programme
Erasmus
Name and type of organisation
providing education and
training
Page 3 - Curriculum vitae of
Nikos Ioannidis
IKYDA (Greek State Scholarship Foundation and German Academic Exchange
Service)
Prof. Klaus Humbeck Martin-Luther Univ. Halle-Wittenberg, Germany
Polyamine changes in cellular and plastidal level during plant senescence
8/2003 and 8/2004
Short Term Fellowship (about two months in total)
IKYDA (Greek State Scholarship Foundation and German Academic Exchange
Service)
PD Dr Dieter Dörnemann, Phillips University Marburg, Germany
Study of the methanol effect in photosynthesis; possible biotechnological
applications
2002 -3/2006
“Heraklitos” scholarship for doctoral studies
Greek Ministry of education
Prof. K. Kotzabasis (Department of Biology, University of Crete, Greece)
A comparative study of light dependent and light independent biogenesis and
functional assembly of the photosynthetic apparatus and the role of polyamines
2000-2001
Scholarship for excellent performance during postgraduate studies
Greek Ministry of Education and European Union 6th Framework programme
Prof. K. Roubelakis-Aggelakis (Department of Biology, University of Crete, Greece)
One semester scholarship (1/1999-6/1999)
School of Biological Science, University of Manchester, Manchester, U.K.
Participation as researcher in projects (Photobiology Laboratory, UOC, Prof. K. Kotzabasis)
1. Functional characterization Nicotiana tabacum overexpressing plastidial tranglutaminase of Zea Mays (2008-2010)
2. Functional characterization of Arabidopsis thaliana adc2-2 mutant (2008-2010)
3. In vivo probing of endemic plants of the Cretan area by non destructive means (2008)
4. Two year program for Phylogeography and Ecophysiology of Campanulaceae and Dispacaceae in the Cretan area.
Cooperation between the Natural History Museum of Crete (University of Crete) and Peabody Natural History Museum
(University of Yale) (2003-2004).
Other activities
Reviewer for :
Photosynthesis Research (from 2008)
Research Interests
Bioenergetics, proton motive force, photoprotection, stress, Photosystem II, qE,
Light Harvesting Complex II, ATPase, in vivo probes, spectroscopic tools
List of publications
1. Ioannidis N, Ortigosa S, Veramendi J, Pintó-Marijuan M, Fleck I, Carvajal P, Kotzabasis K, Santos M, Torné JM
(2009) Remodeling of tobacco thylakoids by over-expression of maize plastidial transglutaminase
Biochimica et Biophysica Acta (BBA)-Bioenergetics 1787: 1215-1222 (IF 4.447)
2. Sfichi L, Ioannidis N, , Kotzabasis K (2008) Fast and reversible response of thylakoid-associated polyamines during
and after UV-B stress – a comparative study of the wild type and a mutant lacking chlorophyll b of unicellular green alga
Scenedesmus obliquus.
PLANTA 228: 341-353 (IF 3.088)
3. Ioannidis N, and Kotzabasis K. (2007). Effects of polyamines on the functionality of photosynthetic membrane in vivo
and in vitro.
Biochimica et Biophysica Acta (BBA) – Bioenergetics 1767: 1372-1382 (IF 4.447)
4. Navakoudis E., Ioannidis N, Dörnemann D. and Kotzabasis K. (2007) Changes in the LHCII-mediated energy
utilization and dissipation adjust the methanol-induced biomass increase.
Biochimica et Biophysica Acta (BBA) – Bioenergetics 1767: 948-955 (IF 4.447)
5. Ioannidis N, Sfichi L, Kotzabasis K (2006) Putrescine stimulates chemiosmotic ATP synthesis.
Biochemica et Biophysica Acta (BBA)- Bioenergetics 1757: 821-828 (IF 4.447)
6. Sfichi L, Ioannidis N, Kotzabasis K (2004) Thylakoid-associated Polyamines Adjust the UV-B Sensitivity of the
Photosynthetic Apparatus by Means of Light-harvesting Complex II Changes.
Photochemistry and Photobiology 80: 499-506 (IF 2.287)
7. Logothetis K, Dakanali S., Ioannidis N. , Kotzabasis K. (2004) The impact of high CO2 concentrations on the structure
and function of the photosynthetic apparatus and the regulatory role of polyamines.
Page 4 - Curriculum vitae of
Nikos Ioannidis
Journal of Plant Physiology 161: 715-724 (IF 2.437)
Manuscripts at final stage of preparation/submitted
8. A putrescine deficient mutant of Arabidopsis shows altered partitioning of thylakoidal proton motive force into ΔpH and
electric field (Δψ) Ioannidis N, Cruz J, Kotzabasis K, DM Kramer
9. Ioannidis N, Tsiavos T, Kotzabasis K Chemical bonding of chlorophylls and plant aminic axial ligands impact
harvesting of visible light and quenching of fluorescence submitted PRES1236
Chapters in books
Kagiampakis A, Ioannidis N. Damanakis M. (2010) The current status of Lomelosia minoana ssp. Asterusica, New Red
Data Book For Rare And Threatened Plants Of Greece, Editors D. Phitos, T. Konstandinidi and G. Kamari.
Monographies
The role of polyamine during light dependent and light independent conversion of protochlorophyllide to chlorophyllide
(Master thesis).
A comparative study of light dependent and light independent biogenesis and functional assembly of the photosynthetic
apparatus and the role of polyamines (PhD thesis)
List of International conference presentations (poster)
Ortigosa S, Ioannidis N, Veramendi J, Pintó-Marijuan M, Fleck I, Carvajal P, Kotzabasis K, Santos M, Torné JM
Remodeling of tobacco thylakoids by over-expression of maize plastidial transglutaminase (ΧΧΙΙ Conference for Plant
Physiologist 2009, Spain)
The role of polyamines during induction of photosynthesis. Ioannidis N and Kotzabasis K. (International Botanical
Congress XVII, Vienna, Austria 2005)
In situ physicochemical measurements of the Cretan endemic genus Petromarula evaluate its adaptability to the certain
environmental conditions of Crete. Ioannidis N, Navakoudis E, Avramakis M, Hatzinikolakis E and Kotzabasis K.
(International Botanical Congress XVII, Vienna, Austria 2005)
Application of fluorescence induction parameters to assess the environmental adaptability of endemic plants on the
island of Crete. Navakoudis E, Ioannidis N, Avramakis M, Hatzinikolakis E and Kotzabasis K. (International Botanical
Congress XVII, Vienna, Austria 2005)
The role of polyamines during the short-term light adaptation of the photosynthetic apparatus in dark grown
Scenedesmus cultures. Ioannidis N. and Kotzabasis K. (14th FESPP Cracow, Poland 2004)
The CO2 effect on the photosynthetic apparatus and the role of polyamines Ioannidis N., Logothetis K., Dakanali S., and
Kotzabasis K. (5th Workshop of Microalgal Biotechnology, Berlin, Germany 2003)
Photoregulation of methanol induced microalgal growth and the photosynthetic adaptive response. Ioannidis N.,
Navakoudis, A. Theodoridou, D. Dörnemann and K. Kotzabasis (International Plant Photobiology Meeting, Marburg,
Germany 2003).
The regulatory role of polyamines on the light-independent protochlorophyllide/chlorophyllide conversion in the mutant C2A’ of Scenedesmus obliquus. Ioannidis N and Kotzabasis K. (13th FESPP Congress 2002, Heraklion, Greece.)
Page 5 - Curriculum vitae of
Nikos Ioannidis
Invited Speaker
Light and variable fluorescence: “Trojan horse” for the understanding of plant cell. Departament de Genètica Molecular,
CRAG Centre for Research in Agricultural Genomics, CSIC- UAB- IRTA, Barcelone 2 December 2008
Page 6 - Curriculum vitae of
Nikos Ioannidis