Spooling DNA
Source: Strawberries
Octoploid (8 copies of their genomic DNA)
DNA Extraction

Place strawberry in baggie and close baggie

“mush” berry for about 2 min.

Add DNA extraction buffer

Close baggie, and “mush” berry again for 2 min.
DNA Extraction

Set-up beaker with cheese cloth

Cut corner of baggie and pour strawberry solution into cheese cloth

Squeeze out solution into beaker, the waste/pulp is trapped in cheese
cloth

The strawberry solution contains your DNA
DNA Extraction

Fill the test tube 1/3rd to ½ with the strawberry solution.

Now very slowly using the pipet add ice cold ethanol down the
side of the test tube. Add enough until the tube is about 3/4th filled
and you should see a whitish material. You want two layers to form.

Now take the wooden stick and begin “spooling”. Place the stick in
near the interface of ethanol and the strawberry solution and “twirl”.

DO NOT MIX ETHANOL WITH THE STRAWBERRY SOLUTION.

You have now isolated genomic DNA from strawberries!