Title: Management Tools for Algal Leaf Spot
Protocol #: 17-016
Draft
p 1 of 3
Objective: Determine efficacy of new active ingredient formulations and new biopesticides for managing algal
leaf spot diseases.
Experimental Design:
Plot Size: Must be adequate to reflect actual use conditions.
Replicates: Minimum of 6.
Target Species: TBD. Contact your regional coordinator if additional target species are of interest.
Plant Hosts: Use a plant host suitable for target species, recording species and variety used.
Treatments: See Treatment Table on next page. Test materials should have been stored for no more than 2
years prior to start of experiment. Biological test materials should be requested for arrival approximately
2 weeks prior to first application unless otherwise directed by manufacturer.
Application Instructions: Apply each treatment according to directions in the table below. All treatments
are foliar applications. Applications should be made using application equipment consistent with
conventional commercial equipment. Calibrate application equipment prior to application. Read
available labels before making applications. Avoid treating plants under unusually extreme
environmental conditions.
Use Site: May be greenhouse, field container or field in-ground. Please specify in final report.
Evaluations: Record disease severity and incidence 0, 7, 14, 21 and 28 days after initial application.
Evaluate phytotoxicity at same time points. Record phytotoxicity as chlorosis, defoliation, discoloration,
stunting or other growth effects on a scale of 0 to 10 (0 = No effect; 10 = Complete plant affected). If
stunting is observed, record plant height at last evaluation point. If any phytotoxicity is observed in
treated plants, take pictures comparing treated and untreated plant material.
Maintenance Applications: Applications during an experiment to manage non target diseases, pests or weeds
may be needed to appropriately evaluate efficacy or crop safety. Because of potential negative impacts
due to simultaneous or sequential applications of certain products with test materials please avoid
applying the following: TBD.
If applications must be made of any products on the above list, please contact IR-4 prior to application.
Recordkeeping:
All operations, data and observations appropriate to this study should be recorded. It is helpful to review the
Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions
including temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low
and average daily temperatures. Other information to record includes soil-type or soil-less media,
application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth
stage at application and data collection dates. Content of reports should be sufficient to fully understand how
the experiment was conducted.
Photographs often illustrate experimental design, site conditions, and impacts of treatments very well. It is
encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It
is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are
visually apparent.
If different application methods or evaluations are made, please clearly specify differences in final report
and explain reason for change.
Treatments:
Priority
A
#
1
2
6
7
8
Milstop (potassium bicarbonate)
Mural (azoxystrobin + benzovindiflupyr)
Optional: Mural
9
OxiPhos (mono- and di-potassium salts of phosphorus acid
+ hydrogen peroxide)
Optional: OxiPhos
10
Zerotol 2.0 (hydrogen dioxide + peroxyacedic acid)
Optional Zerotol 2.0
Controls
Nontreated Noninoculated
Nontreated Inoculated
Standard
Rendition
Optional: Rendition
Key: A = Application; I = Inoculation; e = Evaluation
1.5 to 2.5
lb
7 oz
4 oz
7
e/A
e/A
e/A
e/A
e/A
e/A
e/A
e/A
e/A
e
e
e
e
e
e
e/A
e/A
e/A
e
e
e/A
e/A
e/A
e/A
e/A
e/A
e
e
e
e
e/A
e/A
e/A
e
e
e
e
e/A
e/A
e
e
e/A
e/A
e
e
e/A
e/A
e
e
e
e
e
e
e
e
21
e
e
e
28
e
e
e
2 gal
1 gal
2 gal
1 gal
--3 pints
1 pint
Title: Management Tools for Algal Leaf Spot
5
0
e/A
e/A
e/A
e/A
e/A
e/A
e/A
e/A
e/A
Days
14
e/A
e/A
e/A
Protocol #: 16-016
3 4 (Pick 2)
Product
Camelot O (copper octanoate)
COC DF (copper oxychloride)
Cuproxat (basic copper sulfate)
Daconil Ultrex (chlorothalonil)
Daconil Weather Stik (chlorothalonil)
Daconil Zn (chlorothalonil)
Double Nickel LC (Bacillus amyloliquefaciens strain D747)
Optional: Double Nickel LC
GC Pro (sodium carbonate peroxyhydrate)
Optional: GC Pro
Rates (per
100 gal)
1.0 gal
1.0 lb
15.5 oz
1.2 lb
20 fl oz
32 fl oz
4 qt
2 qt
4 lb
Final
p 2 of 3
Title: Management Tools for Algal Leaf Spot
Protocol #: 16-016
Final
p 3 of 3
Sources for Products:
When placing requests for samples, please ensure you include the amount needed, the first planned application
date, and shipping address and phone number.
Product
Camelot O
COC
Daconil Ultrex,
Weather Stik, Zn
Double Nickel
GC Pro
Milstop
Mural
OxiPhos
Rendition
ZeroTol
Contact
SePro, Mark Brotherton
Albaugh, Morris Gaskins
Phone
229-244-3288
Email
[email protected]
[email protected]
Syngenta, Steve Cosky
336-632-7148
[email protected]
Certis, Mike Dimock
BioSafe Systems, Vijay
Choppakatla
BioWorks, Matt Krause
Syngenta, Steve Cosky
BioSafe Systems, Vijay
Choppakatla
Certis, Mike Dimock
BioSafe Systems, Vijay
Choppakatla
301-483-3801
[email protected]
860.290.8890 ext. 221
[email protected]
585-924-4362 x335
336-632-7148
[email protected]
[email protected]
860.290.8890 ext. 221
[email protected]
301-483-3801
[email protected]
860.290.8890 ext. 221
[email protected]
Reports:
Reports are due 60 days after last data collection date.
Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The
basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic
documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be
submitted as picture embedded in the report or as separate jpg, bmp, or tiff files.
Please direct questions to:
Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540,
Phone 732-932-9575 x4629, [email protected].
OR
Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: 410-923-4880, E-mail:
[email protected].
Draft Date: 11/29/2016
Revised By: CLP