40
40
35
21
△ miR-21
106b
× miR-106b
■ let-7a
let-7a
30
Ct value
30
25
20
20
15
10
10
5
00
-5
-5
-4
-4
-3
-3
-2
-2
-1
-1
0
0
Log concentration
1
1
Concentrations (amol/μl)
A: miR-21
1.00E+01
1.600E-02
8.00E-1
4.000E-03
2.00E-1
Concentrations (amol/μl)
5.000E-03
2.50E-1
B: miR-106b
1.00E+01
1.200E-02
6.00E-1
1.00E-1.1
3.000E-03
1.50E-1
1.00E-1.1
8.000E-03
4.00E-1
2.000E-03
1.00E-1
1.00E-2
.01
1.000E-03
5.00E-2
0
0.000E+00
1E-3
1.00E-3
MK
normal
cancer
cancer (n=34)control (n=15)
3.000E-03
Concentrations (amol/μl)
1.50E-1
control
1.00E+01
C: let-7a
2.500E-03
1.25E-1
2.000E-03
1.00E-1
1.00E-1.1
1.500E-03
7.50E-2
1.000E-03
5.00E-2
1.00E-2
.01
5.000E-04
2.50E-2
00.000E+00
1.00E-2
.01
4.000E-03
2.00E-1
1.00E-3
1E-3
cancer (n=34)control (n=15)
MK
normal
cancer
control
0.000E+00
0
1E-3
1.00E-3
cancer (n=34)control (n=15)
MK
normal
cancer
control
p=0.013
miR-21 Concentrations (amol/μl)
1.00E-1
1.000E-01
miR-106b Concentrations (amol/μl)
1.00E-1
1.000E+00
1.20E-1
1.200E-01
8.00E-2
8.000E-01
8.00E-2
8.000E-02
6.00E-2
6.000E-01
6.00E-2
6.000E-02
4.00E-2
4.000E-01
4.00E-2
4.000E-02
2.00E-2
2.000E-01
2.00E-2
2.000E-02
0
0.000E+00
p=0.022
Pre-Op
1 month post -Op
0
0.000E+00
Pre-Op
1 month post -Op
1E-3
1.00E-3
cancer
MK
箱ヒゲ図 patient
normal
controls
3.00E-2
.03
3E-3
3.00E-3
.05
5.00E-2
ｎormal
controls
cancer
cancer
patient
controls
2.00E-1.2
let-7a濃度
let-7a Concentrations (amol/μl)
m i R - 1 0 6 a 濃度
miR-106b Concentrations (amol/μl)
.5
5.00E-1
cancer
cancer
patient
ｎormal
controls
群分け変数： cancerかnormal
群分け変数： cancerかnormal
.01
5.00E-3
cancer
cancer
箱ヒゲ図 patient
.02
2.00E-2
2E-3
2.00E-3
ｎormal
1.00E+01
1.00E-1.1
m i r 1 0 6 a 濃度
1.00E-2.01
miR-106a Concentrations (amol/μl)
miR-21 Concentrations (amol/μl)
17-5p濃度
1.00E-1.1
.3
3.00E-1
miR-21濃度
miR-17-5p Concentrations (amol/μl)
1.00E+01
1.00E-4
1E-4
群分け変数： MKかnormalか
群分け変数： cancerかnormal
群分け変数： MKかnormalか
.01
1.00E-2
1.00E-3
1E-3
1.00E-4
1E-4
cancer
MK
patient
normal
controls
1
1.0
Sensitivity
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
00
miR-106b
AUC=0.721
00
0.2
0.2
0.4
0.6
0.8
0.4
0.6
0.8
1-Specificity
1.0
1
1.01
Sensitivity
0.8
0.8
0.6
0.6
0.4
0.4
0.2
0.2
00
miR-106a/let-7a
AUC=0.879
00
0.2
0.2
0.4
0.6
0.8
0.4
0.6
0.8
1-Specificity
1
1.0
1
0.8
0.8
Sensitivity
Sensitivity
1
0.6
0.4
miR-17-5p
AUC=0.626
0.2
0.4
miR-21
AUC=0.673
0.2
0
0
0
0.2
0.4
0.6
0.8
1
1-Specificity
0.8
0.6
0.4
miR-106a
AUC=0.634
0.2
0
0
0.2
0.4
0.6
1-Specificity
0
0.2
0.4
0.6
1-Specificity
1
Sensitivity
0.6
0.8
1
0.8
1
1
0.8
0.8
Sensitivity
Sensitivity
1
0.6
0.4
miR-17-5p/let-7a
AUC=0.784
0.2
0.4
miR-21/let-7a
AUC=0.753
0.2
0
0
0
0.2
0.4
0.6
1-Specificity
0.8
1
1
0.8
Sensitivity
0.6
0.6
0.4
miR-106b/let-7a
AUC=0.776
0.2
0
0
0.2
0.4
0.6
1-Specificity
0.8
1
0
0.2
0.4
0.6
1-Specificity
0.8
1
Table 1
Expression of mature miRNAs in gastric cancer tissue versus those in normal tissue
miR-106b
let-7a
case 1
1.344
0.722
case 2
case 3
case 4
case 5
case 6
case 7
case 8
1.357
1.241
0.346
3.812
9.966
1.608
2.897
0.681
0.880
0.515
3.659
0.129
0.139
0.260
Rate of higher expression level
in gastric cancer tissue
87.5%
12.5%
Rate of lower expression level
in gastric cancer tissue
12.5%
87.5%