DOC/LP/01/28.02.02
LP- BT2251
LESSON PLAN
LP Rev. No: 00
Sub Code & Name : BT2251 Basic Industrial Biotechnology
Date: 15/12/2010
Unit: 1
Page 1 of 6
Branch: BIOTECHNOLOGY Semester : IV
Unit syllabus: INTRODUCTION TO INDUSTRIAL BIOPROCESSES
A historical overview of industrial fermentation process-traditional and modern
biotechnology. A brief survey of organisms, processes, products related to modern
biotechnology. Process flow sheeting-block diagrams, pictorial representations.
Objective: To introduce the Basic concepts of fermentation technology.
Session
No
Topics to be covered
1
Historical overview of industrial fermentation
products.
2
Comparison between traditional and modern
methods of fermentation.
3
Industrially useful microorganisms and its
products.
4
Basic design of the Fermentor
5
Overview of Fermentation processes
6
Process flow sheeting-block diagrams and flow
sheets.
7
Review
Time
min
50
Ref
TB1 (7-13)
TB 2 (14-19)
TB1 (14-20)
50
TB1 (21-22)
50
TB1 (25-42)
50
TB1 (42-59)
50
50
TB3 (74-80)
Teaching
Aids
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
DOC/LP/01/28.02.02
LP- BT2251
LESSON PLAN
LP Rev. No: 00
Sub Code & Name : BT2251 Basic Industrial Biotechnology
Date: 15/12/2010
Unit: 2
Page 1 of 6
Branch: BIOTECHNOLOGY Semester : IV
Unit syllabus: PRODUCTION OF PRIMARY METABOLITES
A brief outline of processes for the production of commercially important organic acids
(Citric acid, lactic acid, acetic acid etc.), amino acids (Glutamic acid, phenylalanine,
aspartic acid etc.) and alcohols (ethanol, Butanol etc.)
Objective: To discuss in detail the production of primary metabolites.
Session
No
Topics to be covered
Time
min
Ref
Teaching
Aids
8
Citric acid production.
50
TB1 (403-407)
BB/OHP
9
Lactic acid production
50
TB1 (304-313)
BB/OHP
10
Acetic acid production and outline of process
for production of other commercially
important organic acids.
50
TB1 (333-343)
BB/OHP
11
Glutamic acid production
50
12
Phenylalanine production
50
TB1 (151-157)
BB/OHP
13
Aspartic acid production and outline of
process for production of other commercially
important amino acids
50
TB3 (150-156)
BB/OHP
14
Ethanol production
50
TB3 (299-303)
BB/OHP
15
Butanol production
50
TB1 (260-273)
BB/OHP
16
Outline of process for production of other
commercially important alcohols.
50
TB1 (277-283)
BB/OHP
17
Review
TB1 (7-13)
BB/OHP
DOC/LP/01/28.02.02
LESSON PLAN
LP- BT2251
LP Rev. No: 00
Sub Code & Name : BT2251 Basic Industrial Biotechnology
Date:15/12/2010
Unit: 3
Page 3 of 6
Branch: BIOTECHNOLOGY Semester : IV
Unit syllabus: PRODUCTION OF SECONDARY METABOLITES
Study of production processes for various classes of secondary metabolites: antibiotics,
beta-lactams (penicillin, cephalosporin etc), amino glycosides (streptomycin), macrolides
(erythromycin), vitamins and steroids.
Objective: To impart knowledge, to discuss in detail the production of secondary
metabolites.
Session
No
19
20
21
22
23
Topics to be covered
Overview of production process of
antibiotics.
TB1 (223-247)
50
Cephalosporin production
50
Streptomycin production.
Overview of production process of
macrolides
25
Overview of production process of
vitamins
28
TB3 (240-243)
TB1 (248-254)
Production of erythromycin.
27
Teaching Aids
Ref
TB1 (221-223)
50
Production of penicillin.
24
26
Time
min
Production of commercially
important vitamins.
50
50
50
Review.
BB/OHP
BB/OHP
BB/OHP
TB3 (258-260)
BB/OHP
TB1 (337-350)
50
TB1 (347-350)
50
50
BB/OHP
TB3 (256-257)
TB1 (350-389)
Production process of steroids.
BB/OHP
BB/OHP
BB/OHP
BB/OHP
DOC/LP/01/28.02.02
LP- BT2251
LESSON PLAN
LP Rev. No: 00
Sub Code & Name : BT2251 Basic Industrial Biotechnology
Date: 15/12/2010
Unit: 4
Page 4 of 6
Branch: BIOTECHNOLOGY Semester : IV
Unit syllabus: PRODUCTION OF ENZYMES AND OTHER BIOPRODUCTS.
Production of industrial enzymes such as proteases, amylases, lipases, cellulases.
Production of biopesticides, biofertilizers, biopreservatives (nisin), cheese, biopolymers
(xanthan gum, PHB), single cell protein.
Time
Session
Teaching
No
Topics to be covered
Ref
Aids
29
Production of Proteases & amylases
30
Production of lipases & cellulases
31
Production of biopesticides
32
Production of biofertilizers.
33
Production of Nisin and cheese.
34
Production of xanthan gum & PHB
35
Production of SCP.
36
Review.
TB1 (395-398)
50
TB1 (400-402)
50
50
50
50
50
50
BB/OHP
BB/OHP
TB3 (301-304)
BB/OHP
TB3 (219,286)
BB/OHP
TB3 (231,247)
BB/OHP
TB3 (70,330)
BB/OHP
TB3 (69-73, 306-313)
BB/OHP
DOC/LP/01/28.02.02
LP- BT2251
LESSON PLAN
LP Rev. No: 00
Sub Code & Name : BT2251 Basic Industrial Biotechnology
Date: 15/12/2010
Unit: 5
Page 5 of 6
Branch: BIOTECHNOLOGY Semester : IV
Unit syllabus: PRODUCTION OF MODERN BIOTECHNOLOGY PRODUCTS
Production of recombinant proteins having therapeutic and diagnostic applications,
production of vaccines, production of monoclonal antibodies, products of plant and
animal cell culture.
Session
No
Time
Topics to be covered
Ref
Teaching
Aids
min
TB4 (227-243)
37
Production of recombinant proteins. I
38
Production of recombinant proteins .II
39
Production of recombinant proteins .III
40
Production of vaccines I
41
Production of Vaccines II
42
Production of monoclonal antibodies
43
Products of plant cell culture
44
Products of animal cell culture.
50
45
Review
50
46
Final review.
50
TB4 (227-243)
50
TB4 (227-243)
50
TB4 (286-298)
50
TB4 (286-298)
50
50
50
TB4 (286-298)
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
BB/OHP
TB3 (231-232)
BB/OHP
TB3 (145-147)
BB/OHP
Course Delivery Plan:
Week
Units
Week
1
2
3
4
5
6
7
8
9
10
11
12
13
III
I
III
III
II
III
III
III
III
III
III
III
III
IV
III
III
III
14
III
15
III
V
Units
Textbooks:
1. Casida Jr,L.E,”Industrial microbiology”New age international Ltd.
2. Presscott,Dunn,”Industrial Microbiology”,Agrobios (India)
References:
3. Wulf Cruger and Anneliese crueger”Biotechnology:a text book of industrial
Microbiology”, panima publishing corporation.
4. Murrey Moo & Young” Comprehensive Biotechnology”, Pergamon.
5. A.H.Patel,”Industrial Microbiology”, Macmillan publishers India Ltd, Agriculture and
Life Sciences.
Prepared by
Approved by
N. Sathish
Prof. M. Sivanandham
Asst.Professor
Head of the Department
15/12/2010
15/12/2010
Signature
Name
Designation
Date