Supporting information Figures S1-S5
Supplemental figure 1
100
90
%
80
70
F
60
M
50
A
40
m
30
a
20
10
0
P+_S+
P+_S-
P-_S+
P-_S-
Figure S1: Mycorrhizal colonization parameter et al 1996
Mycorrhizal colonization parameters were estimated according to Trouvelot et al. 1986. Data
shown are average data of all three independent experiments (spring and autumn 2010 and
spring 2012) +/- standard deviation.
Supplemental figure 2
180
cpm/ mg fresh weight
160
140
120
100
pot#1 nm
80
pot#2 nm
60
pot#1 m
40
pot#2 m
20
0
0
1
days after application
2
Figure S2: Uptake and transport of 35S-sulfate by Glomus intraradices.
Medicago truncatula plants inoculated or not with Glomus intraradices, were grown in twocompartment systems for five weeks. 100 µCu 35S-labeled Na-sulfate was applied to the fungal
compartment and the radioactivity in the plant leaves was measured one and two days after
application.
Supplemental figure 3
A
B
SULTR1
SULTR1;1
SULTR1;2
SULTR1;3
MtrStr1.1
SULTR2;1
SULTR2;2
MtrStr2.1a
MtrStr2.1b
MtrStr2.2
SULTR3;1
MtrStr3.1
SULTR3;2
MtrStr3.2
SULTR2
SULTR3
MtrStr1.3
SULTR3;3
SULTR3;4
SULTR3;5
SULTR4
SULTR4;1
SULTR4;2
MtrStr4.1
500 bp
UTR
Exon
Intron
Figure S3: Phylogenetic relationship of SULTR proteins and genomic organization of SULTR gene
family.
(A) A Maximum-Likelihood Tree based on a Clustal W alignment was calculated using the MEGA 5.03
program (Tamura et al, 2011) with selected Sulfate Transporter Protein Sequences. Reference
numbers for the corresponding nucleotide sequences are given in brackets. Arath = Arabidopsis
thaliana, Mtr = Medicago truncatula. (B) Exon-intron structures of SULTR genes. The untranslated
region (UTR) sequences are shown when available. Open boxes represent UTRs; solid boxes, coding
regions; and solid lines, introns.
Supplemental Figure 4
PC2: 40%
+P+Snm
+P+Sm
+P
+P-Sm
PC1: 49%
+P-Snm
-P-Sm
-P-Snm
-P+Sm
-P+Snm
-P
+P+Snm
+P-Snm
+P+Sm
+P-Sm
-P+Snm
-P-Snm
-P+Sm
-P-Sm
Figure S4. PCA (Principal Component Analysis) score plot of metabolite profile.
The plots were applied for contents of 27 metabolites, total soluble protein and fresh weight per
plant. PC: principal component.
Supplemental Figure 5
Figure S5: Heat map representing metabolite changes per plant of mycorrhizal and non-mycorrhizal
plants under different nutritional conditions.
The heatmap shows the changes in the metabolite profile of M. truncatula plants under different
nutritional conditions. The plants were mycorrhizal colonized (myc) or not (nm) and grown under +/- P
and +/-S conditions. Metabolite contents per plant were obtained from leaf samples of 5-week-old
plants. Data was normalized on the average level in all conditions. The data with log2 scale was
submitted to Hierarchical Clustering Analysis (HCA) using Pearson correlation to identify clusters of
similar metabolic profiles. Cells with high abundance are presented by red colour and cells with blue
colour show lower abundance with the scale above heat map.