Annexin A2 is involved in antiphospholipid antibodymediated pathogenic effects in vitro and in vivo
by Zurina Romay-Penabad, Maria Guadalupe Montiel-Manzano, Tuya Shilagard,
Elizabeth Papalardo, Gracie Vargas, Arun B. Deora, Michael Wang, Andrew T.
Jacovina, Ethel Garcia-Latorre, Elba Reyes-Maldonado, Katherine A. Hajjar, and
Silvia S. Pierangeli
Blood
Volume 114(14):3074-3083
October 1, 2009
©2009 by American Society of Hematology
Analysis of antigen specificity of the monoclonal Ab preparations.
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Effect of anti-A2 on IgG-APS–induced expression of ICAM-1 and E-sel in HUVECs. HUVECs were
cultured and treated with either 200 μg/mL IgG-APS or 200 μg/mL IgG-NHS in the presence or
absence of 1 μg/mL anti-A2 IgG Ab or 1 μg/mL MuMoAbC, as indicated in “In v...
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Effect of anti-A2 Ab on TF activity induced by IgG-APS in HUVECs. HUVECs were cultured and
treated with either IgG-APS or IgG-NHS in the presence or absence of anti-A2 MoAb, as
indicated in “TF functional assay.” Some cells were treated with LPS, as a posit...
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Effect of aPL Abs on thrombus formation in A2−/− mice.
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Effect of aPL Abs on TF activity in carotid artery homogenates in A2−/− mice.
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Aortic VCAM-1 expression in A2−/− and A2+/+ mice treated with IgG-APS.
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Representative images of endothelial VCAM-1 expression among the different treatment groups.
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology
Diagrammatic representation of interaction of aPL/anti-β2GPI Abs with β2GPI and receptor(s) on
ECs. aPL/anti-β2GPI Abs bind to domain I (DI) of β2GPI. β2GPI anchors to annexin A2 on the
surface of ECs, possibly through domain V (DV) of the protein.
Zurina Romay-Penabad et al. Blood 2009;114:3074-3083
©2009 by American Society of Hematology